Marine shrimps as biomonitors of the Fundão (Brazil) mine dam disaster: A multi-biomarker approach.

The disruption of the Fundão dam released 43 million m3 of mine tailings into the Doce River until it flowed into the ocean through the estuary. The mine tailing changed the composition of metals in water and sediment, creating a challenging scenario for the local biota. We used multivariate analyzes and the integrated biomarker response index (IBR) to assess the impact of mine tailings on the bioaccumulation profile (As, Cd, Cr, Cu, Fe, Mn, Pb and Zn) as well as the biomarkers response in gills, hepatopancreas and muscle of shrimps sampled from different sectors during two dry seasons (dry1 and dry2) (Sep/Oct 2018; 2019) and two wet seasons (wet1 and wet2) (Jan/feb 2019; 2020). There was seasonal and local effect under bioaccumulation and biomarker response revealing that the pattern responses seen in each sector sampled changed according to the season. The greater IBR added to the strong association among the most metals tissue content (Cd, Cr, Cu and Mn) and sectors sampled during dry 1 suggests greater bioavailability of these metals to the environment in this period. Estuarine sectors stand out for high Fe bioavailability, especially during wet1, which seems to be associated with greater metallothionein content in hepatopancreas of shrimps. Native species of marine shrimps proved to be successful indicators of sediment quality besides being sensitive to water contamination by metals. The multi-biomarkers approach added to multivariate analysis supports the temporal and seasonal effects, signalizing the importance of continuous monitoring of the estuarine region to better know about the bioavailability of these metals, mainly Fe, and their long-term effects on the local biota.

Biochemical response and metals bioaccumulation in planktonic communities from marine areas impacted by the Fundão mine dam rupture (southeast Brazil).

The rupture of the Fundão dam (Mariana, MG, southeast Brazil) released a huge flood of mine tailings to Doce river basin and its adjacent coastal area, in November 2015. This catastrophic event exposed aquatic communities to metal contamination related to mine tailings, but its biological effects are still poorly understood. This study investigates how biochemical response related to metal exposure vary between locations and seasons during the years of 2018-2020, in planktonic communities (micro and mesoplankton). Marine microplankton collected in sectors in front and south of the Doce river mouth presented the highest lipid peroxidation (LPO) and induction of metallothioneins (MT). Mesoplankton collected in sectors in front and north of the Doce river mouth presented highest LPO, while MT in this size class did not respond to a clear spatial pattern. Our results showed that metals affected biomarkers in a non-linear pattern and highlighted the complex relationship between metals, biochemical parameters, and seasonality. The variation in biochemical biomarkers indicates physiological stress related to metals, once sectors contaminated by metals, especially Fe, Mn and Cd, presented stronger biochemical responses. Comparison of metal levels with bioaccumulation data collected before the impact indicates Fe, Cd, Cr and Cu more than 2-fold higher after disaster in sectors closer to the river. Literature showed that these sectors present zooplanktonic assemblages with lower biomass and biodiversity, suggesting that the opportunistic species that thrives in the area are also under biochemical stress, but possibly relies on repair or defense mechanisms. The physiological stress detected by this study is possibly related to the mine tailings, considering the metals that stood out and the proximity with the Doce river mouth. This suggests that the impacts related to the failure of Fundão dam are still affecting the marine planktonic community even three to four years after the environmental disaster.

Late rise in hemolymph osmolality in Macrobrachium acanthurus (diadromous freshwater shrimp) exposed to brackish water: Early reduction in branchial Na+/K+ pump activity but stable muscle HSP70 expression.

Some Macrobrachium shrimps (Caridea, Palaemonidae) are diadromous; freshwater adults are truly euryhaline, while larvae need saline water for development. Branchial Na+/K+-ATPase (NKA) and carbonic anhydrase (CA) are involved in NaCl absorption in freshwater. This study aimed at verifying the time course of the osmoregulatory response of adult Macrobrachium acanthurus to high salinity brackish water (20‰), from the first 30min to 5days. The goal was to detect possible transition from hyper- to hyporegulation, the putative involvement of branchial NKA and CA, or the induction of muscular HSP70 expression. Hemolymph osmotic and ionic concentrations remained relatively stable and close to control levels until ~9h of exposure, but later increased consistently (~50%). A fast reduction in NKA activity (3-6h) was observed; these shrimps seem to shut off salt absorption already in the first hours. Later on, especially after 24h, hemolymph concentrations rise but HSP70 expression is not induced, possibly because constitutive levels are already sufficient to prevent protein damage. Time-dependent response mechanisms effective in high salinity brackish water, resulting in salt loading avoidance and suggestive of hyporegulation should be further investigated in decapods that evolutionary invaded freshwater.