Hematology and Serum Biochemistry of Coastal Seabirds Rehabilitated on the Southeastern and Southern Coast of Brazil.

Complete blood counts (n=566) and serum biochemistry (n=426) were assessed in seven coastal seabirds species that underwent rehabilitation along the southeastern and southern coast of Brazil from Saquarema, Rio de Janeiro State (22°56'16.44″S, 42°18'24.16″W) to Laguna, Santa Catarina State (28°29'43″S, 48°45'39.2″W), from August 2016 to August 2020. Blood samples were collected from four species of Charadriiformes, including Kelp Gull (Larus dominicanus, n=136), South American Tern (Sterna hirundinacea, n=25), Cabot's Tern (Thalasseus acuflavidus, n=17), and Common Tern (Sterna hirundo, n=14) as well as three species of Suliformes, the Brown Booby (Sula leucogaster, n=212), Magnificent Frigatebird (Fregata magnificens, n=104), and Neotropic Cormorant (Nannopterum brasilianum, n=58). The individuals were sampled as part of the protocol required before their release into the wild when considered healthy. This work aimed to establish the normal hematologic and biochemical reference values of those seabird species and, when possible, to analyze variations among age class and sex and to compare those with the available data in the literature. In addition, we provide the first baseline data for the South American Tern, Cabot's Tern, and Neotropic Cormorant. Baseline hematologic data are crucial for assessing health status of individuals and to support management and conservation actions, including release of seabirds into the wild.

Ultrasound examination of coelomic viscera through the plastron in stranded green sea turtles (Chelonia mydas).

Many projects have been developed in the last years for the conservation of sea turtles. Young green turtles (Chelonia mydas) often nest on the Brazilian coast. Because they nest in beaches along the coastline and islands, green turtles are susceptible to fishing and accidental ingestion of anthropogenic debris. Early detection of ingested debris is crucial for the survival of rescued sea animals. Ultrasound (US) has emerged as a viable imaging technique for visceral examination in veterinary medicine. Previous studies have suggested the left and right cervicobrachial, axillary, pre- and post-femoral areas as the only viable approaches for US examination, but the acoustic windows available for imaging of coelomic structures are limited. It is important to notice that a detailed evaluation of all gastrointestinal tracts, especially the duodenum, is crucial for detecting foreign bodies and intestinal obstructive processes, as well as obtaining essential information such as intestinal motility and heart frequency. Intestinal motility and heartbeats are not detected through radiographic examination or through the acoustic windows available so far. This study aimed to establish the viability of US examination of coelomic viscera through the plastron in stranded green turtles. Eleven young green turtles rescued by the GREMAR Institute were examined. Turtles were placed in the dorsal decubitus position during US examination, which did not require anesthesia. Even though the plastron is constituted of bones and cartilage, the present research has proven the viability of obtaining US images through it, making it possible to visualize structures undetectable through the acoustic windows previously suggested. The following organs were evaluated through the plastron: heart, stomach, duodenum, jejunum, colon, liver, gallbladder, kidneys, bladder, and spleen (in case of splenomegaly). US imaging through the plastron is a viable approach for detecting ingested artificial debris, which represents one of the leading causes of death among sea turtles.