The effect of intensified illuminance and artificial light at night on fitness and susceptibility to abiotic and biotic stressors.

Changing light conditions due to human activities represents an important emerging environmental concern. Although changes to natural light conditions can be independently detrimental, in nature, organisms commonly face multiple stressors. To understand the consequences of altered light conditions, we exposed a model amphibian (wood frog; Lithobates sylvaticus) to a control and two anthropogenic light conditions: intensified daytime illuminance and artificial light at night - ALAN (intensified daytime illuminance + extended photoperiod). We measured (1) metrics of fitness (hatching success as well as survival to, size at, and time to metamorphosis) (2) susceptibility (time to death) to a commonly co-occurring anthropogenic stressor, road salt (NaCl) and (3) susceptibility (infection load) to a common parasite (trematode). We also explored behavioral (swimming activity) and physiological (baseline corticosterone (CORT) release rates) changes induced by these light conditions, which may mediate changes in the other measured parameters. We found that both intensified daytime illuminance and ALAN reduced hatching success. In contrast, for amphibians that successfully hatched, neither treatment affected amphibian survival or time to metamorphosis but individuals exposed to ALAN were larger at metamorphosis. The light treatments also had marginal effects; individuals in ALAN treatments were more susceptible to NaCl and trematodes. Finally, tadpoles exposed to ALAN moved significantly less than tadpoles in the control and intensified daytime illuminance treatments, while light had no effect on CORT release rate. Overall, changes in light conditions, in particular ALAN, significantly impacted an amphibian model in laboratory conditions. This work underscores the importance of considering not only the direct effects of light on fitness metrics but also the indirect effects of light with other abiotic and biotic stressors. Anthropogenic-induced changes to light conditions are expected to continue increasing over time so understanding the diverse consequences of shifting light conditions will be paramount to protecting wildlife populations.

The effects of different cold-temperature regimes on development, growth, and susceptibility to an abiotic and biotic stressor.

Global climate change is expected to both increase average temperatures as well as temperature variability.Increased average temperatures have led to earlier breeding in many spring-breeding organisms. However, individuals breeding earlier will also face increased temperature fluctuations, including exposure to potentially harmful cold-temperature regimes during early developmental stages.Using a model spring-breeding amphibian, we investigated how embryonic exposure to different cold-temperature regimes (control, cold-pulse, and cold-press) affected (a) compensatory larval development and growth, (b) larval susceptibility to a common contaminant, and (c) larval susceptibility to parasites.We found: (a) no evidence of compensatory development or growth, (b) larvae exposed to the cold-press treatment were more susceptible to NaCl at 4-days post-hatching but recovered by 17-days post-hatching, and (c) larvae exposed to both cold treatments were less susceptible to parasites.These results demonstrate that variation in cold-temperature regimes can lead to unique direct and indirect effects on larval growth, development, and response to stressors. This underscores the importance of considering cold-temperature variability and not just increased average temperatures when examining the impacts of climate disruption.