Proinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcoma.

BACKGROUND: Photodynamic therapy (PDT) is an anticancer therapy that associates the photosensitizer (PS), oxygen and light to destroy cancer cells. Methylene blue (MB) is considered a second generation phenothiazine dye with excellent photochemical properties. AIM: To evaluate whether MB-mediated PDT can induce oxidative stress and inflammation, therefore, interfering tumor growth. MATERIALS AND METHODS: The study was conducted on Wistar rats transplanted with Walker 256 carcinosarcoma (W256). The proinflammatory interleukins levels (IL-1ß, IL-6, IL-10, TNF-α) were determined by ELISA, mRNA expression of COX-1, COX-2, iNOS and eNOS by RT-PCR, lipid peroxidation was measured by the TBARS method. Moreover, myeloperoxidase (MPO) activity in neutrophils was determined by MPO activity assay. All indices mentioned above were determined in tumor tissue. Kaplan - Meier and Gehan - Breslow - Wilcoxon tests were used for survival analysis. RESULTS: We found that the treatment of W256 with 0.1% MB + 1 J/cm2 provoked a significant increase in the interleukins levels (IL-1ß, IL-6, IL-10, TNF-α), prostaglandin E2, the mRNA expression of COX-2, iNOS, lipid peroxidation and MPO activity in tumor tissue, which were statistically different (p < 0.05) compared to other experimental and control groups. The results of the estimation of survival curves show a greater probability of survival in 0.1% MB + 1 J/cm2 (total energy dose =142.8 J/cm2) treated group. CONCLUSION: Our results suggest that treatment of W256 with 0.1% MB + 1 J/cm2 was able to promote cytotoxic effects in tumor tissue by the generation of reactive oxygen species causing inflammation and thus interfering in the tumor growth.

Proinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcoma

Photodynamic therapy (PDT) is an anticancer therapy that associates the photosensitizer (PS), oxygen and light to destroy cancer cells. Methylene blue (MB) is considered a second generation phenothiazine dye with excellent photochemical properties. Aim: To evaluate whether MB-mediated PDT can induce oxidative stress and inflammation, therefore, interfering tumor growth. Materials and Methods: The study was conducted on Wistar rats transplanted with Walker 256 carcinosarcoma (W256). The proinflammatory interleukins levels (IL-1ß, IL-6, IL-10, TNF-a) were determined by ELISA, mRNA expression of COX-1, COX-2, iNOS and eNOS by RT-PCR, lipid peroxidation was measured by the TBARS method. Moreover, myeloperoxidase (MPO) activity in neutrophils was determined by MPO activity assay. All indices mentioned above were determined in tumor tissue. Kaplan — Meier and Gehan — Breslow — Wilcoxon tests were used for survival analysis. Results: We found that the treatment of W256 with 0.1% MB + 1 J/cm2 provoked a significant increase in the interleukins levels (IL-1ß, IL-6, IL-10, TNF-a), prostaglandin E2, the mRNA expression of COX-2, iNOS, lipid peroxidation and MPO activity in tumor tissue, which were statistically different (p < 0.05) compared to other experimental and control groups. The results of the estimation of survival curves show a greater probability of survival in 0.1% MB + 1 J/cm2 (total energy dose =142.8 J/cm2 ) treated group. Conclusion: Our results suggest that treatment of W256 with 0.1% MB + 1 J/cm2 was able to promote cytotoxic effects in tumor tissue by the generation of reactive oxygen species causing inflammation and thus interfering in the tumor growth

A Promising Antiprion Trimethoxychalcone Binds to the Globular Domain of the Cellular Prion Protein and Changes Its Cellular Location.

The search for antiprion compounds has been encouraged by the fact that transmissible spongiform encephalopathies (TSEs) share molecular mechanisms with more prevalent neurodegenerative pathologies, such as Parkinson's and Alzheimer's diseases. Cellular prion protein (PrPC) conversion into protease-resistant forms (protease-resistant PrP [PrPRes] or the scrapie form of PrP [PrPSc]) is a critical step in the development of TSEs and is thus one of the main targets in the screening for antiprion compounds. In this work, three trimethoxychalcones (compounds J1, J8, and J20) and one oxadiazole (compound Y17), previously identified in vitro to be potential antiprion compounds, were evaluated through different approaches in order to gain inferences about their mechanisms of action. None of them changed PrPC mRNA levels in N2a cells, as shown by reverse transcription-quantitative real-time PCR. Among them, J8 and Y17 were effective in real-time quaking-induced conversion reactions using rodent recombinant PrP (rPrP) from residues 23 to 231 (rPrP23-231) as the substrate and PrPSc seeds from hamster and human brain. However, when rPrP from residues 90 to 231 (rPrP90-231), which lacks the N-terminal domain, was used as the substrate, only J8 remained effective, indicating that this region is important for Y17 activity, while J8 seems to interact with the PrPC globular domain. J8 also reduced the fibrillation of mouse rPrP23-231 seeded with in vitro-produced fibrils. Furthermore, most of the compounds decreased the amount of PrPC on the N2a cell surface by trapping this protein in the endoplasmic reticulum. On the basis of these results, we hypothesize that J8, a nontoxic compound previously shown to be a promising antiprion agent, may act by different mechanisms, since its efficacy is attributable not only to PrP conversion inhibition but also to a reduction of the PrPC content on the cell surface.

Desempenho de frangos de corte alimentados com inclusão de creatina animal na ração / Performance of broilers fed with inclusion animal creatine in the feed

The objective of this study was to evaluate the productive performance and carcass characteristics of broilers fed with creatine inclusion in the diet. Were evaluated one hundred and sixteen Cobb 500-day-old chicks were divided into four phases: 1 to 7, 8 to 21, 9 to 35 and 22 to 49 days of age, and four experimental diets were formulated, pre-initial, initial, growth and termination, respectively. All diets were produced based on corn and soybean meal. The birds were distributed in a completely randomized design with 0,10% of creatine in substitution to soybean meal and seven replicates. The variables studied were: feed intake (g/bird/day), weight gain (g/bird/day), final weight (g/bird), feed conversion (g/g), viability (%), noble cuts (g), edible offal (g) and carcass yield (%). There was no influence of the inclusion of creatine on the performance parameters of 7 - 21 and 35 days of age. However, there was improvement (P<0,05) in the feed conversion of 7 42 days of age. Body weight, carcass weight, thighs, wings, heart, gizzard, liver and carcass yield were not influenced by the inclusion of creatine, except for weight and breast yield for slaughter at 42 days (P<0,05). The inclusion of 0,10% of creatine in diets for broilers provided higher breast weight of birds slaughtered at 42 days and better feed conversion from 7 to 42 days of age.(AU)

Objetivou-se avaliar o desempenho produtivo e as características de carcaça de frangos de corte alimentados com inclusão de creatina na ração. Foram avaliados 196 pintainhos de um dia de idade da linhagem Cobb 500, divididos em quatro fases: 1 a 7, de 8 a 21, 22 a 33 e 34 a 49 dias de idade, e formuladas quatro dietas experimentais, pré-inicial, inicial, crescimento e terminação, respectivamente. Todas as dietas foram produzidas a base de milho e farelo de soja. As aves foram distribuídas em delineamento inteiramente casualizado, com 0,10% de creatina em substituição ao farelo de soja e sete repetições. As variáveis estudas foram: consumo de ração (g/ave/dia), ganho de peso (g/ave/dia), peso final (g/ave), conversão alimentar (g/g), viabilidade (%), cortes nobres (g), vísceras comestíveis (g) e rendimento de carcaça (%). Não houve influência da inclusão de creatina sobre os parâmetros de desempenho de 7 21 e 35 dias de idade. No entanto, houve melhora (P<0,05) na conversão alimentar de 7 42 dias de idade. Peso vivo, peso da carcaça, coxas, asas, coração, moela, fígado e rendimento de carcaças não foram influenciados pela inclusão de creatina, com exceção do peso e rendimento de peito para abate aos 42 dias (P<0,05). A inclusão de 0,10% de creatina em dietas para frangos de corte proporciona maior peso de peito de aves abatidas aos 42 dias e melhor conversão alimentar de 7 a 42 dias de idade.(AU)

Desempenho e qualidade de ovos de poedeiras alimentadas com níveis de bagaço de cevada na ração / Performance and egg quality of laying hens fed a diet with different levels of barley bagasse

The objective of this work was to evaluate the productive performance and egg quality of semi-heavy laying hens fed a diet with different levels of barley bagasse. Ninety-six laying hens of the Hisex Brown lineage were used after forced molting. The birds were distributed in a completely randomized experimental design with four treatments, six replicates, and four birds per experimental unit. The treatments consisted of four levels of barley bagasse (0, 4, 8 and 12%) as corn substitute. The following variables were evaluated: feed intake (g/bird/day), egg production (%), egg weight (g), egg mass (g/bird/day), feed conversion ratio per egg mass (kg/kg) and per dozen eggs (kg/dz), specific gravity (g/cm3), weight and percentage of yolk (g and %), albumen (g and %) and shell (g and %), variation in body weight (g), and viability of birds (%). There was no effect (P>0.05) of treatments on the quality of the eggs. A quadratic effect (P<0.05) was observed for feed intake, with an estimated level of barley bagasse of 8.36% resulting in the lowest intake. There was a decreasing linear effect (P<0.01) for egg production and egg mass, and an increasing linear effect (P<0.05) for feed conversion per dozen eggs (kg/dz). The use of barley bagasse in the diet does not influence egg quality, but decreases the zootechnical performance of semi-heavy laying hens reared in a hot environment during the second laying cycle.(AU)

Objetivou-se com este trabalho avaliar o desempenho produtivo e a qualidade de ovos de poedeiras semipesadas alimentadas com diferentes níveis de bagaço de cevada na ração. Foram utilizadas 96 poedeiras da linhagem Hisex Brown após a muda forçada. As aves foram distribuídas em delineamento experimental inteiramente casualizado, com quatro tratamentos, seis repetições e quatro aves por unidade experimental. Os tratamentos foram constituídos por quatro níveis de bagaço de cevada (0; 4; 8 e 12%) em substituição ao milho. Foram avaliados: consumo de ração (g/ave/dia), produção de ovos (%), peso dos ovos (g), massa de ovos (g/ave/dia), conversão alimentar por massa (kg/kg) e por dúzia (kg/dz) de ovos, gravidade específica (g/cm3), peso e porcentagem de gema (g e %), albúmen (g e %) e casca (g e %), variação do peso corporal (g) e viabilidade das aves (%). Não houve efeito (P>0,05) dos tratamentos sobre a qualidade dos ovos. Houve efeito quadrático (P<0,05) para o consumo de ração, sendo estimado o nível de 8,36% de bagaço de cevada para o menor consumo. Verificou-se efeito linear (P<0,01) decrescente para a produção de ovos e massa de ovos, e efeito linear crescente (P<0,05) para a conversão alimentar por dúzia de ovos (kg/dz). O uso de bagaço de cevada na ração, não influencia a qualidade dos ovos, contudo diminui o desempenho zootécnico de poedeiras semipesadas criadas em clima quente, no segundo ciclo de postura.(AU)

Desempenho e qualidade de ovos de poedeiras alimentadas com níveis de bagaço de cevada na ração / Performance and egg quality of laying hens fed a diet with different levels of barley bagasse

The objective of this work was to evaluate the productive performance and egg quality of semi-heavy laying hens fed a diet with different levels of barley bagasse. Ninety-six laying hens of the Hisex Brown lineage were used after forced molting. The birds were distributed in a completely randomized experimental design with four treatments, six replicates, and four birds per experimental unit. The treatments consisted of four levels of barley bagasse (0, 4, 8 and 12%) as corn substitute. The following variables were evaluated: feed intake (g/bird/day), egg production (%), egg weight (g), egg mass (g/bird/day), feed conversion ratio per egg mass (kg/kg) and per dozen eggs (kg/dz), specific gravity (g/cm3), weight and percentage of yolk (g and %), albumen (g and %) and shell (g and %), variation in body weight (g), and viability of birds (%). There was no effect (P>0.05) of treatments on the quality of the eggs. A quadratic effect (P<0.05) was observed for feed intake, with an estimated level of barley bagasse of 8.36% resulting in the lowest intake. There was a decreasing linear effect (P<0.01) for egg production and egg mass, and an increasing linear effect (P<0.05) for feed conversion per dozen eggs (kg/dz). The use of barley bagasse in the diet does not influence egg quality, but decreases the zootechnical performance of semi-heavy laying hens reared in a hot environment during the second laying cycle.

Objetivou-se com este trabalho avaliar o desempenho produtivo e a qualidade de ovos de poedeiras semipesadas alimentadas com diferentes níveis de bagaço de cevada na ração. Foram utilizadas 96 poedeiras da linhagem Hisex Brown após a muda forçada. As aves foram distribuídas em delineamento experimental inteiramente casualizado, com quatro tratamentos, seis repetições e quatro aves por unidade experimental. Os tratamentos foram constituídos por quatro níveis de bagaço de cevada (0; 4; 8 e 12%) em substituição ao milho. Foram avaliados: consumo de ração (g/ave/dia), produção de ovos (%), peso dos ovos (g), massa de ovos (g/ave/dia), conversão alimentar por massa (kg/kg) e por dúzia (kg/dz) de ovos, gravidade específica (g/cm3), peso e porcentagem de gema (g e %), albúmen (g e %) e casca (g e %), variação do peso corporal (g) e viabilidade das aves (%). Não houve efeito (P>0,05) dos tratamentos sobre a qualidade dos ovos. Houve efeito quadrático (P<0,05) para o consumo de ração, sendo estimado o nível de 8,36% de bagaço de cevada para o menor consumo. Verificou-se efeito linear (P<0,01) decrescente para a produção de ovos e massa de ovos, e efeito linear crescente (P<0,05) para a conversão alimentar por dúzia de ovos (kg/dz). O uso de bagaço de cevada na ração, não influencia a qualidade dos ovos, contudo diminui o desempenho zootécnico de poedeiras semipesadas criadas em clima quente, no segundo ciclo de postura.

Desempenho de frangos de corte alimentados com inclusão de creatina animal na ração / Performance of broilers fed with inclusion animal creatine in the feed

The objective of this study was to evaluate the productive performance and carcass characteristics of broilers fed with creatine inclusion in the diet. Were evaluated one hundred and sixteen Cobb 500-day-old chicks were divided into four phases: 1 to 7, 8 to 21, 9 to 35 and 22 to 49 days of age, and four experimental diets were formulated, pre-initial, initial, growth and termination, respectively. All diets were produced based on corn and soybean meal. The birds were distributed in a completely randomized design with 0,10% of creatine in substitution to soybean meal and seven replicates. The variables studied were: feed intake (g/bird/day), weight gain (g/bird/day), final weight (g/bird), feed conversion (g/g), viability (%), noble cuts (g), edible offal (g) and carcass yield (%). There was no influence of the inclusion of creatine on the performance parameters of 7 - 21 and 35 days of age. However, there was improvement (P<0,05) in the feed conversion of 7 – 42 days of age. Body weight, carcass weight, thighs, wings, heart, gizzard, liver and carcass yield were not influenced by the inclusion of creatine, except for weight and breast yield for slaughter at 42 days (P<0,05). The inclusion of 0,10% of creatine in diets for broilers provided higher breast weight of birds slaughtered at 42 days and better feed conversion from 7 to 42 days of age.

Objetivou-se avaliar o desempenho produtivo e as características de carcaça de frangos de corte alimentados com inclusão de creatina na ração. Foram avaliados 196 pintainhos de um dia de idade da linhagem Cobb 500, divididos em quatro fases: 1 a 7, de 8 a 21, 22 a 33 e 34 a 49 dias de idade, e formuladas quatro dietas experimentais, pré-inicial, inicial, crescimento e terminação, respectivamente. Todas as dietas foram produzidas a base de milho e farelo de soja. As aves foram distribuídas em delineamento inteiramente casualizado, com 0,10% de creatina em substituição ao farelo de soja e sete repetições. As variáveis estudas foram: consumo de ração (g/ave/dia), ganho de peso (g/ave/dia), peso final (g/ave), conversão alimentar (g/g), viabilidade (%), cortes nobres (g), vísceras comestíveis (g) e rendimento de carcaça (%). Não houve influência da inclusão de creatina sobre os parâmetros de desempenho de 7 – 21 e 35 dias de idade. No entanto, houve melhora (P<0,05) na conversão alimentar de 7 – 42 dias de idade. Peso vivo, peso da carcaça, coxas, asas, coração, moela, fígado e rendimento de carcaças não foram influenciados pela inclusão de creatina, com exceção do peso e rendimento de peito para abate aos 42 dias (P<0,05). A inclusão de 0,10% de creatina em dietas para frangos de corte proporciona maior peso de peito de aves abatidas aos 42 dias e melhor conversão alimentar de 7 a 42 dias de idade.

Sodium levels in the diets of semi-heavy laying hens reared in a hot climate after peak lay / Níveis de sódio na ração de poedeiras semipesadas após o pico de postura criadas em clima quente

The objective of this study was to estimate the sodium (Na) requirements of semi-heavy laying hens reared in a hot climate after peak lay. A total of 120 Hisex Brown hens, 48 weeks of age, were used. The birds were allocated in a completely randomized design consisting of five treatments, six replicates and four animals per experimental unit. The experimental diets were formulated with corn and soybean meal and the treatments consisted of five levels of sodium (0.12, 0.17, 0.22, 0.27 and 0.32%) derived from common salt. The following parameters were evaluated: feed intake (g), egg production (%), egg weight (g), egg mass (g), feed conversion (kg) per kilogram of eggs and per dozen eggs, specific gravity (g/cm3), shell thickness (mm), egg components including egg yolk (g and %), albumin (g and %) and shell (g and %), viability (%), and variation in body weight (g) of the birds. There was no effect (P>0.05) of sodium levels on egg yolk weight (g) or albumin percentage. A decreasing linear effect (P<0.01) was observed for feed intake, which decreased with increasing sodium level in the diet. A quadratic effect (P<0.05) was found for egg production, egg weight, feed conversion per kilogram of eggs and dozen eggs, albumin and shell weight, specific gravity, shell thickness, and percentage of shell and egg yolk. A sodium level higher than 0.27% negatively influenced egg components and zootechnical performance of the birds. The inclusion of 0.20% sodium in the diet after peak lay is recommended for semi-heavy laying hens reared in a hot climate to increase egg quality and productive performance. (AU)

O Objetivou-se com este trabalho estimar a exigência nutricional de sódio (Na) para poedeiras semipesadas criadas em clima quente, após o pico de postura. Foram utilizadas 120 galinhas da linhagem Hisex Brown, com 48 semanas de idade. As aves foram distribuídas em delineamento experimental inteiramente casualizado, com cinco tratamentos, seis repetições e quatro aves por unidade experimental. As rações experimentais foram formuladas a base de milho e farelo de soja e os tratamentos foram constituídos por cinco níveis de sódio (0,12; 0,17; 0,22; 0,27 e 0,32%) derivados do sal comum. Foram avaliados: consumo de ração (g), produção de ovos (%), peso dos ovos (g), massa de ovos (g), conversão alimentar por massa (kg/kg) e por dúzia (kg/dz) de ovos, gravidade específica (g/cm3), espessura de casca (mm), elementos dos ovos, tais como, gema (g e %), albúmen (g e %) e casca (g e %), viabilidade (%) e variação do peso corporal (g) das aves. Não foi verificado efeito (P>0,05) dos níveis de sódio sobre o peso da gema (g) e porcentagem de albúmen. Houve efeito linear (P<0,01) decrescente para o consumo de ração, diminuindo o consumo à medida que aumentou o nível de sódio na ração. Verificou-se efeito quadrático (P<0,01) para a produção de ovos, peso dos ovos, conversão alimentar por massa e dúzia de ovos, pesos de albúmen e casca, gravidade específica, espessura de casca e porcentagem de casca e gema (P<0,05). Os níveis de sódio analisados influenciaram os componentes dos ovos e o desempenho zootécnico das aves acima de 0,27%, negativamente. Recomenda-se a inclusão de 0,20% de sódio na ração de poedeiras semipesadas criadas em clima quente, após o pico de postura, para proporcionar melhor qualidade dos ovos e desempenho produtivo.(AU)

Óleo de soja e sebo bovino na ração de poedeiras semipesadas criadas em regiões de clima quente / Soybean oil and beef tallow in the diet of semi-heavy laying hens reared in hot climate regions

The objective of this study was to evaluate the productive performance and physical quality of eggs from semi-heavy laying hens reared in hot climate regions and fed diets containing soybean oil or beef tallow. The experiment had a duration of 63 days divided into three cycles of 21 days each. A total of 160 semi-heavy Hisex Brown laying hens at 50 weeks of age and with an initial weight of 1.755 ± 0.172 kg were used. The birds were reared in a floor system and housed in boxes. A completely randomized design consisting of two treatments and five replicates was used, with 16 birds per experimental unit (box). Two experimental diets based on corn and soybean meal were formulated and soybean oil or beef tallow was added, corresponding to treatments 1 and 2, respectively. The following parameters were evaluated: egg production (%), feed intake (g/bird/day), egg mass (g/bird/day), feed conversion per egg mass (kg/kg), feed conversion per dozen eggs (kg/dozen), egg weight, percentage of yolk, egg white and shell (%), specific gravity (g/cm3), animal viability (%), and body weight variation (g). No differences (P>0.05) were observed in any of the parameters studied. The dietary inclusion of soybean oil or beef tallow does not influence the productive performance or egg quality of semi-heavy laying hens reared in hot climate regions.(AU)

O objetivo do estudo foi avaliar o desempenho produtivo e qualidade física dos ovos de poedeiras semipesadas criadas em regiões de clima quente, alimentadas com rações contendo óleo de soja ou sebo bovino. O período experimental teve duração de 63 dias, sendo dividido em três ciclos de 21 dias cada. Foram utilizadas 160 poedeiras semipesadas da linhagem Hisex Brown, com 50 semanas de idade e peso inicial de 1,755 ± 0,172 kg. As aves foram criadas em sistema de piso, sendo alojadas em boxes. O delineamento utilizado foi o inteiramente casualizado, composto por dois tratamentos e cinco repetições, com 16 aves por unidade experimental (boxe). Foram formuladas duas dietas experimentais a base de milho e farelo de soja, com a inclusão de óleo de soja e sebo bovino constituindo os tratamentos um e dois respectivamente. Os parâmetros avaliados foram: produção de ovos (%), consumo de ração (g/ave/dia), massa de ovos (g/ave/dia), conversão alimentar por massa de ovos (kg/kg), conversão alimentar por dúzia de ovos (kg/dúzia), peso dos ovos, porcentagem da gema, albúmen e casca (%), gravidade específica (g/cm3), viabilidade das aves (%) e variação do peso corporal (g). Não foram observadas diferenças (P>0,05) em nenhum dos parâmetros avaliados. O uso de óleo de soja ou sebo bovino na ração, não influencia o desempenho produtivo e qualidade dos ovos de poedeiras semipesadas, criadas em regiões de clima quente. (AU)

Óleo de soja e sebo bovino na ração de poedeiras semipesadas criadas em regiões de clima quente / Soybean oil and beef tallow in the diet of semi-heavy laying hens reared in hot climate regions

The objective of this study was to evaluate the productive performance and physical quality of eggs from semi-heavy laying hens reared in hot climate regions and fed diets containing soybean oil or beef tallow. The experiment had a duration of 63 days divided into three cycles of 21 days each. A total of 160 semi-heavy Hisex Brown laying hens at 50 weeks of age and with an initial weight of 1.755 ± 0.172 kg were used. The birds were reared in a floor system and housed in boxes. A completely randomized design consisting of two treatments and five replicates was used, with 16 birds per experimental unit (box). Two experimental diets based on corn and soybean meal were formulated and soybean oil or beef tallow was added, corresponding to treatments 1 and 2, respectively. The following parameters were evaluated: egg production (%), feed intake (g/bird/day), egg mass (g/bird/day), feed conversion per egg mass (kg/kg), feed conversion per dozen eggs (kg/dozen), egg weight, percentage of yolk, egg white and shell (%), specific gravity (g/cm3), animal viability (%), and body weight variation (g). No differences (P>0.05) were observed in any of the parameters studied. The dietary inclusion of soybean oil or beef tallow does not influence the productive performance or egg quality of semi-heavy laying hens reared in hot climate regions.

O objetivo do estudo foi avaliar o desempenho produtivo e qualidade física dos ovos de poedeiras semipesadas criadas em regiões de clima quente, alimentadas com rações contendo óleo de soja ou sebo bovino. O período experimental teve duração de 63 dias, sendo dividido em três ciclos de 21 dias cada. Foram utilizadas 160 poedeiras semipesadas da linhagem Hisex Brown, com 50 semanas de idade e peso inicial de 1,755 ± 0,172 kg. As aves foram criadas em sistema de piso, sendo alojadas em boxes. O delineamento utilizado foi o inteiramente casualizado, composto por dois tratamentos e cinco repetições, com 16 aves por unidade experimental (boxe). Foram formuladas duas dietas experimentais a base de milho e farelo de soja, com a inclusão de óleo de soja e sebo bovino constituindo os tratamentos um e dois respectivamente. Os parâmetros avaliados foram: produção de ovos (%), consumo de ração (g/ave/dia), massa de ovos (g/ave/dia), conversão alimentar por massa de ovos (kg/kg), conversão alimentar por dúzia de ovos (kg/dúzia), peso dos ovos, porcentagem da gema, albúmen e casca (%), gravidade específica (g/cm3), viabilidade das aves (%) e variação do peso corporal (g). Não foram observadas diferenças (P>0,05) em nenhum dos parâmetros avaliados. O uso de óleo de soja ou sebo bovino na ração, não influencia o desempenho produtivo e qualidade dos ovos de poedeiras semipesadas, criadas em regiões de clima quente.

Sodium levels in the diets of semi-heavy laying hens reared in a hot climate after peak lay / Níveis de sódio na ração de poedeiras semipesadas após o pico de postura criadas em clima quente

The objective of this study was to estimate the sodium (Na) requirements of semi-heavy laying hens reared in a hot climate after peak lay. A total of 120 Hisex Brown hens, 48 weeks of age, were used. The birds were allocated in a completely randomized design consisting of five treatments, six replicates and four animals per experimental unit. The experimental diets were formulated with corn and soybean meal and the treatments consisted of five levels of sodium (0.12, 0.17, 0.22, 0.27 and 0.32%) derived from common salt. The following parameters were evaluated: feed intake (g), egg production (%), egg weight (g), egg mass (g), feed conversion (kg) per kilogram of eggs and per dozen eggs, specific gravity (g/cm3), shell thickness (mm), egg components including egg yolk (g and %), albumin (g and %) and shell (g and %), viability (%), and variation in body weight (g) of the birds. There was no effect (P>0.05) of sodium levels on egg yolk weight (g) or albumin percentage. A decreasing linear effect (P<0.01) was observed for feed intake, which decreased with increasing sodium level in the diet. A quadratic effect (P<0.05) was found for egg production, egg weight, feed conversion per kilogram of eggs and dozen eggs, albumin and shell weight, specific gravity, shell thickness, and percentage of shell and egg yolk. A sodium level higher than 0.27% negatively influenced egg components and zootechnical performance of the birds. The inclusion of 0.20% sodium in the diet after peak lay is recommended for semi-heavy laying hens reared in a hot climate to increase egg quality and productive performance.

O Objetivou-se com este trabalho estimar a exigência nutricional de sódio (Na) para poedeiras semipesadas criadas em clima quente, após o pico de postura. Foram utilizadas 120 galinhas da linhagem Hisex Brown, com 48 semanas de idade. As aves foram distribuídas em delineamento experimental inteiramente casualizado, com cinco tratamentos, seis repetições e quatro aves por unidade experimental. As rações experimentais foram formuladas a base de milho e farelo de soja e os tratamentos foram constituídos por cinco níveis de sódio (0,12; 0,17; 0,22; 0,27 e 0,32%) derivados do sal comum. Foram avaliados: consumo de ração (g), produção de ovos (%), peso dos ovos (g), massa de ovos (g), conversão alimentar por massa (kg/kg) e por dúzia (kg/dz) de ovos, gravidade específica (g/cm3), espessura de casca (mm), elementos dos ovos, tais como, gema (g e %), albúmen (g e %) e casca (g e %), viabilidade (%) e variação do peso corporal (g) das aves. Não foi verificado efeito (P>0,05) dos níveis de sódio sobre o peso da gema (g) e porcentagem de albúmen. Houve efeito linear (P<0,01) decrescente para o consumo de ração, diminuindo o consumo à medida que aumentou o nível de sódio na ração. Verificou-se efeito quadrático (P<0,01) para a produção de ovos, peso dos ovos, conversão alimentar por massa e dúzia de ovos, pesos de albúmen e casca, gravidade específica, espessura de casca e porcentagem de casca e gema (P<0,05). Os níveis de sódio analisados influenciaram os componentes dos ovos e o desempenho zootécnico das aves acima de 0,27%, negativamente. Recomenda-se a inclusão de 0,20% de sódio na ração de poedeiras semipesadas criadas em clima quente, após o pico de postura, para proporcionar melhor qualidade dos ovos e desempenho produtivo.

Efeito da categoria reprodutiva sobre a fertilidade de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento / Effect of mare status on fertility of inseminated mares with jackass semen diluted and cooled at 5ºC for 12 hours

Estudou-se o efeito da categoria reprodutiva sobre a fertilidade de éguas inseminadas com sêmen asinino diluído, resfriado e armazenado. Os ciclos foram acompanhados por palpação retal e rufiação, sendo as inseminações realizadas às terças, quintas e sábados, a partir da detecção de um folículo de 3,0 a 3,5cm de diâmetro, em um dos ovários, até a ovulação. O sêmen de cinco jumentos da raça Pêga foi diluído nos diluidores de leite em pó desnatado-glicose ou glicina-gema de ovo, resfriado a 5ºC e armazenado por 12 horas, sendo a dose inseminante de 400 x 106 espermatozoides móveis (no momento da diluição final, pré-resfriamento) depositada no corpo do útero. O diagnóstico de gestação foi realizado por meio de palpação transretal, rufiação e ultrassonografia, realizada a cada 14 dias. Os resultados de 195 ciclos, referentes a 141 éguas, foram agrupados de acordo com a categoria reprodutiva a que pertenciam: potra, égua solteira, égua parida e no "cio do potro". As taxas de concepção, ao primeiro ciclo, foram de 60,00%, 48,28%, 75,00% e 47,17% e, após quatro ciclos, de 61,54%, 47,13%, 54,76% e 47,17%, na mesma ordem para as categorias descritas anteriormente. A categoria reprodutiva não teve efeito (P>0,05) sobre a fertilidade das éguas inseminadas com sêmen asinino resfriado, sendo as potras, éguas solteiras, éguas paridas e no "cio do potro" igualmente eficientes para o uso na reprodução.(AU)

The effect of the mare status on fertility of mares inseminated with diluted, cooled and stored jackass semen, was studied. The cycles were controlled by transrectal palpation and teasing, and mares were inseminated every Tuesday, Thursday and Saturday, from the detection of a follicle with 3.0 to 3.5cm diameter in one of the ovaries until ovulation. The semen of five Pêga jackasses was diluted in skim milk-glucose or in egg yolk-glycine extender and cooled at 5ºC for 12 hours, with the inseminate dose of 400 x 106 motile spermatozoa (at the moment of the final dilution, before cooling). The inseminations were carried out in the uterine body. Pregnancy was detected using transrectal palpation, teasing and ultrasound exams every 14 days. The results of 195 cycles of 141 mares were grouped according to the mare status: maiden, barren, lactation or in foal heat. Pregnancy rates for the first cycle were 60.00%, 48.28%, 75.00% and 47.17%, and after four cycles, the pregnancy rates/cycle were 61.54%, 47.13%, 54.76% and 47.17%, respectively for maiden, barren, lactation and in foal heat mares (P>0,05). The mare status did not affect pregnancy rates of mares inseminated with diluted and cooled jackass semen and were efficient to use on reproduction.(AU)

Efeito da categoria reprodutiva sobre a fertilidade de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento / Effect of mare status on fertility of inseminated mares with jackass semen diluted and cooled at 5ºC for 12 hours

Estudou-se o efeito da categoria reprodutiva sobre a fertilidade de éguas inseminadas com sêmen asinino diluído, resfriado e armazenado. Os ciclos foram acompanhados por palpação retal e rufiação, sendo as inseminações realizadas às terças, quintas e sábados, a partir da detecção de um folículo de 3,0 a 3,5cm de diâmetro, em um dos ovários, até a ovulação. O sêmen de cinco jumentos da raça Pêga foi diluído nos diluidores de leite em pó desnatado-glicose ou glicina-gema de ovo, resfriado a 5ºC e armazenado por 12 horas, sendo a dose inseminante de 400 x 106 espermatozoides móveis (no momento da diluição final, pré-resfriamento) depositada no corpo do útero. O diagnóstico de gestação foi realizado por meio de palpação transretal, rufiação e ultrassonografia, realizada a cada 14 dias. Os resultados de 195 ciclos, referentes a 141 éguas, foram agrupados de acordo com a categoria reprodutiva a que pertenciam: potra, égua solteira, égua parida e no "cio do potro". As taxas de concepção, ao primeiro ciclo, foram de 60,00%, 48,28%, 75,00% e 47,17% e, após quatro ciclos, de 61,54%, 47,13%, 54,76% e 47,17%, na mesma ordem para as categorias descritas anteriormente. A categoria reprodutiva não teve efeito (P>0,05) sobre a fertilidade das éguas inseminadas com sêmen asinino resfriado, sendo as potras, éguas solteiras, éguas paridas e no "cio do potro" igualmente eficientes para o uso na reprodução...

The effect of the mare status on fertility of mares inseminated with diluted, cooled and stored jackass semen, was studied. The cycles were controlled by transrectal palpation and teasing, and mares were inseminated every Tuesday, Thursday and Saturday, from the detection of a follicle with 3.0 to 3.5cm diameter in one of the ovaries until ovulation. The semen of five Pêga jackasses was diluted in skim milk-glucose or in egg yolk-glycine extender and cooled at 5ºC for 12 hours, with the inseminate dose of 400 x 106 motile spermatozoa (at the moment of the final dilution, before cooling). The inseminations were carried out in the uterine body. Pregnancy was detected using transrectal palpation, teasing and ultrasound exams every 14 days. The results of 195 cycles of 141 mares were grouped according to the mare status: maiden, barren, lactation or in foal heat. Pregnancy rates for the first cycle were 60.00%, 48.28%, 75.00% and 47.17%, and after four cycles, the pregnancy rates/cycle were 61.54%, 47.13%, 54.76% and 47.17%, respectively for maiden, barren, lactation and in foal heat mares (P>0,05). The mare status did not affect pregnancy rates of mares inseminated with diluted and cooled jackass semen and were efficient to use on reproduction...

Efeito da idade sobre a fertilidade e perdas embrionárias de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento / Effect of age on fertility and embryonic loss of mares inseminated with jackass semen diluted and cooled at 5ºC for 12 hours

Estudou-se o efeito da idade sobre a fertilidade de éguas inseminadas com sêmen asinino diluído, resfriado e armazenado. Os ciclos foram acompanhados por palpação transretal e rufiação, sendo as inseminações realizadas às terças, quintas e sábados, a partir da detecção de um folículo de 3,0 a 3,5cm de diâmetro, em um dos ovários, até a ovulação. O sêmen de cinco jumentos da raça Pêga foi diluído nos diluidores de leite em pó desnatado-glicose ou glicina-gema de ovo, resfriado a 5ºC e armazenado por 12 horas, sendo a dose inseminante de 400 x 106 espermatozoides móveis (no momento da diluição final, pré-resfriamento). Os resultados de 195 ciclos estrais, referentes a 141 éguas, foram agrupados em classes, de acordo com a idade das éguas: 2,5 a 6 anos, 6,5 a 10 anos, 10,5 a 14 anos e 14,5 a 19 anos. As taxas de concepção, ao primeiro ciclo, foram de 68,42%, 50,75%, 46,88% e 52,17% e, após quatro ciclos, de 69,57%, 47,92%, 46,34% e 45,71% para as faixas etárias de 2,5 a 6, 6,5 a 10, 10,5 a 14 e 14,5 a 19 anos, respectivamente (P>0,05). A idade não teve efeito sobre a fertilidade das éguas inseminadas com sêmen asinino diluído e resfriado. (AU)

The effect of the mare age on fertility of mares inseminated with diluted, cooled and stored jackass semen was studied. The females were controlled by transrectal palpation and teasing, and inseminated every Tuesday, Thursday and Saturday, since the detection of a 3.0 to 3.5cm follicle diameter, in one of the ovaries, until ovulation. The semen of five Pêga jackasses was diluted in skim milk-glucose or in egg yolk-glicine extender and cooled at 5ºC for 12 hours, with the inseminate dose of 400 x 106 motile spermatozoa (at the moment of the final dilution, before cooling). The results of 195 cycles of 141 mares were grouped, in accordance with the age: 2.5 to 6 years, 6.5 to 10 years, 10.5 to 14 years and 14.5 to 19 years. The pregnancy rates for the first cycle were 68.42%, 50.75%, 46.88% and 52.17%, and after four cycles, the pregnancy rates/cycle were 69.57%, 47.92%, 46.34% and 45.71%, respectively for 2.5 to 6, 6.5 to 10, 10.5 to 14 and 14.5 to 19 years (P>0,05). The mare age had no influence on fertility, using diluted and cooled jackass semen.(AU)

Efeito da idade sobre a fertilidade e perdas embrionárias de éguas inseminadas com sêmen asinino diluído e resfriado a 5ºC por 12 horas de armazenamento / Effect of age on fertility and embryonic loss of mares inseminated with jackass semen diluted and cooled at 5ºC for 12 hours

Estudou-se o efeito da idade sobre a fertilidade de éguas inseminadas com sêmen asinino diluído, resfriado e armazenado. Os ciclos foram acompanhados por palpação transretal e rufiação, sendo as inseminações realizadas às terças, quintas e sábados, a partir da detecção de um folículo de 3,0 a 3,5cm de diâmetro, em um dos ovários, até a ovulação. O sêmen de cinco jumentos da raça Pêga foi diluído nos diluidores de leite em pó desnatado-glicose ou glicina-gema de ovo, resfriado a 5ºC e armazenado por 12 horas, sendo a dose inseminante de 400 x 106 espermatozoides móveis (no momento da diluição final, pré-resfriamento). Os resultados de 195 ciclos estrais, referentes a 141 éguas, foram agrupados em classes, de acordo com a idade das éguas: 2,5 a 6 anos, 6,5 a 10 anos, 10,5 a 14 anos e 14,5 a 19 anos. As taxas de concepção, ao primeiro ciclo, foram de 68,42%, 50,75%, 46,88% e 52,17% e, após quatro ciclos, de 69,57%, 47,92%, 46,34% e 45,71% para as faixas etárias de 2,5 a 6, 6,5 a 10, 10,5 a 14 e 14,5 a 19 anos, respectivamente (P>0,05). A idade não teve efeito sobre a fertilidade das éguas inseminadas com sêmen asinino diluído e resfriado...

The effect of the mare age on fertility of mares inseminated with diluted, cooled and stored jackass semen was studied. The females were controlled by transrectal palpation and teasing, and inseminated every Tuesday, Thursday and Saturday, since the detection of a 3.0 to 3.5cm follicle diameter, in one of the ovaries, until ovulation. The semen of five Pêga jackasses was diluted in skim milk-glucose or in egg yolk-glicine extender and cooled at 5ºC for 12 hours, with the inseminate dose of 400 x 106 motile spermatozoa (at the moment of the final dilution, before cooling). The results of 195 cycles of 141 mares were grouped, in accordance with the age: 2.5 to 6 years, 6.5 to 10 years, 10.5 to 14 years and 14.5 to 19 years. The pregnancy rates for the first cycle were 68.42%, 50.75%, 46.88% and 52.17%, and after four cycles, the pregnancy rates/cycle were 69.57%, 47.92%, 46.34% and 45.71%, respectively for 2.5 to 6, 6.5 to 10, 10.5 to 14 and 14.5 to 19 years (P>0,05). The mare age had no influence on fertility, using diluted and cooled jackass semen...

Meningoencefalite por Herpesvirus bovino 5 em Minas Gerais: relato de caso clínico / Meningoencephalitis by Bovine herpesvirus 5 in Minas Gerais state: clinical case report

Relata-se um caso de meningoencefalite causada por Herpesvirus bovino 5 (BoHV-5) heritabilityem uma vaca com cinco anos de idade. O animal manifestou quadro clínico inicial de síndrome medular baixa, caracterizada por incoordenação dos membros pélvicos, sinais estes ainda não descritos para a enfermidade. Dentro de pouco tempo a doença evoluiu para síndrome cerebral, e o óbito ocorreu seis dias após o inicio dos sintomas. Na histopatologia, evidenciou-se meningoencefalite difusa, não supurada, e a confirmação do diagnóstico foi feita por reação em cadeia de polimerase e sequenciamento do segmento parcial da glicoproteína G do vírus. O trabalho confirma a presença do BoHV-5 em Minas Gerais, descreve características clínicas novas para a enfermidade e ressalta sua importância no diagnóstico diferencial das neuropatias bovinas.(AU)

A clinical case of meningoencephalitis by Bovine herpesvirus 5 (BoHV-5) in a five-year-old cow was reported. The disease began with low spinal cord signs, characterized by incoordination, and these symptoms had never been related to this illness before. Signs of a brain syndrome were observed and the cow died in six days. At the histopathology, a spread non-supurative meningoencephalitis was diagnosed, and the virus identification was made by PCR and partial sequence of the glycoprotein G. This study confirm the BoHV-5 presence in the State of Minas Gerais, Brazil, describes new clinic characteristics, and show the importance of the disease in the differentiate diagnosis with others bovine central nervous system affections.(AU)

Meningoencefalite por Herpesvirus bovino 5 em Minas Gerais: relato de caso clínico / Meningoencephalitis by Bovine herpesvirus 5 in Minas Gerais state: clinical case report

Relata-se um caso de meningoencefalite causada por Herpesvirus bovino 5 (BoHV-5) heritabilityem uma vaca com cinco anos de idade. O animal manifestou quadro clínico inicial de síndrome medular baixa, caracterizada por incoordenação dos membros pélvicos, sinais estes ainda não descritos para a enfermidade. Dentro de pouco tempo a doença evoluiu para síndrome cerebral, e o óbito ocorreu seis dias após o inicio dos sintomas. Na histopatologia, evidenciou-se meningoencefalite difusa, não supurada, e a confirmação do diagnóstico foi feita por reação em cadeia de polimerase e sequenciamento do segmento parcial da glicoproteína G do vírus. O trabalho confirma a presença do BoHV-5 em Minas Gerais, descreve características clínicas novas para a enfermidade e ressalta sua importância no diagnóstico diferencial das neuropatias bovinas.

A clinical case of meningoencephalitis by Bovine herpesvirus 5 (BoHV-5) in a five-year-old cow was reported. The disease began with low spinal cord signs, characterized by incoordination, and these symptoms had never been related to this illness before. Signs of a brain syndrome were observed and the cow died in six days. At the histopathology, a spread non-supurative meningoencephalitis was diagnosed, and the virus identification was made by PCR and partial sequence of the glycoprotein G. This study confirm the BoHV-5 presence in the State of Minas Gerais, Brazil, describes new clinic characteristics, and show the importance of the disease in the differentiate diagnosis with others bovine central nervous system affections.

[Effect of neuromuscular electrical stimulation and isotonic exercises in flexor and extensor muscles of knee of hemiplegic patients]. / Efecto de la estimulación eléctrica neuromuscular y de los ejercicios isotónicos en los músculos flexores y extensores de la rodilla en pacientes hémiplejicos.

AIM: To verify the muscular force and resistance to the movement of the flexor and extensor muscles of the knee of patients with spasticity after treatment with neuromuscular electrical stimulation (NMES) and isotonic exercises. PATIENTS AND METHODS: The patients this study were divided into group 1 (NMES) and group 2 (isotonic exercises). Their muscular torque and resistance to the movement of the flexor and extensor knee muscles were measured by the isokinetic dynamometer and the degree of spasticity by the modified Ashworth scale before and after ten sessions. RESULTS: Alterations in the scores of the modified Ashworth scale were not observed. An increase in the flexor torque in group 1 (p = 0.041) and in group 2 (p = 0.001) was verified. In the passive mode, group 1 presented a reduction of resistance to the flexion movement (p = 0.026), while in group 2, a reduction of resistance to both the flexion (p = 0,029) and extension movements (p = 0.019) was verified. CONCLUSIONS: The two therapeutical resources had their efficiency proven only for the increase of the force of the flexor muscles. The resistance to movement, the isotonic exercises were more effective because they promoted a reduction in the resistance of the flexor and extensor knee muscles.

Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation.

Ginger, the rhizome of Zingiber officinalis Roscoe (Zingiberaceae), is a common constituent of diets around the world and its extracts have been reported to exhibit several pharmacological activities. We investigated the effect of crude hydroalcoholic extract of ginger on the rat trachea hyperreactivity (RTHR) and lung inflammation induced by lipopolysaccharide (LPS). Our results demonstrate that ginger extract and celecoxib attenuated RTHR 90 min and 48 h after LPS. Ginger and celecoxib reduced the serum level of prostaglandin (PGE2) and thromboxane (TXA2) 90 min after LPS. Celecoxib and ginger also reduced myeloperoxidase activity and the number of cells in rat bronchoalveolar lavage 48 h post-LPS. On lung parenchyma, ginger and celecoxib reduced the release of PGE2 and TXA2 48 h post-LPS. These results suggest that ginger exerts an anti-inflammatory effect on lung attenuating RTHR and COX metabolites seem to be involved in these processes.

Effect of low-level laser therapy on hemorrhagic lesions induced by immune complex in rat lungs.

OBJECTIVE: The aim of this study was to investigate if low-level laser therapy (LLLT) can modulate formation of hemorrhagic lesions induced by immune complex. BACKGROUND DATA: There is a lack of information on LLLT effects in hemorrhagic injuries of high perfusion organs, and the relative efficacy of LLLT compared to anti-inflammatory drugs. METHODS: A controlled animal study was undertaken with 49 male Wistar rats randomly divided into seven groups. Bovine serum albumin (BSA) i.v. was injected through the trachea to induce an immune complex lung injury. The study compared the effect of irradiation by a 650-nm Ga-Al-As laser with LLLT doses of 2.6 Joules/cm(2) to celecoxib, dexamethasone, and control groups for hemorrhagic index (HI) and myeloperoxide activity (MPO) at 24 h after injury. RESULTS: The HI for the control group was 4.0 (95% CI, 3.7-4.3). Celecoxib, LLLT, and dexamethasone all induced significantly (p < 0.01) lower HI than control animals at 2.5 (95% CI, 1.9-3.1), 1.8 (95% CI, 1.2-2.4), and 1.5 (95% CI, 0.9-2.1), respectively, for all comparisons to control. Dexamethasone, but not celecoxib, induced a slightly, but significantly lower HI than LLLT (p = 0.04). MPO activity was significantly decreased in groups receiving celecoxib at 0.87 (95% CI, 0.63-1.11), dexamethasone at 0.50 (95% CI, 0.24-0.76), and LLLT at 0.7 (95% CI, 0.44-0.96) when compared to the control group, at 1.6 (95% CI, 1.34-1.96; p < 0.01), but there were no significant differences between any of the active treatments. CONCLUSION: LLLT at a dose of 2.6 Joules/cm(2) induces a reduction of HI levels and MPO activity in hemorrhagic injury that is not significantly different from celecoxib. Dexamethasone is slightly more effective than LLLT in reducing HI, but not MPO activity.