[A test for sperm cell survival in peritoneal fluid]. / Test przezycia plemników w plynie otrzewnowym (Test P.P.O).

The role of the peritoneal fluid in the physiology of reproduction, as well as in the transportation and survival of gametes, is little known. The authors have examined interactions between spermatozoa and the peritoneal fluid, collected during laparoscopy in the, so-called, survival test, from 42 infertile couples. The studied survival of spermatozoa in the peritoneal fluid was relatively high--19% after 48 hours--longer than in Menezo B2 fluid. Values of the test have been indicated, especially in cases of endometriosis-caused and idiopathic infertility.

[Postcoital test evaluated in peritoneal fluid]. / Test po stosunku oceniany w plynie otrzewnowym.

The role of the peritoneal fluid in the physiology of reproduction, as well as in the transportation and survival of gametes, is little recognized. Taking this into consideration, the authors have examined the occurrence of spermatozoa in the peritoneal fluid, collected from patients during diagnostic laparoscopies, following intrauterine insemination with husband's sperm, in, so-called, Templeton's Test. In the group of patients with cervical factor six (6) mobile spermatozoa (85%) were observed, in the group with male factor -- three (3) (42%) and in the group with idiopathic infertility -- 1 (25%). A high utility of this test has been indicated, especially in infertility caused by the male factor and in endometriosis.

[Treatment of infertility in marriage using a method of transcervical gamete introduction into the fallopian tube under control of USG]. / Leczenie nieplodnosci malzenskiej metoda przezszyjkowego gamet do jajowodu pod kontrola USG.

GIFT under USG control (USGIFT) was performed in 18 females between may and december of 1992. The ovulation was stimulated by gonadotropin and Clomid. In result we obtained four pregnancies--three normal uterine pregnancies and one ectopic pregnancy. One of these pregnant women gave birth to a child in February 1993.

Effect of interaction between methotrexate and dihydrofolate reductase on DNA synthesis in L1210 cells in vitro.

L1210 leukaemia cells were preloaded with [(3)H methotrexate] (MTX) to saturate high-affinity intracellular sites, and were then incubated with [(3)H]MTX to determine the steady-state intracellular MTX concentrations at extracellular concentrations ranging from 10 µM to zero. In addition, incubations to generate incomplete saturation of high-affinity intracellular MTX-binding sites were also carried out. Following determination of the total intracellular MTX, cells were pulsed with deoxyuridine (UdR) and its incorporation into DNA examined to assess the role of exchangeable and bound intracellular MTX on DNA synthesis. Further, cell pellets were disrupted and dihydrofolate reductase (DHFR) activity determined under each experimental condition. Extracellular MTX concentrations in excess of 1 µM depressed UdR incorporation to <2% of control, but incorporation rapidly recovered to 62% of control at the point of MTX-DHFR equivalence, and exceeded control values when all high-affinity sites were not saturated. DHFR activity was undetectable at extracellular MTX concentrations >0.50 µM, and never exceeded 6.09% of control at the "equivalence point" where all high-affinity sites were saturated. When less than 10% of potential inhibitor sites were occupied, enzyme activity increased rapidly, but never reached control. However, 5% of the DHFR activity was sufficient to permit UdR incorporation to continue at 50% of control levels, and UdR incorporation returned to control levels at 20% of the DHFR activity. The relationship between cellular MTX content and DNA synthesis or DHFR activity is sigmoid, suggesting a reversible interaction between enzyme and inhibitor. This lends support to the notion that "free" intracellular MTX is necessary for a maximal antitumour effect, and may explain its role in "high-dose" MTX therapy in man.