Respiratory function in uncomplicated type 1 diabetes: Blunted during exercise even though normal at rest!

AIMS: Type 1 diabetes is associated with a substantially increased risk of impaired lung function, which may impair aerobic fitness. We therefore aimed to examine the ventilatory response during maximal exercise and the pulmonary diffusion capacity function at rest in individuals with uncomplicated type 1 diabetes. METHODS: In all, 17 adults with type 1 diabetes free from micro-macrovascular complications (glycated haemoglobin: 8.0 ± 1.3%), and 17 non-diabetic adults, carefully matched to the type 1 diabetes group according to gender, age, level of physical activity and body composition, participated in our study. Lung function was assessed by spirometry and measurements of the combined diffusing capacity for nitric oxide (DLNO) and carbon monoxide (DLCO) at rest. Subjects performed a maximal exercise test during which the respiratory parameters were measured. RESULTS: At rest, DLCO (30.4 ± 6.1 ml min-1  mmHg-1 vs. 31.4 ± 5.7 ml min-1 mmHg-1 , respectively, p = 0.2), its determinants Dm (membrane diffusion capacity) and Vc (pulmonary capillary volume) were comparable among type 1 diabetes and control groups, respectively. Nevertheless, spirometry parameters (forced vital capacity = 4.9 ± 1.0 L vs. 5.5 ± 1.0 L, p < 0.05; forced expiratory volume 1 = 4.0 ± 0.7 L vs. 4.3 ± 0.7 L, p < 0.05) were lower in individuals with type 1 diabetes, although in the predicted normal range. During exercise, ventilatory response to exercise was different between the two groups: tidal volume was lower in type 1 diabetes vs. individuals without diabetes (p < 0.05). Type 1 diabetes showed a reduced VO2max (34.7 ± 6.8 vs. 37.9 ± 6.3, respectively, p = 0.04) in comparison to healthy subjects. CONCLUSIONS: Individuals with uncomplicated type 1 diabetes display normal alveolar-capillary diffusion capacity and at rest, while their forced vital capacity, tidal volumes and VO2 are reduced during maximal exercise.

Muscle Oxygen Supply and Use in Type 1 Diabetes, From Ambient Air to the Mitochondrial Respiratory Chain: Is There a Limiting Step?

OBJECTIVE: Long before clinical complications of type 1 diabetes (T1D) develop, oxygen supply and use can be altered during activities of daily life. We examined in patients with uncomplicated T1D all steps of the oxygen pathway, from the lungs to the mitochondria, using an integrative ex vivo (muscle biopsies) and in vivo (during exercise) approach. RESEARCH DESIGN AND METHODS: We compared 16 adults with T1D with 16 strictly matched healthy control subjects. We assessed lung diffusion capacity for carbon monoxide and nitric oxide, exercise-induced changes in arterial O2 content (SaO2, PaO2, hemoglobin), muscle blood volume, and O2 extraction (via near-infrared spectroscopy). We analyzed blood samples for metabolic and hormonal vasoactive moieties and factors that are able to shift the O2-hemoglobin dissociation curve. Mitochondrial oxidative capacities were assessed in permeabilized vastus lateralis muscle fibers. RESULTS: Lung diffusion capacity and arterial O2 transport were normal in patients with T1D. However, those patients displayed blunted exercise-induced increases in muscle blood volume, despite higher serum insulin, and in O2 extraction, despite higher erythrocyte 2,3-diphosphoglycerate. Although complex I- and complex II-supported mitochondrial respirations were unaltered, complex IV capacity (relative to complex I capacity) was impaired in patients with T1D, and this was even more apparent in those with long-standing diabetes and high HbA1c. [Formula: see text]O2max was lower in patients with T1D than in the control subjects. CONCLUSIONS: Early defects in microvascular delivery of blood to skeletal muscle and in complex IV capacity in the mitochondrial respiratory chain may negatively impact aerobic fitness. These findings are clinically relevant considering the main role of skeletal muscle oxidation in whole-body glucose disposal.

Muscle oxygen supply impairment during exercise in poorly controlled type 1 diabetes.

PURPOSE: Aerobic fitness, as reflected by maximal oxygen (O2) uptake (VO2max), is impaired in poorly controlled patients with type 1 diabetes. The mechanisms underlying this impairment remain to be explored. This study sought to investigate whether type 1 diabetes and high levels of glycated hemoglobin (HbA1c) influence O2 supply including O2 delivery and release to active muscles during maximal exercise. METHODS: Two groups of patients with uncomplicated type 1 diabetes (T1D-A, n = 11, with adequate glycemic control, HbA1c <7.0%; T1D-I, n = 12 with inadequate glycemic control, HbA1c >8%) were compared with healthy controls (CON-A, n = 11; CON-I, n = 12, respectively) matched for physical activity and body composition. Subjects performed exhaustive incremental exercise to determine VO2max. Throughout the exercise, near-infrared spectroscopy allowed investigation of changes in oxyhemoglobin, deoxyhemoglobin, and total hemoglobin in the vastus lateralis. Venous and arterialized capillary blood was sampled during exercise to assess arterial O2 transport and factors able to shift the oxyhemoglobin dissociation curve. RESULTS: Arterial O2 content was comparable between groups. However, changes in total hemoglobin (i.e., muscle blood volume) was significantly lower in T1D-I compared with that in CON-I. T1D-I also had impaired changes in deoxyhemoglobin levels and increase during high-intensity exercise despite normal erythrocyte 2,3-diphosphoglycerate levels. Finally, VO2max was lower in T1D-I compared with that in CON-I. No differences were observed between T1D-A and CON-A. CONCLUSIONS: Poorly controlled patients displayed lower VO2max and blunted muscle deoxyhemoglobin increase. The latter supports the hypotheses of increase in O2 affinity induced by hemoglobin glycation and/or of a disturbed balance between nutritive and nonnutritive muscle blood flow. Furthermore, reduced exercise muscle blood volume in poorly controlled patients may warn clinicians of microvascular dysfunction occurring even before overt microangiopathy.

Cadence and exercise: physiological and biomechanical determinants of optimal cadences--practical applications.

This critical review reflects the current state of cadence research during cyclical activities at different intensities. Moreover, this review aims at making suggestions in the areas of evaluation, therapy and sporting performance in the light of all the different results reported in studies. A large number of researchers have tried to determine the 'optimal' cadence from imposed, preferred or spontaneously chosen cadences in order to improve efficiency and performances. Results are sometimes conflicting and difficult to explain or interpret. The authors have studied the variations in cadences using a reduced number of parameters, without links between energetic (oxygen consumption, ventilation), biomechanical (force, electromyography) and/or perceived parameters (rating of perceived exertion). Conclusions point out that the 'optimal' cadence cannot be unique and must be associated with the objectives and individual characteristics of the subject (skills and training level, anthropometric parameters). In the area of training and reconditioning, cadences would have to be set in relation to the nature of cyclical activities and the subjects' condition.

Cardiorespiratory and efficiency responses during arm and leg exercises with spontaneously chosen crank and pedal rates.

The aim of this study was to compare the cardiorespiratory and efficiency responses between upper (T(UBE)) and lower (T(LBE)) body exercises at the same relative power outputs and with spontaneously chosen crank (SCCR) or pedal (SCPR) rates. Twelve participants performed exercise bouts set at 20, 40, 60 and 80% of maximal power (MP) separated by passive recovery periods. Oxygen uptake, ventilation, gross and work efficiencies during T(LBE) were significantly (P < 0.05) higher than during T(UBE). These results suggest that these responses were not directly related to the relative intensities. However, no significant difference was found for delta efficiency and heart rate values. During T(UBE) and T(LBE), gross efficiency increased significantly (P < of MP for T(UBE) and T(LBE) and the same SCCR and SCPR could explain these results. The present results confirm that the cardiorespiratory and efficiency responses between arm and leg exercises are not always similar, although the power output are normalized in relation to MP and add to the understanding of differences between upper and lower body.

Ventilatory thresholds in arm and leg exercises with spontaneously chosen crank and pedal rates.

The present study assessed whether the first and the second ventilatory thresholds (VT1 and VT2) were dependent on the muscle groups solicited when spontaneously chosen crank and pedal rates are used. 20 physical education male students (22 +/- 2.2 yr.) performed two maximal incremental tests randomly assigned using an increment of 15 and 30 W every minute for arm and leg exercises, respectively. These tests were used to measure the maximal oxygen uptake (VO2 max) and to identify VT1 and VT2. The absolute oxygen uptake (VO2) values measured at VT1, VT2, and at maximal workload were significantly (p < .05) lower during arm and leg exercises. However, VT1 and VT2 expressed in percent of VO2 max were not significantly different between arm and leg exercises (54.1 +/- 8.2 vs 57.2 +/- 11.4%; and 82.5 +/- 6.4 vs 84.6 +/- 5.1% at VT1 and VT2, respectively). In addition, at the two thresholds, none of the variables measured during arm and leg exercises were significantly correlated with the exception of spontaneously chosen crank and pedal rates (p < .01; r = .75 and r = .69 for VT1 and VT2, respectively). Probably due to the different training status and skill level, no extrapolation can be made to specify the arm thresholds from the leg. These results underline the need to specify the ventilatory thresholds from specific arm ergometer measures obtained from tests performed with spontaneously chosen crank and pedal rates and, thus, close to sport and recreational activities, when they are used for training and rehabilitation programs.