[Isolation of new serovars of Salmonella in streams of water]. / Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas.

The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001% brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76% of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS)

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas / Isolation of new serum varieties of Salmonella in water streams

Se continuaron las investigaciones tendientes a determinar las causas y grado de contaminación de las aguas del arroyo Zaimán, del distrito Capital de la provincia de Misiones. Se investigó la presencia de serovars del género Salmonella, en muestras extraídas de 2 puntos equidistantes de un establecimiento frigorífico y matadero, en ambos sentidos de la corriente. Se aplicaron técnicas de incubación de alta temperatura, con plaqueos sucesivos efectuados a distintos tiempos de incubación. Como medios de enriquecimiento se emplearon Caldo-Dulcitol Selenito y Caldo-Tetrationato con Verde Brilhante al 0,001%. Para el aislamiento de colonias se utilizaron Agar Eosina-Azul de metileno, Agar Verde Brilhante con Sacarosa y Agar Sulfito de Bismuto. La mejor combinación resultó ser: Caldo Dulcitol Selenito-Agar Sulfito de bismuto. Se caracterizaron las cepas por medio de 10 reacciones bioquímicas y por serología. Se realizaron 34 operaciones de muestreo, hallando Salmonella en un 76% de las muestras analizadas, describiéndose un total de 22 serovariedades. Se señala a S. saphra como la serovar de mayor abundancia, ocupando S. anatum y S. newport el segundo lugar en forma compartida. La mayor parte de los aislamientos y serovars confirmados, provinieron del punto de muestreo ubicado aguas abajo del establecimento industrial, lo que demuestra la influencia de vertido de efluentes en el grado de contaminación de las aguas del arroyo Zaimán, considerándose que el nivel de Salmonella hallado constituye un riesgo potencial significativo para los probladores ribereños que utilizan estas aguas, principalmente con fines de recreación. Se destaca la detección de una nueva seroviedad en el orden mundial, designada como S. zaimán y la presencia de los primeros aislamientos en la Argentina de las serovars S. jos, S. madelia y S. belem, todas pertenecientes a la sub-especie I y la S. IV (HARMELEN) que corresponde a la sub-especie IV del género Salmonella

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas. / [Isolation of new serovars of Salmonella in streams of water]

The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001

brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76

of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS)

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas / Isolation of new serum varieties of Salmonella in water streams

Se continuaron las investigaciones tendientes a determinar las causas y grado de contaminación de las aguas del arroyo Zaimán, del distrito Capital de la provincia de Misiones. Se investigó la presencia de serovars del género Salmonella, en muestras extraídas de 2 puntos equidistantes de un establecimiento frigorífico y matadero, en ambos sentidos de la corriente. Se aplicaron técnicas de incubación de alta temperatura, con plaqueos sucesivos efectuados a distintos tiempos de incubación. Como medios de enriquecimiento se emplearon Caldo-Dulcitol Selenito y Caldo-Tetrationato con Verde Brilhante al 0,001%. Para el aislamiento de colonias se utilizaron Agar Eosina-Azul de metileno, Agar Verde Brilhante con Sacarosa y Agar Sulfito de Bismuto. La mejor combinación resultó ser: Caldo Dulcitol Selenito-Agar Sulfito de bismuto. Se caracterizaron las cepas por medio de 10 reacciones bioquímicas y por serología. Se realizaron 34 operaciones de muestreo, hallando Salmonella en un 76% de las muestras analizadas, describiéndose un total de 22 serovariedades. Se señala a S. saphra como la serovar de mayor abundancia, ocupando S. anatum y S. newport el segundo lugar en forma compartida. La mayor parte de los aislamientos y serovars confirmados, provinieron del punto de muestreo ubicado aguas abajo del establecimento industrial, lo que demuestra la influencia de vertido de efluentes en el grado de contaminación de las aguas del arroyo Zaimán, considerándose que el nivel de Salmonella hallado constituye un riesgo potencial significativo para los probladores ribereños que utilizan estas aguas, principalmente con fines de recreación. Se destaca la detección de una nueva seroviedad en el orden mundial, designada como S. zaimán y la presencia de los primeros aislamientos en la Argentina de las serovars S. jos, S. madelia y S. belem, todas pertenecientes a la sub-especie I y la S. IV (HARMELEN) que corresponde a la sub-especie IV del género Salmonella (AU)

[Salmonella: its incidence in waters of the Zaimán arroyo]. / Salmonella: su incidencia en aguas del arroyo Zaimán.

The present work is the initial stage of a project to determine the causes and degree of pollution of the Zaiman creek waters, which flow through wide suburban areas of Posadas, Argentina. The research was focused on the presence of Salmonella serotypes, pathogenic microorganisms probably responsible for most waterborne disease outbreaks. In addition, the N.M.P./100 ml (Most Probable Number) of total coliform organisms was determined in samples collected from points near cold storage plant and slaughterhouse, both to show the pollution effect of industrial discharges. So far, 35 sampling operations have been carried out in representative geographic points. The methodology for Salmonella consisted in applying concentration technics for waterborne pathogens, followed by an enrichment procedure performed by incubating samples in Dulcitol Selenite and Tetrathionate (Muller-Kauffman) broths at 41.5 degrees C during 25 hours. Typical, well-isolated Salmonella-like colonies were obtained by streaking duplicate plates of E.M.B. Agar (Eosin Methylene Blue), Brilliant Green Agar and Bismuth Sulphite Agar. Suspected colonies were classified by 10 biochemical reactions and further verified by serological identification, performed at the National Institute of Microbiology "Carlos Malbrán". The methodology followed for the determination of total coliforms was the one described in the American Water Standards. Salmonella serotypes were isolated in 46% of the examined samples. Isolation efficiency of culture media is shown. First isolations of Salmonella abaetetuba and S. saphra are reported. Most serotypes were isolated from sampling points down-stream from the industrial plant. Zaimán creek waters were classified as No 4 of W.H.O (World Health Organization) stream waters classification, except during heavy rain periods in which they ranked as No 3.

Salmonella: su incidencia en aguas del arroyo Zaimán. / [Salmonella: its incidence in waters of the Zaimán arroyo]

The present work is the initial stage of a project to determine the causes and degree of pollution of the Zaiman creek waters, which flow through wide suburban areas of Posadas, Argentina. The research was focused on the presence of Salmonella serotypes, pathogenic microorganisms probably responsible for most waterborne disease outbreaks. In addition, the N.M.P./100 ml (Most Probable Number) of total coliform organisms was determined in samples collected from points near cold storage plant and slaughterhouse, both to show the pollution effect of industrial discharges. So far, 35 sampling operations have been carried out in representative geographic points. The methodology for Salmonella consisted in applying concentration technics for waterborne pathogens, followed by an enrichment procedure performed by incubating samples in Dulcitol Selenite and Tetrathionate (Muller-Kauffman) broths at 41.5 degrees C during 25 hours. Typical, well-isolated Salmonella-like colonies were obtained by streaking duplicate plates of E.M.B. Agar (Eosin Methylene Blue), Brilliant Green Agar and Bismuth Sulphite Agar. Suspected colonies were classified by 10 biochemical reactions and further verified by serological identification, performed at the National Institute of Microbiology [quot ]Carlos Malbrán[quot ]. The methodology followed for the determination of total coliforms was the one described in the American Water Standards. Salmonella serotypes were isolated in 46

of the examined samples. Isolation efficiency of culture media is shown. First isolations of Salmonella abaetetuba and S. saphra are reported. Most serotypes were isolated from sampling points down-stream from the industrial plant. Zaimán creek waters were classified as No 4 of W.H.O (World Health Organization) stream waters classification, except during heavy rain periods in which they ranked as No 3.