RESUMO
Developing novel antimicrobial wound dressings that have the potential to address the challenges associated with chronic wounds is highly imperative in providing effective infection control and wound healing support. Biocompatible electrospun nanofibers with their high porosity and surface area enabling efficient drug loading and delivery have been investigated in this regard as viable candidates for chronic wound care. Here, we design Casein/Polyvinyl alcohol (CAN/PVA) nanofibers reinforced with silver nanoparticles (Ag NPs) by the electrospinning technique to develop diabetic wound healing scaffolds. The prepared samples were characterized using spectroscopic and electron microscopic techniques. The biocompatibility of the polymer samples were assessed using 3 T3 fibroblast cell lines and the maximum cell viability was found to 95 % at a concentration of 50 µg/mL for the prepared nanofibers. Scratch assay tests were also performed to analyze the wound healing activity of the nanofibers wherein they demonstrated increased migration and proliferation of fibroblast 3 T3 cells. Moreover, these nanofibers also exhibit antibacterial efficiency against Gram-negative bacteria, Escherichia coli (E.coli). Therefore, the antimicrobial nature of the electrospun nanofibers coupled with their moisture absorption properties and wound healing ability render them as effective materials for wound dressing applications.
Assuntos
Antibacterianos , Caseínas , Nanopartículas Metálicas , Nanofibras , Álcool de Polivinil , Prata , Engenharia Tecidual , Alicerces Teciduais , Nanofibras/química , Álcool de Polivinil/química , Prata/química , Prata/farmacologia , Nanopartículas Metálicas/química , Alicerces Teciduais/química , Engenharia Tecidual/métodos , Caseínas/química , Caseínas/farmacologia , Animais , Antibacterianos/farmacologia , Antibacterianos/química , Camundongos , Escherichia coli/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Proliferação de Células/efeitos dos fármacosRESUMO
The title compound, C(30)H(44)N(2)O(4), was obtained from the dimerization of 4-hexyl-oxyvanillin with ethyl-enediamine in 95% methanol solution. It adopts a trans configuration with respect to the C=N bond and possesses a crystallographically imposed centre of symmetry.
RESUMO
Black, Hispanic, and white mothers (N = 60) of young children with disabilities were interviewed to explore their feelings and reactions to their child. Trends reveal that Hispanic mothers reported an attitude of self-sacrifice toward the child and greater spousal denial of the disability more often than did the other mothers. Stages of reaction from strong negative feelings to later periods of adjustment were most often reported by both Hispanic and white subjects. Although severity of retardation was not predictive of parental reporting of stages, parents of children who received a diagnosis within a month of birth were more likely to report subsequent adjustment stages.
