[Elaboration of the in vitro model system to study the interaction of phytopathogenic mollicutes with plant cells].

The model system based on the sugar beet calluses infected by mycoplasms (mollicutes) was elaborated, and changes in the callus cells morphology under the effect of these microorganisms were also studied. The calluses of sugar beet 3K51 cultivated on the Gamborg medium were infected by phytopathogenic mollicute Acholaplasma laidlawii var. granulum str.118. Under the effect of mollicute infection one could observe changes in the cell morphology of sugar beet calluses: the plant cells were transformed from round to lengthened, the intensity of polyploids forming was increased, their grouping and their total destruction were observed. Data of electron microscopy confirm the presence of the mollicute in the sugar beet calluses: acholeplasma cells were localized between and within undifferentiated plant cells.

[Electron-microscopic study of potato aucuba mosaic virus].

The results of electron-microscopic investigation of the peculiarities of virus interrelations with cells of mesophyl of the potato leaves, which were infected with potato aucuba mosaic virus (PAMV) are presented. Aggregates of the virus particles have been revealed which are localized in cytoplasm between the cellular organoids and just near the cellular membrane. The significant destructural changes of the structure of chloroplasts and formations of virus-induced inclusions in them are observed. The cells contain cytoplasmic inclusions--round and oval electron-dence formations which are surrounded with a friable membrane. They occupy the large zones of cytoplasm, that is a typical sign of PAMV infection.

[Purification of viral drugs for development of diagnostic aids of Teschen disease]. / Ochyshchennia virusnykh preparativ dlia rozrobky diahnostykumiv khvoroby Teshena.

Optimal methods of purification and concentration of teschevirus strain of the pigs of the first serotype "Chernigivsky-2372" have been chosen for development of the set of Teschen pig discase diagnosticums. It has been established that differential centrifugation with following ultracentrifugation in the gradient of saccharose density permits to deprive viruses from host cell proteins, empty virus capsides and defect virus particles. That is the method fit for purification of virus preparations for the solid-phase immunoenzyme analysis.