Assuntos
RNA Polimerases Dirigidas por DNA/metabolismo , Regulação Viral da Expressão Gênica , Proteínas do Envelope Viral/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Recombinante , Vírus da Encefalite Equina Venezuelana/genética , Escherichia coli/genética , Humanos , Dados de Sequência Molecular , Plasmídeos , Proteínas Virais , alfa 1-Antitripsina/genética , beta-Galactosidase/genéticaRESUMO
Employment of radioimmunoassay led to the demonstration of serological crossing between tick-borne encephalitis (TBE) virus and Venezuelan equine encephalomyelitis (VEE) virus. Using hybridoma technology, three hybridomas were produced secreting monoclonal antibodies (MAb) cross-reacting with these two viruses. With MAb, the epitope of binding of these antibodies was shown to be located on protein E of TBE virus and protein E1 of VEE virus. Despite the low percentage (14%) of homology of amino acid sequences of these proteins, 12 areas with homology from 24% to 63% were demonstrated. Considering conservative replacements, homology of these areas was 53%-75%. The assumed existence of some of these areas in alpha-helical conformation may explain the observed immunological crossing.