RESUMO
We have designed an optimum holographic optical element (HOE) lens recorded by visible laser beams for an infrared two-dimensional vertical-cavity surface-emitting laser (VCSEL) array. The hologram computer-aided design tool that we have developed is used for the optimum design. The optimum HOE has both high light efficiency and a small amount of aberration. An Nd:YAG laser operated at 532 nm and an 8 x 8 VCSEL array operated at 850 nm are used for HOE recording and reconstruction, respectively. The designed lens is experimentally demonstrated, and the experimental results of the lens almost agree with the numerical results.
RESUMO
We have developed a novel method for cloning gene family members by using a polymerase chain reaction technique. The method is based on the amplification of a broad range of homologous genes in combination with the specific inhibition of already cloned genes. To accomplish this, we designed degenerate primers to highly conserved regions among the gene family members, and inhibitory primers to the divergent region at the 3'-margin of each degenerate primer. The 5'-end of the inhibitory primer, the 3'-end of which was aminated, had 3-4 bases overlapping the 3'-end of the degenerate primer. The potential of this method was demonstrated by the successful cloning of a novel member of the yeast MKC7/YAP3 gene family homologue from a filamentous fungus, Aspergillus oryzae, by inhibiting amplification of an already cloned homologue, opsB.
