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Aim: Natural medicine used as an alternative and/or complementary treatment to counteract diseases is of great importance in public health. Therefore, the purpose of the present study was to assess the in vitro antifungal activity of Morinda citrifolia methanolic extract of peel, pulp, and seed against Candida albicans. Materials and Methods: The present study was experimental in vitro and cross-sectional. Eight replicates were prepared in Sabouraud dextrose agar with five wells each, where 0.12% chlorhexidine, distilled water, and methanolic extract of seed, peel, and pulp of Morinda citrifolia fruit were placed at concentrations of 10,690, 8,270, and 6,430 mg/mL, respectively, to evaluate sensitivity according to Duraffourd's scale. In addition, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by dilution and agar seeding method. Statistical analysis was performed by analysis of variance (ANOVA) and Tukey's post hoc test, considering a significance level of P < 0.05. Results: The inhibition halos of Morinda citrifolia methanolic extract of seed, peel, and pulp against Candida albicans measured on average 15.94, 11.94, and 11.56 mm, respectively. The MIC of seed, peel, and pulp extract were 1366.25, 2067.5, and 1607.5 mg/mL respectively, whereas the MFC for seed, peel, and pulp extract were 2672.50, 2067.5, and 3215 mg/mL, respectively. Moreover, seed extract presented significantly higher antifungal activity than peel and pulp (P < 0.001). Conclusions: Morinda citrifolia methanolic extract of peel, pulp, and seed showed fungistatic and fungicidal effect against Candida albicans, being this very sensitive to seed extract with a MIC of 1366.25 mg/mL and a MFC of 2672.5 mg/mL, which allows recommending the development of effective pharmacological formulations for the control of candidiasis.
RESUMO
BACKGROUND: Metalloproteinases are proteolytic enzymes that degrade dentin and periodontal collagen; therefore, it is of special interest that dental students know its mechanism of action and how its effects can be inhibited. Therefore, the aim of this research was to evaluate the level of knowledge about metalloproteinases in dental students close to graduate from three universities in Peruvian capital city. MATERIALS AND METHODS: In this analytical, observational, cross-sectional and prospective study, 223 dental students close to graduate in three Peruvian universities were evaluated from September to November 2020. A questionnaire of 20 closed questions with three answers was validated and used to measure the level of general and dental knowledge about metalloproteinases. A logit model was used to evaluate the influence of the following variables: "gender" (X1), "type of university" (X2), "marital status" (X3) and "age group" (X4), in the knowledge levels of the students, considering a p-value <.05. RESULTS: Of the 223 dentistry students, it was obtained that the level of knowledge about metalloproteinases was predominantly low with 82.1%, (95% confidence interval (CI): 77.1%-87.1%) of the total. According to multivariate logistic regression analysis, "type of university" was the only variable that proved to have a significant influence (p = .022) on the level of knowledge about metalloproteinases with an odds ratio of (OR = 0.44; CI: 0.21-0.89), whilst the other variables "age group" (p > .05), "gender" (p = .058) and "marital status" (p = .114) were not considered influential factors. CONCLUSION: The majority of final year dental students in three Peruvian universities presented a low level of overall knowledge about metalloproteinases. In addition, private university students were 56% less likely to pass the subject knowledge test. On contrary, gender, age group and marital status did not significantly influence the level of knowledge of the students.