CORRELATION OF CD4+Т LYMPHOCYTES ACTIVATION WITH INTERLEUKIN IL-9, IL-17, IL- 22 PROFILES IN THE PERIPHERAL BLOOD OF PATIENTS WITH PLAQUE PSORIASIS.

Psoriasis is a T cell mediated chronic inflammatory skin disease affecting about 2% of the population worldwide. Recently has established the central role of IL-23/Th17 immune axis in the pathogenesis of psoriasis and different subclasses of T cells including Th1 and Th17 cells are involved in initiation and amplification of the skin inflammation process, in addition, in cases of recurrent psoriasis, Th22 cells play the role of memory cells with the help of Th9 cells, which are also important in this process. The main goal was to evaluate the ratio of T cell profile and IL23/Th17 axis by evaluating IL17A, IL22, IL9 in peripheral blood of persons with moderate to severe plaque psoriasis. We have estimated the activation of IL-23/Th17 axis by evaluating the level of IL-17A, IL-22 and IL-9 in peripheral blood of patients with plaque psoriasis (n=18) with different severity of the disease (PASI from 10 to 40) comparing the results with data obtained from healthy persons (n=15). The expression of CD69 activation marker on T helper cells has been evaluated as well. The results were analyzed using FACScan flow cytometer (Becton Dickinson). The percentage of CD3 + T lymphocytes in the peripheral blood of patients with psoriasis was not significantly different compared to normal healthy volunteers, however, the level of expression of CD4 + T cells was reduced. We observed a dramatic increase in IL22 along with a decrease in the level of expression of IL-9 and IL-17, the expression of Th activation marker (CD69) was also decreased in comparison with the control group. The T cell profile and the IL-23/Th17 axis functional activity levels were significantly different from the literature data obtained about the inflammatory region (psoriatic lesions on the skin). IL-9 and IL-17 expression levels are decreased in peripheral blood Th cells, which may be explained by mobilization of the corresponding Th9 and Th17 cells into the inflammatory site.

Influence of the social and economical factors on the indicators of height and weight among the pupils, residents of the city and villages in eastern Georgia (Kakheti region).

The research has been held in the city and its villages among schoolgirls and schoolboys out of them are city (n=613) and village (n=241) residents. The body height and mass, was calculated according to standard methods. Also research based on questionnaires has been held .This research determines social-economic statement, physical activities and diets of students. Statistical processing of data was done with the help of statistic method ANOVA. Data compared to WHO child grows standard percentage-charts, and to percentile diagram made for georgian children and adolescents in 2001-2003. Concerning the height indexes stunting was noticed among the village resident girls at the age 9-14 (8,9%) and 9-11 year old boys (3,8%) and it also prevails among girls that require further researches to reveal reasons. Also the amount of law height index among girls and boys is high in the cities as well as villages although it prevails in the villages, mostly among boys (30, 8%). Comparing with village residents high height index prevails among the city resident boys (18,7%) and among girls (11, 9%), and higher than 97 percentile indexes is revealed among 4,9% of boys living in the city. Mass deficiency was reveled among the city girls 2,4 % and the village resident girls 5,1%; accordingly it is prevailed among girls living in the villages. Mass deficiency among the boys was only revealed among the city residents and it was 3,9 %. According to our data, decrease of the body mass average index is fixed among girls but among the boys it increase. and among 2,4% of girls. So village resident girls are shorter and thinner, but boys are short and overweight, comparing with the city residents. Research based on questionnaires showed that city resident school children food ration, whose social-economic condition is better, is rich with proteins, when majority of village residents food ration lack proteins and mostly consist of those products which are rich of carbohydrates. According to surveys low physical activity was revealed among city as well as village resident school children, especially in boys. Average height indexes based on our researches have been compared to percentile diagrams for children and adolescents of Georgia , based on researches held in 2001-2003. The average height indexes of the students born in 2000 and following years (including from 6 - 13 years) are located between the percentage indexes 50-90 established for Georgian children, but at the ages 14-18 between 10-50 which indicates to the tendency of increasing height of schoolchildren in the city and villages During the last years, tendency in the increasing of height is fixed in India and Sri Lanka.

15-year history of spontaneous appendico-vesical fistula (case report).

Appendicovesical (AVF) fistula is rare complication in the modern era. Only a few cases of AVF were described in the literature of the last decade. AVF is meanly associated with a history of appendicitis. The guiding symptoms are coprosuria and pneumaturia, with recurrent infection of the urinary tract. The accurate pre-operative diagnosis is difficult to make. It usually took at least one year from the onset of symptom to confirm diagnosis. It has been reported a few cases of 15 year history of undiagnosed AVF. We represent a case of EVF, spontaneously developed after treated acute appendicular colic presenting an unusual long - 15 years undiagnosed history, without presenting of typical symptoms. The analyses of disease history after cystoscopy lead to correct pre-operative diagnosis. AVF can develop as a complication not only after untreated appendicitis, but also after "successfully" treated with antibiotics appendicular colic may indicate the presence of an AVF and needs to assessing patient's history and applying cystoscopy and modern examination methods in complex diagnosis issues.