RESUMO
The side effects of shock waves on biological tissues have been intensively investigated in past years. In contrast to the morphological studies, only a little information exists up to now about molecular effects of shock waves at the DNA level. To study the shock wave effects on DNA in water solution, 100, 500, 1,000, 1,500 wave shocks, generated with a experimental lithotripter, were applied at 18 kV and 20 kV, corresponding to the positive pressure peaks of 16 and 30 MPa and negative ones of 5 and 9 MPa. The DNA damage was evaluated in two "submarine" electrophoresis approaches. In the first - standard DNA electrophoresis - no DNA damage was detected, confirming previously described results. In the second electrophoresis, performed under changed conditions, sever double strand DNA damage was found. Our results strongly suggest that shock waves applied at the therapeutical level of energy may generate the double strand DNA damage.
Assuntos
Dano ao DNA , Litotripsia/efeitos adversos , Eletroforese em Gel de Ágar , HumanosRESUMO
The authors have analysed the frequency and structure of congenital anomalies in children born in the Pomeranian district in the period from 01.07.1997 to 31.12.1998. Among a total of 28.361 births in that area, 748 (2.64%) were affected by congenital anomalies. Among 28.361 births, 620 (2.18%) were from multiple pregnancies. 23 (3.71%) among births from multiple pregnancies were affected by congenital malformations. The prevalence rate of inborn anomalies in births from multiple pregnancy in our area were higher (3.71%) in comparison to births from singleton pregnancy (2.61%). It implies that children born from multiple pregnancy are at higher risk of developing congenital anomalies.