Mixed testicular germ cell tumor presenting as metastatic pure choriocarcinoma involving the maxillary gingiva.

Gingival metastases are infrequent and invariably associated with a widespread disease and a poor prognosis. Because of their unremarkable clinical appearance, they can be difficult to distinguish from more common gingival hyperplastic or reactive lesions, such as pyogenic granuloma, peripheral giant cell granuloma, and peripheral ossifying granuloma. We are reporting here an unusual case of a 36-year-old man with a mixed testicular germ cell tumor presenting as a metastatic pure choriocarcinoma involving the maxillary gingiva, extending from the first left premolar to the left second maxillary molar, mimicking a 'benign looking' gingival mass. Gingival metastases may be the first manifestation of a widespread metastatic disease and therefore particular attention must be paid to gingival lesions associated with atypical clinical symptoms and/or signs.

Comparative efficiency of plasma and halogen light sources on composite micro-hardness in different curing conditions.

OBJECTIVES: Recent developments have led to the introduction of high power curing lights, which are claimed to greatly reduce the total curing time. This study evaluated the effectiveness of a plasma-curing device (Apollo 95 E) and a halogen device (Heliolux DLX), in different curing conditions. METHOD: Vicker's micro-hardness values were performed on 1 and 2 mm thick composite discs cured in a natural tooth mold by direct irradiation or indirect irradiation through composite material (2 or 4 mm) and dental tissues (1 mm enamel or 2 mm enamel-dentin). Measures were, respectively, performed after a 1, 3, 6 s (SC, step curing mode) or 18 s (3xSC) exposure to the plasma light, and a 5, 10, 20 or 40 s exposure to the halogen light. RESULTS: With the PAC light used, a 3 s irradiation in the direct curing condition was necessary to reach hardness values similar to those obtained after a 40 s exposure to the halogen light. Using the indirect curing condition, hardness values reached after an 18 s exposure (3xSC mode) with the plasma light were either equivalent or inferior to those obtained with 40 s halogen irradiation. SIGNIFICANCE: Direct polymerization with the plasma light used requires longer exposure times than those initially proposed by the manufacturer. The effectiveness of plasma generated light was lowered by composite or natural tissues, and therefore requires an important increase in the irradiation time when applied to indirect polymerization. The practical advantage of this polymerization method is less than expected, when compared to traditional halogen curing.

Enhancing protein solubilization with nondetergent sulfobetaines.

A significant increase in the yield of protein extraction by the addition of sulfobetaine-type mild solubilization agents is shown for microsomal membrane proteins and proteins from lyophilized platelets, with a maximum increase in yield of up to 100%. These agents are also applied to native isoelectric focusing in immobilized pH gradients. The absence of significant denaturation induced by these solubilizers is shown for creatine kinase.