RESUMO
Immature or reticulated platelets are known as a clinical marker of thrombopoiesis. Recently, an automatic method was established to detect reticulated platelets as immature platelet fraction (IPF) by means of hematology analyzer XE-2100. We assessed the effects of IPF detection after chemotherapy for various pediatric malignant disorders of 16 patients. Our results indicate that IPF should be considered a useful marker of imminent platelet recovery so that unnecessary platelet transfusion can be avoided.
Assuntos
Plaquetas/citologia , Transfusão de Plaquetas/métodos , Adolescente , Automação , Plaquetas/metabolismo , Criança , Pré-Escolar , Desenho de Equipamento , Humanos , Lactente , Recém-Nascido , Contagem de Plaquetas , Valores de Referência , Transplante de Células-Tronco , Trombopoese , Fatores de TempoRESUMO
We taught fourth-year students in a university about patient education using the diabetes retinopathy model in adult nursing practice. We analyzed teacher's student guidance plans and the guidance process using a clinical training education analysis form from the viewpoint of qualitative interpretation. We evaluated the degree of achievement of the students' goal for practice and the training subject. Consequently, the teacher developed training guidance as a class.
Assuntos
Retinopatia Diabética/enfermagem , Bacharelado em Enfermagem/métodos , Adulto , Humanos , Japão , Modelos AnatômicosRESUMO
We evaluated the effects of magnetic resonance (MR) imaging on human neutrophil functions. In addition of the counting of leukocyte and neutrophil number, expression levels of adhesion molecules on neutrophil surface such as CD11b and L-selectin, and reactive oxygen species (ROS) production were determined by means of flowcytometry. Complete blood count did not show any difference between before and after MR imaging in five normal healthy volunteers. The levels of cell surface adhesion molecules were not altered in both in vivo MR imaging (n=5) and in vitro MR exposure experiments (n=13). Moreover, the levels of ROS production were also not affected by in vivo MR imaging. On the other hand, neutrophils exposed to MR in vitro exhibited significant increase in ROS production after stimulation with fMLP combined with lipopolysaccharide, although no increase was observed with PMA stimulation. Actually, there have been no reports describing the complication relating to hyper-neutrophil function as far as we could search, but it might be necessary to evaluate the biological effects of MR imaging especially under the pathologic circumstances that induce neutrophil activation.
