Temperature dependence of the viscoelastic properties of a natural gastropod mucus by Brillouin light scattering spectroscopy.

Brillouin spectroscopy was used to probe the viscoelastic properties of a natural gastropod mucus at GHz frequencies over the range -11 °C ≤ T ≤ 52 °C. Anomalies in the temperature dependence of mucus longitudinal acoustic mode peak parameters and associated viscoelastic properties at T = -2.5 °C, together with the appearance of a peak due to ice at this temperature, suggest that the mucus undergoes a phase transition from a viscous liquid state to one in which liquid mucus and solid ice phases coexist. Failure of this transition to proceed to completion even at -11 °C is attributed to glycoprotein-water interaction. The temperature dependence of the viscoelastic properties and the phase behaviour suggest that water molecules bind to glycoprotein at a temperature above the onset of freezing and that the reduced ability of this bound water to take on a configuration that facilitates freezing is responsible for the observed freezing point depression and gradual nature of the liquid-solid transition.

Resonance spectroscopy for solids with layers of different materials.

A powerful method for measuring the elastic properties of solid materials is resonance spectroscopy. An advantage of this method is that the experimental measurement is relatively easy, and the difficult analysis is accomplished with a computer program. A particular computer algorithm has become standard because it is fast and may be applied to a wide range of shapes of solid samples. However, the algorithm does not work well for solids with layers of different materials. Fortunately, a relatively easy modification of the customary computer algorithm enables it to work for layered solids.

Increasing COVID-19 vaccination in the United States: projected impact on cases, hospitalizations, and deaths by age and racial group.

OBJECTIVES: Minority populations in the United States face a disproportionate burden of illness from COVID-19 infection and have lower vaccination rates compared with other groups. This study estimated the equity implications of increased COVID-19 vaccination in the United States, with a focus on the number of cases, hospitalizations, and deaths avoided. STUDY DESIGN: This was an observational real-world modeling study. METHODS: Data from the Centers for Disease Control and Prevention (CDC) were used to identify the remaining unvaccinated US population by county, age, and race as of October 22, 2021. The number of COVID-19 cases, hospitalizations, and deaths avoided were calculated based on case incidence and death data from the CDC, along with data on race- and age-specific hospitalization multipliers, under a scenario in which half of the remaining unvaccinated population per county, race, and age group obtained a full vaccine regimen. RESULTS: Vaccinating half of the remaining unvaccinated population in each age and race subgroup within counties would result in an estimated 22.09 million COVID-19 cases avoided, 1.38 million hospitalizations avoided, and 150,000 deaths avoided over 12 months. Some minority groups, particularly Black and Hispanic/Latino populations, were projected to experience substantial benefits from increased vaccination rates as they face both lower vaccination rates and worse outcomes if infected with COVID-19. CONCLUSIONS: Increasing COVID-19 vaccination in the United States not only benefits the population as a whole but also serves as a potentially useful lever to reduce the disproportionate burden of COVID-19 illness among minority populations.

Regional and Volumetric Parameters for Diffusion-Weighted WHO Grade II and III Glioma Genotyping: A Method Comparison.

BACKGROUND AND PURPOSE: Studies consistently report lower ADC values in isocitrate dehydrogenase (IDH) wild-type gliomas than in IDH mutant tumors, but their methods and thresholds vary. This research aimed to compare volumetric and regional ADC measurement techniques for glioma genotyping, with a focus on IDH status prediction. MATERIALS AND METHODS: Treatment-naïve World Health Organization grade II and III gliomas were analyzed by 3 neuroradiologist readers blinded to tissue results. ADC minimum and mean ROIs were defined in tumor and in normal-appearing white matter to calculate normalized values. T2-weighted tumor VOIs were registered to ADC maps with histogram parameters (mean, 2nd and 5th percentiles) extracted. Nonparametric testing (eta2 and ANOVA) was performed to identify associations between ADC metrics and glioma genotypes. Logistic regression was used to probe the ability of VOI and ROI metrics to predict IDH status. RESULTS: The study included 283 patients with 79 IDH wild-type and 204 IDH mutant gliomas. Across the study population, IDH status was most accurately predicted by ROI mean normalized ADC and VOI mean normalized ADC, with areas under the curve of 0.83 and 0.82, respectively. The results for ROI-based genotyping of nonenhancing and solid-patchy enhancing gliomas were comparable with volumetric parameters (area under the curve = 0.81-0.84). In rim-enhancing, centrally necrotic tumors (n = 23), only volumetric measurements were predictive (0.90). CONCLUSIONS: Regional normalized mean ADC measurements are noninferior to volumetric segmentation for defining solid glioma IDH status. Partially necrotic, rim-enhancing tumors are unsuitable for ROI assessment and may benefit from volumetric ADC quantification.

Using resonance to measure the independent elastic constants of problematic materials such as elastomers.

The determination of the elastic constants for a solid material may be conveniently accomplished by measuring resonances corresponding to the elastic normal modes of a sample. Isotropic materials should be particularly straightforward, since they involve only two independent elastic constants, the shear modulus and the longitudinal modulus. In practice one typically measures the shear modulus and Young's modulus, but for some problematic materials these are not truly independent. Probing the microscopic processes of solid mechanical behavior requires knowledge of independent moduli. In this paper, a resonance method for directly measuring the independent moduli for problematic materials is described.

Eigenfunction orthogonality for one-dimensional acoustic systems with interior or end point conditions.

Some common exercises presented in introductory acoustics courses and texts illustrate solutions involving eigenvalues and eigenfunctions. Challenging extensions of these, even for one-dimensional (1D) systems, might involve a mass or spring loading the acoustic medium at an end point or at an interior point. These problems might be extended further by requiring that some given function be expanded in a series of the eigenfunctions, but such extended problems may lead to unexpected complications in regard to eigenfunction orthogonality. In this paper, Sturm-Liouville theory is used to develop a systematic method for predetermining eigenfunction orthogonality for 1D systems loaded at end points or interior points or having properties that change with jump discontinuities.

Lessons learned from the development of a hidradenitis suppurativa xenograft mouse model.

Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease originating from the pilosebaceous unit, in which patients develop painful abscesses, sinus tracts, nodules and scarring, typically in intertriginous areas. Major gaps in our understanding of HS exist, and these may be partially due to the lack of an animal model for experimental studies. We developed an HS xenograft mouse model using human HS lesions grafted onto immunocompromised mice. Although the model had its limitations, several informative lessons were learned, which may contribute to future attempts at an HS animal model.

A paleosol record of the evolution of Cr redox cycling and evidence for an increase in atmospheric oxygen during the Neoproterozoic.

Atmospheric oxygen levels control the oxidative side of key biogeochemical cycles and place limits on the development of high-energy metabolisms. Understanding Earth's oxygenation is thus critical to developing a clearer picture of Earth's long-term evolution. However, there is currently vigorous debate about even basic aspects of the timing and pattern of the rise of oxygen. Chemical weathering in the terrestrial environment occurs in contact with the atmosphere, making paleosols potentially ideal archives to track the history of atmospheric O2 levels. Here we present stable chromium isotope data from multiple paleosols that offer snapshots of Earth surface conditions over the last three billion years. The results indicate a secular shift in the oxidative capacity of Earth's surface in the Neoproterozoic and suggest low atmospheric oxygen levels (<1% PAL pO2 ) through the majority of Earth's history. The paleosol record also shows that localized Cr oxidation may have begun as early as the Archean, but efficient, modern-like transport of hexavalent Cr under an O2 -rich atmosphere did not become common until the Neoproterozoic.

Apparent diffusion coefficient for molecular subtyping of non-gadolinium-enhancing WHO grade II/III glioma: volumetric segmentation versus two-dimensional region of interest analysis.

OBJECTIVES: To investigate if quantitative apparent diffusion coefficient (ADC) measurements can predict genetic subtypes of non-gadolinium-enhancing gliomas, comparing whole tumour against single slice analysis. METHODS: Volumetric T2-derived masks of 44 gliomas were co-registered to ADC maps with ADC mean (ADCmean) calculated. For the slice analysis, two observers placed regions of interest in the largest tumour cross-section. The ratio (ADCratio) between ADCmean in the tumour and normal appearing white matter was calculated for both methods. RESULTS: Isocitrate dehydrogenase (IDH) wild-type gliomas showed the lowest ADC values throughout (p < 0.001). ADCmean in the IDH-mutant 1p19q intact group was significantly higher than in the IDH-mutant 1p19q co-deleted group (p < 0.01). A volumetric ADCmean threshold of 1201 × 10-6 mm2/s identified IDH wild-type with a sensitivity of 83% and a specificity of 86%; a volumetric ADCratio cut-off value of 1.65 provided a sensitivity of 80% and a specificity of 92% (area under the curve (AUC) 0.9-0.94). A slice ADCratio threshold for observer 1 (observer 2) of 1.76 (1.83) provided a sensitivity of 80% (86%), specificity of 91% (100%) and AUC of 0.95 (0.96). The intraclass correlation coefficient was excellent (0.98). CONCLUSIONS: ADC measurements can support the distinction of glioma subtypes. Volumetric and two-dimensional measurements yielded similar results in this study. KEY POINTS: • Diffusion-weighted MRI aids the identification of non-gadolinium-enhancing malignant gliomas • ADC measurements may permit non-gadolinium-enhancing glioma molecular subtyping • IDH wild-type gliomas have lower ADC values than IDH-mutant tumours • Single cross-section and volumetric ADC measurements yielded comparable results in this study.

Organic and Inorganic Pollutant Concentrations Suggest Anthropogenic Contamination of Soils Along the Manali-Leh Highway, Northwestern Himalaya, India.

Most studies on roadside soil pollution have been performed in areas where petrol is the main fuel. Very little work has been conducted in regions where diesel predominates. We collected soil samples from four sites that span a precipitation gradient along the Manali-Leh Highway in northwestern Himalaya, India. This road traverses rough terrain and most of the vehicles that travel along it are diesel-driven. At each site, we collected samples at incremental distances from the highway (0, 2, 5, 10, 20, and 150 m), and at each distance we collected samples from three depths (3, 9, and 15 cm). We assessed the concentrations of 10 heavy metals (Al, Fe, Cr, Cu, Pb, Ni, Co, Zn, V, and Ba), total sulphur, and total organic carbon (TOC) at each distance, and we measured the concentration of 16 polycyclic aromatic hydrocarbons (PAHs) at 2 m from the highway. Overall, we found that metal concentrations are low and there is no relationship between concentrations and distance from the highway, or depth within the soil profile. Sulphur concentrations, on the other hand, are high in roadside soils and there is a negative relationship between concentration and distance from the highway. PAH concentrations are low, but the proportion of different ringed species suggests that their source is anthropogenic. Correlations between TOC and the various pollutants further suggest that diesel vehicles and potentially biomass combustion are starting to affect the roadside environment in remote northwestern India. We suggest that pollutant concentrations be regularly monitored.

Capturing Spatial Variability of Biogeochemical Mass Exchanges and Reaction Rates in Wetland Water and Soil through Model Compartmentalization.

A common phenomenon observed in natural and constructed wetlands is short-circuiting of flow and formation of stagnant zones that are only indirectly connected with the incoming water. Biogeochemistry of passive areas is potentially much different than that of active zones. In the research reported in this paper, the spatial resolution of a previously developed wetland nutrient cycling model was improved in order to capture the spatial variability of concentrations and reaction rates regarding nitrogen and carbon cycles throughout active and passive zones of wetlands. The upgraded model allows for several compartments in the horizontal domain, with all neighboring compartments connected through advective and dispersive/diffusive mass transport. The model was applied to data collected from a restored wetland in California that was characterized by the formation of a large stagnant zone at the southern end of the wetland due to close vicinity of the inlet and outlet structures in the northern end. Mass balance analysis revealed that over the course of the research period, about 23.4±3.9% of the incoming total nitrogen load was removed or retained by the wetland. It was observed that mass of all exchanges (physical and biogeochemical) regarding nitrogen cycling decreased along the activity gradient from active to passive zones. Model results also revealed that anaerobic processes become more significant along the activity gradient towards passive areas.

Manganese deposition in drinking water distribution systems.

This study provides a physicochemical assessment of manganese deposits on brass and lead components from two fully operational drinking water distributions systems. One of the systems was maintained with chlorine; the other, with secondary chloramine disinfection. Synchrotron-based in-situ micro X-ray adsorption near edge structure was used to assess the mineralogy. In-situ micro X-ray fluorescence mapping was used to demonstrate the spatial relationships between manganese and potentially toxic adsorbed metal ions. The Mn deposits ranged in thickness from 0.01 to 400 µm. They were composed primarily of Mn oxides/oxhydroxides, birnessite (Mn(3+) and Mn(4+)) and hollandite (Mn(2+) and Mn(4+)), and a Mn silicate, braunite (Mn(2+) and Mn(4+)), in varying proportions. Iron, chromium, and strontium, in addition to the alloying elements lead and copper, were co-located within manganese deposits. With the exception of iron, all are related to specific health issues and are of concern to the U.S. Environmental Protection Agency (U.S. EPA). The specific properties of Mn deposits, i.e., adsorption of metals ions, oxidation of metal ions and resuspension are discussed with respect to their influence on drinking water quality.

Rapid survey protocol that provides dynamic information on reef condition to managers of the Great Barrier Reef.

Managing to support coral reef resilience as the climate changes requires strategic and responsive actions that reduce anthropogenic stress. Managers can only target and tailor these actions if they regularly receive information on system condition and impact severity. In large coral reef areas like the Great Barrier Reef Marine Park (GBRMP), acquiring condition and impact data with good spatial and temporal coverage requires using a large network of observers. Here, we describe the result of ~10 years of evolving and refining participatory monitoring programs used in the GBR that have rangers, tourism operators and members of the public as observers. Participants complete Reef Health and Impact Surveys (RHIS) using a protocol that meets coral reef managers' needs for up-to-date information on the following: benthic community composition, reef condition and impacts including coral diseases, damage, predation and the presence of rubbish. Training programs ensure that the information gathered is sufficiently precise to inform management decisions. Participants regularly report because the demands of the survey methodology have been matched to their time availability. Undertaking the RHIS protocol we describe involves three ~20 min surveys at each site. Participants enter data into an online data management system that can create reports for managers and participants within minutes of data being submitted. Since 2009, 211 participants have completed a total of more than 10,415 surveys at more than 625 different reefs. The two-way exchange of information between managers and participants increases the capacity to manage reefs adaptively, meets education and outreach objectives and can increase stewardship. The general approach used and the survey methodology are both sufficiently adaptable to be used in all reef regions.

Interictal psychosis following temporal lobe surgery: dentate gyrus pathology.

BACKGROUND: De novo interictal psychosis, albeit uncommon, can develop in patients following temporal lobe surgery for epilepsy. Pathological alterations of the dentate gyrus, including cytoarchitectural changes, immaturity and axonal reorganization that occur in epilepsy, may also underpin co-morbid psychiatric disorders. Our aim was to study candidate pathways that may be associated with the development of interictal psychosis post-operatively in patients with hippocampal sclerosis (HS). METHOD: A total of 11 patients with HS who developed interictal psychosis (HS-P) post-operatively were compared with a matched surgical HS group without psychosis (HS-NP). Resected tissues were investigated for the extent of granule cell dispersion, mossy fibre sprouting and calbindin expression in the granule cells. We quantified doublecortin, mini-chromosome maintenance protein 2 (MCM2) and reelin-expressing neuronal populations in the dentate gyrus as well as the distribution of cannabinoid type 1 receptor (CBR1). RESULTS: The patterns of neuronal loss and gliosis were similar in both groups. HS-P patients demonstrated less mossy fibre sprouting and granule cell dispersion (p < 0.01) and more frequent reduction in calbindin expression in granule cells. There were no group differences in the densities of immature MCM2, doublecortin and reelin-positive cells. CBR1 labelling was significantly lower in Cornu ammonis area CA4 relative to other subfields (p < 0.01); although reduced staining in all hippocampal regions was noted in HS-P compared with HS-NP patients, the differences were not statistically significant. CONCLUSIONS: The alterations in dentate gyrus pathology found in HS-P patients could indicate underlying differences in the cellular response to seizures. These mechanisms may predispose to the development of psychosis in epilepsy and warrant further investigation.

Strontium concentrations in corrosion products from residential drinking water distribution systems.

The United States Environmental Protection Agency (US EPA) will require some U.S. drinking water distribution systems (DWDS) to monitor nonradioactive strontium (Sr(2+)) in drinking water in 2013. Iron corrosion products from four DWDS were examined to assess the potential for Sr(2+) binding and release. Average Sr(2+) concentrations in the outermost layer of the corrosion products ranged from 3 to 54 mg kg(-1) and the Sr(2+) drinking water concentrations were all ≤0.3 mg L(-1). Micro-X-ray adsorption near edge structure spectroscopy and linear combination fitting determined that Sr(2+) was principally associated with CaCO3. Sr(2+) was also detected as a surface complex associated with α-FeOOH. Iron particulates deposited on a filter inside a home had an average Sr(2+) concentration of 40.3 mg kg(-1) and the associated drinking water at a tap was 210 µg L(-1). The data suggest that elevated Sr(2+) concentrations may be associated with iron corrosion products that, if disturbed, could increase Sr(2+) concentrations above the 0.3 µg L(-1) US EPA reporting threshold. Disassociation of very small particulates could result in drinking water Sr(2+) concentrations that exceed the US EPA health reference limit (4.20 mg kg(-1) body weight).

Measuring elastic constants of arbitrarily shaped samples using resonant ultrasound spectroscopy.

One of the most important undertakings for materials is the measurement of the elastic behavior. As derivatives of the free energy with respect to atomic displacements, the elastic properties are closely connected to the thermodynamic properties of the material. Elastic behavior is a sensitive probe of the lattice environment in which all solid state phenomena occur, particularly in the vicinity of a phase transition. A useful method for measuring elastic properties is resonant ultrasound spectroscopy (RUS). Some novel materials to which RUS might be applied are often fragile or chemically reactive so that they cannot be polished into the shapes required by conventional RUS; for such cases a finite element method may be used. In this paper a discussion and test of a finite element method for RUS with arbitrarily shaped samples is provided.

Speciation and distribution of vanadium in drinking water iron pipe corrosion by-products.

Vanadium (V) when ingested from drinking water in high concentrations (>15 µg L(-1)) is a potential health risk and is on track to becoming a regulated contaminant. High concentrations of V have been documented in lead corrosion by-products as Pb(5)(V(5+)O(4))(3)Cl (vanadinite) which, in natural deposits is associated with iron oxides/oxyhydroxides, phases common in iron pipe corrosion by-products. The extent of potential reservoirs of V in iron corrosion by-products, its speciation, and mechanism of inclusion however are unknown. The aim of this study is to assess these parameters in iron corrosion by-products, implementing synchrotron-based µ-XRF mapping and µ-XANES along with traditional physiochemical characterization. The morphologies, mineralogies, and chemistry of the samples studied are superficially similar to typical iron corrosion by-products. However, we found V present as discrete grains of Pb(5)(V(5+)O(4))(3)Cl likely embedded in the surface regions of the iron corrosion by-products. Concentrations of V observed in bulk XRF analysis ranged from 35 to 899 mg kg(-1). We calculate that even in pipes with iron corrosion by-products with low V concentration, 100 mg kg(-1), as little as 0.0027% of a 0.1-cm thick X 100-cm long section of that corrosion by-product needs to be disturbed to increase V concentrations in the drinking water at the tap to levels well above the 15 µg L(-1) notification level set by the State of California and could adversely impact human health. In addition, it is likely that large reservoirs of V are associated with iron corrosion by-products in unlined cast iron mains and service branches in numerous drinking water distribution systems.

Addition of adenoviral vector targeting of chemotherapy to the MUC-1/ecdCD40L VPPP vector prime protein boost vaccine prolongs survival of mice carrying growing subcutaneous deposits of Lewis lung cancer cells.

We studied the effect of adding chemotherapy or vector targeted chemotherapy to the administration of an Ad-sig-hMUC-1/ecdCD40L adenoviral vector prime-hMUC-1/ecdCD40L protein boost cancer vaccine (designated hMUC-1/ecdCD40L VPPP vaccine), which were administered to test mice 10 days following subcutaneous (s.c.) inoculation of 500,000 Lewis Lung Carcinoma cells, at a time when the average volume of the s.c. tumors was 50 cu mm. The survival of hMUC-1/ecdCD40L VPPP vaccine-treated mice was twice as long as untreated mice. Addition of vector-targeted chemotherapy (AdCMVCDIRESE1A/5FC) to the hMUC-1/ecdCD40L VPPP vaccine 10 days after tumor inoculation significantly (P=0.0062) prolonged the survival of the test mice over administration of the hMUC-1/ecdCD40L VPPP vaccine alone or the control mice (P<0.00001). Interestingly, the combination of the AdCMVCDIRESE1A/5FC vector-targeted chemotherapy to the hMUC-1/ecdCD40L VPPP vaccine decreased the levels of CD44(+)CD24⁻ cells in s.c. deposits of the human MUC-1-positive Lewis Lung Cancer cell line (LL2/LL1hMUC-1) by 20 fold. These results suggest that the addition of vector-targeted chemotherapy to an adenoviral-based cancer vaccine is a strategy that deserves further testing.

Annealing a magnetic cactus into phyllotaxis.

The appearance of mathematical regularities in the disposition of leaves on a stem, scales on a pine-cone, and spines on a cactus has puzzled scholars for millennia; similar so-called phyllotactic patterns are seen in self-organized growth, polypeptides, convection, magnetic flux lattices and ion beams. Levitov showed that a cylindrical lattice of repulsive particles can reproduce phyllotaxis under the (unproved) assumption that minimum of energy would be achieved by two-dimensional Bravais lattices. Here we provide experimental and numerical evidence that the Phyllotactic lattice is actually a ground state. When mechanically annealed, our experimental "magnetic cactus" precisely reproduces botanical phyllotaxis, along with domain boundaries (called transitions in Botany) between different phyllotactic patterns. We employ a structural genetic algorithm to explore the more general axially unconstrained case, which reveals multijugate (multiple spirals) as well as monojugate (single-spiral) phyllotaxis.