Prenatal cocaine exposure and its influence on pediatric epigenetic clocks and epigenetic scores in humans.

The investigation of the effects of prenatal cocaine exposure (PCE) on offspring has been inconsistent, with few studies investigating biological outcomes in humans. We profiled genome-wide DNA methylation (DNAm) of umbilical cord blood (UCB) from newborns with (n = 35) and without (n = 47) PCE. We used DNAm data to (1) assess pediatric epigenetic clocks at birth and (2) to estimate epigenetic scores (ES) for lifetime disorders. We generated gestational epigenetic age estimates (DNAmGA) based on Knight and Bohlin epigenetic clocks. We also investigated the association between DNAmGA and UCB serum brain-derived neurotrophic factor (BDNF) levels. Considering the large-scale DNAm data availability and existing evidence regarding PCE as a risk for health problems later in life, we generated ES for tobacco smoking, psychosis, autism, diabetes, and obesity. A gene ontology (GO) analysis on the CpGs included in the ES with group differences was performed. PCE was associated with lower DNAmGA in newborns, and this effect remained significant when controlling for potential confounders, such as blood cell type composition predicted by DNAm and obstetric data. DNAmGA was negatively correlated with BDNF levels in the serum of UCB. Higher tobacco smoking, psychosis, and diabetes ES were found in the PCE group. The GO analysis revealed GABAergic synapses as a potential pathway altered by PCE. Our findings of decelerated DNAmGA and ES for adverse phenotypes associated with PCE, suggest that the effects of gestational cocaine exposure on the epigenetic landscape of human newborns are detectable at birth.

Oxytocin Receptor Exon III Methylation in the Umbilical Cord Blood of Newborns With Prenatal Exposure to Crack Cocaine.

BACKGROUND: Prenatal cocaine exposure (PCE) is associated with behavioral, cognitive, and social consequences in children that might persist into later development. However, there are still few data concerning epigenetic mechanisms associated with the effects of gestational cocaine exposure, particularly in human newborns. AIMS: We investigated the effects of PCE on DNA methylation patterns of the Oxytocin Receptor (OXTR) gene in the umbilical cord blood (UCB). The relationship between UCB DNA methylation levels and the severity of the mother's cocaine use during pregnancy was also evaluated. METHODS: In this cross-sectional study, 28 UCB samples of newborns with a history of crack cocaine exposure in utero and 30 UCB samples of non-exposed newborns (NEC) were compared for DNA methylation levels at two genomic loci located in exon III of the OXTR gene (OXTR1 and OXTR2) through pyrosequencing. Maternal psychopathology was investigated using the Mini International Neuropsychiatric Interview, and substance use characteristics and addiction severity were assessed using the Smoking and Substance Involvement Screening Test (ASSIST). RESULTS: No differences between newborns with a history of PCE and NEC were observed in OXTR1 or OXTR2 DNA methylation levels. However, regression analyses showed that maternal addiction severity for crack cocaine use predicted OXTR1 DNA methylation in newborns. CONCLUSION: These data suggest that OXTR methylation levels in the UCB of children are affected by the severity of maternal crack cocaine usage. Larger studies are likely to detect specific changes in DNA methylation relevant to the consequences of PCE.

Increased cocaine and amphetamine-regulated transcript cord blood levels in the newborns exposed to crack cocaine in utero.

BACKGROUND: Cocaine and amphetamine-regulated transcript (CART) is an endogenous antioxidant present since the embryonic period. CART is activated by high levels of dopamine and might be of interested in understanding the changes in the REDOX system associated with crack/cocaine intake. The goal of this study was to determine whether exposure to crack in utero is associated with increased CART levels. METHODS: In this cross-sectional study with consecutive sampling, we compared the umbilical cord blood (UCB) CART levels (µg/mL) of newborns exposed to crack/cocaine in utero (EN, n = 57) to levels in non-exposed newborns (NEN, n = 99). In addition, we compared serum CART levels between EN and NEN mothers, in the immediate postpartum period. Potential confounders, such as perinatal data (e.g., weight, Apgar, etc.), psychopathology (DSM-IV), and use of drugs other than crack (ASSIST) were assessed. RESULTS: According to general linear model analysis, the adjusted mean CART was significantly higher in EN (0.180, 95% CI 0.088-0.272) than in NEN (0.048, 95% CI 0.020-0.076; p < 0.002; d = 0.68). The difference in CART levels between EN and NEN mothers was not significant (p ≥ 0.05). CONCLUSION: The increase in CART levels in EN UBC suggests a response to crack/cocaine-induced oxidative stress during gestational period, as a potential attempt of neuroprotection. In adult women in puerperium, however, this endogenous antioxidant recruitment does not seem to operate.

TBARS and BDNF levels in newborns exposed to crack/cocaine during pregnancy: a comparative study

Objectives: To compare levels of a marker of lipid peroxidation (thiobarbituric acid reactive substances, TBARS) and brain-derived neurotrophic factor (BDNF) in umbilical cord blood (UCB) between newborns exposed to crack/cocaine in utero (exposed newborns [EN], n=57) and non-exposed newborns (NEN, n=99), as well as in maternal peripheral blood at delivery. Methods: This was a cross-sectional study. Potential confounders, including perinatal parameters, psychopathology, and use of other substances, were assessed. Results: After adjusting for potential confounders, adjusted mean BDNF was significantly higher in EN (3.86 ng/mL, 95% confidence interval [95%CI] 2.29-5.43) than in NEN (0.85 ng/mL, 95%CI 0.47-1.23; p < 0.001; Cohen effect size: 1.12), and significantly lower in crack/cocaine mothers than in control mothers (4.03 ng/mL, 95%CI 2.87-5.18 vs. 6.67 ng/mL, 95%CI 5.60-7.74; p = 0.006). The adjusted mean TBARS level was significantly lower in EN (63.97 µM MDA, 95%CI 39.43-88.50) than NEN (177.04 µM MDA, 95%CI 140.93-213.14; p < 0.001; effect size = 0.84), with no difference between mother groups (p = 0.86). Conclusions: The changes in TBARS levels observed in EN suggest that fetuses exposed to cocaine mobilize endogenous antioxidant routes since very early stages of development. The increase in BDNF levels in EN might indicate changes in fetal development, whereas the changes in BDNF levels in mothers provide evidence of the complex metabolic processes involved in drug use during pregnancy.

TBARS and BDNF levels in newborns exposed to crack/cocaine during pregnancy: a comparative study.

OBJECTIVES:: To compare levels of a marker of lipid peroxidation (thiobarbituric acid reactive substances, TBARS) and brain-derived neurotrophic factor (BDNF) in umbilical cord blood (UCB) between newborns exposed to crack/cocaine in utero (exposed newborns [EN], n=57) and non-exposed newborns (NEN, n=99), as well as in maternal peripheral blood at delivery. METHODS:: This was a cross-sectional study. Potential confounders, including perinatal parameters, psychopathology, and use of other substances, were assessed. RESULTS:: After adjusting for potential confounders, adjusted mean BDNF was significantly higher in EN (3.86 ng/mL, 95% confidence interval [95%CI] 2.29-5.43) than in NEN (0.85 ng/mL, 95%CI 0.47-1.23; p < 0.001; Cohen effect size: 1.12), and significantly lower in crack/cocaine mothers than in control mothers (4.03 ng/mL, 95%CI 2.87-5.18 vs. 6.67 ng/mL, 95%CI 5.60-7.74; p = 0.006). The adjusted mean TBARS level was significantly lower in EN (63.97 µM MDA, 95%CI 39.43-88.50) than NEN (177.04 µM MDA, 95%CI 140.93-213.14; p < 0.001; effect size = 0.84), with no difference between mother groups (p = 0.86). CONCLUSIONS:: The changes in TBARS levels observed in EN suggest that fetuses exposed to cocaine mobilize endogenous antioxidant routes since very early stages of development. The increase in BDNF levels in EN might indicate changes in fetal development, whereas the changes in BDNF levels in mothers provide evidence of the complex metabolic processes involved in drug use during pregnancy.

Comparação dos níveis séricos da Cocaine and Amphetamine Regulated Transcript (CART) entre sangue de cordão umbilical e sangue periférico em gestantes usuárias de crack / Comparison between serum levels of Cocaine and Amphetamine Regulated Transcript (CART) between umbilical cord blood and peripheral blood of pregnant crack users

INTRODUÇÃO: No Brasil, o uso de crack permanece um desafio à saúde pública devido à facilidade de aquisição da droga e sua elevada capacidade de induzir dependência. A exposição intrauterina (EIU) à cocaína está associada a alterações neurocomportamentais durante a infância e adolescência. Em estudo prévio do nosso grupo, achou-se menor nível de estresse oxidativo (EO) em recém-nascidos (RN) com EIU. Uma possível explicação pode ser a Cocaine and Amphetamine Regulated Transcript (CART), um antioxidante endógeno presente desde o período embrionário e ativado por maiores níveis de dopamina. OBJETIVO: Verificar a correlação entre os níveis de CART no sangue de cordão umbilical (SCU) e sangue periférico de 57 gestantes com exposição ao crack. MÉTODOS: Trata-se de um estudo transversal, com amostragem consecutiva, em que o desfecho primário foi a correlação entre os níveis de CART no SCU e sangue periférico materno no pós-parto imediato. Dados gestacionais e perinatais foram sistematicamente coletados. RESULTADOS: Houve correlação significativa entre os níveis de CART no sangue de cordão umbilical e sangue periférico materno (rs= 0,350 e p<0,05). CONCLUSÕES: Estes achados demonstram que os níveis de CART no sangue materno e no SCU se correlacionam. Todavia, não se pode afirmar de quem é a produção, ou se é produzida por ambos. O presente trabalho pode ajudar a elucidar os caminhos neurobiológicos responsáveis pelas alterações de neurodesenvolvimento, contribuindo para a ampliação das possibilidades de intervenções precoces.

INTRODUCTION: The use of crack cocaine remains a public health challenge in Brazil, due to easy drug acquisition and its high ability to induce dependence. Intrauterine exposure (IUE) to crack cocaine is associated with neurobehavioral changes during childhood and adolescence. In a previous study of our group, lower levels of oxidative stress (OS) were found in newborns with IUE. One possible explanation may be the Cocaine and Amphetamine Regulator Transcript (CART), an endogenous antioxidant present since the embryonic period activated by higher levels of dopamine. OBJECTIVE: The aim of this study is to investigate the correlation of CART levels between umbilical cord blood (UCB) and peripheral blood samples of 57 pregnant women exposed to crack. METHODS: This is a cross-sectional study with a consecutive sampling, in which the primary outcome was the correlation between CART levels in UCB and peripheral blood of their mothers in immediate postpartum. Gestational and perinatal data were systematically collected. Spearman correlation test was performed after checking the pattern of distribution, being considered a 0.05 significance level. RESULTS: There was a significant correlation between CART levels in umbilical cord blood and peripheral blood (rs = 0.350 and p <0.05). CONCLUSIONS: These findings suggest a correlation between CART levels at UCB and mother's blood. However, it remains unclear whether it is produced by the mother, the fetus, or both. This study may help to elucidate the neurobiological pathways responsible for neurodevelopmental changes, providing a rationale for early interventions.

IL-6 and IL-10 levels in the umbilical cord blood of newborns with a history of crack/cocaine exposure in utero: a comparative study.

INTRODUCTION: Prenatal cocaine exposure (PCE) is associated with neurobehavioral problems during childhood and adolescence. Early activation of the inflammatory response may contribute to such changes. Our aim was to compare inflammatory markers (IL-6 and IL-10) both in umbilical cord blood and in maternal peripheral blood at delivery between newborns with history of crack/cocaine exposure in utero and non-exposed newborns. METHODS: In this cross-sectional study, 57 newborns with a history of crack/cocaine exposure in utero (EN) and 99 non-exposed newborns (NEN) were compared for IL-6 and IL-10 levels. Sociodemographic and perinatal data, maternal psychopathology, consumption of nicotine and other substances were systematically collected in cases and controls. RESULTS: After adjusting for potential confounders, mean IL-6 was significantly higher in EN than in NEN (10,208.54, 95% confidence interval [95%CI] 1,328.54-19,088.55 vs. 2,323.03, 95%CI 1,484.64-3,161.21; p = 0.007; generalized linear model [GLM]). Mean IL-10 was also significantly higher in EN than in NEN (432.22, 95%CI 51.44-812.88 vs. 75.52, 95%CI 5.64-145.39, p = 0.014; GLM). Adjusted postpartum measures of IL-6 were significantly higher in mothers with a history of crack/cocaine use (25,160.05, 95%CI 10,958.15-39,361.99 vs. 8,902.14, 95%CI 5,774.97-12,029.32; p = 0.007; GLM), with no significant differences for IL-10. There was no correlation between maternal and neonatal cytokine levels (Spearman test, p ≥ 0.28 for all measures). CONCLUSIONS: IL-6 and IL-10 might be early biomarkers of PCE in newborns. These findings could help to elucidate neurobiological pathways underlying neurodevelopmental changes and broaden the range of possibilities for early intervention.

IL-6 and IL-10 levels in the umbilical cord blood of newborns with a history of crack/cocaine exposure in utero: a comparative study / Níveis de IL-6 e IL-10 no sangue de cordão umbilical de recém-nascidos com história de exposição intrauterina ao crack/cocaína: um estudo comparativo

Introduction Prenatal cocaine exposure (PCE) is associated with neurobehavioral problems during childhood and adolescence. Early activation of the inflammatory response may contribute to such changes. Our aim was to compare inflammatory markers (IL-6 and IL-10) both in umbilical cord blood and in maternal peripheral blood at delivery between newborns with history of crack/cocaine exposure in utero and non-exposed newborns. Methods In this cross-sectional study, 57 newborns with a history of crack/cocaine exposure in utero (EN) and 99 non-exposed newborns (NEN) were compared for IL-6 and IL-10 levels. Sociodemographic and perinatal data, maternal psychopathology, consumption of nicotine and other substances were systematically collected in cases and controls. Results After adjusting for potential confounders, mean IL-6 was significantly higher in EN than in NEN (10,208.54, 95% confidence interval [95%CI] 1,328.54-19,088.55 vs. 2,323.03, 95%CI 1,484.64-3,161.21; p = 0.007; generalized linear model [GLM]). Mean IL-10 was also significantly higher in EN than in NEN (432.22, 95%CI 51.44-812.88 vs. 75.52, 95%CI 5.64-145.39, p = 0.014; GLM). Adjusted postpartum measures of IL-6 were significantly higher in mothers with a history of crack/cocaine use (25,160.05, 95%CI 10,958.15-39,361.99 vs. 8,902.14, 95%CI 5,774.97-12,029.32; p = 0.007; GLM), with no significant differences for IL-10. There was no correlation between maternal and neonatal cytokine levels (Spearman test, p ≥ 0.28 for all measures). Conclusions IL-6 and IL-10 might be early biomarkers of PCE in newborns. These findings could help to elucidate neurobiological pathways underlying neurodevelopmental changes and broaden the range of possibilities for early intervention.

Introdução A exposição pré-natal à cocaína está associada a problemas neurocomportamentais durante a infância e adolescência. A ativação precoce da resposta inflamatória pode contribuir para tais alterações. Nosso objetivo foi comparar marcadores inflamatórios (IL-6 e IL-10) no sangue do cordão umbilical e no sangue periférico materno na hora do parto, entre recém-nascidos expostos ao crack intraútero e recém-nascidos não expostos. Métodos Neste estudo transversal, 57 recém-nascidos expostos ao crack intraútero (RNE) e 99 recém-nascidos não expostos (RNNE) foram comparados quanto aos níveis de IL-6 e IL-10. Dados sociodemográficos e perinatais, psicopatologia materna, consumo de nicotina e outras substâncias foram sistematicamente coletados em casos e controles. Resultados Após o ajuste para potenciais confundidores, a média de IL-6 foi significativamente maior nos RNE em comparação aos RNNE [10.208,54, intervalo de confiança (IC95%) 1.328,54-19.088,55 versus2.323,03, IC95% 1.484,64-3.161,21; p = 0,007; modelo linear generalizado (MLG)]. A média ajustada de IL-10 foi significativamente maior nos RNE do que nos RNNE (432,2189, IC95% 51,44-812,88 versus 75,52, IC95% 5,64-145,39, p = 0,014; MLG). Medidas pós-parto ajustadas de IL-6 foram significativamente maiores nas mães que usaram de crack/cocaína (25.160,05, IC95% 10.958,15-39.361,99 versus 8.902,14, IC95% 5.774,97-12.029,32; p = 0,007; MLG), sem diferenças significativas para IL-10. Não houve correlação entre níveis maternos e neonatais de citocinas (teste de Spearman, p ≥ 0,28 para todas as medidas). Conclusões IL-6 e IL-10 podem ser biomarcadores precoces da exposição pré-natal a cocaína em recém-nascidos. Esses resultados podem ajudar a elucidar as vias neurobiológicas subjacentes a alterações do desenvolvimento e aumentar a gama de possibilidades para intervenção precoce.

Socio-demographic and clinical characteristics of pregnant and puerperal crack-cocaine using women: preliminary data

BACKGROUND The literature provides several studies on the effects of cocaine when exposed to the fetus. However, the majority of these data comes from animal models. OBJECTIVE The objective of this study is to present socio-demographic and clinical data in crack-cocaine using pregnant women and their babies, as compared to non-users. METHODS Cross-sectional study, comprised by 56 dyads of crack-cocaine using mothers-babies and 89 control dyads. In addition to the socio-demographic data and the babies’ information, data collection was based on ABIPEMI for socioeconomic level, WAIS for IQ, MINI for psychopathology and ASSIST for drug use. RESULTS Most crack users, in comparison to non-users, did not have a partner (10.52% vs 4.4%, P = 0.001) and presented lower IQ (78.15, +/-8.07 vs 84.27 +/- 9.87; P = 0.002). The prevalence of antisocial personality disorder and suicide risk in users was higher than in non-users (24.44% vs none, P < 0.001; 28.26% vs 10.46% P = 0.01). Most of the users did not participate in prenatal care (75%). The babies that the crack-cocaine using mothers gave birth to weighed significantly less than the controls (2.858 g vs 3.240 g, P = 0.002). DISCUSSION Users had a higher degree of psychopathology and lower attendance in prenatal care. There was an overlap of adverse factors, both for exposed mothers and babies. The sum of these vulnerabilities could result in significant harm to the developing infant. .

Abordagem terapêutica de puérpera usuária de crack / Therapeutic approach of a crack-user puerperal woman

Este é o relato do atendimento de uma das mães dependentes do crack e de seu bebê, que frequentam o Ambulatório de Psiquiatria da Infância e da Adolescência do HCPA. Esta específica abordagem terapêutica visa atender a gestante e o bebê, ainda antes do nascimento, a fim de preservar um de seus direitos básicos, que é o de não ser intoxicado tão prematuramente.(AU)

This is the report of the treatment of one of the crack-dependent mothers and her baby who attended the Childhood and Adolescence Psychiatry outpatient clinic of the HCPA. This specific approach aims at attending the mother and the baby, even before birth, in order to preserve one of his/her basic rights, which is: not be intoxicated so prematurely.(AU)

O brincar na psicoterapia de orientação psicanalítica e alguns dos seus fundamentos teóricos básicos / Playing in psychoanalytic psychotherapy and some of its basic theorical fundamentals

A psiquiatria de crianças e adolescentes, tal como existe hoje, abrangendo inúmeras modalidades terapêuticas e contribuindo com várias áreas do conhecimento e da compreensão do desenvolvimento humano, teve como célula mãe, sem dúvida, a psicanálise. Desde o início do século XX gradativamente o brincar foi reconhecido, como uma via de acesso à dinâmica dos pensamentos, desejos e conflitos inconscientes das crianças, uma forma pela qual ela pode experimentar e elaborar de forma ativa as experiências traumáticas. A partir de conceitos psicanalíticos, os autores propõem o estudo da compreensão do brincar, essencial para a psicoterapia psicanalítica aplicada à criança e sua evolução. Apresentam e discutem aspectos históricos da psicanálise infantil e vislumbram um futuro promissor para o conhecimento da mente humana, enfocando os primórdios da relação mãe-bebê e sua aplicação à terapia de crianças, iluminando o campo da prevenção e benefeciando a saúde mental.(AU)

Children and adolescent psychiatry, as it exists today, including many therapeutic modalities and contributing to several areas of knowledge and understanding of human development, had its mother cell, the psychoanalysis. Since the beginning of the 20th century, children play has been gradually recognized as an access route to the dynamics of thoughts, desires, and unconsciuos conflicts of the child as a way in which they can experiment and develop proactively traumatic experiences. Based on psychoanalytic concepts, the authors propose the study of the meaning of play, which is essential to psychoanalyti psychotherapy, applied to children and their development. They present and discuss the historical aspects of child psychoanalysis and envision a bright future for the knowledge of the human mind, focusing on the origins of mother-infant relationship and its application to child therapy enlightening the prevention and mental health fields.(AU)

O brincar na psicoterapia de orientação psicanalítica e alguns dos seus fundamentos teóricos básicos / Playing in psychoanalytic psychotherapy and some of its basic theorical fundamentals

A psiquiatria de crianças e adolescentes, tal como existe hoje, abrangendo inúmeras modalidades terapêuticas e contribuindo com várias áreas do conhecimento e da compreensão do desenvolvimento humano, teve como célula mãe, sem dúvida, a psicanálise. Desde o início do século XX gradativamente o brincar foi reconhecido, como uma via de acesso à dinâmica dos pensamentos, desejos e conflitos inconscientes das crianças, uma forma pela qual ela pode experimentar e elaborar de forma ativa as experiências traumáticas. A partir de conceitos psicanalíticos, os autores propõem o estudo da compreensão do brincar, essencial para a psicoterapia psicanalítica aplicada à criança e sua evolução. Apresentam e discutem aspectos históricos da psicanálise infantil e vislumbram um futuro promissor para o conhecimento da mente humana, enfocando os primórdios da relação mãe-bebê e sua aplicação à terapia de crianças, iluminando o campo da prevenção e benefeciando a saúde mental.

Children and adolescent psychiatry, as it exists today, including many therapeutic modalities and contributing to several areas of knowledge and understanding of human development, had its mother cell, the psychoanalysis. Since the beginning of the 20th century, children play has been gradually recognized as an access route to the dynamics of thoughts, desires, and unconsciuos conflicts of the child as a way in which they can experiment and develop proactively traumatic experiences. Based on psychoanalytic concepts, the authors propose the study of the meaning of play, which is essential to psychoanalyti psychotherapy, applied to children and their development. They present and discuss the historical aspects of child psychoanalysis and envision a bright future for the knowledge of the human mind, focusing on the origins of mother-infant relationship and its application to child therapy enlightening the prevention and mental health fields.

Mental states as part of countertransference responses in psychotherapists facing reports of traumatic events of mourning and sexual violence.

The study aims to compare the mental states and countertransference responses of 92 psychodynamically oriented psychotherapists, male and female, experienced and inexperienced, facing written reports of real patients who experienced traumatic events. Two vignettes were presented: one of a sexual violence, the other the sudden death of a significant person. The Mental States Rating System (MSRS; Bouchard, Picard, Audet, Brisson, & Carrier, 1998), the MSRS Self-Report (Goldfeld & Bouchard, 2004), and the Inventory of Countertransference Behavior (ICB; Friedman & Gelso, 2000) were used. Results showed that the mourning vignette led to more reflective responses (MSRS) and the rape case was associated with more negative countertransference reactions (ICB). Female participants were more reflective (MSRS); male therapists used less mentalized states (MSRS Self-Report) and expressed more negative reactions (ICB) for both scenarios. Experienced therapists showed more positive reactions on the ICB. The construct validity of the instruments is discussed in relation to the findings.

Adaptação transcultural do Mental States Rating System para o português brasileiro / Cross-cultural adaptation of the Mental States Rating System to Brazilian Portuguese

INTRODUÇÃO: O artigo apresenta a adaptação transcultural do Mental States Rating System, uma escala de análise de conteúdo do discurso, seja ele falado, descrito ou filmado, que abrange de modo amplo tipos de contratransferência. MÉTODO: Foram realizadas as etapas de equivalência conceitual, equivalência de itens, equivalência semântica, equivalência operacional, equivalência funcional e aprovação da versão final pelo autor original do instrumento. RESULTADOS: Os critérios de equivalência foram satisfeitos, tendo a versão final sido aprovada pelo autor do instrumento original. CONCLUSÃO: A adaptação do Mental States Rating System disponibiliza para uso um instrumento que não só abrange todas as categorias de contratransferência descritas na literatura, mas as amplia, através da Teoria dos Estados Mentais. Constitui, desse modo, uma ferramenta de grande utilidade para pesquisa em psicoterapia e psicanálise, onde a contratransferência tem se mostrado um importante recurso, tornando-se ainda mais crucial nas patologias fundamentadas em estágios precoces do desenvolvimento, nos casos graves e nos traumas severos.

INTRODUCTION: This article presents a cross-cultural adaptation of the Mental States Rating System, a content analysis scale applied to spoken, written or taped material, which covers a wide range of countertransference categories. METHOD: The following steps were performed: conceptual equivalence, item equivalence, semantic equivalence, operational equivalence, functional equivalence, and approval of the final version by the author of the original instrument. RESULTS: The study has reached the objectives of equivalence, and the final Brazilian Portuguese version has been approved by the original author. CONCLUSION: The study provides a Brazilian Portuguese version of an instrument that not only covers all the range of categories encompassed by countertransference described in the literature, but also expands it through the Mental States Theory. This represents a valuable tool for research on psychotherapy and psychoanalysis, where countertransference has proved to be an important resource, especially for the treatment of diseases based on early stages of development, for severe mental diseases and severe trauma.

Adaptação transcultural do Inventory of Countertransference Behavior (ICB) para o português brasileiro / Cross-cultural adaptation of the Inventory of Countertransference Behavior (ICB) into Brazilian Portuguese

OBJETIVO: O artigo apresenta a adaptação transcultural do Inventory of Countertransference Behavior (ICB) para o português brasileiro. O ICB constitui-se numa escala de 21 itens que busca acessar o comportamento contratransferencial. Esta escala, que deve ser preenchida pelo supervisor após a sessão de supervisão, abrange a contratransferência em suas categorias positiva e negativa. MÉTODO: Foram realizadas as etapas de equivalência conceitual, equivalência de itens, equivalência semântica, equivalência operacional, equivalência funcional e aprovação da versão final pelo autor original do instrumento. RESULTADOS: Os critérios de equivalência foram satisfeitos, tendo a versão final sido aprovada pelo autor do instrumento original. CONCLUSÃO: A adaptação do ICB disponibiliza para uso um instrumento de utilização prática, que abrange as categorias de contratransferência positiva e negativa. Constitui, deste modo, uma ferramenta de grande utilidade para a clínica, a supervisão e a pesquisa em psicoterapia e psicanálise, onde a contratransferência tem se mostrado um importante recurso, especialmente no tratamento de patologias fundamentadas em estágios precoces do desenvolvimento, nos casos graves e nos traumas severos.

OBJECTIVE: This article presents a cross-cultural adaptation of the Inventory of Countertransference Behavior into Brazilian Portuguese. The Inventory of Countertransference Behavior is a 21-item scale designed to assess countertransferential behavior. This scale, which should be completed by the supervisor after a supervised session, comprehends countertransference and its positive and negative categories. METHOD: The following steps were performed: conceptual equivalence, item equivalence, semantic equivalence, operational equivalence, functional equivalence, and approval of the final version by the author of the original instrument. RESULTS: The study reached the objectives of equivalence, and the final Brazilian Portuguese version was approved by the original author. CONCLUSION: This adaptation provides a Brazilian Portuguese version of a practical instrument to assess positive and negative countertransference. It represents a valuable instrument for therapists, supervisors and researchers on psychotherapy and psychoanalysis, where countertransference has proved to be an important resource, especially to the treatment of diseases based on early stages of development, as well as to severe cases and severe trauma.

Adult mood disorders and childhood psychological trauma.

OBJECTIVE: To evaluate the association between adult mood disorders and childhood psychological trauma in a developing country. METHOD: Adults with and without mood disorders were assessed in a case-control study using the Mini International Neuropsychiatric Interview. Assessment of childhood trauma included physical and sexual abuse, frequent exposure to violence, and parental loss. RESULTS: In two independent multivariate analyses, after adjusting for potential confounding factors, we found a higher odds ratio for frequent exposure to violence in the community (p = .037) and for physical abuse by parents or caregivers during childhood/adolescence (p = .012) in the group with mood disorders than in the control group. In secondary analyses splitting the mood disorder group in two subgroups (manic episode, and major depressive episodes/ dysthymia), only manic patients showed significantly higher rates of frequent exposure to violence in the community (p = 0.01) and physical abuse during childhood (p = 0.02) than did patients in the control group. In addition, maniac patients had significantly higher rates of sexual abuse than did controls (p = .03). CONCLUSIONS: Our findings document an association between violence during childhood and adult mood disorders, especially for manic patients, in a developing country.

Adult mood disorders and childhood psychological trauma / Transtornos do humor no adulto e trauma psicológico na infância

OBJECTIVE: To evaluate the association between adult mood disorders and childhood psychological trauma in a developing country. METHOD: Adults with and without mood disorders were assessed in a case-control study using the Mini International Neuropsychiatric Interview. Assessment of childhood trauma included physical and sexual abuse, frequent exposure to violence, and parental loss. RESULTS: In two independent multivariate analyses, after adjusting for potential confounding factors, we found a higher odds ratio for frequent exposure to violence in the community (p = .037) and for physical abuse by parents or caregivers during childhood/adolescence (p = .012) in the group with mood disorders than in the control group. In secondary analyses splitting the mood disorder group in two subgroups (manic episode, and major depressive episodes/ dysthymia), only manic patients showed significantly higher rates of frequent exposure to violence in the community (p = 0.01) and physical abuse during childhood (p = 0.02) than did patients in the control group. In addition, maniac patients had significantly higher rates of sexual abuse than did controls (p = .03). CONCLUSIONS: Our findings document an association between violence during childhood and adult mood disorders, especially for manic patients, in a developing country.

OBJETIVO: Avaliar a associação entre transtornos de humor no adulto e trauma psicológico na infância em um país em desenvolvimento. MÉTODO: Adultos com e sem transtorno de humor foram avaliados em um estudo de caso-controle utilizando a Mini Entrevista Neuropsiquiátrica Internacional. A avaliação de trauma infantil incluiu abuso físico e sexual, exposição freqüente à violência e perda dos pais. RESULTADOS: Em duas análises multivariadas independentes, após o ajuste para fatores potenciais de confusão, encontramos uma razão de chance mais alta de exposição freqüente à violência na comunidade (p = 0,037) e de abuso físico por pais ou cuidadores durante a infância/adolescência (p = 0,012) no grupo com transtorno de humor do que no grupo controle. Em análises secundárias, dividindo-se o grupo com transtorno de humor em dois subgrupos (episódio maníaco e episódios de depressão maior/ distimia), somente pacientes maníacos demonstraram maiores índices de exposição freqüente à violência na comunidade (p = 0,01) e abuso físico durante a infância (p = 0,02) do que os pacientes no grupo controle. Além disso, os pacientes com mania tinham índices significativamente mais altos de abuso sexual do que os controles (p = 0,03). CONCLUSÕES: Nossos achados documentam uma associação entre violência na infância e transtornos de humor na idade adulta, especialmente em pacientes com mania, em um país em desenvolvimento.

Equilibrio hidroeletrolitico em recem-nascidos com peso inferior a 1000 gramas. Avaliacao de um novo protocolo de hidratacao / Hydroeletrolitic equilibrium in infant newborn with weigth inferior the 1000 gramas. Evaluation of one new protocol of hidration

Revisamos os prontuarios de 28 recem-nascidos com peso inferior a 1000 gramas a fim de determinar a eficacia de um novo protocolo para o manejo hidroeletrolitico. Quatorze recem-natos, admitidos antes da adocao do protocolo, formam o grupo I, enquanto os outro quatorze, admitidos apos a adocao doprotocolo, formam o grupo II. O peso medio de admnissao foi de 831+-127 gramas para o grupo II (p>0.05). Os grupos tambem eram comparaveis em relacao ao indicede Apgar, idade gestacional e uso de fototerapia ou berco aquecido. O volume hidrico administrado aos recem-nascidos do grupo II foi significantemente maior do que aos do grupo I, tanto no 2o. dia de vida (94+-25ml/kg/dia vs 130+-31 ml/kg/dia, p < 0.005) quanto no 4o. (117+-21 ml/kg/dia vs 176+-41 ml/kg/dia. p < 0.05).A taxa de sodio administrada tambem foi maior no grupo II no 2o. (1.0+-1.3mEq/kg/dia vs 1.6+-1.0mEq/kg/dia, p < 0.05) e 4o. dias de vida (2.6+-1.5mEq/kg/dia vs 4.7+-2.6mEq/kg/dia,p < 0.05). A perda do peso, por sua vez, foi menor nos recem-natos do grupo II (17.0+-5.0% vs 11.5+-5.0%, p <0.05). O mesmo ocorreu com o tempo necessario para a recuperacao do peso de nascimento (16.0+-4.8 dias vs 12.0+-2.9 dias, p <0.05). Nao observamos diferencas significativas na incidencia de Persistencia do Canal Arterial, Displasia Broncopulmonar, Hemorragia Intracranianae Disturbios Metabolicos, bem como na mortalidade entre os dois grupos. Concluimos que a adocao do novo protocolo permitiu um manejo mais dinamico do equilibriohidroeletrolitico, atenuando a perda ponderal observada nos primeiros dias de vida, sem aumento na incidencia de complicacoes .