Hydrogen sulfide-mitigated salinity stress impact in sunflower seedlings was associated with improved photosynthesis performance and osmoregulation.

BACKGROUND: Salinity is one major abiotic stress affecting photosynthesis, plant growth, and development, resulting in low-input crops. Although photosynthesis underlies the substantial productivity and biomass storage of crop yield, the response of the sunflower photosynthetic machinery to salinity imposition and how H2S mitigates the salinity-induced photosynthetic injury remains largely unclear. Seed priming with 0.5 mM NaHS, as a donor of H2S, was adopted to analyze this issue under NaCl stress. Primed and nonprime seeds were established in nonsaline soil irrigated with tape water for 14 d, and then the seedlings were exposed to 150 mM NaCl for 7 d under controlled growth conditions. RESULTS: Salinity stress significantly harmed plant growth, photosynthetic parameters, the structural integrity of chloroplasts, and mesophyll cells. H2S priming improved the growth parameters, relative water content, stomatal density and aperture, photosynthetic pigments, photochemical efficiency of PSII, photosynthetic performance, soluble sugar as well as soluble protein contents while reducing proline and ABA under salinity. H2S also boosted the transcriptional level of ribulose 1,5-bisphosphate carboxylase small subunit gene (HaRBCS). Further, the transmission electron microscope showed that under H2S priming and salinity stress, mesophyll cells maintained their cell membrane integrity and integrated chloroplasts with well-developed thylakoid membranes. CONCLUSION: The results underscore the importance of H2S priming in maintaining photochemical efficiency, Rubisco activity, and preserving the chloroplast structure which participates in salinity stress adaptation, and possibly sunflower productivity under salinity imposition. This underpins retaining and minimizing the injury to the photosynthetic machinery to be a crucial trait in response of sunflower to salinity stress.

Sorghum under saline conditions: responses, tolerance mechanisms, and management strategies.

MAIN CONCLUSION: An overview is presented of recent advances in our knowledge of responses and mechanisms rendering adaptation to saline conditions in sorghum. Different strategies deployed to enhance salinity stress tolerance in sorghum are also pointed out. Salinity stress is a growing problem worldwide. Sorghum is the fifth key crop among cereals. Understanding responses and tolerance strategies in sorghum would be therefore helpful effort for providing biomarkers for designing greatest salinity-tolerant sorghum genotypes. When sorghum exposed to salinity, salinity-tolerant genotypes most probably reprogram their gene expression to activate adaptive biochemical and physiological responses for survival. The review thus discusses the possible physiological and biochemical responses that confer salinity tolerance to sorghum under saline conditions. Although it is not characterized in sorghum, salinity perceiving and transmitting signals to downstream responses via signaling transduction pathways most likely are essential strategy for sorghum adaptation to salinity stress. Sorghum has also shown to withstand moderate saline environments and retain the germination, growth, and photosynthetic activities. Salinity-tolerant sorghum genotypes show the ability to exclude excessive Na+ from reaching shoots and induce ion homeostasis. Osmotic homeostasis and ROS detoxification are also evident as salinity tolerance strategies in sorghum. These above mechanisms lead to re-establishment of cellular ionic, osmotic, and redox homeostasis as well as photosynthesis efficiency. It is noteworthy that these mechanisms act individually or co-operatively to minimize the salinity hazards and enhance acclimation in sorghum. We conclude, however, that although these responses contribute to sorghum tolerance to salinity stress, they seem to be not adequate at higher concentrations of salinity, which agrees with sorghum ranking as moderately salinity-tolerant crop. Also, some of these tolerance strategies reported in other crops are not well studied and documented in sorghum, but most probably have roles in sorghum. Further improvement in sorghum salinity tolerance using different approaches is definitely necessary to meet the requirements of its harsh production environments, and therefore, these approaches are addressed.