RESUMO
Plants use RNA silencing as a strong defensive barrier against virus challenges, and viruses counteract this defence by using RNA silencing suppressors (RSSs). With the objective of identifying host factors helping either the plant or the virus in this interaction, we have performed a yeast two-hybrid screen using P1b, the RSS protein of the ipomovirus Cucumber vein yellowing virus (CVYV, family Potyviridae), as a bait. The C-8 sterol isomerase HYDRA1 (HYD1), an enzyme involved in isoprenoid biosynthesis and cell membrane biology, and required for RNA silencing, was isolated in this screen. The interaction between CVYV P1b and HYD1 was confirmed in planta by Bimolecular Fluorescence Complementation assays. We demonstrated that HYD1 negatively impacts the accumulation of CVYV P1b in an agroinfiltration assay. Moreover, expression of HYD1 inhibited the infection of the potyvirus Plum pox virus, especially when antiviral RNA silencing was boosted by high temperature or by coexpression of homologous sequences. Our results reinforce previous evidence highlighting the relevance of particular composition and structure of cellular membranes for RNA silencing and viral infection. We report a new interaction of an RSS protein from the Potyviridae family with a member of the isoprenoid biosynthetic pathway.
Assuntos
Arabidopsis/enzimologia , Proteínas do Capsídeo/metabolismo , Oxirredutases/metabolismo , Vírus Eruptivo da Ameixa/metabolismo , Interferência de RNA , Esteroide Isomerases/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Expressão Gênica , Proteínas de Fluorescência Verde , Mutação , Oxirredutases/genética , Doenças das Plantas/virologia , Folhas de Planta/metabolismo , Folhas de Planta/virologia , Vírus Eruptivo da Ameixa/genética , Vírus Eruptivo da Ameixa/patogenicidade , Ligação Proteica , Esteroide Isomerases/genética , Temperatura , Nicotiana/metabolismo , Nicotiana/virologia , Técnicas do Sistema de Duplo-Híbrido , Regulação para CimaRESUMO
The asymmetric cell divisions necessary for stomatal lineage initiation and progression in Arabidopsis (Arabidopsis thaliana) require the function of the basic helix-loop-helix (bHLH) transcription factor SPEECHLESS (SPCH). Mutants lacking SPCH do not produce stomata or lineages. Here, we isolated a new spch-5 allele carrying a point mutation in the bHLH domain that displayed normal growth, but had an extremely low number of sometimes clustered stomata in the leaves, whereas the hypocotyls did not have any stomata. In vivo tracking of leaf epidermal cell divisions, combined with marker lines and genetic analysis, showed that the spch-5 leaf phenotype is dosage dependent and results from the decreased ability to initiate and amplify lineages, defects in asymmetric cell fate allocation, and misorientation of asymmetric division planes. Notably, application of brassinosteroids (BRs) partly rescued the stomatal leaf phenotype of spch-5 Transcriptomic analysis combining spch-5 with BR treatments revealed that the expression of a set of SPCH target genes was restored by BRs. Our results also show that BR-dependent stomata formation and expression of some, but not all, SPCH target genes require the integrity of the bHLH domain of SPCH.
Assuntos
Proteínas de Arabidopsis/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Brassinosteroides/metabolismo , Mutação , Estômatos de Plantas/fisiologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/metabolismo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Brassinosteroides/farmacologia , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Diferenciação Celular , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica de Plantas , Hipocótilo/citologia , Folhas de Planta/citologia , Folhas de Planta/genética , Estômatos de Plantas/citologia , Estômatos de Plantas/efeitos dos fármacos , Plantas Geneticamente Modificadas , Domínios Proteicos , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Epidermal differentiation in Arabidopsis thaliana aerial organs involves stomatal lineage development. Lineages derive from meristemoids, which arise from asymmetric divisions of protodermal cells. Each meristemoid divides repeatedly in an inward spiral before it transits to a guard mother cell (GMC) that produces the stoma, leaving a trail of surrounding stomatal lineage ground cells (SLGCs) that eventually differentiate into endoreplicated pavement cells. MUTE is a bHLH transcription factor that is expressed in late meristemoids and drives their transition to GMCs. Loss-of-function mute mutants are stomata-less dwarf plants with arrested lineages, in which stunted putative SLGCs surround a halted meristemoid. We analysed MUTE functions using a chemically inducible system for mute-3 complementation based on conditional MUTE expression in its normal domain. Continuous induction from germination produced stomata-bearing, normal-sized plants with viable mute-3 seeds. In 2-week-old mute-3 cotyledons, meristemoids appeared to retain their identity and synchronously formed stomata in response to induced MUTE expression. However, arrested SLGCs were not complemented: many produced stomata, leading to stomatal clusters, and others remained unexpanded and diploid. In contrast, non-lineage pavement cells, which are under-endoreplicated in mute-3, expanded and increased their ploidy level upon induction, showing that the lack of response of SLGCs is specific to this arrested cell type. Leaf phenotypic mosaics include wild-type lineages and adjacent mute-3 lineages, whose meristemoids and putative SLGCs remained arrested, indicating that the role of MUTE in SLGC fate is strictly lineage-autonomous. These results show that timely MUTE expression is essential to prevent stomatal fate in SLGCs and to promote their differentiation as pavement cells.
Assuntos
Proteínas de Arabidopsis/fisiologia , Arabidopsis/crescimento & desenvolvimento , Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia , Diferenciação Celular/genética , Estômatos de Plantas/crescimento & desenvolvimento , Arabidopsis/citologia , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Estradiol/farmacologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Marcadores Genéticos , Fenótipo , Estômatos de Plantas/genética , Estômatos de Plantas/ultraestrutura , PloidiasRESUMO
In angiosperms, shoot branching greatly determines overall plant architecture and affects fundamental aspects of plant life. Branching patterns are determined by genetic pathways conserved widely across angiosperms. In Arabidopsis thaliana (Brassicaceae, Rosidae) BRANCHED1 (BRC1) plays a central role in this process, acting locally to arrest axillary bud growth. In tomato (Solanum lycopersicum, Solanaceae, Asteridae) we have identified two BRC1-like paralogues, SlBRC1a and SlBRC1b. These genes are expressed in arrested axillary buds and both are down-regulated upon bud activation, although SlBRC1a is transcribed at much lower levels than SlBRC1b. Alternative splicing of SlBRC1a renders two transcripts that encode two BRC1-like proteins with different C-t domains due to a 3'-terminal frameshift. The phenotype of loss-of-function lines suggests that SlBRC1b has retained the ancestral role of BRC1 in shoot branch suppression. We have isolated the BRC1a and BRC1b genes of other Solanum species and have studied their evolution rates across the lineages. These studies indicate that, after duplication of an ancestral BRC1-like gene, BRC1b genes continued to evolve under a strong purifying selection that was consistent with the conserved function of SlBRC1b in shoot branching control. In contrast, the coding sequences of Solanum BRC1a genes have evolved at a higher evolution rate. Branch-site tests indicate that this difference does not reflect relaxation but rather positive selective pressure for adaptation.
Assuntos
Regulação da Expressão Gênica de Plantas/fisiologia , Proteínas de Plantas/metabolismo , Brotos de Planta/crescimento & desenvolvimento , Solanum lycopersicum/crescimento & desenvolvimento , Sequência de Aminoácidos , Mapeamento Cromossômico , Evolução Molecular , Duplicação Gênica , Solanum lycopersicum/genética , Solanum lycopersicum/metabolismo , Solanum lycopersicum/ultraestrutura , Dados de Sequência Molecular , Mutação , Fenótipo , Filogenia , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Folhas de Planta/ultraestrutura , Proteínas de Plantas/genética , Brotos de Planta/genética , Brotos de Planta/metabolismo , Brotos de Planta/ultraestrutura , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/metabolismo , Plantas Geneticamente Modificadas/ultraestrutura , Mutação Puntual , RNA Mensageiro/genética , Alinhamento de Sequência , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
TCP genes encode plant-specific transcription factors with a bHLH motif that allows DNA binding and protein-protein interactions. The TCP gene family has five members in the lycophytes and >20 members in the eudicots. Gene duplication and diversification has generated two clades (class I and II) with slightly different TCP domains. Here, we summarize our current knowledge of the evolution of this family, their regulation, the biochemical activity of their proteins and the biological function of some members, in particular, in the control of cell proliferation in developing tissues. Increasing knowledge of the functions of TCP genes should enable their use as tools to modulate plant growth patterns and to generate novel morphologies in species of agronomical interest.
Assuntos
Proteínas de Plantas/genética , Plantas/genética , Fatores de Transcrição/genética , Animais , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Filogenia , Desenvolvimento Vegetal , Proteínas de Plantas/química , Plantas/química , Fatores de Transcrição/químicaRESUMO
We have characterized Arabidopsis esd1 mutations, which cause early flowering independently of photoperiod, moderate increase of hypocotyl length, shortened inflorescence internodes, and altered leaf and flower development. Phenotypic analyses of double mutants with mutations at different loci of the flowering inductive pathways suggest that esd1 abolishes the FLC-mediated late flowering phenotype of plants carrying active alleles of FRI and of mutants of the autonomous pathway. We found that ESD1 is required for the expression of the FLC repressor to levels that inhibit flowering. However, the effect of esd1 in a flc-3 null genetic background and the downregulation of other members of the FLC-like/MAF gene family in esd1 mutants suggest that flowering inhibition mediated by ESD1 occurs through both FLC-and FLC-like gene-dependent pathways. The ESD1 locus was identified through a map-based cloning approach. ESD1 encodes ARP6, a homolog of the actin-related protein family that shares moderate sequence homology with conventional actins. Using chromatin immunoprecipitation (ChIP) experiments, we have determined that ARP6 is required for both histone acetylation and methylation of the FLC chromatin in Arabidopsis.
Assuntos
Actinas/metabolismo , Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Cromatina/metabolismo , Reguladores de Crescimento de Plantas/biossíntese , Acetilação , Actinas/química , Actinas/genética , Alelos , Sequência de Aminoácidos , Proteínas de Arabidopsis/genética , Cromatina/genética , Imunoprecipitação da Cromatina , Sequência Conservada , Regulação para Baixo , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Teste de Complementação Genética , Glucuronidase/análise , Histocitoquímica , Histonas/metabolismo , Metilação , Dados de Sequência Molecular , Mutação , Reguladores de Crescimento de Plantas/genética , Homologia de Sequência de AminoácidosRESUMO
Domestication and genetic improvement of trees is far behind that of herbaceous plants owing to their long generation times, which result from the existence of a long juvenile phase of reproductive incompetence. During recent years, significant progress has been made towards understanding the molecular basis of flowering transition in model herbaceous species. Some of the genes identified have been shown to efficiently accelerate reproductive development when ectopically expressed in transgenic plants, including trees. These results provide new clues as to the molecular basis of reproductive competence in trees and suggest ways to accelerate their genetic improvement.