RESUMO
Geotrichum citri-aurantii is a postharvest phytopathogenic fungus of lemons. We studied the mode of action of antifungal metabolites from Bacillus sp. strain IBA 33 on arthroconidia of G. citri-aurantii. These metabolites are lipopeptides belonging to the iturin family. Membrane permeabilization of G. citri-aurantii was analyzed and mitochondrial respiratory rate was evaluated. Disturbance of the plasma membrane promotes the leakage of many cellular components into the surrounding media, and mitochondrial membrane disorganization promotes the inhibition of the respiratory rate. Our findings provide insights into the ability of lipopeptides to suppress plant fungal pathogens and their possible agronomical applications.
Assuntos
Bacillus/química , Citrus/microbiologia , Microbiologia de Alimentos , Geotrichum/efeitos dos fármacos , Antifúngicos/química , Membrana Celular/efeitos dos fármacos , Conservação de Alimentos , Bicamadas Lipídicas/química , Lipopeptídeos/química , Microscopia Eletrônica de Transmissão , Membranas Mitocondriais/efeitos dos fármacos , Permeabilidade , Potássio/química , Esporos Fúngicos/efeitos dos fármacosRESUMO
Alicyclobacillus acidoterrestris is considered to be one of the important target microorganisms in the quality control of acidic canned foods. There is an urgent need to develop a suitable method for inhibiting or controlling the germination and outgrowth of A.acidoterrestris in acidic drinks. The aim of this work was to evaluate the chemicals used in the lemon industry (sodium benzoate, potassium sorbate), and lemon essential oil as a natural compound, against a strain of A.acidoterrestris in MEB medium and in lemon juice concentrate. The results pointed out that sodium benzoate (500-1000-2000 ppm) and lemon essential oil (0.08- 0.12- 0.16%) completely inhibited the germination of A. acidoterrestris spores in MEB medium and LJC for 11 days. Potassium sorbate (600-1200 ppm) was more effective to inhibit the growth of the microbial target in lemon juice than in MEB medium. The effect of sodium benzoate, potassium sorbate and essential oil was sporostatic in MEB and LJC as they did not affect spore viability.
Assuntos
Alicyclobacillus/efeitos dos fármacos , Alicyclobacillus/fisiologia , Antibacterianos/farmacologia , Citrus/química , Viabilidade Microbiana/efeitos dos fármacos , Óleos Voláteis/farmacologia , Antibacterianos/isolamento & purificação , Meios de Cultura/química , Óleos Voláteis/isolamento & purificação , Benzoato de Sódio/farmacologia , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/fisiologiaRESUMO
Alicyclobacillus acidoterrestris is considered to be one of the important target microorganisms in the quality control of acidic canned foods. There is an urgent need to develop a suitable method for inhibiting or controlling the germination and outgrowth of A.acidoterrestris in acidic drinks. The aim of this work was to evaluate the chemicals used in the lemon industry (sodium benzoate, potassium sorbate), and lemon essential oil as a natural compound, against a strain of A.acidoterrestris in MEB medium and in lemon juice concentrate. The results pointed out that sodium benzoate (500-1000-2000 ppm) and lemon essential oil (0.08-0.12-0.16%) completely inhibited the germination of A. acidoterrestris spores in MEB medium and LJC for 11 days. Potassium sorbate (600-1200 ppm) was more effective to inhibit the growth of the microbial target in lemon juice than in MEB medium. The effect of sodium benzoate, potassium sorbate and essential oil was sporostatic in MEB and LJC as they did not affect spore viability.
Assuntos
Alicyclobacillus/efeitos dos fármacos , Alicyclobacillus/fisiologia , Antibacterianos/farmacologia , Citrus/química , Viabilidade Microbiana/efeitos dos fármacos , Óleos Voláteis/farmacologia , Antibacterianos/isolamento & purificação , Meios de Cultura/química , Óleos Voláteis/isolamento & purificação , Benzoato de Sódio/farmacologia , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/fisiologiaAssuntos
Doença de Fabry/diagnóstico , Doença de Fabry/terapia , Adulto , Fatores Etários , Brasil , Estudos de Coortes , Doença de Fabry/etiologia , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Guias de Prática Clínica como Assunto , Fatores Sexuais , Resultado do Tratamento , alfa-Galactosidase/genética , alfa-Galactosidase/metabolismoRESUMO
Numerous species of filamentous fungi were isolated from lemon on different plantations in the province of Tucuman, Argentina. The techniques suggested by the Subcommittee of Antifungal Susceptibility of the National Committee for Clinical Laboratory Standards, (USA) were adapted. The effect of three different concentrations of the fungicides imazalil, guazatine, SOPP and thiabendazole on the fungi Fusarium oxysporum, Fusarium moniliforme, Aspergillus niger, Aspergillus flavus, Aspergillus clavatus, Geotrichum candidum, Rhizopus sp, Penicillium sp, Penicillium digitatum and Mucor sp were studied. All the tested strains were resistant to thiabendazole. We assayed a mixture of SOPP (5%), guazatine (350 ppm) and imazalil (100 ppm), which showed a synergic effect on Rhizopus sp. Mucor sp was the only fungus resistant to the four fungicides tested as well as to the above mentioned mixture.
