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J Bacteriol ; 181(2): 426-33, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9882655

RESUMO

We have identified a locus essential for galacturonate utilization in Bacillus subtilis. Genes homologous to Escherichia coli and Erwinia chrysanthemi glucuronate and galacturonate metabolic genes were found in a cluster consisting of 10 open reading frames (ORFs) in the B. subtilis chromosome. A mutant of B. subtilis containing a replacement of the second and third ORFs was unable to grow with galacturonate as its primary carbon source. Galacturonate induced expression from a sigmaA-dependent promoter, exuP1, located upstream from the first ORF. The eighth ORF in this cluster (the exu locus) encodes a LacI and GalR homolog that negatively regulated expression from exuP1. A 26-bp inverted repeat sequence centered 15 bp downstream from the exuP1 start point of transcription acted in cis to negatively regulate expression from exuP1 under noninducing conditions. Expression from the exuP1 promoter was repressed by high levels of glucose, which is probably mediated by CcpA (catabolite control protein A). A sigmaE-dependent promoter, exuP2, was localized between the second and third ORFs and was active during sporulation.


Assuntos
Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Genes Bacterianos , Glucuronatos/metabolismo , Ácidos Hexurônicos/metabolismo , Aldose-Cetose Isomerases/genética , Sequência de Bases , Mapeamento Cromossômico , Cromossomos Bacterianos , DNA Bacteriano/química , DNA Bacteriano/genética , Dickeya chrysanthemi/genética , Escherichia coli/genética , Genótipo , Ácido Glucurônico , Dados de Sequência Molecular , Família Multigênica , Fases de Leitura Aberta , Regiões Promotoras Genéticas , Sequências Repetitivas de Ácido Nucleico
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