The association of body composition assessment with hospital length of stay in off-pump coronary artery bypass patients.

Introduction: Bioelectrical impedance analysis is a widely available, non-invasive method for body composition assessment. Aim: To elucidate the perioperative body composition alterations and their prognostic utility for hospital length of stay (LOS) in low risk, off-pump coronary artery bypass (OPCAB) patients. Material and methods: Fifty patients undergoing elective OPCAB were included in the study. Body composition assessments were performed 1 day before the scheduled surgery and on the 6th postoperative day. Patients were grouped into < 9 days (n = 29, 58%) and ≥ 9 days (n = 21, 42%). Multivariate logistic regression analysis was performed to create a body composition-based screening panel for prolonged hospital stay. Results: No significant differences in anthropometric measurements, clinical characteristics or occurrence of postoperative complications were detected between the study groups. Patients with longer hospitalization had significantly higher content of fat mass (FM%) and fat mass index (FMI), and significantly lower content of fat free mass (FFM%) baseline parameters (p = 0.011, p = 0.04 and p = 0.012, respectively). High FM% values had 15-fold, low FFM% values had 13-fold and high FMI values had 7-fold higher risk of experiencing longer stay in the hospital (p = 0.001, p = 0.001 and p = 0.005, respectively). The combined panel of three variables (higher FM%, lower FFM% and higher FMI) had 16-fold higher risk of longer hospitalization (adjusted OR = 16.40; 95% CI: 3.52-76.34; p = 0.0004). Conclusions: Preoperative high FM and low FFM content are independent predictors of prolonged hospital length of stay in normal- and increased-BMI patients after OPCAB.

Retrospective Reports of Parental Feeding Practices and Current Eating Styles in Polish Adults.

The role of childhood food experiences (CFEs) in determining future eating behaviors remains unclear. The aim of the study was to examine the link between CFEs and selected eating styles (ESs), i.e., intuitive (IE), restrained (ResEat) and external (ExtEat) eating, among 708 Polish adults aged 18-65 (477 women and 231 men). CFEs were measured with the Adults' Memories of Feeding in Childhood questionnaire. Polish versions of the Intuitive Eating Scale-2 and Dutch Eating Behavior Questionnaire were used to assess ESs. Mann-Whitney U test was used to compare ESs scores between those with lower and higher CFEs. In the total sample, "Restrictions" and "Pressure and Food Reward" parental feeding practices favored lower IE, while "Healthy Eating Guidance" and "Monitoring" practices predisposed higher levels of IE in adulthood. "Restrictions" were found to correlate with greater chances of ResEat, whereas "Healthy Eating Guidance" was linked with lower probability of ResEat. "Pressure and Food Reward" and "Monitoring" were associated with higher score for ExtEat. "Restrictions", "Child Control", "Monitoring" and "Healthy Eating Guidance" practices were differently linked to ESs in women and men. The findings suggest that education programs for parents should focus on the long-term consequences of feeding practices.

Childhood Food Experiences and Selected Eating Styles as Determinants of Diet Quality in Adulthood-A Cross-Sectional Study.

Available studies suggest that childhood food experiences (CFE) may be linked with eating behaviors in adulthood, as well as eating style (ES); thus, both CFE and ES can determine dietary intake. Little is known about the role of both of these factors in explaining the diet quality (DQ) of adults. The aim was to investigate to what extent selected ESs, i.e., intuitive (IE), restrained (ResEat), and external (ExtEat) eating, and CFE related to parental feeding practices (PFPs) will predict the DQ of women and men. Data from 708 Polish adults (477 women and 231 men) aged 18-65 were collected via the Internet from October 2022 to January 2023. Mann-Whitney's U Test was used to compare ES and CFE levels among women and men, while DQ determinants were tested with the multiple linear regression (MLR). In the total study sample, "Healthy Eating Guidance" (CFE), "Child Control" (CFE), "Body-Food Choice Congruence" (IE), and ResEat favored higher DQ scores, while "Unconditional Permission to Eat" (IE), "Eating for Physical Rather Than Emotional Reasons" (IE), and ExtEat predisposed to lower DQ scores. After the MLR was conducted separately in women and men, differences were noted in the role of "Healthy Eating Guidance" (CFE), "Pressure and Food Reward" (CFE), "Unconditional Permission to Eat" (IE), "Eating for Physical Rather Than Emotional Reasons" (IE), ExtEat, and ResEat in predicting DQ indices. Our findings suggest that childhood food experiences and selected eating styles may differently determine the DQ of women and men. Future studies conducted within representative samples are needed to confirm these results.

A Comprehensive Investigation of the Mechanical and Tribological Properties of AZO Transparent Conducting Oxide Thin Films Deposited by Medium Frequency Magnetron Sputtering.

This paper presents a detailed analysis of aluminium-doped zinc oxide (AZO) thin films and considers them a promising alternative to indium tin oxide in transparent electrodes. The study focusses on critical properties of AZO, including optical, electrical, and mechanical properties, with potential applications in displays, photovoltaic cells, and protective coatings. The deposited AZO thin films are characterised by excellent optical and electrical parameters, with transparency in the visible light range exceeding 80% and resistivity of 10-3 Ω·cm, which gives a high value of figure of merit of 63. Structural analysis confirms the nanocrystalline nature of as-deposited AZO thin films, featuring hexagonal ZnO, orthorhombic Al2O3, and cubic Al2ZnO4 phases. The study includes nanoindentation measurements, which reveal exceptional hardness (11.4 GPa) and reduced elastic modulus (98 GPa), exceeding typical values reported in the literature, highlighting their protective potential. Abrasion tests have shown extraordinary scratch resistance due to the lack of impact on topography and surface roughness up to 10,000 cycles. This comprehensive study demonstrated that as-deposited AZO thin films are multifunctional materials with exceptional optical, electrical, and mechanical properties. The findings open up possibilities for a variety of applications, especially in protective coatings, where the combination of hardness, scratch resistance, and transparency is both rare and valuable.

Polish Adaptation and Validation of the Intuitive (IES-2) and Mindful (MES) Eating Scales-The Relationship of the Concepts with Healthy and Unhealthy Food Intake (a Cross-Sectional Study).

Intuitive (IE) and mindful (ME) eating share internally focused eating, yet previous studies have shown that these concepts are not strongly correlated, which suggests that they might be differently related to food intake. The study aimed to adapt the original Intuitive (IES-2) and Mindful (MES) Eating Scales to the Polish language, to test their psychometric parameters and, further, to examine associations of IE and ME with an intake of selected food groups, i.e., healthy foods (fresh and processed vegetables, fresh fruit) and unhealthy foods (sweets, salty snacks). A cross-sectional study was conducted in 2020 in a group of 1000 Polish adults (500 women and 500 men) aged 18-65 (mean age = 41.3 ± 13.6 years). The factor structure was assessed with exploratory (EFA) and confirmatory (CFA) factor analysis as well as structural equation modeling (SEM). Measurement invariance across gender was assessed with multiple-group analysis. Internal consistency and discriminant validity of the two scales was tested. Spearman's correlation coefficient was used to examine the correlation between IES-2 and MES subscales with food intake. A 4-factor, 16-item structure was confirmed for IES-2, while EFA and CFA revealed a 3-factor, 17-item structure of MES. Both scales demonstrated adequate internal consistency and discriminant validity. Full metric and partial scalar invariance were found for IES-2, while MES proved partial invariances. "Awareness" (MES) and "Body-Food Choice Congruence" (IES-2) positively correlated with intake of healthy foods and negatively with the intake of unhealthy ones. "Eating For Physical Rather Than Emotional Reasons" (IES-2) and "Act with awareness" (MES) favored lower intake of unhealthy foods, whereas "Unconditional Permission to Eat" and "Reliance on Hunger and Satiety Cues" (IES-2) showed an inverse relationship. A greater score in "Acceptance" (MES) was conducive to lower intake of all foods except sweets. The results confirmed that adapted versions of the IES-2 and MES are valid and reliable measures to assess IE and ME among Polish adults. Different IE and ME domains may similarly explain intake of healthy and unhealthy foods, yet within a single eating style, individual domains might have the opposite effect. Future studies should confirm our findings with the inclusion of mediating factors, such as other eating styles, childhood experiences, dieting, etc.

The Influence of Substrate Topography on the Plasma Jet Flow in the Substrate Boundary Layer under Suspension Plasma Spray Conditions: A Numerical Approach.

This study deals with the numerical analysis of the plasma jet behavior close to the substrate surface depending on its topography. It uses a 2D axisymmetric time-dependent CFD model solved with the Ansys Fluent 2020/R1 package. The model takes into consideration the nonlinear thermophysical properties and turbulent phenomena of the plasma jet as well as its interaction with the microtextured substrate. Representative substrate topographies were considered as a boundary condition in the numerical simulations. They correspond to the bond coats used in Thermal Barrier Coating technology, actually APS sprayed NiCrAlY coatings which were experimentally microtextured using various laser unit operational conditions resulting in different substrate topographies. The numerical calculations showed that the substrate topography, modified and controlled in this work by microtexturing, disturbs the homogeneity of the pressure field in the substrate boundary layer resulting in the periodical pressure fluctuation. It was also observed that the relative local pressure disturbance is more significant in the substrate outer regions than close to the centerline. Then, based on the results of numerical calculations, the potential movement of feedstock particles near to the substrate was discussed. It was concluded that the deposition of fine powders, characterized by a low Stokes number, will be influenced by the pressure field distribution near to the substrate and will take place mainly in the local high-pressure zones. Furthermore, the local swirl of plasma taking place in each fine microtexture, created here by laser ablation, privileges the deposition of such particles on the surface asperities. These observations show that the CFD code modeling opens the possibility of predicting the movement and deposition of particles during plasma spraying, which is essential for understanding coating deposition mechanisms in suspension plasma spray.

Waste management in the mining industry of metals ores, coal, oil and natural gas - A review.

Waste generated due to mining activity poses a serious issue due to the large amounts generated, even up to 65 billion tons per year, and is often associated with the risk posed by its storage and environmental management. This work aims to review waste management in the mining industry of metals ores, coal, oil and natural gas. It includes an analysis and discussion on the possibilities for reuse of certain types of wastes generated from mining activity, and discusses the benefits, disadvantages and the impact of waste management on the environment. The article presents current methods of waste management arising during the extraction and processing of raw materials and the threats resulting from its application. Furthermore, the potential methods of mining waste management are discussed through an in-depth characterization of the properties and composition of various types of rocks. The presented work addresses not only the issues of more sustainable management of waste from the mining industry, but also responds to the current efforts to implement the assumptions of a circular economy, which is aimed at closing the loop. The methods of recycling by-products and treating waste as a resource more and more often not only meet environmental expectations, but also become a legal requirement. In this respect, the presented work can serve as a valuable support in decision-making about waste management.

Superhydrophobic sponges based on green deep eutectic solvents for spill oil removal from water.

The paper described a new method for crude oil-water separation by means of superhydrophobic melamine sponges impregnated by deep eutectic solvents (MS-DES). Due to the numerous potential of two-component DES formation, simple and quick screening of 156 non-ionic deep eutectic solvents using COSMO-RS (Conductor-like Screening Model for Real Solvents) computational model was used. DES which were characterized by high solubility of hydrocarbons and the lowest water solubility were synthesized and embedded on melamine sponges. The new sponges were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and goniometer. Several parameters affecting the crude oil-water separation (i.e. type and amount of DES, density and porosity of sponges, water contact eagle) were thoroughly studied. In order to studies of MS-DES affinity to the selected groups of crude oil i.e. Saturated, Aromatic, Resins, Asphaltenes (SARA) the thin layer liquid chromatography-flame ionization detection (TLC-FID) was used. The obtained results indicate that the melamine sponges impregnated by DES composed of eucalyptol and menthol in 1:5 molar ratio have high real crude oil absorption capacity in the range of 96.1 - 132.2 g/g and slightly depends on crude oil compositions, superhydrophobic properties (water contact angle 152°), low density of 9.23 mg/cm3, high porosity of 99.39%, and excellent reusability which was almost not changing even after 80 cycles. The outcomes indicate that new MS-DES materials could be excellent alternatives as absorbents for the cleanup of crude oil-polluted water.

Polish Adaptation of the Dutch Eating Behaviour Questionnaire (DEBQ): The Role of Eating Style in Explaining Food Intake-A Cross-Sectional Study.

Knowledge of associations between emotional, external, and restrained eating with food choices is still limited due to the inconsistent results of the previous research. The aim of the study was to adopt the Dutch Eating Behavior Questionnaire (DEBQ) and then to examine the relationship between emotional, external, and restrained eating styles and dietary patterns distinguished on the basis of intake of fruit and vegetables (fresh and processed separately), fruit and/or vegetable unsweetened juices, sweets and salty snacks, and the adequacy of fruit and vegetable intake. The cross-sectional study was conducted in 2020, in a sample of 1000 Polish adults. The questionnaire consisted of the Dutch Eating Behavior Questionnaire, questions on selected food groups intake, and metrics. DEBQ structure was tested using both exploratory and confirmatory factor analysis (EFA, CFA) and structural equation modelling (SEM), while multi-group analysis was used to test measurement invariance. Logistic regression was applied to investigate the association between eating styles and dietary patterns, identified with the use of K-means cluster analysis. EFA, CFA and SEM revealed a three-factor, 29-item tool with satisfactory psychometric parameters. Restrained eating (ResEat) and external eating (ExtEat) were found to decrease chances of low intake of both favorable (fruit, vegetables, and unsweetened juices) and unfavorable (sweets and salty snacks) foods and increased the chances of their moderate intake. ResEat increased the probability of the high intake of favorable and moderate or high intake of unfavorable foods. ResEat and ExtEat were predisposed to adequate intake of fruit and vegetables while emotional eating had the opposite effect. Gender, education, and BMI were also found to determine food intake. Our results provide evidence that both eating styles and sociodemographic characteristics should be taken into account while explaining food intake as they may favor healthy and unhealthy eating in different ways.

Investigation of Mechanical and Magnetic Properties of Co-Based Amorphous Powders Obtained by Atomization.

Properties of Co-based alloys with high Glass Forming Ability (GFA) in the form of powder are still not widely known. However, powders of high GFA alloys are often used for the development of bulk metallic glasses by additive manufacturing. In this work Co47.6B21.9Fe20.4Si5.1Nb5% at. and Co42B26.5Fe20Ta5.5Si5Cu1% at. were developed by gas-atomization. Obtained powders in size 50-80 µm were annealed at Tg and Tx of each alloy. Then SEM observation, EDS analyses, differential thermal analysis, X-ray diffraction, nanoindentation, Mössbauer, and magnetic properties research was carried out for as-atomized and annealed states. The gas atomization method proved to be an efficient method for manufacturing Co-based metallic glasses. The obtained powder particles were spherical and chemically homogeneous. Annealing resulted in an increase of mechanical properties such as hardness and the elastic module of Co47.6B21.9Fe20.4Si5.1Nb5% at and Co42B26.5Fe20Ta5.5Si5Cu1%, which was caused by crystallization. The magnetic study shows that Co47.6B21.9Fe20.4Si5.1Nb5 and Co42B26.5Fe20Ta5.5Si5Cu1 are soft magnetic and semi-hard magnetic materials, respectively.

Does Examining the Childhood Food Experiences Help to Better Understand Food Choices in Adulthood?

Impact of parental feeding practices on children's eating behaviors is well-documented in the literature. Nevertheless, little is known about how many of these behaviors might persist into adulthood. There is a lack of a tool measuring childhood feeding experiences recollected by adults, while the Comprehensive Feeding Practices Questionnaire (CFPQ) is used to measure parental feeding practices applied towards children. The aim of the study was to adapt the CFPQ to measure adults' recollections of their childhood (5-10 years old) feeding experiences, to examine its discriminant validity and then to assess if these practices are related to adults' food choices. In 2020, the modified version of CFPQ (mCFPQ) and questions on current food consumption were administered in a group of 500 adults twice over a two-week interval. The analysis included 443 participants whose questionnaires were correctly completed in both stages of the study. The Q-sorting procedure was used to test for discriminant validity of the questionnaire, i.e., confirmatory and exploratory factor analysis (EFA), Cronbach's alpha, correlations coefficients, and the analysis of the differences between groups according to the intake of certain food products. Test-retest reliability was examined by calculating interclass correlation coefficients (ICC) for each obtained factor. As a result of EFA, five subscales were identified: "Restrictions", "Healthy Eating Guidance", "Pressure and Food Reward", "Monitoring", and "Child Control". Items from these subscales created a new tool-Adults' Memories of Feeding in Childhood (AMoFiC). Test for internal consistency, factor correlations, and discriminant validity proved satisfactory psychometric parameters of AMoFiC. "Pressure and Food Reward" and "Child Control" were associated with higher intake of sweets and salty snacks, whereas "Healthy Eating Guidance", "Monitoring", and "Restrictions" were associated with higher consumption of fresh fruits and vegetables. Despite the fact that the AMoFiC questionnaire requires further research, the findings of the study might be of practical use in counseling addressed to the parents.

3D X-ray Micro-CT Analysis of Rebar Corrosion in Reinforced Concrete Subjected to a Chloride-Induced Environment.

The paper presents experimental investigations of the concrete covers' protective ability to counteract rebar corrosion in reinforced concrete cubes. The concrete sample was subjected to a chloride-induced environment to get corroded and combined with an un-corroded sample. The chloride-accelerated technique can induce a high degree of corrosion within a controlled time. Moreover, detailed and thorough experimental measurements and analyses of reinforcement loss due to corrosion and its influence on concrete microstructure, were studied through 3D X-ray micro-computed tomography. The rebar outside the concrete was heavily corroded due to the chloride-accelerated test, whereas, only local surface corrosion products appeared inside the concrete. It turned out that the concrete cover showed protective ability to counteract the reinforcing-steel corrosion mechanism despite the accelerated corrosion environment. Moreover, the bond strength between the reinforcement rebar and concrete was not visibly affected since the failure force in the pull-out test and failure mechanisms, observed by 3D X-ray micro-CT, were similar for corroded and un-corroded samples. The failure occurred due to radial cracks with a maximum width equal to approximately 0.25 mm.

Relationship between Psychosocial Impairment, Food Choice Motives, and Orthorexic Behaviors among Polish Adults.

Orthorexic behaviors correlate not only with health motives when choosing food but may also coexist with psychosocial impairment. The aim of this study was to assess the motives of food choice and psychosocial impairment among adults with orthorexic behaviors through the use of ORTO-15 and ORTO-7. The data for the study were collected from a sample of 1007 Polish adults through a cross-sectional quantitative survey conducted in 2019. The respondents were asked to complete the ORTO-15 questionnaire, the Food Choice Questionnaire (FCQ), and the Clinical Impairment Assessment (CIA). Orthorexic behaviors were measured using both the 15-item and the shorter 7-item version of the ORTO questionnaire. To determine the factors coexisting with the orthorexic behaviors, linear regression models were developed. The scores of both ORTO-15 and ORTO-7 correlated positively with the global CIA scores and the scores of personal, cognitive, and social impairments, but compared to the ORTO-7 scores, the ORTO-15 scores showed weaker correlations with the global CIA score and individual CIA scales. Orthorexic behaviors measured with ORTO-15 correlated positively with such food choice motives as health, natural content, and weight control; whereas orthorexic behaviors measured with ORTO-7 showed positive bivariate correlations only with two food choice motives: health and weight control. In regression models, sensory appeal, age, and education lower than secondary were associated inversely with orthorexic behaviors measured by both the ORTO-15 and the ORTO-7. In conclusion, the obtained results confirm that orthorexic behaviors are associated with a higher score regarding health motivation and cause an increase in psychosocial impairment. In addition, orthorexic behaviors are associated with greater importance of body weight control, which confirms the relationship between orthorexic behaviors and other eating disorders (ED), such as anorexia nervosa (AN) and bulimia nervosa (BN). However, similar motives for food choice displayed by the groups with higher scores of the ORTO-15 and the ORTO-7 and strong correlation between results obtained from both tools confirmed the similarity between these two questionnaires, thus revealing the weak psychometric properties also of the shorter seven-item version of the ORTO. Future studies on food motives, psychosocial impairment, and orthorexic behaviors should consider using other tools for measuring orthorexic behaviors.

Does Eating Addiction Favor a More Varied Diet or Contribute to Obesity?-The Case of Polish Adults.

Rapidly increasing prevalence of overweight and obesity indicates a need to search for their main causes. Addictive-like eating and associated eating patterns might result in overconsumption, leading to weight gain. The aim of the study was to identify main determinants of food intake variety (FIV) within eating addiction (EA), other lifestyle components, and sociodemographic characteristics. The data for the study were collected from a sample of 898 Polish adults through a cross-sectional survey in 2019. The questionnaire used in a study included Food Intake Variety Questionnaire (FIVeQ), Eating Preoccupation Scale (EPS) and questions regarding lifestyle and socio-demographic factors. High eating addiction was found in more than half of people with obesity (54.2%). In the study sample physical activity at leisure time explained FIV in the greatest manner, then subsequently EPS factor: Eating to provide pleasure and mood improvement. In the group of people with obesity, the score of this EPS factor was the best predictor of FIV, in a way that its higher score was conducive to a greater variety of food intake. Socio-demographic characteristics differentiated FIV only within group with normal body weight (age) and with overweight (education). As conclusion, food intake variety (FIV) was associated with physical activity at leisure time, and then with EPS factor "Eating to provide pleasure and mood improvement", whereas socio-demographic characteristics were predictors of FIV only within groups identified by Body Mass Index (BMI). Nevertheless, our observations regarding Eating to provide pleasure and mood improvement factor and its associations with food intake variety indicate a need for further research in this area. Future studies should also use other tools to explicitly explain this correlation.

Silica Gel Impregnated by Deep Eutectic Solvents for Adsorptive Removal of BTEX from Gas Streams.

The paper presents the preparation of new adsorbents based on silica gel (SiO2) impregnated with deep eutectic solvents (DESs) to increase benzene, toluene, ethylbenzene, and p-xylene (BTEX) adsorption efficiency from gas streams. The DESs were synthesized by means of choline chloride, tetrapropylammonium bromide, levulinic acid, lactic acid, and phenol. The physico-chemical properties of new sorbent materials, including surface morphology and structures, as well as porosity, were studied by means of thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and Brunauer-Emmett-Teller analysis. The effect of DESs type, flow rate, and initial concentration of BTEX were also investigated followed by regeneration and reusability of adsorbents. The results indicate that SiO2 impregnated with tetrapropylammonium bromide and lactic acid in a 1:2 molar ratio have great potential for the removal of BTEX from gas streams. Its adsorption capacity was higher than the pure SiO2 and other developed SiO2-DES adsorbents. This result can be explained by the specific interaction between DESs and BTEX, i.e., hydrogen bonds interaction.

Iron(iii)-selective materials based on a catechol-bearing amide for optical sensing.

The synthesis and ion-binding properties of a new amide L derived from 3,4-dihydroxybenzoic acid are described. Due to the presence of a catechol unit, the compound interacts selectively with iron(iii) in organic solvent (dimethyl sulfoxide, DMSO) to produce a color change from pale yellow to green. The incorporation of the ligand L into polymeric matrices or its encapsulation into surfactant-based spheres enables analyte detection in aqueous solutions. The influence of the ligand environment (i.e. organic solvent, polymeric membrane or micelle) on the properties of the sensing materials is analyzed and the sensors are compared.

TECS: a toxin expression control strategy as a tool for optimization of inducible promoters.

BACKGROUND: Transcriptional control of gene expression is a widely utilized regulatory mechanism in synthetic biology, biotechnology and recombinant protein production. It is achieved by utilization of naturally occurring promoters responding to nutrients or chemicals. Despite their regulatory properties, these promoters often possess features which diminish their utility for biotechnology. High basal expression level and low induction ratio can be removed using genetic engineering techniques, although this process is often laborious and time-consuming. RESULTS: In order to facilitate optimization process for inducible promoters, we developed a simple method based on a conditional toxin expression which we abbreviate as toxin expression control strategy (TECS). In the presence of sucrose, SacB enzyme from Bacillus subtilis synthesizes levans which cause Eschericha coli cell lysis. However, in the absence of sucrose the enzyme does not affect the growth of the host. We utilized this feature to develop a two-step protocol allowing for efficient selection of inducible promoter variants. Using TECS we were able to modify the well-described pBAD promoter to decrease its leakage while maintaining high activity upon induction. Furthermore, we used the method to test transcriptional interference of lambda phage-derived sequence and optimize it for higher induction levels through random mutagenesis. CONCLUSIONS: We show that TECS is an efficient tool for optimization and development of inducible promoter systems in E. coli. Our strategy is very effective in the selection of promoter variants with improved properties. Its simplicity and short hands-on time make it an attractive method to optimize existing promoters and to construct novel, engineered genetic elements which improve properties of an inducible promoter system.