RESUMO
Outer sheath antigen was prepared from Leptospira interrogans serovars pomona, sejroe and hardjo by treating the organisms with 1.0M NaC1 followed by 0.04% sodium dodecyl sulfate (SDS). Sodium dodecyl sulfate was removed from the SDS-protein complexes by the extraction of dodecyl sulfate anions as ion pairs with triethylammonium cations into an organic solvent. The outer sheath antigen was recovered from the organic solvent as a precipitate and used as the source of leptospiral enzyme-linked immunosorbent assay (ELISA) antigen. Utilizing this antigen, ELISA was adapted to detect bovine serum antibody to L. interrogans serovars pomona, sejroe and hardjo. The specificity of this assay in 344 bovine sera, which were negative in the microscopic agglutination test (MAT) for seven serovars, was 99.4%. In sera from 37 and 87 cattle which revealed MAT titers greater than or equal to 1:50 for L. interrogans serovars pomona and sejroe, the relative sensitivity of the test was 100%. The ELISA also showed a considerable degree of low level cross-reactivity with other serovars. Sixty-six (75.9%) out of 87 bovine sera which were MAT-positive (MAT titer of greater than or equal to 1:50) with serovars sejroe and hardjo only were ELISA positive with heterologous pomona antigen; 16 (43.2%) and six 16.2%) out of 37 bovine sera which were MAT positive MAT titer of greater than or equal to 1:50) with serovar pomona only were ELISA positive with heterologous sejroe and hardjo ELISA antigen respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Anticorpos Antibacterianos/análise , Doenças dos Bovinos/diagnóstico , Bovinos/imunologia , Leptospira interrogans/imunologia , Leptospirose/veterinária , Animais , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/microbiologia , Ensaio de Imunoadsorção Enzimática , Leptospirose/diagnóstico , Leptospirose/imunologiaAssuntos
Animais Domésticos , Encéfalo/microbiologia , Listeria monocytogenes/isolamento & purificação , Listeriose/veterinária , Animais , Bovinos , Cães , Doenças dos Cavalos/microbiologia , Cavalos , Listeriose/microbiologia , Ontário , Ovinos , Doenças dos Ovinos/microbiologia , Suínos , Doenças dos Suínos/microbiologiaRESUMO
The results of a survey which included the testing of 21,275 blood samples collected at various slaughter houses are described.Sixty-three herds had a single reactor with a titre of 1:100 or higher to a tube or plate agglutination test. Investigations in forty-five of these herds failed to detect the presence of brucellosis in the remaining mature swine.The prompt slaughter of the reactors may have eliminated possible sources of infection.