Time-series sewage metagenomics distinguishes seasonal, human-derived and environmental microbial communities potentially allowing source-attributed surveillance.

Sewage metagenomics has risen to prominence in urban population surveillance of pathogens and antimicrobial resistance (AMR). Unknown species with similarity to known genomes cause database bias in reference-based metagenomics. To improve surveillance, we seek to recover sewage genomes and develop a quantification and correlation workflow for these genomes and AMR over time. We use longitudinal sewage sampling in seven treatment plants from five major European cities to explore the utility of catch-all sequencing of these population-level samples. Using metagenomic assembly methods, we recover 2332 metagenome-assembled genomes (MAGs) from prokaryotic species, 1334 of which were previously undescribed. These genomes account for ~69% of sequenced DNA and provide insight into sewage microbial dynamics. Rotterdam (Netherlands) and Copenhagen (Denmark) show strong seasonal microbial community shifts, while Bologna, Rome, (Italy) and Budapest (Hungary) have occasional blooms of Pseudomonas-dominated communities, accounting for up to ~95% of sample DNA. Seasonal shifts and blooms present challenges for effective sewage surveillance. We find that bacteria of known shared origin, like human gut microbiota, form communities, suggesting the potential for source-attributing novel species and their ARGs through network community analysis. This could significantly improve AMR tracking in urban environments.

Biochar application in organics and ultra-violet quenching substances removal from sludge dewatering leachate for algae production.

Algae production in nutrient rich sludge dewatering leachate after biogas production is a promising option for wastewater treatment plants. However, the ultra-violet (UV) absorbing characteristic of UV-quenching substances (UVQS) existing in these waters can notably reduce the light transmission within the liquid body. The present work demonstrates a comparative adsorptive removal of UVQS, and other organic substances (expressed as COD and TOC) onto the "acid catalyst" functionalised adsorbent (PPhA) and commercial activated carbon (CAC) from leachate originating from leftover sludge dewatering after biogas production. Laboratory scale column studies were performed to investigate the adsorption performance of selected parameters. The PPhA increased the UV transmittance of leachate more than 4 times and outperformed CAC. Bed Depth Service Time and Yan models were used on the experimental data in order to estimate the maximum adsorption capacity and evaluate the characteristics of the fixed-bed. The PPhA equilibrium uptake of COD and TOC amounted to 5.7 mg/g and 0.9 mg/g, respectively. The postulated removal mechanism in environmentally relevant conditions (e.g., pH neutral) suggested a complex interaction between the biochar and organic macromolecules. Diluted phosphoric acid solution (0.01 mol/L) was successfully used for the column regeneration. Beside the UVQS, PPhA showed affinity towards toxic heavy metals (e.g., Pb, Ni, Co) pointing out the rich surface chemistry of the PPhA. Based on the obtained results and successfully implemented scale-up methodology, the low-cost PPhA adsorbent might effectively compete with the CAC as a highly efficient platform in wastewaters leachate processing.