[Metagenomic Analysis of Microbial Communities of the Sediments of the Kara Sea Shelf and the Yenisei Bay].

Microbial diversity in the sediments of the Kara Sea shelf and the southern Yenisei Bay, differing in pore water mineralization, was studied using massive parallel pyrosequencing according to the 454 (Roche) technology. Members of the same phyla (Cyanobacteria, Verrucomicrobia, Actinobacteria, Proteobacteria, and Bacteroidetes) predominated in bacterial communities of the sediments, while their ratio and taxonomic composition varied within the phyla and depended on pore water mineralization. Increasing salinity gradient was found to coincide with increased share of the γ-Proteobacteria and decreased abundance of α- and ß-Proteo- bacteria, as well as of the phyla Verrucomicrobia, Chloroflexi, Chlorobi, and Acidobacteria. Archaeal diversity was lower, with Thaumarchaeota predominant in the sediments with high and low mineralization, while Crenarchaeota predominated in moderately mineralized sediments. Microbial communities of the Kara Sea shelf and Yenisei Gulf sediments were found to contain the organisms capable of utilization of a broad spectrum of carbon sources, including gaseous and petroleum hydrocarbons.

[Possibilities of using laser doppler flowmetry in evaluating periodontal tissue status]. / Vozmozhnosti ispol'zovanie metoda lazernoi dopplerovskoi floumetrii (LDF) v otsenke sostoianiia tkanei parodonta.

Laser Doppler flowmetry (LDF) was used for evaluation of microcirculatory disorders in periodontal tissue of children in health and disease. Gingival capillary bloodflow was examined in children and adults with intact periodontium and orthognathic occlusion, chronic generalized catarrhal gingivitis and local periodontitis (with evaluation of the efficiency of laser therapy), in children with various maxillodental abnormalities, and in adults with partial and complete adentia. LDF findings were processed using mathematical analysis, and criteria for evaluation of capillary bloodflow in children of different age groups were defined. LDF detects microcirculatory disorders in periodontal tissues and differentiates between them, detects subjects at a high risk of periodontal inflammations, helps monitor the treatment course and evaluate the disease severity.