1.
Curr Protoc Hum Genet
; Chapter 18: Unit 18.4, 2010 Jul.
Artigo
em Inglês
| MEDLINE
| ID: mdl-20582916
RESUMO
This unit describes a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in-solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool are reviewed, as are critical steps for performing the preparation and hybrid selection of an Illumina paired-end library. Critical parameters, performance metrics, and analysis workflow are discussed.