RESUMO
We have previously reported an identification of a novel human cellular factor, D40. Here, we report the chromosomal localization of the gene that encodes D40. Fluorescent in situ hybridization (FISH) was performed to determine the chromosomal region that D40 gene resides. The chromosomes that derived from normal adult male lymphocytes were hybridized with a mixture of cDNA probes that cover the entire coding region of D40. D40 gene mapped to the long arm of chromosome 15q14-15.
Assuntos
Proteínas de Transporte/genética , Cromossomos Humanos Par 15 , Adulto , Mapeamento Cromossômico , DNA Complementar , Humanos , Hibridização in Situ Fluorescente , Linfócitos/metabolismo , Masculino , Proteínas Associadas aos MicrotúbulosRESUMO
Previously, we have reported a novel protein, D40, that specifically binds to a nuclear factor GCF by two-hybrid system. Northern analysis on tumor cell lines revealed that mRNA of D40 is about 5 kb. To get cDNA clones that cover the total coding region of D40 protein, approximately 5 x 10(5) clones of a human cDNA library were screened, and then several positive clones were obtained. RACE was performed to get the 5' end of the cDNA. DNA sequencing revealed an open reading frame that encodes a protein with 887 amino acids. in vitro transcription and translation analysis of D40 revealed that the molecular weight of the protein is 110 to approximately 130 kda.
