RESUMO
Data characterizing the kinetics of the contraction of trabeculae and the intertrabelcular tissue have been obtained. It has been concluded that trabeculae can act as conductors/transmitters of contraction across the myocardium and play a leading role in this process, while the functional role of the intertrabelcular tissue is secondary and more passive, and consists in the optimization and support of the contractile process.
Assuntos
Coração/fisiologia , Sódio/farmacologia , Animais , Cátions Monovalentes , Temperatura Baixa , Meios de Cultura , Átrios do Coração , Contração Miocárdica , Rana temporariaRESUMO
AIM: Optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [CMV]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (PCR) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation. MATERIALS AND METHODS: Sixty-three women with tubal-peritoneal infertility were included in the study. Immunoenzyme assay, PCR, and modified Griess method were used. RESULTS: In cervical canal herpesvirus infection was detected together with other agents in 30.1% of cases. In upper parts of reproductive system monoinfection with herpesvirus was detected in 74.63% of cases (herpes virus types 1 and 2--in 46.03%, CMV--in 28.6% of women). Increased levels of stable end metabolites of nitric oxide and cytokines were noted in sera of tested women. CONCLUSION: Inflammatory changes in reproductive organs in women with tubal-peritoneal infertility are determined by chronic herpesvirus infection. This leads to Th1 polarization of immune response and developing of immune-mediated inflammation.
Assuntos
DNA Viral/análise , Herpes Genital/diagnóstico , Herpesvirus Humano 1/isolamento & purificação , Herpesvirus Humano 2/isolamento & purificação , Infertilidade Feminina/virologia , Adulto , Colo do Útero/virologia , Doença Crônica , Citocinas/sangue , Citomegalovirus/genética , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/complicações , Infecções por Citomegalovirus/diagnóstico , Tubas Uterinas/virologia , Feminino , Herpes Genital/complicações , Herpes Genital/virologia , Herpesvirus Humano 1/genética , Herpesvirus Humano 2/genética , Humanos , Infertilidade Feminina/sangue , Óxido Nítrico/sangue , Óxido Nítrico/metabolismoRESUMO
AIM: To study the composition of microflora of lower and upper parts of reproductive system in women with tubal-peritoneal sterility. MATERIALS AND METHODS: One hundred and forty-five women with tubal-peritoneal sterilitywere examined. Laparoscopy with biopsy of ovaries and endometrium with evaluation of specimens by histology and PCR was performed in all patients. Cervical material, endometrial and ovarian biopsy specimens, and peritoneal fluid were tested by PCR on the presence of genetic material of sexually-transmitted microorganisms. Cervical material was tested for human papillomaviruses (types 16 and 18). RESULTS: Bacteria were isolated from cervical canal specimens in 40% of women. Chlamydia, Ureaplasma, herpes virus type 1 and 2, cytomegalovirus were detected in specimens of cervical canal, endometrium, ovaries and peritoneal fluid in 80%, 38,6%, 34,3%, and 38,46% of women, respectively. CONCLUSION: In cases of tubal-peritoneal sterility female reproductive system is infected with different pathogenic microorganisms. Growth of microorganisms was detected in 40% of cervical mucosa samples. Intracellular pathogens persist in upper and lower parts of female reproductive system. It was noted that Herpes simplex infection predominated in ovaries.
Assuntos
Infertilidade Feminina/microbiologia , Adulto , Líquido Ascítico/microbiologia , Biópsia , Colo do Útero/microbiologia , Chlamydia/isolamento & purificação , Endométrio/microbiologia , Endométrio/patologia , Feminino , Humanos , Infertilidade Feminina/patologia , Mucosa/microbiologia , Ovário/microbiologia , Ovário/patologia , Simplexvirus/isolamento & purificação , Ureaplasma/isolamento & purificação , Cervicite Uterina/microbiologiaRESUMO
Blood erythropoietin level was investigated in females of reproductive age, who had normal peripheral red blood findings. It was determined that blood erythropoietin level was 1.5-2 times higher (20.04 +/- 2.10; 28.57 +/- 5.87 mU/ml) in examined females with increased mitotic activity and hyperplasia signs of cervical squamous epithelia compared with female having normal cervix (12.73 +/- 0.96 mU/ml). In females with chronic cervicitis, blood erythropoietin level was decreased (8.87 +/- 1.72 mU/ml).
Assuntos
Eritropoetina/metabolismo , Reprodução/fisiologia , Adolescente , Adulto , Colo do Útero/metabolismo , Colo do Útero/patologia , Hiperplasia Endometrial/metabolismo , Hiperplasia Endometrial/patologia , Epitélio/metabolismo , Epitélio/patologia , Eritropoetina/sangue , Feminino , Humanos , Cervicite Uterina/metabolismo , Cervicite Uterina/patologiaRESUMO
The role of immune disturbances on the systemic and local levels is evaluated and the immunological mechanisms of tuboperitoneal sterility as the basis for the development of the methods of immunocorrection is grounded.
Assuntos
Infertilidade Feminina/imunologia , Linfócitos/imunologia , Antígenos CD/análise , Líquido Ascítico/imunologia , Proteínas do Sistema Complemento/análise , Tubas Uterinas/imunologia , Feminino , Humanos , Infertilidade Feminina/sangue , Interleucina-2/análise , Contagem de Linfócitos , Receptores de Interleucina-2/análise , Salpingite/imunologiaRESUMO
A total of 145 women with chronic inflammatory diseases of uterus and appendages (IDUA) were examined. Bacterioscopy of smears and culture of vaginal, cervical canal and uterine cavity contents were performed in all patients. Direct immunofluorescence and PCR were used for detection of chlamydiae in scrapes and smears and in biopsy specimens taken from endometrium. The number of T lymphocytes and their subpopulations, B lymphocytes as well as the level of plasmatic cells synthesizing IgA, IgM and IgG and secretory IgA (sIgA), immune complexes and C3 component of the complement were determined in biopsy specimens of the endometrium. Pathomorphological examination revealed structural changes characteristic of the immunocomplex disease (secondary deficiency of sIgA, pronounced suppression of IgA production by plasmocytes along with an essential increase in the number of IgG synthesizing cells and immune complexes fixed to the venule endothelium) were detected. Lectin-histochemical study revealed considerable changes in secretory activity of endometrial epitheliocytes manifested by severe suppression of bactericidal activity of the uterine mucus. The detected disturbances of local protective reactions in the endometrium of women IDUA gave grounds for including immunomodulators into the traditional treatment scheme. The latter made it possible to increase essentially the therapeutic effect.
Assuntos
Endometrite/imunologia , Doença Inflamatória Pélvica/imunologia , Adjuvantes Imunológicos/uso terapêutico , Técnicas Bacteriológicas , Doença Crônica , Endometrite/microbiologia , Endometrite/patologia , Feminino , Humanos , Doença Inflamatória Pélvica/microbiologia , Doença Inflamatória Pélvica/patologiaRESUMO
The etiological structure of the inflammatory diseases of genitals at different levels of the reproductive tract of women with tuboperitoneal sterility was studied. The study revealed the prevalence of cocci in the cervical canal, anaerobes in the endometrium and chlamydiae in the abdominal cavity (peritoneal fluid, the ovarian capsule, fimbriated ends), as well as the presence of mixed infection in 27% of the patients under study. The specific features of immunity in women with tuboperitoneal sterility were established, which determined the main trends of the subsequent pathogenetically oriented immunocorrection.
Assuntos
Infertilidade Feminina/etiologia , Infertilidade Feminina/imunologia , Peritonite/complicações , Salpingite/complicações , Adulto , Colo do Útero/microbiologia , Feminino , Humanos , Infertilidade Feminina/microbiologia , Cavidade Peritoneal/microbiologia , Peritonite/microbiologia , Salpingite/microbiologia , Útero/microbiologiaRESUMO
The population and subpopulation composition of peripheral blood lymphocytes of 33 women was studied in the dynamics of non-complicated pregnancy by the method of flow cytofluorometry with the use of equipment and reagents supplied by Becton Dickinson, Inc. This study revealed that the non-complicated course of pregnancy was characterized by the adequate reaction of the mother's immune system ensuring the physiological development of the fetus during pregnancy and timely labor.
Assuntos
Linfócitos/citologia , Gravidez/imunologia , Adulto , Antígenos CD19/análise , Complexo CD3/análise , Linfócitos T CD4-Positivos/citologia , Antígeno CD56/análise , Linfócitos T CD8-Positivos/citologia , Feminino , Citometria de Fluxo , Humanos , Contagem de Linfócitos , Subpopulações de Linfócitos/citologia , Receptores de IgG/análiseRESUMO
Local immune reactions in 107 women with Chlamydia-associated chronic endometritis and salpingo-oophoritis were studied on endometrial biopsy specimens. Of these women, group 1 consisted 45 patients with Chlamydia-associated chronic endometritis and salpingo-oophoritis and group 2 consisted of 62 such patients having, in addition, bacterial vaginosis and candidiasis. Among associated bacteria, epidermal staphylococci, Escherichia coli and their combinations prevailed. Pathomorphologically, in the biopsy specimens taken from the patients of group 1 a decrease in the activity of mononuclear phagocytes, secondary SIgA deficiency with the level of IgA-producing plasmocytes relatively unchanged, the moderate intensity of the synthesis of IgG and a shift in the ratio of T-helpers/T-suppressors towards the latter were observed. In the biopsy specimens of the uterine mucosa obtained from group 2 a decrease in the phagocytic activity of neutrophil granulocytes and macrophages, the pronounced suppression of IgA production by IgA plasmocytes with a sharp decrease in the amount of SIgA and an essential increase in the number of IgG-producing cells were noted. Disturbances of local immunity, observed in the presence of associated Chlamydia infection, gives grounds for the inclusion of immunomodulating preparations into the complex of therapeutic measures.
Assuntos
Infecções por Chlamydia/imunologia , Doença Inflamatória Pélvica/imunologia , Adulto , Formação de Anticorpos , Biópsia , Chlamydia/isolamento & purificação , Infecções por Chlamydia/microbiologia , Infecções por Chlamydia/patologia , Doença Crônica , Endométrio/patologia , Feminino , Humanos , Imunidade Celular , Doença Inflamatória Pélvica/microbiologia , Doença Inflamatória Pélvica/patologia , Esfregaço VaginalRESUMO
The character of microflora, directly causing inflammatory process in tuboperitoneal infertility, was studied. The study revealed the prevalence of microbial associations, Chlamydia infection was detected in half of the women, and in 17% of the patients this infection was for the first time detected in the upper section of their reproductive apparatus, which was indicative of the role of Chlamydia infection in the occlusion of the uterine tubes. Opportunistic microflora persisting in persons with defective antimicrobial protection was found. The study of local protective factors in peritoneal fluid revealed the absence of T-cell activity, which was manifested by a low level of sIL-2R and the absence of a significant rise in the level of IL-2 in peritoneal fluid in comparison with that in healthy women. In addition, a decrease in the total complement activity and in the activity of component C3 of the complement was established.
Assuntos
Líquido Ascítico/etiologia , Bactérias/isolamento & purificação , Candida/isolamento & purificação , Citomegalovirus/isolamento & purificação , Herpesvirus Humano 2/isolamento & purificação , Infertilidade Feminina/etiologia , Adulto , Formação de Anticorpos , Líquido Ascítico/imunologia , Líquido Ascítico/microbiologia , Líquido Ascítico/virologia , Colo do Útero/microbiologia , Colo do Útero/virologia , Doença Crônica , Feminino , Humanos , Infertilidade Feminina/imunologia , Infertilidade Feminina/microbiologia , Infertilidade Feminina/virologia , Útero/microbiologia , Útero/virologiaRESUMO
The correlation between the characteristics of K(+)-transport across the mitochondrial and bilayer lipid membranes formed form mitochondrial lipids has been demonstrated. It has been shown that different modes of K(+)-efflux activation in mitochondria result in the appearance of K(+)-transporting phospholipid forms. The experiments described in this work suggest that current fluctuations similar to those observed for biological channels can be registered in unmodified bilayer lipid membranes containing no protein components.
Assuntos
Membranas Intracelulares/metabolismo , Bicamadas Lipídicas/metabolismo , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Fosfolipídeos/metabolismo , Canais de Potássio/metabolismo , Potássio/metabolismo , Animais , Transporte Biológico , Condutividade Elétrica , Membranas Intracelulares/fisiologia , Membranas Artificiais , Permeabilidade , Ratos , Ratos WistarRESUMO
Using the procedure of lysis by ammonium chloride followed by differentiation centrifuging, the fraction of cells containing polymorphonuclear leucocytes was isolated from the whole rat blood to study the conditions of optimal production of oxygen forms was determined from the chemiluminescent cell response in the presence of luminol. It was shown that chemiluminescent cell response depends on the temperature, pH and osmolarity of the medium as well as on the presence of various concentrations of Ca2+ in the incubation medium. The maximal chemiluminescent response of polymorphonuclear leucocytes was observed at 35-37 degrees C, pH 7,6, osmolarity of 0,15 and Ca2+ concentration of 5-10 mM.
Assuntos
Neutrófilos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Animais , Cálcio/metabolismo , Células Cultivadas , Concentração de Íons de Hidrogênio , Medições Luminescentes , Concentração Osmolar , Ratos , Ratos Wistar , TemperaturaRESUMO
Immunologic homeostasis was studied in 272 women suffering from sterility of a tuboperitoneal and mixed origin. The detected drastic disorders of immunologic homeostasis were characterized by predominant disorders of primary immunity mechanisms (leukopenia, monocytopenia, lymphopenia, reduced concentrations of IgA and IgM, lysozyme and complement in the blood serum). Types II, III, and IV immunopathologic reactions to uterine tube tissue homologous antigen suggest their contribution to a chronic inflammatory process. A relationship between severity of morphologic changes in uterine tubes and degree of immunity defects and immunopathologic reaction manifestation was revealed.
Assuntos
Doenças das Tubas Uterinas/imunologia , Homeostase/imunologia , Infertilidade Feminina/imunologia , Doenças Peritoneais/imunologia , Adulto , Formação de Anticorpos , Infecções por Chlamydia/complicações , Infecções por Chlamydia/imunologia , Doença Crônica , Doenças das Tubas Uterinas/complicações , Feminino , Humanos , Imunidade Celular , Infertilidade Feminina/etiologia , Contagem de Leucócitos , Doença Inflamatória Pélvica/complicações , Doença Inflamatória Pélvica/imunologia , Doenças Peritoneais/complicações , Infecções por Ureaplasma/complicações , Infecções por Ureaplasma/imunologiaRESUMO
The incidence of chlamydial infection in women with tuboperitoneal sterility is 49.5%. Clinically inflammatory diseases of the pelvic organs in such an infection are characterized by a primary chronic course with frequent recurrences and involvement of the cervix uteri, urethra, internal genitals, with moderately manifest clinical symptoms. Therapy of chlamydial infection should be combined with individual selection of therapeutic measures with due consideration for the inflammatory process phase and site, for the type of pathologic changes, general and immune status.
Assuntos
Infecções por Chlamydia/complicações , Doenças das Tubas Uterinas/etiologia , Infertilidade Feminina/etiologia , Doenças Peritoneais/etiologia , Adulto , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , Infecções por Chlamydia/terapia , Doença Crônica , Terapia Combinada , Doenças das Tubas Uterinas/diagnóstico , Doenças das Tubas Uterinas/microbiologia , Doenças das Tubas Uterinas/terapia , Feminino , Humanos , Incidência , Infertilidade Feminina/diagnóstico , Infertilidade Feminina/microbiologia , Infertilidade Feminina/terapia , Doenças Peritoneais/diagnóstico , Doenças Peritoneais/microbiologia , Doenças Peritoneais/terapia , RecidivaRESUMO
Analysis of the diagnostic and prognostic potentialities of measurements of blood serum medium molecules and medium-molecular peptides in women with inflammatory diseases of the small pelvis organs has been carried out, involving 60 patients and 10 healthy women. Medium molecule levels were estimated with the use of Gabrielyan and Lipatova's method, the level of medium-molecular peptides by the biuret method. The results recommend these measurements to be included in the complex of clinical laboratory investigations carried out in patients with pelvic inflammations for the early detection of purulent ++tubo-ovarian inflammations, endogenous intoxication, and a more rational treatment.
Assuntos
Endometrite/diagnóstico , Doença Inflamatória Pélvica/diagnóstico , Peptídeos/sangue , Doença Aguda , Diagnóstico Diferencial , Endometrite/sangue , Feminino , Humanos , Peso Molecular , Doença Inflamatória Pélvica/sangue , Prognóstico , Valores de Referência , Índice de Gravidade de DoençaAssuntos
Algoritmos , Estágio Clínico/tendências , Ginecologia/educação , Obstetrícia/educação , Faculdades de Medicina/tendências , Adulto , Currículo , Feminino , Doenças dos Genitais Femininos/diagnóstico , Doenças dos Genitais Femininos/terapia , Ginecologia/tendências , Humanos , Obstetrícia/tendências , Gravidez , Gravidez Tubária/diagnóstico , Gravidez Tubária/cirurgia , U.R.S.S.RESUMO
This paper presents a scale for the evaluation of severity of late toxemia using 15 clinical and laboratory indices as well as central and regional hemodynamic parameters obtained by whole-body rheography and poly-rheography. Interpretation of the severity of late toxemia involved findings of functional postural and pharmacological tests. The scale is recommended for its use in the obstetric practice.