RESUMO
The demand for rare earth elements (REEs) is growing and as a result, environmental exposure is a concern. The objective of this research was to evaluate the acute toxicity of Tm to Hyalella azteca and to understand the potential for toxicity modification by dissolved organic matter (DOM) and the cations Ca2+, Mg2+ and Na+. Standard methods were followed for 96 h static exposures in a medium with a hardness of 60 mg CaCO3/L, pH of 7.3 at 23 °C. H. azteca neonates (2-9 d of age) were used and in unmodified media the LC50 concentration was 3.4 µM [95% CI 2.9-3.9 µM; 573 µg/L (482-663)] based on measured dissolved concentrations at the end of the test. Tests done with different concentrations of Ca (0.25, 0.5 and 1.5 mM) did not show consistent trends and there was no clear evidence of a protective effect from Ca. Variations in Na (0.26, 0.5 and 1.6 mM) resulted in no significant changes in toxicity. Similarly, Mg (0.07, 0.14 and 0.4 mM) did not result in significant changes in LC50 values, except for a reduction in toxicity for measured total Tm at the lowest Mg concentration. Our results indicate that Tm toxicity is not influenced by cationic competition (Ca, Na and Mg). Dissolved organic matter (sourced from Luther Marsh ON) offered significant protection against Tm toxicity. Addition of 9 mg DOC/L resulted in significantly increased LC50 values. This study contributes toward understanding the toxicity of Tm and the importance of considering dissolved organic matter in estimating the potential for environmental risk of REEs.
Assuntos
Anfípodes , Poluentes Químicos da Água , Animais , Cátions , Humanos , Recém-Nascido , Túlio , Testes de Toxicidade Aguda , Poluentes Químicos da Água/toxicidadeRESUMO
Dissolved organic carbon (DOC) is known to ameliorate the toxicity of the trace metal nickel (Ni) to aquatic animals. In theory, this effect is mediated by the capacity of DOC to bind Ni, rendering it less bioavailable, with the resulting reduction in accumulation limiting toxicological effects. However, there is a lack of experimental data examining Ni accumulation in marine settings with natural sources of DOC. In the current study, radiolabelled Ni was used to examine the time- and concentration-dependence of Ni accumulation, using naturally sourced DOC, on developing larvae of the sea urchin Strongylocentrotus purpuratus. Contrary to prediction, the two tested natural DOC samples (collected from the eastern United States, DOC 2 (Seaview park, Rhode Island (SVP)) and DOC 7 (Aubudon Coastal Center, Connecticut)) which had previously been shown to protect against Ni toxicity, did not limit accumulation. The control (artificial seawater with no added DOC), and the DOC 2 sample could mostly be described as having saturable Ni uptake, whereas Ni uptake in the presence of DOC 7 was mostly linear. These data provide evidence that DOC modifies the bioavailability of Ni, through either indirect effects (e.g. membrane permeability) or by the absorption of DOC-Ni complexes. There was some evidence for regulation of Ni accumulation in later-stage embryos (96-h) where the bioconcentration factor for Ni declined with increasing Ni exposure concentration. These data have implications for predictive modelling approaches that rely on known relationships between Ni speciation, bioavailability and bioreactivity, by suggesting that these relationships may not hold for natural marine DOC samples in the developing sea urchin model system.
Assuntos
Matéria Orgânica Dissolvida/farmacologia , Níquel/farmacocinética , Strongylocentrotus purpuratus/efeitos dos fármacos , Animais , Larva , Strongylocentrotus purpuratus/crescimento & desenvolvimento , Strongylocentrotus purpuratus/metabolismo , Poluentes Químicos da Água/farmacologiaRESUMO
The primary goal of the present study is to provide a broad view of best practices for evaluating bioavailability models for metals for use in the protection of aquatic life. We describe the state of the science regarding 1) the evaluation and selection of ecotoxicity data, 2) the selection of bioavailability models for use in normalization, and 3) subsequent application of bioavailability models. Although many examples of normalization steps exist worldwide, a scheme is proposed to evaluate and select a model that takes account of its representativeness (water chemistry and taxonomic coverage of the ecotoxicity data set) and validation performance. Important considerations for a suitable model are the quantity of inputs needed, accuracy, and ease of use, all of which are needed to set protective values for aquatic life and to use these values to evaluate potential risks to organisms in receiving waters. Although the end results of different model application approaches may be broadly similar, the differences in these application frameworks ultimately come down to a series of trade-offs between who needs to collect the data and use the bioavailability model, the different requirements of spatial scales involved (e.g., regional vs site-specific values), and model predictiveness and protectiveness. Ultimately, understanding the limits and consequences of these trade-offs allows for selection of the most appropriate model and application framework to best provide the intended levels of aquatic life protection. Environ Toxicol Chem 2019;39:118-130. © 2019 SETAC.
Assuntos
Organismos Aquáticos/metabolismo , Água Doce/química , Metais , Modelos Biológicos , Poluentes Químicos da Água , Animais , Organismos Aquáticos/efeitos dos fármacos , Disponibilidade Biológica , Metais/metabolismo , Metais/toxicidade , Medição de Risco , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/toxicidadeRESUMO
Cadmium is an endocrine disruptor and inhibits corticosteroid production, but the mechanisms are far from clear. We tested the hypothesis that sublethal exposure to environmentally realistic levels of cadmium impairs cortisol production by disrupting the melanocortin 2 receptor (MC2R) signaling in rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of cadmium (0.75 or 2.0 µg/L) in a flow-through system for 7 d and subjected to an acute secondary stressor to evoke a cortisol response. Cadmium exposure for 7 d did not affect plasma cortisol concentrations, but head kidney mc2r mRNA levels were higher than in control fish. The cortisol stress performance to a secondary-stressor was attenuated in the cadmium groups, and this corresponded with transient reduction in transcript abundance of mc2r and the gene encoding its accessory protein MRAP1 but not MRAP2 in the head kidney. Furthermore, in vivo cadmium exposure attenuated the adrenocorticotropic hormone (ACTH)-, but not 8-br-cAMP-stimulated cortisol production in head kidney slices ex vivo. This corresponded with reduced transcript abundance of mc2r and mrap1, but not mrap2 in these tissue slices. Also, reporter assays with CHO cells transiently transfected with rainbow trout mc2r and zebrafish mrap1 revealed a dose-independent inhibition in ACTH-stimulated luciferase activity by cadmium. Collectively, waterborne exposure to environmentally realistic concentration of cadmium compromises the stressor-induced cortisol response, and a mode of action involves the disruption of MC2R signaling in rainbow trout.
Assuntos
Cádmio/toxicidade , Oncorhynchus mykiss/metabolismo , Receptor Tipo 2 de Melanocortina/metabolismo , Transdução de Sinais/efeitos dos fármacos , Animais , Células CHO , Cricetinae , Cricetulus , Rim Cefálico/efeitos dos fármacos , Rim Cefálico/metabolismo , Hidrocortisona/sangue , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteínas Modificadoras da Atividade de Receptores/metabolismo , Poluentes Químicos da Água/toxicidadeRESUMO
Nickel (Ni) emissions resulting from production and transportation raise concerns about the impact of Ni exposure to marine ecosystems. Ni bioavailability models are established for FW systems, but the influence of chemical parameters (e.g. dissolved organic carbon (DOC)) on Ni toxicity within marine systems is less well understood. To examine the effects of DOC concentration and composition on Ni toxicity, acute toxicity tests were conducted on early life-stages of blue mussels (Mytilus edulis) and sea urchin embryos (Strongylocentrotus purpuratus) in full strength sea water (32 ppt). Nine different field collected samples of water with varying concentration (up to 4.5â¯mgâ¯C/L) and composition of DOC were collected from the east coast of the United States. Organic matter compositional analysis included molecular fluorescence and absorbance spectroscopy. The different DOC sources had different protective effects against embryo toxicity. The control (no DOC) Ni 48â¯h-EC50 for Mytilus embryos was 133⯵g/L (95% confidence interval (C.I.) of 123-144⯵g/L), while Strongylocentrotus embryos displayed control 96-h EC50 values of 207⯵g/L (167-247⯵g/L). The most significantly protective sample had high humic acid concentrations (as determined from fluorescence spectroscopy), which yielded an EC50 of 195⯵g/L (169-222⯵g/L) for Mytilus, and an EC50 of 394⯵g/L (369-419⯵g/L) for S. purpuratus. Among all samples, protection was related to both DOC quantity and quality, with fluorescence-resolved humic and fulvic acid concentrations showing the strongest correlations with protection for both species. These data suggest that DOC is protective against Ni toxicity in M. edulis and S. purpuratus, and that accounting for a DOC quality factor will improve predictive toxicity models such as the biotic ligand model.
Assuntos
Benzopiranos/análise , Carbono/análise , Substâncias Húmicas/análise , Mytilus edulis/efeitos dos fármacos , Níquel/toxicidade , Strongylocentrotus purpuratus/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Feminino , Masculino , Água do Mar/química , Testes de Toxicidade AgudaRESUMO
Speciation of copper in marine systems strongly influences the ability of copper to cause toxicity. Natural organic matter (NOM) contains many binding sites which provides a protective effect on copper toxicity. The purpose of this study was to characterize copper binding with NOM using fluorescence quenching techniques. Fluorescence quenching of NOM with copper was performed on nine sea water samples. The resulting stability constants and binding capacities were consistent with literature values of marine NOM, showing strong binding with [Formula: see text] values from 7.64 to 10.2 and binding capacities ranging from 15 to 3110 nmol mg [Formula: see text] Free copper concentrations estimated at total dissolved copper concentrations corresponding to previously published rotifer effect concentrations, in the same nine samples, were statistically the same as the range of free copper calculated for the effect concentration in NOM-free artificial seawater. These data confirms the applicability of fluorescence spectroscopy techniques for NOM and copper speciation characterization in sea water and demonstrates that such measured speciation is consistent with the chemical principles underlying the biotic ligand model approach for bioavailability-based metals risk assessment.
Assuntos
Cobre/química , Modelos Químicos , Poluentes Químicos da Água/química , Animais , Cobre/análise , Fluorescência , Ligantes , Metais/química , Rotíferos , Água do Mar/química , Poluentes Químicos da Água/análiseRESUMO
The emergence of omics approaches in environmental research has enhanced our understanding of the mechanisms underlying toxicity; however, extrapolation from molecular effects to whole-organism and population level outcomes remains a considerable challenge. Using environmentally relevant, sublethal, concentrations of two metals (Cu and Ni), both singly and in binary mixtures, we integrated data from traditional chronic, partial life-cycle toxicity testing and metabolomics to generate a statistical model that was predictive of reproductive impairment in a Daphnia pulex-pulicaria hybrid that was isolated from an historically metal-stressed lake. Furthermore, we determined that the metabolic profiles of organisms exposed in a separate acute assay were also predictive of impaired reproduction following metal exposure. Thus we were able to directly associate molecular profiles to a key population response - reproduction, a key step towards improving environmental risk assessment and management.
Assuntos
Cobre/toxicidade , Daphnia/metabolismo , Ecotoxicologia/métodos , Metaboloma , Níquel/toxicidade , Reprodução/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Daphnia/efeitos dos fármacos , Daphnia/fisiologia , Ecotoxicologia/estatística & dados numéricos , Genética Populacional , Metabolômica , Modelos Estatísticos , Medição de Risco , Fatores de TempoRESUMO
This study investigates the effect of chronic Cd exposure on the ability to perform repeat swim challenges in brown trout (Salmo trutta) and lake whitefish (Coregonus clupeaformis). Fish were exposed to waterborne Cd (18nM) in moderately hard water (120mgL(-1) CaCO3) for 30 days. This level of exposure has been shown to cause sublethal physiological disruption and acclimation responses but no impairment of sustained swimming capacity (Ucrit) in single swim challenges. Swim trials were done over the course of the exposure and each one consisted of an initial swim to 85% of the Ucrit of control fish, a 30min recovery period and finally a second swim challenge to determine Ucrit. Plasma and tissue samples were collected before and after each of the swim periods. As expected from previous studies, Cd exposure resulted in significant accumulation of Cd in gills, liver and kidney but not in white muscle. Exposure also induced a loss of plasma Ca followed by subsequent recovery (in lake whitefish but not brown trout) with few mortalities (100% survival for lake whitefish and 93% for brown trout). Both control and exposed fish swam to 85% of the single swim Ucrit and no differences in performance were seen. The Ucrit of unexposed controls in the second swim challenges were not different from the single swim Ucrit. However, second swim performance was significantly reduced in Cd exposed fish, particularly after a week of exposure where 31% and 38% reductions were observed for brown trout and lake whitefish respectively. Swimming to 85% Ucrit resulted in metabolic expenditure with little recovery after 30min. Few differences were observed between control and Cd exposed fish with the exception of a reduction in resting white muscle ATP stores of Cd exposed fish after 1 week of exposure. The results show that chronic sublethal Cd exposure results in an impairment of swimming ability in repeat swim challenges but this impairment is generally not related to metabolic processes in white muscle.
Assuntos
Cádmio/toxicidade , Metabolismo Energético/efeitos dos fármacos , Salmonidae/fisiologia , Natação , Truta/fisiologia , Anaerobiose/efeitos dos fármacos , Animais , Cádmio/metabolismo , Exposição Ambiental , Lagos/química , Distribuição Tecidual , Poluentes Químicos da Água/metabolismo , Poluentes Químicos da Água/toxicidadeRESUMO
Reductions in atmospheric emissions from the metal smelters in Sudbury, Canada, produced major improvements in acid and metal contamination of local lakes and indirectly increased dissolved organic carbon (DOC) concentrations. Metal toxicity, however, has remained a persistent problem for aquatic biota. Integrating high-throughput, nontargeted mass spectrometry metabolomics with conventional toxicological measures elucidated the mediating effects of dissolved organic matter (DOM) on the toxicity of Cu to Daphnia pulex-pulicaria, a hybrid isolated from these soft water lakes. Two generations of daphniids were exposed to Cu (0-20 µg/L) at increasing levels of natural DOM (0-4 mg DOC/L). Added DOM reduced Cu toxicity monotonically with median lethal concentration values increasing from 2.3 µg/L Cu without DOM to 22.7 µg/L Cu at 4 mg DOC/L. Reproductive output similarly benefited, increasing with DOM, yet falling with increases in Cu. Second generation reproduction was more impaired than the first generation. Dissolved organic matter had a greater influence than Cu on the metabolic status of the daphniids. Putative identification of metabolite peaks indicated that DOM elevation increased the metabolic energy status of the first generation animals, but this benefit was reduced in the second generation, although evidence of increased oxidative stress was detected. These results indicate that Sudbury's terrestrial ecosystems should be managed to increase aquatic DOM supply to enable daphniid colonists to both survive and foster stable populations.
Assuntos
Carbono/metabolismo , Cobre/toxicidade , Metabolômica , Animais , Canadá , Carbono/química , Cobre/metabolismo , Daphnia/efeitos dos fármacos , Daphnia/crescimento & desenvolvimento , Análise Discriminante , Análise dos Mínimos Quadrados , Dose Letal Mediana , Espectrometria de Massas , Reprodução/efeitos dos fármacosRESUMO
The toxicological understanding of rare earth elements (REEs) in the aquatic environment is very limited but of increasing concern. The objective of this research is to compare the toxicological effect of the REE dysprosium to the freshwater invertebrates Daphnia pulex and Hyalella azteca and in the more sensitive organism, understand the toxicity modifying influence of Ca, Na, Mg, pH and dissolved organic matter (DOM). Standard methods (Environment Canada) were followed for testing and culture in media of intermediate hardness (60mg CaCO3 mg/L) at pH 7.8 with Ca at 0.5, Na 0.5, Mg 0.125 (mM) and 23°C. Acute toxicity tests were done with <24h old neonates for 48h in the case of D. pulex and with 2-9 days old offspring for 96h tests with Hyalella. The potential protective effect of cationic competition was tested with Ca (0.5-2.0mM), Na (0.5-2.0mM) and Mg (0.125-0.5mM). The effect of pH (6.5-8.0) and Suwannee River DOM complexation (at dissolved organic carbon (DOC) concentrations of 9 and 13mg C/L) were evaluated. Dissolved Dy concentrations were lower than total (unfiltered) indicating precipitation, particularly at higher concentrations. Acute toxicity of Dy to H. azteca and D. pulex revealed Hyalella to be 1.4 times more sensitive than Daphnia. Additions of Ca and Na but not Mg provided significant protection against Dy toxicity to Hyalella. Similarly, low pH was associated with reduction in toxicity. Exposures which were pH buffered with and without MOPS were significantly different and indicated that MOPS enhanced Dy toxicity. DOM also mitigated Dy toxicity. Biotic ligand based parameters (LogK values) were calculated based on free ion relationships as determined by geochemical equilibrium modeling software (WHAM ver. 7.02). The logK value for Dy(3+) toxicity to Hyalella was 7.75 while the protective influence of Ca and Na were 3.95 and 4.10, respectively. This study contributes data towards the development of site specific water quality guidelines and criteria for Dy and possibly REEs in general and offers insight into the complex bio-geochemical nature of this element.
Assuntos
Anfípodes/efeitos dos fármacos , Daphnia/efeitos dos fármacos , Disprósio/toxicidade , Poluentes Químicos da Água/toxicidade , Anfípodes/fisiologia , Animais , Cálcio/química , Daphnia/fisiologia , Concentração de Íons de Hidrogênio , Dose Letal Mediana , Ligantes , Magnésio/química , Sódio/química , Temperatura , Testes de Toxicidade Aguda , Poluentes Químicos da Água/químicaRESUMO
Acute copper (Cu) toxicity tests (48-h LC50) using the euryhaline rotifer Brachionus plicatilis were performed to assess the effects of salinity (3, 16, 30 ppt) and dissolved organic carbon (DOC, â¼ 1.1, â¼ 3.1, â¼ 4.9, â¼ 13.6 mg C L(-1)) on Cu bioavailability. Total Cu was measured using anodic stripping voltammetry, and free Cu(2+) was measured using ion-selective electrodes. There was a protective effect of salinity observed in all but the highest DOC concentrations; at all other DOC concentrations the LC50 value was significantly higher at 30 ppt than at 3 ppt. At all salinities, DOC complexation significantly reduced Cu toxicity. At higher concentrations of DOC the protective effect increased, but the increase was less than expected from a linear extrapolation of the trend observed at lower concentrations, and the deviation from linearity was greatest at the highest salinity. Light-scattering data indicated that salt induced colloid formation of DOC could be occurring under these conditions, thereby decreasing the number of available reactive sites to complex Cu. When measurements of free Cu across DOC concentrations at each individual salinity were compared, values were very similar, even though the total Cu LC50 values and DOC concentrations varied considerably. Furthermore, measured free Cu values and predicted model values were comparable, highlighting the important link between the concentration of bioavailable free Cu and Cu toxicity.
Assuntos
Carbono/farmacologia , Cobre/toxicidade , Compostos Orgânicos/farmacologia , Rotíferos/efeitos dos fármacos , Salinidade , Testes de Toxicidade Aguda , Animais , Intervalos de Confiança , Ligantes , Modelos Teóricos , Rotíferos/metabolismoRESUMO
The physiological responses to waterborne cadmium exposure have been well documented; however, few studies have examined animal performances at low exposure concentrations of this metal. We tested the hypothesis that longer-term exposure to low levels of cadmium will compromise the steroidogenic and metabolic capacities, and reduce the cortisol response to a secondary stressor in fish. To test this, juvenile rainbow trout (Oncorhynchus mykiss) were exposed to 0 (control), 0.75 or 2.0 µg/L waterborne cadmium in a flow-through system and were sampled at 1, 7 and 28 d of exposure. There were only very slight disturbances in basal plasma cortisol, lactate or glucose levels in response to cadmium exposure over the 28 d period. Chronic cadmium exposure significantly affected key genes involved in corticosteroidogenesis, including melanocortin 2 receptor, steroidogenic acute regulatory protein and cytochrome P450 side chain cleavage enzyme. At 28 d, the high cadmium exposure group showed a significant drop in the glucocorticoid receptor and mineralocorticoid receptor protein expressions in the liver and brain, respectively. There were also perturbations in the metabolic capacities in the liver and gill of cadmium-exposed trout. Subjecting these fish to a secondary handling disturbance led to a significant attenuation of the stressor-induced plasma cortisol, glucose and lactate levels in the cadmium groups. Collectively, although trout appears to adjust to subchronic exposure to low levels of cadmium, it may be at the cost of impaired interrenal steroidogenic and tissue-specific metabolic capacities, leading to a compromised secondary stress performance in rainbow trout.
Assuntos
Cádmio/toxicidade , Exposição Ambiental , Hidrocortisona/metabolismo , Fígado/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Ativação Enzimática/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Brânquias/efeitos dos fármacos , Manobra Psicológica , Rim Cefálico/efeitos dos fármacos , Fígado/enzimologia , Oncorhynchus mykiss/fisiologia , Receptores de Glucocorticoides/genética , Receptores de Mineralocorticoides/genéticaRESUMO
Interest in the interactions between dietary constituents in the gut is increasing, but information remains sparse. In this study rainbow trout were fed non-enriched (186.7±19.0 µg Zn g(-1) (dw)), enriched (20% increase) and hyper-enriched Zn (200% increase) diet for 21 d followed by a single meal of Cd-spiked food (188.6±9.9 µg Cd g(-1) (dw)). Intestinal, hepatic and renal Zn burdens were measured on Days 7, 14 and 21 and Cd concentrations in the same tissue were measured 48 h-post Cd exposure. Oxidative stress was measured as lipid peroxidation in dissected tissues and intestinal mucus was quantified as sialic acid using the thiobarbituric acid assay. Rainbow trout maintained on the hyper-enriched Zn diet experienced significantly increased intestinal mucus secretion (p<0.01), were the only treatment group not to accumulate Cd in the intestine, and there was also no increase in intestinal oxidative damage. Conversely, fish fed the non-enriched and enriched Zn diets did not produce greater than basal levels of intestinal mucus and accumulated significantly greater concentrations of Cd in the intestine (p<0.01) leading to significant localised Cd-induced lipid peroxidation (p<0.01). High levels of mucus production correlated to lower incidences of lipid peroxidation (r(2)=0.54, p<0.05). These results demonstrate that mucus production stimulated by a high Zn diet have an inhibitory effect on Cd accumulation in the intestine and on Cd-induced lipid peroxidation. Mechanistically, it is likely that the elevated mucus production provides a barrier to Cd uptake. This study describes how one dietary constituent directly modifies the gut environment which indirectly influences the fate of another ingested cation.
Assuntos
Cádmio/metabolismo , Suplementos Nutricionais , Intestinos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Muco/metabolismo , Oncorhynchus mykiss/fisiologia , Poluentes Químicos da Água/metabolismo , Zinco/farmacologia , Animais , Cádmio/análise , Cádmio/toxicidade , Oncorhynchus mykiss/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Zinco/análise , Zinco/metabolismoRESUMO
Using a 30-year record of biological and water chemistry data collected from seven lakes near smelters in Sudbury (Ontario, Canada) we examined the link between reductions of Cu, Ni, and Zn concentrations and zooplankton species richness. The toxicity of the metal mixtures was assessed using an additive Toxic Unit (TU) approach. Four TU models were developed based on total metal concentrations (TM-TU); free ion concentrations (FI-TU); acute LC50s calculated from the Biotic Ligand Model (BLM-TU); and chronic LC50s (acute LC50s adjusted by metal-specific acute-to-chronic ratios, cBLM-TU). All models significantly correlated reductions in metal concentrations to increased zooplankton species richness over time (p < 0.01) with a rank based on r(2) values of cBLM-TU > BLM-TU = FI-TU > TM-TU. Lake-wise comparisons within each model showed that the BLM-TU and cBLM-TU models provided the best description of recovery across all seven lakes. These two models were used to calculate thresholds for chemical and biological recovery using data from reference lakes in the same region. A threshold value of TU = 1 derived from the cBLM-TU provided the most accurate description of recovery. Overall, BLM-based TU models that integrate site-specific water chemistry-derived estimates of toxicity offer a useful predictor of biological recovery.
Assuntos
Biodiversidade , Lagos/química , Metais Pesados/toxicidade , Modelos Biológicos , Poluentes Químicos da Água/toxicidade , Zooplâncton/efeitos dos fármacos , Animais , Recuperação e Remediação Ambiental/estatística & dados numéricos , Dose Letal Mediana , Metalurgia , Metais Pesados/análise , Ontário , Especificidade da Espécie , Poluentes Químicos da Água/análise , Zooplâncton/fisiologiaRESUMO
Copper-induced toxicity in aqueous systems depends on its speciation and bioavailability. Natural organic matter (NOM) and reduced sulphur species can complex copper, influencing speciation and decreasing bioavailability. NOM composition in estuaries can vary, depending on inputs of terrigenous, autochthonous, or wastewater source material. At a molecular level, variability in NOM quality potentially results in different extents of copper binding. The aims of this study were to measure acute copper EC(50) values in coastal marine and estuarine waters, and identify the relationships between total dissolved copper EC(50) values and measured water chemistry parameters proportional to NOM and reduced sulphur composition. This has implications on the development of marine-specific toxicity prediction models. NOM was characterised using dissolved organic carbon (DOC) concentration and fluorescence measurements, combined with spectral resolution techniques, to quantify humic-, fulvic-, tryptophan-, and tyrosine-like fractions. Reduced sulphur was measured by the chromium-reducible sulphide (CRS) technique. Acute copper toxicity tests were performed on samples expressing extreme DOC, fluorescent terrigenous, autochthonous, and CRS concentrations. The results show significant differences in NOM quality, independent of DOC concentration. CRS is variable among the samples; concentrations ranging from 4 to 40 nM. The toxicity results suggest DOC as a very good predictive measure of copper EC(50) in estuaries (r(2)=0.87) independent of NOM quality. Furthermore, for filtered samples, CRS exists at concentrations that would be saturated with copper at measured EC(50), suggesting that while CRS might bind Cu and decrease bioavailability, it does not control copper speciation at toxicologically relevant concentrations and therefore is not a good predictive measure of copper toxicity in filtered samples.
Assuntos
Cobre/química , Água do Mar/química , Enxofre/química , Poluentes Químicos da Água/química , Animais , Cobre/análise , Cobre/toxicidade , Monitoramento Ambiental , Mytilus/efeitos dos fármacos , Enxofre/análise , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
As part of a SETAC Pellston Workshop, we evaluated the potential use of metal tissue residues for predicting effects in aquatic organisms. This evaluation included consideration of different conceptual models and then development of several case studies on how tissue residues might be applied for metals, assessing the strengths and weaknesses of these different approaches. We further developed a new conceptual model in which metal tissue concentrations from metal-accumulating organisms (principally invertebrates) that are relatively insensitive to metal toxicity could be used as predictors of effects in metal-sensitive taxa that typically do not accumulate metals to a significant degree. Overall, we conclude that the use of tissue residue assessment for metals other than organometals has not led to the development of a generalized approach as in the case of organic substances. Species-specific and site-specific approaches have been developed for one or more metals (e.g., Ni). The use of gill tissue residues within the biotic ligand model is another successful application. Aquatic organisms contain a diverse array of homeostatic mechanisms that are both metal- and species-specific. As a result, use of whole-body measurements (and often specific organs) for metals does not lead to a defensible position regarding risk to the organism. Rather, we suggest that in the short term, with sufficient validation, species- and site-specific approaches for metals can be developed. In the longer term it may be possible to use metal-accumulating species to predict toxicity to metal-sensitive species with appropriate field validation.
Assuntos
Metais/toxicidade , Poluentes da Água/toxicidade , Animais , Organismos Aquáticos , Previsões , Invertebrados , Metais/farmacocinética , Modelos Teóricos , Compostos Organometálicos/farmacocinética , Compostos Organometálicos/toxicidade , Distribuição Tecidual , Poluentes da Água/farmacocinéticaRESUMO
The present study examined the effect of dissolved organic carbon (DOC), both added and inherent, on Cu toxicity in glochidia, the larvae of freshwater mussels. Using incremental additions of natural DOC concentrate and reconstituted water, a series of acute copper toxicity tests were conducted. An increase in DOC from 0.7 to 4.4 mg C/L resulted in a fourfold increase (36-150 µg Cu/L) in the 24-h median effective concentration (EC50) and a significant linear relationship (r² = 0.98, p = 0.0008) between the DOC concentration and the Cu EC50 of Lampsilis siliquoidea glochidia. The ameliorating effect of added DOC on Cu toxicity was confirmed using a second mussel species, the endangered (in Canada) Lampsilis fasciola. The effect of inherent (i.e., not added) DOC on Cu toxicity was also assessed in eight natural waters (DOC 5-15 mg C/L). These experiments revealed a significant relationship between the EC50 and the concentration of inherent DOC (r² = 0.79, p = 0.0031) with EC50s ranging from 27 to 111 µg Cu/L. These laboratory tests have demonstrated that DOC provides glochidia with significant protection from acute Cu toxicity. The potential risk that Cu poses to mussel populations was assessed by comparing Cu and DOC concentrations from significant mussel habitats in Ontario to the EC50s. Although overall mean Cu concentration in the mussel's habitat was well below the acutely toxic level given the concentration of DOC, episodic Cu releases in low DOC waters may be a concern for the recovery of endangered freshwater mussels. The results are examined in the context of current Cu water quality regulations including the U.S. Environmental Protection Agency's (U.S. EPA) biotic ligand model.
Assuntos
Bivalves/efeitos dos fármacos , Cobre/toxicidade , Compostos Orgânicos/química , Poluentes Químicos da Água/química , Animais , Compostos Orgânicos/análise , Compostos Orgânicos/farmacologia , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/farmacologiaRESUMO
The goal of this study was to develop a biotic ligand model (BLM) to predict the acute toxicity of cadmium to Daphnia pulex. Organisms were cultured in moderately soft water and standard 48h acute toxicity tests were used to determine EC50s in various water chemistries where the effects of Ca(2+), Na(+), Mg(2+), Cl(-), K(+), pH, and two sources of natural organic matter (Suwannee River and Nordic Reservoir) were evaluated. Overall, toxicity responses were consistent with the free-ion activity model and the principles inherent in the BLM. Increases in Ca(2+) resulted in higher EC50s, indicating that Cd(2+) competes with Ca(2+) for uptake at the biotic ligand. Similar cation competition effects were observed when Mg(2+) was varied but with a less pronounced protective effect relative to Ca(2+). Changes in Na(+) and K(+) concentrations had no significant effect on Cd toxicity. EC50 values did not change significantly when pH was adjusted over a range from 8.0 to 6.1. Additions of natural organic matter resulted in elevated dissolved organic carbon (DOC) concentrations that significantly reduced Cd bioavailability via complexation of Cd(2+). An existing biotic ligand model (HydroQual BLM ver 2.2.3) was tested for its ability to predict acute Cd toxicity to D. pulex. Once the BLM was adjusted for the relatively sensitivity of D. pulex the protective effects of Ca and DOC could be predicted reasonably well but other test chemistries did not match with measured EC50s. Binding constants derived from the test results (logK(CaBL) of 4.1, logK(MgBL) of 3.7, logK(HBL) of 6.1 and logK(CdBL) of 7.0) were used to develop a modified BLM for the effects of Cd on D. pulex that accounted for the moderating effect of Ca and Mg on acute toxicity but overestimated the protective effect of DOC.
Assuntos
Cádmio/toxicidade , Daphnia/efeitos dos fármacos , Modelos Biológicos , Poluentes Químicos da Água/toxicidade , Animais , Cálcio/farmacologia , Dose Letal MedianaRESUMO
The goal of this study was to develop a biotic ligand model (BLM) for the acute toxicity of zinc to the Daphnia pulex in soft water. In different tests Ca (as CaSO(4)), Na (as NaCl), Mg (as MgSO(4)), K (as KCl), pH (using the buffer 3-(N morpholino)-propanesulphonic acid (MOPS)) and dissolved organic carbon (DOC) were altered to determine possible effects on the 48h EC50 for Zn. Increases in waterborne Ca(2+) had a protective effect on Zn toxicity, suggesting that this ion competes with Zn and that they share a common site of biological uptake. Increased waterborne Mg(2+) also reduced Zn toxicity, but to a lesser degree compared with Ca(2+). No significant effects of other cations on EC50 for Zn were observed, indicating that the toxicity of Zn in D. pulex is not linked to Na(+) and K(+). Increasing DOC concentrations resulted in higher EC50 values for Zn due to the complexation of Zn by organic matter in solution and the resulting reduction of free Zn(2+) ion concentrations. Tests to characterize the effect of pH on Zn toxicity showed a small rise in EC50 values between pH 6.3 and 7.1 and no further change as pH was increased to 8.0. Two existing BLM implementations for acute Zn effects on D. magna were tested to determine their applicability to D. pulex in soft water. The existing models underestimated the protective effect of Ca(2+) and Mg(2+) but accurately predict the effects of DOC. A modified BLM, with revised equilibrium constants for competitive cation effects was developed. This study shows that the acute effects of Zn on D. pulex in soft water can be characterized and incorporated into a predictive BLM.