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1.
J Med Case Rep ; 8: 248, 2014 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-25008098

RESUMO

INTRODUCTION: In general, enamel pearls are found in maxillary molars as a small globule of enamel. However, this case report describes an enamel pearl with a prolate spheroid shape which is 1.8mm wide and 8mm long. The different type of enamel pearl found in my clinic has significantly improved our understanding of enamel pearl etiology and pathophysiology. CASE PRESENTATION: A 42-year-old Han Chinese woman with severe toothache received treatment in my Department of Endodontics. She had no significant past medical history. A dental examination revealed extensive distal decay in her left mandibular first molar, tenderness to percussion and palpation of the periradicular zone, and found a deep periodontal pocket on the buccal lateral. Vitality testing was negative. Periapical radiographic images revealed radiolucency around the mesial apex. Cone beam computed tomography detected an opaque enamel pearl in the furcation area with a prolate spheroid shape of 1.8mm wide and 8mm long. CONCLUSION: The enamel pearl described in this case report is like a very long dental root. Cone beam computed tomography may be used for evaluating enamel pearls.


Assuntos
Esmalte Dentário/anormalidades , Dente Molar/anormalidades , Raiz Dentária/anormalidades , Adulto , Tomografia Computadorizada de Feixe Cônico , Esmalte Dentário/diagnóstico por imagem , Diagnóstico Diferencial , Feminino , Humanos , Dente Molar/diagnóstico por imagem , Dente Molar/cirurgia , Tratamento do Canal Radicular , Raiz Dentária/diagnóstico por imagem , Raiz Dentária/cirurgia
2.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 46(12): 759-61, 2011 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-22333360

RESUMO

OBJECTIVE: To investigate the effect of implant superstructure with platform switching to the osseointegration of implant-bone interface in immediate loading. METHODS: The bilateral mandiblular fourth premolars of 5 beagle dogs were extracted, and 3 months later, 10 implants were implanted and the abutments were accessed immediately to form immediate loading. Using self-control, the abutment with platform switching was used in the experimental side, and the traditional abutment used in the control side. The experimental animals were sacrificed after 3 months, and non-decalcified implant-bone sections were made. RESULTS: A favorable osseointegration of implant-bone interface in 4 animals (8 implants) was observed except for one failed case. A large number of osteoblasts and different mineralized bone were observed. In experimental side, the bone and implant-neck were nearly in the same level, but the bone around the implant-neck was significantly absorbed in control side. CONCLUSIONS: Using different superstructure in immediate loading could affect the osseointegration of implant-neck. The platform switching technology is conducive to the keeping of implant-neck bone.


Assuntos
Implantes Dentários , Osseointegração , Animais , Dente Pré-Molar , Osso e Ossos , Cães , Mandíbula
3.
Int Arch Allergy Immunol ; 139(1): 25-30, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16272823

RESUMO

BACKGROUND: Interleukin (IL)-13 has come to be appreciated as a molecule critically involved in allergic inflammatory responses. Recent studies revealed that a common variant in the coding region of the IL13 gene, Arg110Gln, has been implicated in the development of asthma and atopy. METHODS: To assess whether the IL13 variant Arg110Gln is associated with cedar pollinosis, one of the most common atopic diseases in the Japanese population, we examined the Arg110Gln variant using PCR-RFLP to compare the genotype and allele frequencies between 95 patients with cedar pollinosis and 95 healthy control subjects. Relationships between the Arg110Gln variant and the pollinosis-related traits, e.g. rhinitis severity, eosinophil counts in nasal secretion and serum total and allergen-specific IgE levels, were also investigated. RESULTS: The frequencies of the minor allele Gln110 were 25.8% in patients with cedar pollinosis and 30.9% in healthy control subjects (p > 0.05). There was also no significant difference in the genotype frequencies between cases and controls (p > 0.05). In addition, we found no significant association of the Arg110Gln variant with any of the pollinosis-related phenotypes (p > 0.05). CONCLUSIONS: Our data suggest lack of evidence for identifying the variant Arg110Glnat the IL13 locus as a genetic risk factor involved in the development of Japanese cedar pollinosis.


Assuntos
Cryptomeria/efeitos adversos , Predisposição Genética para Doença , Variação Genética , Interleucina-13/genética , Rinite Alérgica Sazonal/etiologia , Arginina , Genótipo , Ácido Glutâmico , Humanos , Japão , Pólen/efeitos adversos , Rinite Alérgica Sazonal/genética
5.
Hum Genet ; 117(1): 16-26, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15806396

RESUMO

Asthma is a phenotypically heterogeneous disorder with many etiologic factors and clinical characteristics. T-bet, a Th1-specific transcription factor of T-box family, has been found to control interferon-gamma (IFN-gamma) expression in T cells. Mice lacking the T-bet gene (tbx21) demonstrate multiple physiological and inflammatory features reminiscent of human asthma. In order to examine whether polymorphisms in the candidate gene, TBX21, located on chromosome 17q21.32, are related to the risk of human asthma phenotypes, we have searched for genetic variations in the human TBX21 gene and identified 24 single nucleotide polymorphisms (SNPs), including five novel SNPs, by direct sequencing in Japanese subjects. Among asthma phenotypes, a promoter -1993T-->C SNP, which is in linkage disequilibrium with a synonymous coding 390A-->G SNP in exon 1, is significantly associated with a risk of aspirin-induced asthma (AIA; P = 0.004, P(c) = 0.016). This association has also been confirmed in additional independent samples of asthma with nasal polyposis (P = 0.008), regardless of aspirin hypersensitivity. Furthermore, our data indicate that the -1993T-->C substitution increases the affinity of a particular nuclear protein to the binding site of TBX21 covering the -1993 position, resulting in increased transcriptional activity of the TBX21 gene. Thus, in addition to the antigen-driven excess Th2 response, increased T-bet (and subsequent IFN-gamma) production in human airways of individuals with the -1993T-->C polymorphism could contribute to the development of certain asthma-related phenotypes, such as AIA.


Assuntos
Anti-Inflamatórios não Esteroides/efeitos adversos , Aspirina/efeitos adversos , Asma/induzido quimicamente , Asma/genética , Polimorfismo de Nucleotídeo Único , Proteínas com Domínio T/genética , Adolescente , Anti-Inflamatórios não Esteroides/imunologia , Anti-Inflamatórios não Esteroides/uso terapêutico , Aspirina/imunologia , Aspirina/uso terapêutico , Sítios de Ligação , Criança , Pré-Escolar , Cromossomos Humanos Par 17 , Hipersensibilidade a Drogas/genética , Feminino , Predisposição Genética para Doença , Humanos , Interferon gama/imunologia , Japão , Masculino , Fenótipo , Regiões Promotoras Genéticas/genética , Fatores de Risco , Proteínas com Domínio T/imunologia , Células Th2/imunologia , Fatores de Transcrição/imunologia , Transcrição Gênica
6.
Hum Genet ; 115(4): 295-301, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15278436

RESUMO

Bronchial asthma (BA) is a common chronic inflammatory disease characterized by hyperresponsive airways, excess mucus production, eosinophil activation, and the production of IgE. The complement system plays an immunoregulatory role at the interface of innate and acquired immunities. Recent studies have provided evidence that C3, C3a receptor, and C5 are linked to airway hyperresponsiveness. To determine whether genetic variations in the genes of the complement system affect susceptibility to BA, we screened single nucleotide polymorphisms (SNPs) in C3, C5, the C3a receptor gene (C3AR1), and the C5a receptor gene (C5R1) and performed association studies in the Japanese population. The results of this SNP case-control study suggested an association between 4896C/T in the C3 gene and atopic childhood BA (P = 0.0078) as well as adult BA (P = 0.010). When patient data were stratified according to elevated total IgE levels, 4896C/T was more closely associated with adult BA (P = 0.0016). A patient-only association study suggested that severity of childhood BA was associated with 1526G/A of the C3AR1 gene (P = 0.0057). We identified a high-risk haplotype of the C3 gene for childhood (P = 0.0021) and adult BA (P = 0.0058) and a low-risk haplotype for adult BA (P = 0.00011). We also identified a haplotype of the C5 gene that was protective against childhood BA (P = 1.4 x 10(-6)) and adult BA (P = 0.00063). These results suggest that the C3 and C5 pathways of the complement system play important roles in the pathogenesis of BA and that polymorphisms of these genes affect susceptibility to BA.


Assuntos
Asma/genética , Complemento C3/genética , Complemento C5/genética , Variação Genética , Receptores de Complemento/genética , Adulto , Análise de Variância , Criança , Predisposição Genética para Doença/genética , Haplótipos/genética , Humanos , Japão , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA
7.
Hum Genet ; 114(5): 503-9, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15004750

RESUMO

Sarcoidosis is known to be a systemic granulomatous disorder characterized by a cell-mediated Th1-type inflammatory response. To identify a key genetic factor in the pathogenesis of sarcoidosis, we investigated single nucleotide polymorphisms within 10 candidate genes involved in type 1 immune process ( IFNA17, IFNB, IFNG, IFNGR1, IFNGR2, IL12B, IL12RB1, IL12RB2, ETA-1, and NRAMP1) in an association-based study of 102 Japanese patients with sarcoidosis, 114 with tuberculosis, and 110 control subjects. After correction for multiple testing, an IFNA17 polymorphism (551T-->G) was found to be associated with susceptibility to sarcoidosis (odds ratio 3.27 [95% CI: 1.44-7.46], P=0.004, P(c)=0.04), but not to tuberculosis. We observed no significant associations with the other polymorphisms of the Th1-related genes. We further typed another IFNA polymorphism ( IFNA10 60T-->A) and confirmed two major haplotypes of the IFNA gene, viz., allele 1: IFNA10 [60T]- IFNA17 [551T] and allele 2: IFNA10 [60A]- IFNA17 [551G], in the Japanese population. In healthy subjects, IFNA allele 2, which is over-represented in patients with sarcoidosis, was significantly associated with increased IFN-alpha and IL-12p70 production induced by Sendai virus in vitro. This study suggests that possession of the IFNA allele with higher levels of IFN-alpha significantly increases the risk of sarcoidosis.


Assuntos
Interferons/genética , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco , Sarcoidose/genética , Adolescente , Adulto , Idoso , Bioensaio , Estudos de Casos e Controles , Criança , Primers do DNA , Ensaio de Imunoadsorção Enzimática , Feminino , Frequência do Gene , Haplótipos/genética , Humanos , Interferon-alfa/sangue , Interferon-alfa/metabolismo , Interleucina-12/sangue , Interleucina-12/metabolismo , Japão , Masculino , Pessoa de Meia-Idade , Vírus Sendai/metabolismo
8.
J Allergy Clin Immunol ; 109(6): 980-7, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12063528

RESUMO

BACKGROUND: A substantial body of evidence exists to support the pivotal role of IL-13 in the pathogenesis of bronchial asthma. We recently found that a variant of the IL13 gene (Arg110Gln) is genetically associated with bronchial asthma, which is concordant with animal experiments using IL-13 in the development of asthma. OBJECTIVE: To address whether the Gln110 variant of IL13 influences IL-13 function, contributing to the pathogenesis of bronchial asthma, we studied the functional properties of the variant. METHODS: We generated 2 types of recombinant IL-13 proteins, the amino acids of which at 110 were arginine or glutamine, and analyzed the binding affinities with the IL-13 receptors, as well as the stability of the proteins. We further compared the relationship between the genotype and serum levels of IL-13. RESULTS: The variant showed a lower affinity with the IL-13 receptor alpha2 chain, a decoy receptor, causing less clearance. The variant also demonstrated an enhanced stability in both human and mouse plasma. We further identified that asthmatic patients homozygous for the Gln110 variant have higher serum levels of IL-13 than those without the variant. CONCLUSION: These results suggested that the variant might act as a functional genetic factor of bronchial asthma with a unique mechanism to upregulate local and systemic IL-13 concentration in vivo.


Assuntos
Asma/genética , Variação Genética , Interleucina-13/biossíntese , Regulação para Cima , Substituição de Aminoácidos , Asma/metabolismo , Linhagem Celular , Genótipo , Humanos , Interleucina-13/sangue , Interleucina-13/genética , Interleucina-13/fisiologia , Subunidade alfa1 de Receptor de Interleucina-13 , Receptores de Interleucina/metabolismo , Receptores de Interleucina-13 , Proteínas Recombinantes/metabolismo
9.
Biochem Biophys Res Commun ; 292(3): 776-80, 2002 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-11922633

RESUMO

Thromboxane A2 (TXA2) is an arachidonate metabolite which is considered to relate to chronic inflammation in atopic diseases characterized by elevated immunoglobulin E productivity. The elevation of immunoglobulin E levels involves many molecules including interleukin-4 (IL-4) and interleukin-4 receptor alpha chain (IL-4R alpha). To assess whether genetic variants of TXA2 receptor, IL-4 and IL-4R alpha genes relate to the elevation of serum immunoglobulin E levels in patients with atopic dermatitis (AD), we conducted an association study of genetic polymorphisms of TXA2 receptor (795C/T), IL-4 (-589C/T), and IL-4R alpha (Ile50Val) in a Japanese population (n = 789). The TXA2 receptor 795TT genotype strongly related to AD with high serum immunoglobulin E concentrations. AD patients with both TXA2 receptor 795TT genotype and the IL-4R alpha Ile50/Ile50 genotype showed the greatest immunoglobulin E concentrations. These results suggest TXA2 receptor polymorphism strongly interacts with IL-4R alpha polymorphism as a major determinant of high serum immunoglobulin E levels in AD.


Assuntos
Dermatite Atópica/genética , Imunoglobulina E/sangue , Receptores de Interleucina-4/genética , Receptores de Tromboxanos/genética , Adolescente , Adulto , Idoso , Criança , Dermatite Atópica/imunologia , Dermatite Atópica/fisiopatologia , Feminino , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético , Receptores de Interleucina-4/imunologia , Receptores de Tromboxanos/imunologia
10.
J Clin Invest ; 109(1): 51-8, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11781350

RESUMO

Proper control of cell cycle progression is critical for the constant self-renewal, differentiation, and homeostasis of the hematopoietic system. Cells of all types share the common cell cycle regulators. The different expression patterns of common regulators, in a broad sense, define cell-type or lineage specificity. However, there remains the possibility of hematopoietic cell cycle regulators tailored to the demands of the hematopoietic system. Here we describe a novel protein, HTm4, which serves as a hematopoietic cell cycle regulator. Our data indicate that HTm4 is expressed in hematopoietic tissues and is tightly regulated during the differentiation of hematopoietic stem cells. It binds to cyclin-dependent kinase-associated (CDK-associated) phosphatase-CDK2 (KAP-CDK2) complexes, and the three proteins demonstrate similar patterns of cellular expression in human lymphoid tissues. HTm4 stimulates the phosphatase activity of KAP, and its C-terminal region is required for binding to KAP-CDK2 complexes and the modulation of KAP activity. Overexpression of HTm4 can cause cell cycle arrest at the G(0)/G(1) phase. Thus, HTm4 is a novel hematopoietic modulator for the G(1)-S cell cycle transition.


Assuntos
Quinases relacionadas a CDC2 e CDC28 , Proteínas de Ciclo Celular , Ciclo Celular/fisiologia , Hematopoese/fisiologia , Proteínas de Membrana/fisiologia , Sequência de Aminoácidos , Células Cultivadas , Quinase 2 Dependente de Ciclina , Proteínas Inibidoras de Quinase Dependente de Ciclina , Quinases Ciclina-Dependentes/metabolismo , Fosfatases de Especificidade Dupla , Regulação da Expressão Gênica , Células-Tronco Hematopoéticas/citologia , Células-Tronco Hematopoéticas/fisiologia , Humanos , Imuno-Histoquímica , Proteínas de Membrana/genética , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Tirosina Fosfatases/metabolismo , Transfecção , Técnicas do Sistema de Duplo-Híbrido
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