Top-dressing 1% arginine supplementation in the lactation diet of sows does not affect the litter performance and milk composition / A suplementação de 1% de arginina via top-dress na dieta de lactação de porcas não afeta o desempenho das leitegadas e a composição do leite

ABSTRACT: The study aimed to evaluate the effects of arginine supplementation in the lactation diet of sows on their milk composition, litter performance and piglet survival. Sixty-four lactating Landrace x Large White sows, parity 1 to 7, were randomly assigned to two treatments: 1) Control - a corn/soybean meal based diet with 1.10% standardized ileal digestible (SID) lysine and 3,475kcal of metabolizable energy (ME) kg-1, and 2) arginine - the control diet top-dressed daily with arginine at 1% of feed allowance. The daily feed allowance per sow was 5.0 and 7.5kg from day (D)0 to D7 and D8 to D21, respectively. The average litter size was 12.8 piglets after cross-fostering. Litters were weighed on D1, D10, and D21 of lactation and pre-weaning mortality was recorded. Samples of milk (60mL) were collected from all functional teats at D10 and D20 of lactation. There were no effects (P>0.05) of arginine supplementation on piglet weight, litter weight, and average daily gain of piglets at D10 and D21 of lactation. The interaction between weight day and treatment was not significant (P>0.05) for any of these response variables. The percentages of piglets that survived until D10 and D21 were 90.3% and 88.3%, respectively, with no difference (P>0.05) between treatments. There were no effects (P>0.05) of the lactation day (D10 or D20), treatment or the interaction between them on crude protein and amino acid content in milk. Top-dressing arginine at 1% of feed allowance of the lactation diet of sows does not affect litter performance and survival and does not influence the amino acid content or arginine: lysine ratio of milk.

RESUMO: O estudo teve o objetivo de avaliar o efeito da suplementação de arginina na dieta de lactação de porcas sobre a composição do leite, desempenho da leitegada e sobrevivência dos leitões. Foram utilizadas 64 porcas lactantes Landrace - Large White, com parição de 1 a 7, aleatoriamente distribuídas em dois tratamentos: 1) Controle - dieta a base de milho e soja com 1,10% de lisina digestível e 3.475kcal de energia metabolizável; e 2) Arginina - dieta controle suplementada com arginina via top-dress em nível de 1% sobre a dieta fornecida. Diariamente, as matrizes receberam 5,0 e 7,5kg do D0 ao D7 e D8 ao D21, respectivamente. Após a uniformização das leitegadas, o número médio de lactentes foi de 12,8 leitões. As leitegadas foram pesadas no D1, D10 e D21 da lactação e a mortalidade pré-desmame foi registrada. Foram coletadas amostras de leite (60mL) de todas as tetas funcionais de cada porca no D10 e no D20 da lactação. Não houve efeito (P>0,05) da suplementação de arginina sobre o peso dos leitões, peso da leitegada e ganho de peso médio diário dos leitões no D10 e D21 da lactação. A interação entre dia da pesagem e tratamento não foi significativa (P>0,05) para as variáveis-resposta analisadas. Os percentuais de leitões vivos até o D10 e D21 foram de 90,3% e 88,3%, respectivamente, sem diferença (P>0,05) entre os tratamentos. Não houve efeito (P>0,05) do dia de lactação (D10 ou D20), tratamento ou interação entre eles na proteína bruta e conteúdo de aminoácidos do leite. A suplementação de arginina em nível de 1% da dieta fornecida diariamente não teve efeito sobre o desempenho e sobrevivência da leitegada e não influenciou no conteúdo de aminoácidos ou na relação arginina:lisina do leite.

Effects of transmembrane region variability on cell surface expression and allorecognition of HLA-DP3.

The functional relevance of polymorphisms outside the peptide binding groove of HLA molecules is poorly understood. Here we have addressed this issue by studying HLA-DP3, a common antigen relevant for functional matching algorithms of unrelated hematopoietic stem cell transplantation (HSCT) encoded by two transmembrane (TM) region variants, DPB1(*)03:01 and DPB1(*)104:01. The two HLA-DP3 variants were found at a overall allelic frequency of 10.4% in 201 volunteer stem cell donors, at a ratio of 4.2:1. No significant differences were observed in cell surface expression levels of the two variants on B lymphoblastoid cell lines (BLCL), primary B cells or monocytes. Three different alloreactive T cell lines or clones showed similar levels of activation marker CD107a and/or CD137 upregulation in response to HLA-DP3 encoded by DPB1(*)03:01 and DPB1(*)104:01, either endogenously on BLCL or after lentiveral-vector mediated transfer into the same cellular background. These data provide, for the first time, direct evidence for a limited functional role of a TM region polymorphism on expression and allorecognition of HLA-DP3 and are compatible with the notion that the two variants can be considered as a single functional entity for unrelated stem cell donor selection.

Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation.

The importance of donor-recipient human leukocyte antigen (HLA)-DPB1 matching for the clinical outcome of unrelated hematopoietic stem cell transplantation (HSCT) is controversial. We have previously described an algorithm for nonpermissive HLA-DPB1 disparities involving HLA-DPB1*0901,*1001,*1701,*0301,*1401,*4501, based on T-cell alloreactivity patterns. By revisiting the immunogenicity of HLA-DPB1*02, a modified algorithm was developed and retrospectively tested in 621 unrelated HSCTs facilitated through the Italian Registry for oncohematologic adult patients. The modified algorithm proved to be markedly more predictive of outcome than the original one, with significantly higher Kaplan-Meier probabilities of 2-year survival in permissive compared with nonpermissive transplantations (55% vs 39%, P = .005). This was the result of increased adjusted hazards of nonrelapse mortality (hazard ratio [HR] = 1.74; confidence interval [CI], 1.19-2.53; P = .004) but not of relapse (HR = 1.02; CI, 0.73-1.42; P = .92). The increase in the hazards of overall mortality by nonpermissive HLA-DPB1 disparity was similar in 10 of 10 (HR = 2.12; CI, 1.23-3.64; P = .006) and 9 of 10 allele-matched transplantations (HR = 2.21; CI, 1.28-3.80; P = .004), both in early-stage and in advanced-stage disease. These data call for revisiting current HLA matching strategies for unrelated HSCT, suggesting that searches should be directed up-front toward identification of HLA-DPB1 permissive, 10 of 10 or 9 of 10 matched donors.

Age-dependent vascular changes induced by status epilepticus in rat forebrain: implications for epileptogenesis.

Brain inflammation, angiogenesis and increased blood-brain barrier (BBB) permeability occur in adult rodent and human epileptogenic brain tissue. We addressed the role of these events in epileptogenesis using a developmental approach since the propensity to develop spontaneous seizures, therefore the induction of epileptogenesis, is age-dependent and increases with brain maturation. Inflammation, angiogenesis and BBB permeability were studied in postnatal day (PN)9 and PN21 rats, 1 week and 4 months after pilocarpine-induced status epilepticus. Brain inflammation was evaluated by interleukin(IL)-1beta immunohistochemistry; angiogenesis was quantified by measuring the density of microvessels identified by an anti-laminin antibody or by the intraluminal signal of FITC-albumin; BBB integrity was assessed by extravascular IgG immunostaining or detection of parenchymal extravasation of FITC-albumin. Neither inflammation nor angiogenesis or changes in BBB permeability were detected in PN9 rats after status epilepticus, and these rats did not develop spontaneous seizures in adulthood as assessed by video-EEG monitoring. Differently, status epilepticus in PN21 rats induced chronic inflammation, angiogenesis and BBB leakage in the hippocampus in 62% of rats, while in the remaining rats only transient inflammation in forebrain was observed. Epilepsy developed in about 62% of PN21 rats exposed to SE and these epileptic rats showed the three phenomena concomitantly in the hippocampus. PN21 rats that did not develop epilepsy 4 months after status epilepticus, as assessed by video-EEG monitoring, they did not show inflammation, angiogenesis or BBB damage in forebrain at this time. Our data show that age-dependent vascular changes and brain inflammation induced by status epilepticus are associated with epileptogenesis, suggesting that these phenomena are implicated in the mechanisms underlying the occurrence of spontaneous seizures.

Assessing the influence of recollection and familiarity in memory for own- versus other-race faces.

In the present research, we examined the contributions of recollection and familiarity in memory for own- and other-race faces. In Experiment 1, we used a repetition lag paradigm (Jennings & Jacoby, 1997) to demonstrate the typical cross-race effect with respect to discrimination accuracy and response bias. Participants were more likely to commit repetition errors by falsely recognizing repeated other-race faces. In Experiment 2, we used process-dissociation equations to estimate differences in recollection and familiarity. As predicted, results showed a greater reliance on recollection-based processing for own-race faces. The theoretical and practical implications of these findings are discussed.