Correction: In-season internal and external training load quantification of an elite European soccer team.

[This corrects the article DOI: 10.1371/journal.pone.0209393.].

Changes in strength-related outcomes following velocity-monitored resistance training with 10 % and 20 % velocity loss in older adults.

We compared the effects of velocity-monitored resistance training with an intra-set velocity loss (i.e., the decrement in repetition velocity over the set) of 10 % vs. 20 % on strength-related outcomes in older adults. We randomly assigned eighteen older adults to a velocity loss group of 10 % (n = 10; 78 ± 12 years) or 20 % (n = 8; 73 ± 10 years) to perform a 10-week training program. The primary outcomes were the one-repetition maximum (1RM) and the average mean velocity against absolute loads associated with loads <60 % 1RM (MVlow) and ≥ 60 % 1RM (MVhigh) in the leg and chest press exercises, assessed at pre-, mid- (week 5), and post-test. Secondary outcomes included handgrip strength, 1-kg medicine ball throw distance, 10-m walking time, and five-repetition sit-to-stand time. No differences between groups were found in any outcome at any time (p > 0.05). Both groups improved the 1RM leg press from pre- to mid- and post-test and the MVlow and MVhigh from pre- to mid-test (p < 0.05). No group improved the 1RM chest press (p > 0.05), but both increased the MVlow from pre- to mid-test (p < 0.05). Furthermore, both groups improved the sit-to-stand time, while only the 20 % velocity loss group significantly improved handgrip strength and 10-m walking time (p < 0.05). The results showed that both velocity losses improved leg press strength and velocity, chest press velocity, and sit-to-stand time in older adults, although a 10 % velocity loss was more efficient as it required less volume (i.e., total repetitions) than 20 %. Nevertheless, the latter seems required to optimize handgrip strength and 10-m walking time in older people.

Nanotechnology-Based Topical Delivery of Natural Products for the Management of Atopic Dermatitis.

Atopic dermatitis (AD) is a chronic eczematous inflammatory disease that may arise from environmental, genetic, and immunological factors. Despite the efficacy of current treatment options such as corticosteroids, such approaches are mainly focused on symptom relief and may present certain undesirable side effects. In recent years, isolated natural compounds, oils, mixtures, and/or extracts have gained scientific attention because of their high efficiency and moderate to low toxicity. Despite their promising therapeutic effects, the applicability of such natural healthcare solutions is somewhat limited by their instability, poor solubility, and low bioavailability. Therefore, novel nanoformulation-based systems have been designed to overcome these limitations, thus enhancing the therapeutic potential, by promoting the capacity of these natural drugs to properly exert their action in AD-like skin lesions. To the best of our knowledge, this is the first literature review that has focused on summarizing recent nanoformulation-based solutions loaded with natural ingredients, specifically for the management of AD. We suggest that future studies should focus on robust clinical trials that may confirm the safety and effectiveness of such natural-based nanosystems, thus paving the way for more reliable AD treatments.

Delocalized electronic excitations and their role in directional charge transfer in the reaction center of Rhodobacter sphaeroides.

In purple bacteria, the fundamental charge-separation step that drives the conversion of radiation energy into chemical energy proceeds along one branch-the A branch-of a heterodimeric pigment-protein complex, the reaction center. Here, we use first principles time-dependent density functional theory (TDDFT) with an optimally-tuned range-separated hybrid functional to investigate the electronic and excited-state structure of the six primary pigments in the reaction center of Rhodobacter sphaeroides. By explicitly including amino-acid residues surrounding these six pigments in our TDDFT calculations, we systematically study the effect of the protein environment on energy and charge-transfer excitations. Our calculations show that a forward charge transfer into the A branch is significantly lower in energy than the first charge transfer into the B branch, in agreement with the unidirectional charge transfer observed experimentally. We further show that the inclusion of the protein environment redshifts this excitation significantly, allowing for energy transfer from the coupled Qx excitations. Through analysis of transition and difference densities, we demonstrate that most of the Q-band excitations are strongly delocalized over several pigments and that both their spatial delocalization and charge-transfer character determine how strongly affected they are by thermally-activated molecular vibrations. Our results suggest a mechanism for charge-transfer in this bacterial reaction center and pave the way for further first-principles investigations of the interplay between delocalized excited states, vibronic coupling, and the role of the protein environment in this and other complex light-harvesting systems.

Estimating the relative load from movement velocity in the seated chest press exercise in older adults.

AIM: This study aimed to i) determine the load-velocity relationship in the seated chest press in older adults, ii) compare the magnitude of the relationship between peak and mean velocity with the relative load, and iii) analyze the differences between sexes in movement velocity for each relative load in the chest press. MATERIAL AND METHODS: Thirty-two older adults (17 women and 15 men; 79.6±7.7 years) performed a chest press progressive loading test up to the one-repetition maximum (1RM). The fastest peak and mean velocity reached with each weight were analyzed. Quadratic equations were developed for both sexes and the effectiveness of the regression model was analyzed through a residual analysis. The equations were cross-validated, considering the holdout method. The independent samples t-test analyzed i) the differences in the magnitude of the relationship between peak and mean velocity with the relative load and ii) the differences between sexes in the peak and mean velocity for each relative load. RESULTS: It was possible to observe very strong quadratic load-velocity relationships in the seated chest press in women (peak velocity: r2 = 0.97, standard error of the estimate (SEE) = 4.5% 1RM; mean velocity: r2 = 0.96, SEE = 5.3% 1RM) and men (peak velocity: r2 = 0.98, SEE = 3.8% 1RM; mean velocity: r2 = 0.98, SEE = 3.8% 1RM) without differences (p>0.05) in the magnitude of the relationship between peak and mean velocity with the relative load. Furthermore, there was no overfitting in the regression models due to the high and positive correlation coefficients (r = 0.98-0.99). Finally, men presented higher (p<0.001) lifting velocities than women in almost all relative loads, except for 95-100% 1RM (p>0.05). CONCLUSION: Measuring repetition velocity during the seated chest press is an objective approach to estimating the relative load in older adults. Furthermore, given the velocity differences between older women and men at submaximal loads, it is recommended to use sex-specific equations to estimate and prescribe the relative loads in older adults.

Influence of the Competitive Level and Weight Class on Technical Performance and Physiological and Psychophysiological Responses during Simulated Mixed Martial Arts Fights: A Preliminary Study.

This study aimed to analyze the influence of the competitive level and weight class on technical performance and physiological and psychophysiological responses during simulated MMA fights. Twenty MMA male athletes were divided into four groups: heavyweight elite (HWE; n = 6), lightweight elite (LWE; n = 3), heavyweight professional (HWP; n = 4), and lightweight professional (LWP; n = 7). All athletes performed four simulated fights of three 5-min rounds with a 1-min rest interval. Each fight was recorded using a video camera to analyze offensive and defensive actions. Moreover, the following measures were made: heart rate (before and after each round), blood lactate concentration (before and after the fight), readiness state (before each round), and the rate of perceived exertion (RPE) (after each round). The main findings were: i) LWE athletes applied more offensive touches than LWP athletes; ii) HWP athletes presented higher heart rate values than LWP athletes after the first round; however, LWP athletes presented greater heart rate changes than HWP athletes from the first to the second round; iii) no differences existed between groups in blood lactate concentration and readiness state; and iv) HWP and LWP athletes presented higher RPE values than LWE athletes in the first and third rounds; however, LWE athletes presented greater RPE changes than HWE, HWP, and LWP athletes from the first to the second and third rounds. This study shows that LWE athletes apply more offensive touches than LWP athletes during simulated MMA fights. Moreover, lightweight athletes tend to increase their physiological demand as the combat evolves, which is also reflected in their RPE.

Exploring Iberian Peninsula Lamiaceae as Potential Therapeutic Approaches in Wound Healing.

Skin tissue has a crucial role in protecting the human body from external harmful agents, preventing wounds that frequently demand proper healing approaches. The ethnobotanical knowledge of specific regions with further investigation on their medicinal plants has been paramount to create new and effective therapeutical agents, including for dermatological purposes. This review attempts, for the first time, to investigate the traditional applications of Lamiaceae medicinal plants that are already used by local communities in the Iberian Peninsula in wound healing. Henceforward, Iberian ethnobotanical surveys were reviewed, and the information about the traditional wound healing practices of Lamiaceae was comprehensively summarized. Afterwards, the scientific validation of each Lamiaceae species was exhaustively checked. From this, eight out of twenty-nine Lamiaceae medicinal plants were highlighted by their wound-related pharmacological evidence and are in-depth presented in this review. We suggest that future studies should focus on the isolation and identification of the active molecules of these Lamiaceae, followed by robust clinical trials that may confirm the security and effectiveness of such natural-based approaches. This will in turn pave the way for more reliable wound healing treatments.

Essential Oils from Côa Valley Lamiaceae Species: Cytotoxicity and Antiproliferative Effect on Glioblastoma Cells.

Lavandula pedunculata (Mill.) Cav., Mentha cervina L. and Thymus mastichina (L.) L. subsp. mastichina are representative species of the Côa Valley's flora, a Portuguese UNESCO World Heritage Site. L. pedunculata and T. mastichina are traditionally used to preserve olives and to aromatize bonfires on Saint John's Eve, while M. cervina is mainly used as a spice for river fish dishes. Despite their traditional uses, these aromatic plants are still undervalued, and literature regarding their bioactivity, especially anticancer, is scarce. In this work, the morphology of secretory structures was assessed by scanning electron microscopy (SEM), and the composition of essential oils (EOs) was characterized by gas chromatography-mass spectrometry (GC-MS). The study proceeded with cytotoxic evaluation of EOs in tumor and non-tumor cells with the cell death mechanism explored in glioblastoma (GB) cells. L. pedunculata EO presented the most pronounced cytotoxic/antiproliferative activity against tumor cells, with moderate cytotoxicity against non-tumor cells. Whereas, M. cervina EO exhibited a slightly lower cytotoxic effect against tumor cells and did not affect the viability of non-tumor cells. Meanwhile, T. mastichina EO did not induce a strong cytotoxic effect against GB cells. L. pedunculata and M. cervina EOs lead to cell death by inducing apoptosis in a dose-dependent manner. The present study suggests that L. pedunculata and M. cervina EOs have a strong cytotoxic and antiproliferative potential to be further studied as efficient antitumor agents.

Manipulating the Resistance Training Volume in Middle-Aged and Older Adults: A Systematic Review with Meta-Analysis of the Effects on Muscle Strength and Size, Muscle Quality, and Functional Capacity.

BACKGROUND: Effective manipulation of the acute variables of resistance training is critical to optimizing muscle and functional adaptations in middle-aged and older adults. However, the ideal volume prescription (e.g., number of sets performed per exercise) in middle-aged and older adults remains inconclusive in the literature. OBJECTIVE: The effects of single versus multiple sets per exercise on muscle strength and size, muscle quality, and functional capacity in middle-aged and older adults were compared. Moreover, the effects of single versus multiple sets per exercise on muscular and functional gains were also examined, considering the influence of training duration. METHODS: Randomized controlled trials and non-randomized controlled trials comparing single versus multiple sets per exercise on muscle strength, muscle size, muscle quality, or functional capacity in middle-aged and older adults (aged ≥ 50 years) in the PubMed/MEDLINE, Web of Science, and Scopus databases (01/09/2021, updated on 15/05/2022) were identified. A random-effects meta-analysis was used. RESULTS: Fifteen studies were included (430 participants; 93% women; age 57.9-70.1 years). Multiple sets per exercise produced a greater effect than single sets on lower-limb strength (standardized mean difference [SMD] = 0.29; 95% confidence interval [CI] 0.07-0.51; mean difference [MD] = 1.91 kg; 95% CI 0.50-3.33) and muscle quality (SMD = 0.40; 95% CI 0.05-0.75) gains. There were no differences between single versus multiple sets per exercise for upper-limb strength (SMD = 0.13; 95% CI - 0.14 to 0.40; MD = 0.11 kg; 95% CI - 0.52 to 0.75), muscle size (SMD = 0.15; 95% CI - 0.07 to 0.37), and functional capacity (SMD = 0.01; 95% CI - 0.47 to 0.50) gains. In addition, there were no differences between single versus multiple sets on muscle strength and size gains for training durations ≤ 12 weeks or > 12 weeks. CONCLUSIONS: Multiple sets per exercise produced greater lower-limb strength and muscle quality gains than single sets in middle-aged and older adults, although the magnitude of the difference was small. In contrast, single sets per exercise were sufficient to improve upper-limb strength, muscle size, and functional capacity in these populations. Despite these findings, researchers should conduct future high-quality, pre-registered, and blinded randomized controlled trials to strengthen the scientific evidence on this topic.

Acute Effects of Heavy Strength Training on Mechanical, Hemodynamic, Metabolic, and Psychophysiological Parameters in Young Adult Males.

This study analyzed the acute effects of heavy strength training on mechanical, hemodynamic, metabolic, and psychophysiological responses in adult males. Thirteen recreational level males (23.3 ± 1.5 years) randomly performed two heavy strength training sessions (3 sets of 8 repetitions at 80% of one repetition maximum [1RM]) using the bench press (HST-BP) or full squat (HST-FS)). The repetition velocity was recorded in both sessions. Moreover, before and after the sessions, the velocity attained against the ~1.00 m·s−1 load (V1Load) in the HST-BP, countermovement jump (CMJ) height in the HST-FS, blood pressure, heart rate, blood lactate, and psychophysiological responses (OMNI Perceived Exertion Scale for Resistance Exercise) were measured. There were differences between exercises in the number of repetitions performed in the first and third sets (both <8 repetitions). The velocity loss was higher in the HST-BP than in the HST-FS (50.8 ± 10.0% vs. 30.7 ± 9.5%; p < 0.001). However, the mechanical fatigue (V1Load vs. CMJ height) and the psychophysiological response did not differ between sessions (p > 0.05). The HST-FS caused higher blood pressure and heart rate responses than the HST-BP (p < 0.001 and p = 0.02, respectively) and greater blood lactate changes from pre-training to post-set 1 (p < 0.05). These results showed that the number of maximal repetitions performed in both sessions was lower than the target number and decreased across sets. Moreover, the HST-BP caused a higher velocity loss than the HST-FS. Finally, the HST-FS elicited higher hemodynamic and metabolic demand than the HST-BP.

Strength and Power Performance Changes During an In-Season Resistance Training Program in Elite Futsal Players: A Case Study.

In this study, we aimed to analyze (i) the strength and power changes after resistance training (RT) in elite futsal players, and (ii) the associations between the session rate of perceived exertion (sRPE) and perceived total quality recovery (TQR), and the sRPE and TQR with the volume load of the RT program. Ten elite futsal players (24.8 ± 5.4 years; 76.2 ± 7.1 kg; 1.77 ± 0.05 m) performed an in-season 8-week RT program twice per week. RT consisted of 2-3 sets x 3-6 reps at 45-65% of one-repetition maximum (1RM) with maximal velocities in the full squat and complementary exercises with the same volume. We assessed the TQR before every session, while the sRPE was calculated after each RT session. One week before and after the intervention, we measured the countermovement jump (CMJ) height, isometric hip adduction strength (IHAS), 1RM, and peak power (PP) in the full squat progressive loading test. After the 8-week training program, there was a significant improvement in most outcomes, yet the gains (%Δ) remained below the minimal detectable change (MDC), except for IHAS (CMJ: p < 0.05, %Δ = 6.7, MDC% = 7.2; IHAS: p < 0.001, %Δ = 19.1, MDC% = 14.6; 1RM: p > 0.05, %Δ = 9.2, MDC% = 21.5; PP: p < 0.05; %Δ = 14.4, MDC% = 22.4). We also found a significant negative correlation between TQR and the sRPE (r = -0.45, p < 0.001). Our data suggest that RT based on low-volume and low-to-moderate loads may not produce a sufficient stimulus to induce meaningful dynamic strength and power gains in elite futsal players, although it improves isometric strength. Furthermore, monitoring TQR before sessions may show coaches how the elite futsal player will perceive the session's intensity.

Validity and Reliability of The Smart Groin Trainer for Measuring Hip Adduction Strength.

We analyzed the Smart Groin Trainer device's validity and reliability to measure the isometric hip adduction strength during the adductor squeeze strength test. Fifteen professional soccer players (25.33±4.06 years) and fifteen university students (21.60±1.76 years) participated in this study. All participants performed the squeeze strength test using two portable dynamometers: Smart Groin Trainer and Globus Ergometer. Three maximal isometric hip adduction contractions lasting 5s, interspersed by 3min rest intervals, were performed. Reliability was analyzed with intraclass correlation coefficients, standard error of measurements, and minimal detectable change. The absolute percent error and inter-device accuracy were also analyzed. Correlation analysis assessed the inter-device concurrent validity. The results found no significant differences (p>0.05) between devices in the squeeze strength test values in soccer players and university students. Inter-device comparisons revealed excellent levels of reliability and accuracy in soccer players. Concurrent validity measures revealed strong inter-device relationships in soccer players (r=0.89) and very strong relationships in university students (r=0.99). Intra-device analysis using the Smart Groin Trainer showed excellent relative and absolute reliability in tested soccer players. Our data demonstrated excellent levels of agreement between both dynamometers during the squeeze strength test, suggesting the Smart Groin Trainer as a valid, reliable, and accurate device to measure isometric hip adduction strength.

Load-power relationship in older adults: The influence of maximal mean and peak power values and their associations with lower and upper-limb functional capacity.

Identifying the relative loads (%1RM) that maximize power output (Pmax-load) in resistance exercises can help design interventions to optimize muscle power in older adults. Moreover, examining the maximal mean power (MPmax) and peak power (PPmax) values (Watts) would allow an understanding of their differences and associations with functionality markers in older adults. Therefore, this research aimed to 1) analyze the load-mean and peak power relationships in the leg press and chest press in older adults, 2) examine the differences between mean Pmax-load (MPmax-load) and peak Pmax-load (PPmax-load) within resistance exercises, 3) identify the differences between resistance exercises in MPmax-load and PPmax-load, and 4) explore the associations between MPmax and PPmax in the leg press and chest press with functional capacity indicators. Thirty-two older adults (79.3 ± 7.3 years) performed the following tests: medicine ball throw (MBT), five-repetition sit-to-stand (STS), 10-m walking (10 W), and a progressive loading test in the leg press and chest press. Quadratic regressions analyzed 1) the load-mean and peak power relationships and identified the MPmax-load, MPmax, PPmax-load, and PPmax in both exercises, 2) the associations between MPmax and PPmax in the chest press with MBT, and 3) the associations between MPmax and PPmax in the leg press with STSpower and 10Wvelocity. In the leg press, the MPmax-load was ∼66% 1RM, and the PPmax-load was ∼62% 1RM, both for women and men (p > 0.05). In the chest press, the MPmax-load was ∼62% 1RM, and the PPmax-load was ∼56% 1RM, both for women and men (p > 0.05). There were differences between MPmax-load and PPmax-load within exercises (p < 0.01) and differences between exercises in MPmax-load and PPmax-load (p < 0.01). The MPmax and PPmax in the chest press explained ∼48% and ∼52% of the MBT-1 kg and MBT-3 kg variance, respectively. In the leg press, the MPmax and PPmax explained ∼59% of STSpower variance; however, both variables could not explain the 10Wvelocity performance (r 2 ∼ 0.02). This study shows that the Pmax-load is similar between sexes, is resistance exercise-specific, and varies within exercises depending on the mechanical power variable used in older adults. Furthermore, this research demonstrates the influence of the MBT as an upper-limb power marker in older adults.

Force-velocity relationship in Paralympic powerlifting: two or multiple-point methods to determine a maximum repetition.

BACKGROUND: Due to the absence of evidence in the literature on Paralympic Powerlifting the present study investigated various methods to assess bench press maximum repetition and the way each method influences the measurement of minimum velocity limit (MVT), load at zero velocity (LD0), and force-velocity (FV). OBJECTIVE: To evaluate the precision of the multi-point method using proximal loads (40, 50, 60, 70, 80, and 90% of one repetition maximum; 1RM) compared to the four-point method (50, 60, 70, and 80% of 1RM) and the two-point method using distant loads (40 and 80% and 50 and 80% of 1RM) in in the MVT, LD0, and FV, in bench press performed by Paralympic Powerlifters (PP). METHODS: To accomplish this, 15 male elite PP athletes participated in the study (age: 27.7 ± 5.7 years; BM: 74.0 ± 19.5 kg). All participants performed an adapted bench press test (free weight) with 6 loads (40, 50, 60, 70, 80, and 90% 1RM), 4 loads (50, 60, 70, and 80% 1RM), and 2 loads (40-80% and 50-80% 1RM). The 1RM predictions were made by MVT, LD0, and FV. RESULTS: The main results indicated that the multiple (4 and 6) pointsmethod provides good results in the MVT (R2 = 0.482), the LD0 (R2 = 0.614), and the FV (R2 = 0.508). The two-point method (50-80%) showed a higher mean in MVT [1268.2 ± 502.0 N; ICC95% 0.76 (0.31-0.92)], in LD0 [1504.1 ± 597.3 N; 0.63 (0.17-0.86)], and in FV [1479.2 ± 636.0 N; 0.60 (0.10-0.86)]. CONCLUSION: The multiple-point method (4 and 6 points) and the two-point method (40-80%) using the MVT, LD0, and FV all showed a good ability to predict bench press 1RM in PP.

Maximum Isometric and Dynamic Strength of Mixed Martial Arts Athletes According to Weight Class and Competitive Level.

Mixed martial arts (MMA) athletes must achieve high strength levels to face the physical demands of an MMA fight. This study compared MMA athletes' maximal isometric and dynamic strength according to the competitive level and weight class. Twenty-one male MMA athletes were divided into lightweight professional (LWP; n = 9), lightweight elite (LWE; n = 4), heavyweight professional (HWP; n = 4), and heavyweight elite (HWE; n = 4). The handgrip and isometric lumbar strength tests assessed the isometric strength, and the one-repetition maximum (1RM) bench press and 4RM leg press the dynamic strength. Univariate ANOVA showed differences between groups in absolute and relative 1RM bench press and absolute isometric lumbar strength. Post hoc tests showed differences in 1RM bench press between HWE and LWE (117.0 ± 17.8 kg vs. 81.0 ± 10.0 kg) and HWE and LWP athletes (117.0 ± 17.8 kg vs. 76.7 ± 13.7 kg; 1.5 ± 0.2 kg·BW-1 vs. 1.1 ± 0.2 kg·BW-1). In addition, there was a correlation between 1RM bench press and isometric lumbar strength for absolute (r = 0.67) and relative values (r = 0.50). This study showed that the 1RM bench press and isometric lumbar strength were associated and could differentiate MMA athletes according to their competitive level and weight class. Therefore, optimizing the force production in the upper body and lower back seems important in elite and professional MMA athletes.

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems.

Glioblastoma (GB) is the most malignant form of primary astrocytoma, accounting for more than 60% of all brain tumors in adults. Nowadays, due to the development of multidrug resistance causing relapses to the current treatments and the development of severe side effects resulting in reduced survival rates, new therapeutic approaches are needed. The genus Plectranthus belongs to the Lamiaceae family and is known to be rich in abietane-type diterpenes, which possess antitumor activity. Specifically, P. hadiensis (Forssk.) Schweinf. ex Sprenger has been documented for the use against brain tumors. Therefore, the aim of this work was to perform the bioguided isolation of compounds from the acetonic extract of P. hadiensis stems and to investigate the in vitro antiglioblastoma activity of the extract and its isolated constituents. After extraction, six fractions were obtained from the acetonic extract of P. hadiensis stems. In a preliminary biological screening, the fractions V and III showed the highest antioxidant and antimicrobial activities. None of the fractions were toxic in the Artemia salina assay. We obtained different abietane-type diterpenes such as 7α-acetoxy-6ß-hydroxyroyleanone (Roy) and 6ß,7ß-dihydroxyroyleanone (DiRoy), which was also in agreement with the HPLC-DAD profile of the extract. Furthermore, the antiproliferative activity was assessed in a glioma tumor cell line panel by the Alamar blue assay. After 48 h treatment, Roy exerted strong antiproliferative/cytotoxic effects against tumor cells with low IC50 values among the different cell lines. Finally, we synthesized a new fluorescence derivative in this study to evaluate the biodistribution of Roy. The uptake of BODIPY-7α-acetoxy-6ß-hydroxyroyleanone by GB cells was associated with increased intracellular fluorescence, supporting the antiproliferative effects of Roy. In conclusion, Roy is a promising natural compound that may serve as a lead compound for further derivatization to develop future therapeutic strategies against GB.

Young Swimmers' Classification Based on Performance and Biomechanical Determinants: Determining Similarities Through Cluster Analysis.

The aim of this study was to classify and identify young swimmers' performance, and biomechanical determinant factors, and understand if both sexes can be clustered together. Thirty-eight swimmers of national level (22 boys: 15.92 ± 0.75 years and 16 girls: 14.99 ± 1.06 years) were assessed. Performance (swim speed at front crawl stroke) and a set of kinematic, efficiency, kinetic, and hydrodynamic variables were measured. Variables related to kinetics and efficiency (p < .001) were the ones that better discriminated the clusters. All three clusters included girls. Based on the interaction of these determinant factors, there are girls who can train together with boys. These findings indicate that not understanding the importance of the interplay between such determinants may lead to performance suppression in girls.