RESUMO
The oligonucleotide primers for herpes simplex virus type 1 and 2 DNA detection are developed. In examined group of patients with genital herpes the virus of type 2 and type 1 was detected in 63% and 26% cases, respectively. The mixed infection of both types is revealed in 11% of the patients.
Assuntos
DNA Viral/genética , Herpes Genital/diagnóstico , Herpesvirus Humano 1/isolamento & purificação , Herpesvirus Humano 2/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Adulto , Primers do DNA , Feminino , Herpes Genital/virologia , Herpesvirus Humano 1/genética , Herpesvirus Humano 2/genética , Humanos , MasculinoRESUMO
A group of patients with ischemic heart disease (IHD), who underwent surgical aorta-coronary vascular shunting, was examined in this investigation. Low titers of HSV-1 specific IgG were detected in all patients, the obtained values being consistent with similar data obtained in healthy subjects of the same age. Negative PCR of HSV-I DNA in blood and biopsy results were obtained. None of the patients demonstrated typical clinical pattern of infectious disease caused by herpes simplex virus. These data are evidence of the absence of the HSV-1 correlation with coronary atherosclerosis in patients with the IHD diagnosis. The significance of HCMV specific IgG titers and HCMV DNA detected in blood plasma in 87.7% cases is probably attributed to existence of connection of HCMV infection markers revealing in patient' blood with IHD diagnosis and coronary atherosclerosis. Besides, the HCMV DNA presence in biopsy taken from myocardium or vascular wall with lesion is revealed in 100% cases. The cytomegalovirus markers in tissue lesions with the help of specific antiserums marked to HSMV recombinant proteins are also revealed in 100% cases. This fact indicates the connection between pathological atherosclerotical process in IHD and cytomegalovirus infection.
Assuntos
Biomarcadores/análise , Infecções por Herpesviridae/virologia , Isquemia Miocárdica/complicações , Adulto , Derivação Arteriovenosa Cirúrgica , Sangue/virologia , Citomegalovirus/genética , Citomegalovirus/imunologia , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/etiologia , Infecções por Citomegalovirus/virologia , DNA Viral/análise , Coração/virologia , Herpes Simples/etiologia , Herpes Simples/virologia , Infecções por Herpesviridae/etiologia , Herpesvirus Humano 1/genética , Herpesvirus Humano 1/imunologia , Herpesvirus Humano 1/isolamento & purificação , Humanos , Imunoglobulina G/sangue , Pessoa de Meia-Idade , Isquemia Miocárdica/cirurgia , Reação em Cadeia da Polimerase , Simplexvirus/genética , Simplexvirus/imunologia , Simplexvirus/isolamento & purificaçãoRESUMO
The primary and secondary structures of the pp65 phosphoprotein of human cytomegalovirus coded by the UL83 gene were studied by the methods of computer-aided analysis. An immunodominant protein fragment with 3 antigenic determinant was detected. The UL83 fragment coding the selected region was amplified and cloned in bacterial expressing vector. The recombinant protein was obtained and purified. On the basis of ELISA findings it was acknowledged as possible to use the pp65 recombinant protein jointly with pp150 and p52 in the diagnosis of antibodies specific to human cytomegalovirus.
Assuntos
Citomegalovirus/metabolismo , Fosfoproteínas/genética , Proteínas da Matriz Viral/genética , Sequência de Aminoácidos , Ensaio de Imunoadsorção Enzimática , Epitopos/química , Dados de Sequência Molecular , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Estrutura Secundária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Proteínas da Matriz Viral/química , Proteínas da Matriz Viral/metabolismoRESUMO
The process of the disease due to herpes simplex virus types 1 and 2 (HSV-1 and 2) was studied on white uninbred mice weighing 10 to 12 g. The animals were infected intracerebrally or intraperitoneally. Intraperitoneal contamination of the animals with MS strain of HSV-2 was used for the experimental model of the herpes simplex infection. The prophylactic antiherpes action of ultralow doses of the human gamma-interferon antibodies (ULD of anti-IFN-gamma) at a course of its intragastral administration was evaluated. The preparation was shown to have a significant (p < 0.05) protective effect in a dose of 10 LD50, evident from a 10-fold decrease of the HSV-2 accumulation in the brain, a lower percentage of the animal deaths and an increase of the average lifespan of the animals by 3.3 days. The study of the therapeutic action of ULD of anti-IFN-gamma at a course of its intragastral administration showed that the preparation had no significant positive effect on the disease process in the animals infected with HSV-2 in a dose of 10 LD50. However, a positive effect associated with delayed virus replication in the brain was observed in the study on the therapeutic effect of ULD of anti-IFN-gamma after its intragastral administration to the mice infected with a sublethal dose of the virus.
Assuntos
Anticorpos/administração & dosagem , Herpes Simples/tratamento farmacológico , Herpesvirus Humano 1 , Herpesvirus Humano 2 , Interferon gama , Replicação Viral/efeitos dos fármacos , Animais , Anticorpos/imunologia , Modelos Animais de Doenças , Feminino , Interferon gama/imunologia , Masculino , Camundongos , CoelhosRESUMO
The association between Cytomegalovirus (CMV) and Herpes simplex (HSV-1), on the one hand, and the coronary heart disease and chronic venous insufficiency (CVI), on the other hand, was studied; besides the infection rate by the above viruses in different age groups of donors was examined. The following methods--immune-enzyme analysis and detection of viral DNA by means of the polymerase chain reaction (PCR) as well as immune-fluorescence scanning of atherosclerotic plaques--were made use in the study. The pathogenesis of coronary heart disease was shown to be related with the viral contamination caused by CMV, while there was no such correlation between the former and the CVI patients. Finally, it was established that, with age, the frequency rate of seropositive reactions to CMV and HSV-1 was increasing in virtually healthy people.
Assuntos
Doença das Coronárias/microbiologia , Infecções por Citomegalovirus/complicações , Citomegalovirus/isolamento & purificação , Herpes Simples/sangue , Herpesvirus Humano 1/isolamento & purificação , Adulto , Doença das Coronárias/patologia , Citomegalovirus/genética , Citomegalovirus/imunologia , Herpes Simples/imunologia , Herpes Simples/virologia , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Two strains of cytomegalovirus were isolated from seropositive patients. The strains were identified and characterized by virological and immunological methods and may be used for research and practical studies.
Assuntos
Citomegalovirus/isolamento & purificação , Linhagem Celular , Citomegalovirus/classificação , Citomegalovirus/fisiologia , Infecções por Citomegalovirus/virologia , DNA Viral/análise , Eletroforese em Gel de Poliacrilamida , Humanos , Reação em Cadeia da Polimerase , Especificidade da Espécie , Proteínas Virais/análiseRESUMO
Data on the immunopathogenesis of Ebola fever in laboratory animals are presented and the efficacy of some methods of vaccine prophylaxis discussed. Antiviral immunity induced in guinea pigs by injection of inactivated viral agents did not protect them from infection, whereas injections of a nonlethal strain of the virus in ascending doses led to the formation of immunity preventing the development of disease upon inoculation with a lethal strain in high doses. The role of some viral peptides in the development of immune response is shown and variants of recombinant constructions for the prevention of Ebola fever are offered.
