RESUMO
Tomato (Solanum lycopersicum L., Solanaceae) is an excellent model plant for genomic research of solanaceous plants, as well as for studying the development, ripening, and metabolism of fruit. In 2003, the International Solanaceae Project (SOL, www.sgn.cornell.edu ) was initiated by members from more than 30 countries, and the tomato genome-sequencing project is currently underway. Genome sequence of tomato obtained by this project will provide a firm foundation for forthcoming genomic studies such as the comparative analysis of genes conserved among the Solanaceae species and the elucidation of the functions of unknown tomato genes. To exploit the wealth of the genome sequence information, there is an urgent need for novel resources and analytical tools for tomato functional genomics. Here, we present an overview of the development of genetic and genomic resources of tomato in the last decade, with a special focus on the activities of Japan SOL and the National Bio-Resource Project in the development of functional genomic resources of a model cultivar, Micro-Tom.
RESUMO
The induction of alpha-amylase triggered by gibberellic acid in barley embryos is repressed by sugars. We investigated the effects of glucose on the gibberellin signal transduction pathway to localize the site of interaction of the sugar/hormone signalling pathways. Our results indicate that glucose represses gibberellin signalling late along this hormone transduction pathway, downstream of transcription of the gibberellin-modulated transcriptional activator (GAMYB) needed for alpha-amylase induction. This result suggests either that glucose repression is transduced by a pathway independent of gibberellin signalling or that repression occurs at the level of GAMYB translation.
Assuntos
Glucose/farmacologia , Hordeum/efeitos dos fármacos , Fatores de Transcrição/genética , alfa-Amilases/genética , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Giberelinas/farmacologia , Hordeum/embriologia , Hordeum/enzimologia , Hibridização In Situ , RNA de Plantas/efeitos dos fármacos , RNA de Plantas/genética , RNA de Plantas/metabolismo , Sementes/efeitos dos fármacos , Sementes/genética , Transcrição Gênica/efeitos dos fármacosRESUMO
We investigated sugar uptake and transport in rice (Oryza sativa) embryo during grain germination. Endogenous sugar levels, accumulation of starch granules, and gene expression of a rice sucrose transporter (OsSUT1) were examined using rice embryos germinated with or without exogenous sugar supply. Starch granules remarkably accumulated in the cells around vascular bundles as a consequence of the sugar taken up by the embryos, indicating that the taken-up sugars are transiently converted into starch. In situ detection for OsSUT1 mRNA indicated its localization in the phloem companion cells. Furthermore, northern-blot and in situ hybridization analyses showed that OsSUT1 expression is not detectable in embryos subjected to sugar starvation conditions, whereas its expression is enhanced by an increased endogenous sugar level. Overall results indicate that the expression of companion cell-specific sucrose transporter, OsSUT1 is regulated by the endogenous sugar status as well as light exposure.
Assuntos
Metabolismo dos Carboidratos , Proteínas de Transporte/metabolismo , Germinação , Luz , Proteínas de Membrana Transportadoras , Oryza/genética , Proteínas de Plantas/metabolismo , Transporte Biológico , Northern Blotting , Proteínas de Transporte/genética , Regulação da Expressão Gênica de Plantas , Hibridização In Situ , Oryza/fisiologia , Oryza/ultraestrutura , Proteínas de Plantas/genética , Estruturas Vegetais/metabolismo , Estruturas Vegetais/ultraestrutura , Sementes/fisiologia , Sementes/ultraestrutura , Amido/metabolismoRESUMO
Increasing evidence shows that sugars can act as signals affecting plant metabolism and development. Some of the effects of sugars on plant growth and development suggest an interaction of sugar signals with hormonal regulation. We investigated the effects of sugars on the induction of [alpha]-amylase by gibberellic acid in barley embryos and aleurone layers. Our results show that sugar and hormonal signaling interact in the regulation of gibberellic acid-induced gene expression in barley grains. The induction of [alpha]-amylase by gibberellic acid in the aleurone layer is unaffected by the presence of sugars, but repression by carbohydrates is effective in the embryo. [alpha]-Amylase expression in the embryo is localized to the scutellar epithelium and is hormone and sugar modulated. The effects of glucose are independent from the effects of sugars on gibberellin biosynthesis. They are not due to an osmotic effect, they are independent of abscisic acid, and only hexokinase-phosphorylatable glucose analogs are able to trigger gene repression. Overall, the results suggest the existence of an interaction between the hormonal and metabolic regulation of [alpha]-amylase genes in barley grains.