Análisis de datos intra y postquirúrgicos en exodoncias unitarias realizadas por alumnos de grado de la FOUBA / Analysis of intra and post-surgical data in single extractions performed by FOUBA undergraduate studens

La exodoncia es el procedimiento odontológico más antiguo del que se tiene registro, pero pocas publi-caciones abarcan con detalle esta temática. Conocer los pormenores de esta práctica puede servir para generar políticas educativas, sanitarias, como así también sistematizarla y bajar así sus riesgos y complicaciones. El objetivo del presente estudio fue describir y analizar variables quirúrgicas asocia-das a las extracciones unitarias de piezas dentarias. Los datos se analizaron mediante las pruebas Chi-cuadrado de Pearson, exacta de Fisher y Kruskal-Wallis, según lo que correspondía (p<0,05, signifi-cativo). Concurrieron más mujeres que hombres, con una mediana de edad de 37 años (intervalo, 18 a 86), siendo los terceros molares las piezas más ex-traídas. La pieza que requirió más odontosecciones fue el primer molar superior, mientras que la pieza que requirió más alveolectomías fue el tercer mo-lar inferior, siendo esta última la pieza con mayores complicaciones intra y post quirúrgicas, incluso una alteración nerviosa. La caries penetrante fue amplia-mente el motivo más frecuente de exodoncias (79%) superando los reportes en estudios similares, La du-ración promedio (DE) de las extracciones unitarias fue de 39 minutos (21), pero difirió significativamente entre piezas dentarias (p<0,05). Las complicaciones post quirúrgicas se asociaron significativamente a cirugías más prolongadas (p<0,05). La cantidad de anestubos utilizados también difirió significativa-mente entre piezas dentarias (p<0,05), siendo el sec-tor posterior inferior el que más cantidad necesitó. Los datos aportados en el estudio pueden ser utiliza-dos para mejorar recursos en los servicios de salud odontológicos (AU)

Dental extractions are the first procedures reported in dentistry, but few articles focus on its individual details. With proper information, educational and health policies could be systematically improved, and thus reduce risks and complications. The aim of the study was to describe and analyze surgical variables associated with single tooth extractions performed by students. The practice of 500 single extractions on 500 patients who attended the Oral and Maxillofacial service of the School of Dentistry of the University of Buenos Aires, between September 2021 and September 2022, performed by fourth-year students supervised by teachers, are described. Data were analyzed using Pearson's Chi-square, Fisher's exact or Kruskal-Wallis tests, as appropriate (p<0.05, significant). More women attended than men, with a median age of 37 years (range 18 to 86), with third molars being the most extracted pieces. Decay teeth was by far the most frequent reason for extractions (79%), exceeding reports in similar studies, that may be explained by a younger sample and the multiple extractions exclusion. The tooth that required the most sections was the upper first molar, while the tooth that required the most alveolectomies was the lower third molar, the latter being the tooth with the greatest intra- and post-surgical complications, including a reported nerve damage. The average duration (SD) of single extractions was 39 minutes (21), but it differed significantly between teeth (p<0.05), for example, upper central incisors presented an average of 21 (9), and upper first premolars 47 (25), characteristics not reported to date. As other studies reported, post-surgical complications were significantly associated with longer surgeries (p<0.05). The amount of anesthesia cartridges used also differed significantly between teeth (p<0.05), being the posterior mandible the one that needed the most amount. The data provided in the study can be systematically used to improve temporal and economic resources in dental health services (AU)

El Miedo en Relación a la Odontología Uso de la Escala del Miedo Dental / Fear in Relation to Dentistry Using the Dental Fear Scale

La atención odontológica despierta en numerosos pacientes, especialmente en los niños, miedo y ansiedad que pueden originar conductas de rechazo a la misma o dificultar el trabajo del profesional. Para registrar la prevalencia e identificar los miedos más frecuentes en relación a la atención odontológica, se realizó un estudio observacional, descriptivo y longitudinal en un trabajo interdisciplinario con Musicoterapia. Se utilizó la Escala del Miedo (CFSS-DS), Dental Subscale of the Childrens' Fear Survey. Los resultados en un promedio de edades en años 11,41; desvío = 2,80, registraron el 53% en la puntuación 38:39. Corresponde 54.34% al sexo femenino y 45.66% al sexo masculino. El mayor porcentaje de miedo se registró a los 10 años de edad 15.24% y en el sexo femenino 54.34%. En conclusión, registrar y reconocer los miedos dentales ofreció un espacio de reflexión y elaboración de los mismos, con docentes y alumnos de grado, para desarrollar, en un tratamiento interdisciplinario con musicoterapia, estrategias que favorezcan una mejor adherencia de los pacientes al tratamiento odontológico y habilidades inherentes y habilidades sociales inherentes a la relación odontólogo-paciente. El miedo manifiesto a la atención odontológica sin ser resuelto satisfactoriamente puede entorpecer las mejores intenciones del profesional en el desarrollo de su tarea (AU)

Estudio comparativo de las complicaciones quirúrgicas de los alumnos de la asignatura Cirugía I, entre exodoncias del tercer molar inferior y el resto de las piezas dentarias / A comparative study of surgical complications of the students at the Surgery I Department, between lower third molar extractions and the rest of the dental piecesns

La extracción dental es una operación delicada que requiere de odontólogos capacitados y entrenados para ejecutarla adecuadamente. El tercer molar inferior es una pieza dentaria que suma dificultades para su exodoncia debido a una serie de particularidades: puede encontrarse con diferentes niveles de erupción y posiciones; presenta el mayor porcentaje de desviaciones linguales o bucales; su atipia radicular es frecuente; se encuentra ubicado en la posición más posterior de la arcada, a veces con carrillos o lenguas que dificultan su visibilidad y maniobras; presenta un hueso reforzado de máxima resistencia y muy poca elasticidad; se encuentra muy cercano a reparos anatómicos de cuidado como el conducto mandibular y la zona de hueso lingual. A mayor dificultad de la cirugía, mayores posibilidades de complicaciones. Se detalla un trabajo que describe las complicaciones quirúrgicas intra y posoperatorias durante la práctica de exodoncias, entre el tercer molar inferior y el resto de las piezas dentarias, hecha en la Clínica de Grado de la Cátedra de Cirugía I, FOUBA (AU)

Short communication: Cold atmospheric plasma inactivation of Prototheca zopfii isolated from bovine milk.

Mastitis is a serious bovine diseases that can be caused by Prototheca zopfii, yeast-like algae belonging to the family Chlorellaceae. The substantial economic losses and health damage associated with bovine mastitis emphasize the need to develop effective strategies aimed at control of the infection. Unfortunately, P. zopfii is highly resistant to most common antibacterial and antifungal agents, as well as to heat treatment. We report here the first attempt to use cold atmospheric plasma to inactivate this pathogen. We studied 20 strains of P. zopfii isolated from milk samples taken from cows with clinical or subclinical mastitis. The studies confirmed the high level of resistance of P. zopfii to typical antifungal agents, such as voriconazole, fluconazole, amphotericin B, caspofungin, anidulafungin, and micafungin. In contrast, each of the strains revealed high susceptibility to cold atmospheric plasma, >2-fold higher compared with a reference strain of Candida albicans. The obtained results are promising and open up a new approach in the fight against P. zopfii.

Estudio retrospectivo de una población de pacientes sometidos a exodoncias en la Cátedra de Cirugía y Traumatología Bucomaxilofacial I de la Facultad de Odontología de la Universidad de Buenos Aires, en el período entre enero 2012 - mayo 2014 / A retrospective study on a patient population undergoing tooth extraction at the Department of Oral and Maxillofacial Surgery I, School of Dentistry, University of Buenos Aires, over a January 2012 - May 2014 period

La extracción dentaria es una de las prácticas odontológicas realizadas con mayor frecuencia, pero a corto plazo los pacientes necesitan rehabilitaciones orales para optimizar el funcionamiento del sistema estomatognático. Dado que conocer las características de la población que demanda exodoncias puede contribuir a elaborar estrategias para limitar la pérdida de piezas dentarias, nos propusimos 1) registrar las características de la población que demanda exodoncias; 2) identificar las piezas dentarias extraídas; 3) registrar las causas de exodoncia; 4) identificar patologías bucales durante la consulta para una exodoncia. Para llevarlo a cabo se seleccionaron aleatoriamente 2000 historias clínicas de pacientes que concurrieron a la Cátedra de Cirugía y Traumatología Buco Maxilofacial I de la Facultad de Odontología de la Universidad de Buenos Aires (FOUBA) y se realizaron exodoncias con anestesia local entre enero de 2012 y mayo de 2014...

Dental extraction is one of the most frequent practices in dentistry, causing necessary oral rehabilitation to ensure normal stomatognathic system function. The purpose of this study was to get to know the characteristics of the population in need of dental extraction so as to develop strategies tending to limit teeth loss. We set to 1) record the characteristics of the population on demand for dental extraction; 2) Identify teeth extracted; 3) Record cause of dental extraction; 4) Identify oral pathologies during examination for dental extraction. We selected 2000 Records of patients who attended “Cátedra de Cirugía y Traumatología Bucomaxilofacial I de la Facultad de Odontología de la Universidad de Buenos Aires, FOUBA” for dental extraction with local anesthetics in the period between January 2002 and May 2014...

New insights into host-pathogen interactions during Entamoeba histolytica liver infection.

Amoebiasis is the third worldwide disease due to a parasite. The causative agent of this disease, the unicellular eukaryote Entamoeba histolytica, causes dysentery and liver abscesses associated with inflammation and human cell death. During liver invasion, before entering the parenchyma, E. histolytica trophozoites are in contact with liver sinusoidal endothelial cells (LSEC). We present data characterizing human LSEC responses to interaction with E. histolytica and identifying amoebic factors involved in the process of cell death in this cell culture model potentially relevant for early steps of hepatic amoebiasis. E. histolytica interferes with host cell adhesion signalling and leads to diminished adhesion and target cell death. Contact with parasites induces disruption of actin stress fibers and focal adhesion complexes. We conclude that interference with LSEC signalling may result from amoeba-triggered changes in the mechanical forces in the vicinity of cells in contact with parasites, sensed and transmitted by focal adhesion complexes. The study highlights for the first time the potential role in the onset of hepatic amoebiasis of the loss of liver endothelium integrity by disturbance of focal adhesion function and adhesion signalling. Among the amoebic factors required for changed LSEC adherence properties we identified the Gal/GalNAC lectin, cysteine proteases and KERP1.

Analysis of the diffusion weighted MR microscopy data of excised spinal cord of a rat on the basis of the model of restricted diffusion.

Anisotropic diffusion in the excised rat spinal cord saturated with 0.9% saline was investigated using MR microimaging with b-values up to 8000 s/mm2 for different diffusion times. Non-exponential transversal diffusion decay found in white matter (WM) and gray matter (GM) was fitted with 2 components (the "fast" and the "slow"). Significantly smaller non-exponential dependence was found for the longitudinal diffusion in the WM. Obtained results corresponding to restricted diffusion in the range from approximately 2 to approximately 7 microm were correlated with axon diameter distribution in the WM obtained from transmission electron micrographs. It was concluded that observed diffusion anisotropy in the spinal cord might be entirely explained by presence of the slow transversal component, arising from the restricted diffusion. The strict analytical description of the diffusion decay in nervous tissue requires taking into account continuous distribution of the space-scale of the restricting barriers. The simplified two-component analysis may be applicable for visualization of the nervous tissue in clinical practice.

Different extent of inhibition of pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase both containing endogenous thiamine pyrophosphate, by some anticoenzyme analogues.

Inhibitory effects of 4'-oxythiamine pyrophosphate (OTPP) and tetrahydrothiamine pyrophosphate (ThTPP) on the purified bison heart pyruvate dehydrogenase complex (PDC) semisaturated with endogenous thiamine pyrophosphate (TPP) and 2-oxoglutarate dehydrogenase complex (OGDC) saturated about 85% with endogenous TPP, were studied. It has been established that the thiamine derivatives strongly inhibit not only the PDC apoenzyme moiety, but also the PDC holoenzyme moiety. The apparent I50 values for the holoenzyme were 0.006 microM and 0.046 microM for OTPP and ThTPP, respectively. The inhibition of the PDC is reversible. After removal of the anticoenzyme analogues by gel filtration the endogenous TPP within the PDC is retained as it is evidenced by complete recovery of the enzyme activity without added TPP. In contrast with the PDC, OGDC holoenzyme form is weakly inhibited by the anticoenzyme analogues.

Thiamine pyrophosphate as an effector of 2-oxoglutarate dehydrogenase complex from European bison heart.

The purified 2-oxoglutarate dehydrogenase complex (OGDC) from the European bison heart was near saturated with endogenous bound thiamine pyrophosphate (TPP). Exogenous TPP added to the full OGDC reaction medium decreased S0.5 for 2-oxoglutarate approximately 2.6-fold without any notable change in the maximum reaction rate. The TPP effect was observed in the presence of 1 mM ADP which alone is a strong positive allosteric effector of OGDC. At an unsaturating 2-oxoglutarate concentration the A50 value for TPP was approximately 0.05 mM. The ADP-like action of exogenous TPP was also found in the 2-oxoglutarate dehydrogenase (E1) reaction, determined in the presence of 2,6-dichlorophenoloindophenol as an electron acceptor.

Some regulatory properties of the 2-oxoglutarate dehydrogenase complex from European bison heart.

Basic regulatory properties of the 2-oxoglutarate dehydrogenase complex (OGDC) isolated and purified from the heart muscle of European bison (Bison bonasus) were studied. Kinetic studies have shown that in the absence of phosphate ions OGDC exhibits kinetic attributes of negative cooperativity with respect to 2-oxoglutarate. ADP and phosphate lower S0.5 value of OGDC for 2-oxoglutarate without changing the maximum reaction rate. NADH inhibits OGDC versus both 2-oxoglutarate and NAD+. Moreover, bison heart OGDC shows negative kinetic cooperativity for NAD+ and positive kinetic cooperativity for CoA at low CoA concentrations. The latter property has not been observed in earlier studies on OGDC from bovine and pig heart and other tissues of these animals.

[Analysis of risk factors and prevalence of spinal osteoporosis in a population sample of Warsaw residents over 50 years of age]. / Analiza czynników ryzyka i czestosc wystepowania osteoporozy kregoslupa w próbce populacyjnej mieszkanców Warszawy powyzej 50. roku zycia.

Vertebral fractures are the one typically seen in osteoporosis. An incidence of vertebral body fractures and risk factors for osteoporosis were analysed in the retrospective population based epidemiological study. Population sample included 607 Warsaw inhabitants aged between 50 and 80 years (301 women and 306 men divided into age groups of 5 years, including approx. 50 subjects each). Vertebral body fractures were diagnosed on thoraco-lumbar spine X-rays made in the lateral projection. Risk factors for osteoporosis were analysed with the aid of an questionnaire. Bone mineral density was measured with dual energy X-ray absorptiometry and single photon absorptiometry in about 1/3 of the examined women. The prevalence of vertebral osteoporosis was high in both women (20.5%) and in men (27.8%). Low dietary calcium intake (below 800 mg daily) and low serum vitamin D levels (250HD less than 11 micrograms/ml) were characteristic for about 90% of the examined population. In osteoporotic subjects the following risk factors were significantly more frequent: age (p < 0.01), tobacco smoking (p < 0.001), low physical activity (p < 0.05), long-term immobilization (p < 0.01). Densitometry showed significantly lower bone mineral density in both women with vertebral fractures (p < 0.01) and low serum vitamin D levels (p < 0.05). Osteoporotic vertebral fractures were diagnosed more frequently in women with early menopause (p < 0.001). In the examined population there is a ligh prevalence of vertebral osteoporosis (predominating in mean) and risk factors.

[Eosinophilic non-allergic rhinitis. Clinical and cytological evaluation]. / Eozynofilowy niealergiczny niezyt nosa. Ocena kliniczna i cytologiczna.

In 29 patients with nasal obstruction, sneezing and water-like nasal discharge the allergic tests were negative. However, the cytologic examinations of their nasal discharge founded 20% of eosinophil The IgE serum level was normal in 21 patients and in 8 was distinctly elevated. In 21 patients the non-allergic rhinitis was diagnosed and in remaining 8 the suspicion of allergy without proved agent.

[Results of the treatment of neurosyphilis with high doses of crystalline and procaine penicillins]. / Wyniki leczenia kily ukladu nerwowego wysokimi dawkami penicyliny krystalicznej i prokainowej.

The results of the treatment in two groups of patients with neurosyphilis are compared. In 23 cases, i.v. infusions of crystalline penicillin were given for 10 days in daily dose of 20-24 mega U., combined with probenecid. Then it was followed by i.m. procaine penicillin in daily dose of 1.2 mega U., in total dose 12-50 mega U. The mean duration of follow-up was 7,8 months. The second group (34 cases) was treated with procaine penicillin i.m. in daily dose of 1.2 mega U., the total dose was 24-60 mega U. The mean duration of follow-up was 2.5 years. The differences between the results of the treatment in both groups were not significant. In 3 reinfected patients with early asymptomatic neurosyphilis in spite of intensive treatment with penicillin sero- and cerebro-spinal fluid-resistant syphilis developed.

[Double skin cancer coexisting with chronic myeloid leukemia]. / Dwukrotny rak skóry i przewlekla bialaczka szpikowa.

In a 71-year-old man chronic myeloid leukaemia was diagnosed 4--5 years after cure of pulmonary tuberculosis and removed auricular cancer and 2 years after nasal cancer cured by means of local radiotherapy.