El extracto acuoso de Phyllanthus orbicularis K protege al ADN plasmídico del daño inducido por las radiaciones ultravioletas / Phyllanthus orbicularis K aqueous extract protect plasmid DNA against the damage induced by ultraviolet radiation

Objetivo: Evaluar el efecto protector del extracto acuoso de Phyllanthus orbicularis, K ante el daño inducido por las radiaciones UVB y UVC. Material y métodos: Se empleó el ensayo con ADN plasmídico (pBluescript SK II) libre de célula. Se evaluó la capacidad del extracto acuoso de P. orbicularis de inducir roturas de cadenas en el plásmido, a las concentraciones 0,0001-2,0 mg/ml. Se realizaron estudios de protección del extracto frente a las radiaciones UVB y UVC a las concentraciones de 0,1-2,0 mg/ml. Se cuantificó la transmitancia del extracto frente ambos tipos de radiaciones. Resultados: Ninguna de las concentraciones evaluadas resultó genotóxica en 30 min de exposición. Las concentraciones ≥ 1 mg/ml de P. Obicularis sí indujeron roturas de cadenas a tiempos mayores de evaluación. El extracto de P. Orbicularis protegió al ADN frente a las radiaciones UVB y UVC a concentraciones ≥ 0,1 mg/ml y 0,5 mg/ml respectivamente. Conclusiones: En nuestras condiciones experimentales, el extracto acuoso de P. Orbicularis proteg eal ADN frente al daño inducido por las radiaciones UV (AU)

Aim: The aim of this work was to evaluate the protective effect of aqueous extract of Phyllanthus Orbicularis, K from the damage induced by UV radiation. Material and methods: The plasmid-based non cellular system was used. The extract capacity to induce DNA strand breaks was evaluated at 0.0001- 2.0 mg/ml concentrations. The protective effect of extract against UVB and UVC radiation was evaluated at 0.1- 2.0 mg/ml concentrations. The transmittance of extract was measured for both UV radiations. Results: The P. orbicularis aqueous extract was not genotoxic even after 30 min of exposure. Concentrations ≥ 1 mg/ml of extract induced strand breaks at major times of exposition. P. Orbicularis extract protected DNA against UVB and UVC radiation at concentrations ≥ 0.1 mg/ml and 0.5 mg/ml respectively. Conclusions: In our experimental conditions, P. Orbicularis aqueous extract protects DNA from damage induced by UV radiation (AU)

Chemotherapy and the future: microdialysis as a local administration technique.

Chemotherapy has an important role in cancer treatment. Although there have been developments and good results, chemotherapy still has many limitations mainly due to its toxicity and to the resistance mechanisms of tumour cells. Therefore, besides other important improvements in chemotherapy agents and indications, recent researches have focused on the development of locoregional administration techniques, with which therapeutic weapons can reach the tumour with a higher concentration and fewer side-effects. At present, local chemotherapy includes delivery systems or prodrug strategies, arterial infusions, intraperitoneal administration and aerosolised agents. We will describe a new local cancer chemotherapy method, using microdyalisis procedures, which may revolutionise the actual tumour management because of the higher effectiveness and the absence of side-effects. Finally, the applications and limitations of this technique will be considered.