RESUMO
BACKGROUND: Preeclampsia is one of the three leading causes of worldwide maternal mortality. Oxidative stress-mediated endothelial damage is expected to be an ultimate common mechanism in the pathophysiology of preeclampsia. The role of bioamines is also well-established in the induction of preeclampsia. This project is aimed to understand the factors which may affect the induction, progression, and aggravation of preeclampsia and oxidative stress during pregnancy. This study will explore the methylation pattern of the Catechol-O-methyltransferase gene to determine its role in the pathogenesis of preeclampsia, association of Val158Met polymorphism with a wide range of oxidative stress biomarkers, major antioxidants vitamins, and blood pressure regulating amines in preeclamptic Pakistani women. METHODS AND ANALYSIS: In this prospective case-control study, 85 preeclamptic and 85 normotensive pregnant women will be recruited in their third trimesters. DNA will be extracted from peripheral blood and Val158Met polymorphism in the Catechol-O-methyltransferase gene will be examined on PCR amplified product digested with Hin1II (NlaIII) restriction enzyme, further validated by Sanger sequencing. Methylation-sensitive PCR will also be performed. Oxidative stress biomarkers, antioxidant vitamins, bioamines, and catechol-O-methyltransferase levels will be measured by ELISA. The data will be used to correlate maternal and fetal outcomes in both groups. DISCUSSION: This study will help to identify and understand the multifactorial path and cause-effect relationship of gene polymorphism, oxidative stress biomarkers, major antioxidants vitamins, and blood pressure regulating amines in the pathogenesis and aggravation of preeclampsia in the Pakistani population. The outcome of this project will be particularly helpful in reducing the incidence of preeclampsia and further improving its management.
Assuntos
Biomarcadores , Catecol O-Metiltransferase , Estresse Oxidativo , Pré-Eclâmpsia , Adulto , Feminino , Humanos , Gravidez , Antioxidantes/metabolismo , Biomarcadores/sangue , Estudos de Casos e Controles , Catecol O-Metiltransferase/genética , Predisposição Genética para Doença , Estresse Oxidativo/genética , Paquistão , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Pré-Eclâmpsia/genética , Estudos Prospectivos , Adulto JovemRESUMO
The care provided to persons who experience sexual violence is complex and needs to be individualized and account for the person's expressed needs. Nurses have the potential to facilitate the recovery from such traumatic events. Utilizing Watson's theory of human caring science, nursing care practices provide an influence through their utilization of artistry with caring-healing processes and attending to the healing environment for people. The authors in this article present a case study enlightening the applicability, implementation, and outcomes of caring for patients with this experience sensitively and holistically.