RESUMO
A method for bacterial culture storage and transportation is suggested consisting in impregnation of filter paper disks with broth culture and drying. Bacterial cultures can be stored in this mode for at least a year at 4 degrees C.
Assuntos
Técnicas Bacteriológicas , Manejo de Espécimes , PapelAssuntos
Aminopirina/farmacologia , Compostos de Anilina/farmacologia , Contaminação de Alimentos/análise , Indústria de Processamento de Alimentos/métodos , Temperatura Alta , Produtos da Carne/toxicidade , Carne/toxicidade , Peroxidase/toxicidade , Animais , Indústria de Processamento de Alimentos/normas , Indicadores e Reagentes , Produtos da Carne/análise , Peroxidase/análise , Controle de Qualidade , UcrâniaRESUMO
The suggested test for the identification of enterococci is based on their ability, unlike other streptococci, to grow in Levine's or Endo's media. A total of 128 enterococcal and 251 other streptococcal cultures were examined. The studies have demonstrated reliability of the suggested test, which permits, as a rule, cutting down the time for identification by 1 day and simplifying the investigation.
Assuntos
Meios de Cultura , Intestinos/microbiologia , Streptococcus/classificação , HumanosRESUMO
The passive hemagglutination test (PHAT) with a mixture of tested sera is suggested to be used in screening of HBsAg carriers. A pool of 4 sera is used in a test, diluted twofold with physiologic saline; thus the end dilution of each serum is 1:8. Examinations of 296 serum samples by the current and the 'group' methods have yielded the results that coincide. The 'group' PHAT method cuts down the consumption of the diagnostic agent 4-fold and is less labor-consuming.
Assuntos
Portador Sadio/imunologia , Testes de Hemaglutinação/métodos , Antígenos de Superfície da Hepatite B/análise , HumanosAssuntos
Bactérias/classificação , Nitrato Redutases/metabolismo , Bactérias/enzimologia , Nitratos , Nitritos , PapelRESUMO
Various nutrient bases (dry nutrient agar, erythrol agar, medium for toxigenicity determination, casein-yeast and AGV media) have been examined to choose a base for Pizu's medium instead of agar D that is no longer manufactured. AGV medium has been found the best nutrient base. The method for the preparation of Pizu's medium has been simplified. The modified Pizu's medium permits the detection of cystinase in C: diphtheriae in 3-5 hrs both in pure and mixed cultures.
Assuntos
Corynebacterium diphtheriae/isolamento & purificação , Meios de CulturaRESUMO
The method for the determination of the toxigenicity of C. diphtheriae by means of discs impregnated with diphtheria antitoxin is proposed. The discs can be prepared long before use and stored for a year. The determination of toxigenicity with these discs is not inferior in its sensitivity to the routine procedure of the immunoprecipitation test. The disc method decreases the consumption of unavailable diphtheria antitoxin by 10 times, that of culture media by 2-2.5 times: besides, it is less labor consuming.