Tungsten-induced protein aggregation: solution behavior.

Tungsten has been associated with protein aggregation in prefilled syringes (PFSs). This study probed the relationship between PFSs, tungsten, visible particles, and protein aggregates. Experiments were carried out spiking solutions of two different model proteins with tungsten species obtained from the extraction of tungsten pins typically used in syringe manufacturing processes. These results were compared to those obtained with various soluble tungsten species from commercial sources. Although visible protein particles and aggregates were induced by tungsten from both sources, the extract from tungsten pins was more effective at inducing the formation of the soluble protein aggregates than the tungsten from other sources. Furthermore, our studies showed that the effect of tungsten on protein aggregation is dependent on the pH of the buffer used, the tungsten species, and the tungsten concentration present. The lower pH and increased tungsten concentration induced more protein aggregation. The protein molecules in the tungsten-induced aggregates had mostly nativelike structure, and aggregation was at least partly reversible. The aggregation was dependent on tungsten and protein concentration, and the ratio of these two and appears to arise through electrostatic interaction between protein and tungsten molecules. The level of tungsten required from the various sources was different, but in all cases it was at least an order of magnitude greater than the typical soluble tungsten levels measured in commercial PFS.

Hypnotic conflict: a brief report.

Two studies investigated management of conflict in hypnosis by subtly increasing the brightness of a visual stimulus during a suggestion for hypnotic blindness to the stimulus. In Study 1, 23 high hypnotizable participants were administered a hypnotic suggestion for blindness to a projected light. For half the participants, the brightness of the light was intensified during the suggestion. Behavioral ratings and online analog-dial measurement indicated that participants reported decreased hypnotic blindness during the increased conflict condition. In Study 2, 20 participants were administered the nonexperimental procedure to investigate the impact of demand characteristics in this paradigm. Parallel findings in Studies 1 and 2 indicated that demand characteristics may explain the response to hypnotic conflict. Limitations in applying the nonexperimental procedure to this paradigm and the need for further investigation are discussed.

Reality monitoring in hypnosis: a real-simulating analysis.

The extent to which hypnotic suggestions are perceived as real is central to understanding hypnotic response. This study indexed the reality attributed to hypnotic suggestion through subtle projection of a visual image during simultaneous suggestion for a visual hallucination that resembled the projected image. Twenty real hypnotized and 20 simulating nonhypnotized participants were administered a hypnotic induction and given a suggestion to hallucinate a shape, and then the projected image was introduced. Following the hypnosis session, an Experiential Analysis Technique was employed to index experiential responses. Real, but not simulating, participants made comparable reality ratings when the projected image was absent and present. Reals, but not simulators, also reported more effort in maintaining belief in the suggestion when the projection was absent. These findings suggest that the reality attributed to a hypnotic suggestion cannot be attributed to demand characteristics.

Reality monitoring in hypnosis: a pilot investigation.

In a pilot investigation of reality monitoring in hypnosis, 10 high and 10 low hypnotizable participants were administered a hypnotic suggestion to hallucinate a visual shape on a wall. For half the participants, an image was subtly projected onto the wall at the commencement of the suggestion and then subsequently removed. For the remaining participants, the projected image was initially absent and subsequently projected. Participants completed ratings of belief in the suggestion during hypnosis and also provided subjective reports of the suggestion during a subsequent Experiential Analysis Technique session. High hypnotizable participants who had the projected image introduced at the end of the suggestion provided comparable belief ratings when the image was present and absent. In contrast, highs who had the projected image presented first reported less belief when the image was absent than when it was present. Low hypnotizable participants rated the hallucination more strongly when the image was projected than when it was not projected. These pilot data are discussed in terms of developing a paradigm to objectively index the perceived reality of hypnotically suggested experiences.

SEM studies of the morphology and chemistry of polar ice.

Determining the microphysical location of impurities in natural ice from the polar regions is necessary for understanding the physical properties of ice and for assuring the integrity of ice core records. SEM, using a cold stage and X-ray microanalytical techniques, has proved to be the most powerful method so far for undertaking such work. Methods are adapted from those used to study frozen hydrated biological material. Sublimation within the cryo-chamber is often needed in order to concentrate impurities onto a plane, but this can lead to artifacts that must be recognized. Over 100 samples from different depths and sites in Greenland and Antarctica have been examined. Typical physical features, including air bubbles, clathrate hydrates of air, and dust particles are identified. The dust is found preferentially at grain boundaries in some samples; by pinning the boundaries, it can slow grain growth. Of the soluble material, chloride seems to be found most frequently in the ice lattice. Other impurities are found at grain boundaries, and only when the bulk concentration exceeds a threshold, at triple junctions. These findings give new insights into processes determining the physical properties of ice samples and of ice sheets, and new impetus for theoretical studies of the energetics that lead to this distribution.

Seeing is believing: the reality of hypnotic hallucinations.

Two experiments investigated the reality attributed to hypnotic suggestion through subtle projection of a visual image during simultaneous suggestion for a visual hallucination that resembled the projected image. In Experiment 1, high and low hypnotizable participants were administered either a hypnotic induction or wake instructions, given a suggestion to hallucinate a shape, and then the projected image was subsequently introduced. Although highs in both conditions rated the projected image more vividly than lows, highs in the hypnosis (but not wake) condition made comparable reality ratings when the projected image was absent and present. In Experiment 2, high hypnotizable participants were administered a suggestion to see a shape on a wall. For half the participants the suggested image was projected on the wall and then removed, and for half the projection was initially absent and then introduced. Participants who had the projection absent and then present reported comparable reality and vividness ratings when the projection was absent and present. These findings indicate that elevated hypnotizability and hypnosis are associated with attributions of external reality to suggested experiences.

Hypnotically induced emotional numbing: a real-simulating analysis.

This study compared 20 real, hypnotized and 20 simulating, unhypnotized participants who were administered a hypnotic induction and then presented with emotionally distressing and neutral visual images. Half were administered a hypnotic suggestion for emotional numbing. Reals and simulators who received the emotional numbing suggestion reported comparably less responsivity to distressing stimuli than others. Whereas emotionally numb reals displayed little change in electromyographic (EMG) activity during the distressing stimuli, simulators displayed marked reduction in EMG activity. Reals (not simulators) displayed a dissociation between their affective response and awareness of the negative content of the stimuli following the numbing suggestion.