The Validity of Resting Metabolic Rate-Prediction Equations and Reliability of Measured RMR in Female Athletes.

The aim of the current study was to; 1) assess the test-retest reliability of an indirect calorimetry analyzer (Parvo Medics TrueOne), and 2) compare measured RMR with three RMR-predictive (pRMR) equations in female athletes. In part one, 12 recreationally-exercising women (mean ± SD; age 27.5 ± 12.3 y) performed two RMR assessments, on separate days, utilising the Parvo Medics TrueOne analyser. In part two, 25 recreationally-exercising women to sub-elite athletes (mean ± SD; age 30.1 ± 10.2 y) underwent an RMR assessment using the Parvo Medics TrueOne analyser, which was compared to three calculated pRMR equations (Harris-Benedict (H-B), Mifflin-St Jeor (M), World Health Organisation (WHO)). eTest-retest reliability for the TrueOne analyser was deemed acceptable (CV = 5.3%, ICC = 0.92). The validity of pRMR when compared to measured RMR showed low levels of agreement in all 3 predictive equations (M: CV = 21.4%, TEE = 269 kcal.day-1, r = 0.16, WHO: CV = 21.5%, TEE = 270 kcal.day-1, r = 0.13 H-B: CV = 21.6%, TEE = 270 kcal.day-1, r = 0.13). The Parvo Medics TrueOne analyser is a reliable tool for measuring RMR. Caution should be taken when using pRMR equations in female athletes as they do not take into account the likely differences in fat free mass in these populations.

The Effect of Medium Chain Triglycerides on Time to Nutritional Ketosis and Symptoms of Keto-Induction in Healthy Adults: A Randomised Controlled Clinical Trial.

Medium chain triglycerides (MCTs) are ketogenic and might reduce adverse effects of keto-induction and improve time to ketosis and the tolerability of very low carbohydrate diets. This study investigates whether MCT supplementation improves time to nutritional ketosis (NK), mood, and symptoms of keto-induction. We compared changes in beta-hydroxybutyrate (BOHB), blood glucose, symptoms of keto-induction, and mood disturbance, in 28 healthy adults prescribed a ketogenic diet, randomised to receive either 30 ml of MCT, or sunflower oil as a control, three times per day, for 20 days. The primary outcome measured was the achievement of NK (≥0.5 mmol·L-1 BOHB). Participants also completed a daily Profile of Mood States and keto-induction symptom questionnaire. MCT resulted in higher BOHB at all time points and faster time to NK, a result that failed to reach significance. Symptoms of keto-induction resulted from both diets, with a greater magnitude in the control group, except for abdominal pain, which occurred with greater frequency and severity in the MCT-supplemented diet. There was a possibly beneficial effect on symptoms by MCT, but the effect on mood was unclear. Based on these results, MCTs increase BOHB compared with LCT and reduce symptoms of keto-induction. It is unclear whether MCTs significantly improve mood or time to NK. The trial was registered by the Australia New Zealand Clinical Trial Registry ACTRN12616001099415.

The Effect of Nitrate Supplementation on Cycling Performance in the Heat in Well-Trained Cyclists.

PURPOSE: To determine the effect of [Formula: see text] consumption on measures of perception, thermoregulation, and cycling performance in hot conditions. METHODS: In a randomized, double-blind, crossover design, 8 well-trained cyclists (mean ± SD age 25 ± 8 y, [Formula: see text] peak 64 ± 5 mL · kg-1 · min-1) performed 2 separate trials in hot (35°C, 60% relative humidity) environments, having ingested either 140 mL [Formula: see text]-rich beetroot juice ∼8 mmol [Formula: see text] (NIT) or placebo (PLA) daily for 3 d with a 7-d washout period separating trials. Trials consisted of 2 × 10-min bouts at 40% and 60% peak power output (PPO) to determine physiological and perceptual responses to the heat, followed by a 4-km cycling time trial. RESULTS: Basal [nitrite] was substantially elevated in NIT (2.70 ± 0.98 µM) vs PLA (1.10 ± 0.61 µM), resulting in a most likely (ES = 1.58 ± 0.93) increase after 3 d. There was a very likely trivial increase in rectal temperature in NIT at 40% (PLA 37.4°C ± 0.2°C vs NIT 37.5°C ± 0.3°C, 0.1°C ± 0.2°C) and 60% (PLA 37.8°C ± 0.2°C vs NIT 37.9°C ± 0.3°C, 0.1°C ± 0.2°C) PPO. Cycling performance was similar between trials (PLA 336 ± 45 W vs NIT 337 ± 50 W, CV ± 95%CL; 0.2% ± 2.5%). Outcomes for heart rate and perceptual measures were unclear across the majority of time points. CONCLUSIONS: Three days of [Formula: see text] supplementation resulted in small increases in rectal temperature during low- to moderate-intensity exercise, but this did not appear to influence 4-km cycling time-trial performance in hot climates.

Dietary Nitrate Fails to Improve 1 and 4 km Cycling Performance in Highly Trained Cyclists.

We aimed to compare the effects of two different dosing durations of dietary nitrate (NO3-) supplementation on 1 and 4 km cycling time-trial performance in highly trained cyclists. In a double-blind crossover-design, nine highly trained cyclists ingested 140ml of NO3- -rich beetroot juice containing ~8.0mmol [NO3-], or placebo, for seven days. Participants completed a range of laboratory-based trials to quantify physiological and perceptual responses and cycling performance: time-trials on day 3 and 6 (4km) and on day 4 and 7 (1km) of the supplementation period. Relative to placebo, effects following 3- and 4-days of NO3- supplementation were unclear for 4 (-0.8; 95% CL, ± 2.8%, p = .54) and likely harmful for 1km (-1.9; ± 2.5% CL, p = .17) time-trial mean power. Effects following 6- and 7-days of NO3- supplementation resulted in unclear effects for 4 (0.1; ± 2.2% CL, p = .93) and 1km (-0.9; ± 2.6%CL, p = .51) time-trial mean power. Relative to placebo, effects for 40, 50, and 60% peak power output were unclear for economy at days 3 and 6 of NO3- supplementation (p > .05). Dietary NO3- supplementation appears to be detrimental to 1km time-trial performance in highly trained cyclists after 4-days. While, extending NO3- dosing to ≥ 6-days reduced the magnitude of harm in both distances, overall performance in short duration cycling time-trials did not improve relative to placebo.

The Effect of Dietary Nitrate Supplementation on Physiology and Performance in Trained Cyclists.

PURPOSE: To determine the effect of dietary nitrate (NO3-) supplementation on physiology and performance in well-trained cyclists after 6-8 d of NO3- supplementation. METHODS: Eight competitive male cyclists (mean ± SD age 26 ± 8 y, body mass 76.7 ± 6.9 kg, VO2peak 63 ± 4 mL · kg-1 · min-1) participated in a double-blind, placebo-controlled, crossover-design study in which participants ingested 70 mL of beetroot juice containing ~4 mmol NO3- (NIT) or a NO3--depleted placebo (PLA), each for 8 d. Replicating pretreatment measures, participants undertook an incremental ramp assessment to determine VO2peak and first (VT1) and second (VT2) ventilatory thresholds on d 6 (NIT6 and PLA6), moderate-intensity cycling economy on d 7 (NIT7 and PLA7), and a 4-km time trial (TT) on d 8 (NIT8 and PLA8). RESULTS: Relative to PLA, 6 d of NIT supplementation produced unclear effects for VO2peak (mean ± 95% confidence limit: 1.8% ± 5.5%) and VT1 (3.7% ± 12.3%) and trivial effects for both VT2 (-1.0% ± 3.0%) and exercise economy on d 7 (-1.0% ± 1.6%). However, effects for TT performance time (-0.7% ± 0.9%) and power (2.4% ± 2.5%) on d 8 were likely beneficial. CONCLUSIONS: Despite mostly unclear outcomes for standard physiological determinants of performance, 8 d of NO3- supplementation resulted in likely beneficial improvements to 4-km TT performance in well-trained male endurance cyclists.