Increased safety in periodontal surgery: Doppler ultrasound for detection of relevant palatal blood vessels-A proof-of-concept and cross-sectional study.

AIM: To evaluate the suitability of a Doppler ultrasound probe in detecting the greater palatine artery or its greater branches non-invasively. MATERIALS AND METHODS: The palatal mucosa of 108 participants (median age 34 years, 51 female) was systematically divided into transversal sectors, each aligning with the positions of the upper molars (M), premolars (P) and canine teeth (C), aiming to facilitate precise and consistent localization of the detected palatal blood vessel across different patients. Blood flow of the palatal blood vessels, presumably, was located by scanning the palatal vault bilaterally using an 8-MHz ultrasound probe linked to a transducer. The distance to the corresponding tooth was measured using a millimetre-scale periodontal probe. RESULTS: Within the regions of M2 to P1, the ultrasound transducer gave a delimitable acoustic pulse signal in 80%-98% of all measurements. The measured median distances between the determined position of the artery and the corresponding teeth ranged from 13 to 15 mm, with smaller distances in the anterior region. In several sectors, the distance was significantly higher for men (C: p = .048; P1: p = .041, M1: p < .01; M2: p = .034). CONCLUSIONS: Use of the Doppler ultrasound transducer might be a promising approach to non-invasively detect relevant palatine blood vessels preoperatively. It, therefore, might have the potential to reduce the risk of accidental injury during palatal surgery.

Changes in serum omega fatty acids on a Mediterranean diet intervention in patients with gingivitis: An exploratory study.

BACKGROUND: Omega-6 and omega-3 polyunsaturated fatty acids (PUFAs) are precursors of pro- and anti-inflammatory lipid mediators. Serum PUFA levels could influence the severity of inflammatory oral diseases, such as gingivitis. OBJECTIVE: The study analyzed serum PUFA levels in a six-week randomized controlled trial in individuals on the Mediterranean diet (MedD), associations with the intake of specific foods, and possible correlations with oral inflammatory parameters. METHODS: Data from 37 study participants on either a MedD (MedDG; n = 18) or a "Western diet" in the control group (CG, n = 19) were analyzed. Dental examinations and serum analyses were performed at two time points, T1 (baseline) and T2 (week 6). Serum PUFA status, adherence to the MedD, and data from a Food Frequency Questionnaire were analyzed. RESULTS: Within the MedDG omega-6 fatty acid levels decreased significantly. In the overall sample, the proportional decrease in sites with bleeding on probing correlated weakly to moderately with the decrease in total omega-6 fatty acid level (Spearman's ρ = 0.274) and the decrease in gingival index correlated moderately with the decrease in linoleic acid level (Spearman's ρ = 0.351). Meat and fast-food consumption correlated positively with levels of various omega-6 fatty acids, whereas nut, fish, and dairy product consumption correlated positively with omega-3 levels. CONCLUSION: Adherence to a MedD was associated with a decrease in serum omega-6 levels, which positively affected the omega-6/omega-3 ratio. The MedD associated reduction in serum omega-6 levels may be a mechanism that favorably affects gingival inflammatory parameters.

Human Primary Odontoblast-like Cell Cultures-A Focused Review Regarding Cell Characterization.

Cell cultures can provide useful in vitro models. Since odontoblasts are postmitotic cells, they cannot be expanded in cell cultures. Due to their extension into the dentin, injuries are inevitable during isolation. Therefore, "odontoblast-like" cell culture models have been established. Nowadays, there is no accepted definition of odontoblast-like cell cultures, i.e., isolation, induction, and characterization of cells are not standardized. Furthermore, no quality-control procedures are defined yet. Thus, the aim of this review was to evaluate both the methods used for establishment of cell cultures and the validity of molecular methods used for their characterization. An electronic search was performed in February 2022 using the Medline, Scopus, and Web of Science database identifying publications that used human primary odontoblast-like cell cultures as models and were published between 2016 and 2022. Data related to (I) cell culture conditions, (II) stem cell screening, (III) induction media, (IV) mineralization, and (V) cell characterization were analyzed. The included publications were not able to confirm an odontoblast-like nature of their cell cultures. For their characterization, not only a similarity to dentin but also a distinction from bone must be demonstrated. This is challenging, due to the developmental and evolutionary proximity of these two tissue types.

Retrospective Clinical Evaluation of Subgingival Composite Resin Restorations with Deep-Margin Elevation.

PURPOSE: To evaluate the long-term clinical quality of subgingivally placed composite resin restorations and the inflammatory status of surrounding supracrestal gingival and periodontal tissues. MATERIALS AND METHODS: Patients with at least one subgingival restoration with deep-margin elevation placed between 2010 and 2020 at Heidelberg University Hospital and Tübingen University Hospital were identified. A sound tooth was used as control. Intraoral examination including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival bleeding index (GBI), and plaque control record (PCR) was conducted. The clinical quality of the restorations was evaluated using the modified FDI criteria. For comparison between control and test teeth, a logistic mixed-effects model was used for GBI, PCR, and BOP, while a linear mixed-effects model was used for CAL. Multivariable linear and logistic regressions were used to examine the influence of smoking, age of restoration, number of decayed, missing and filled teeth, use of interdental brushes, and CAL. RESULTS: Sixty-three patients were included in the study. The mean age of the restorations was 2.70 ± 1.90 years. There were no significant differences between test and control teeth with respect to inflammatory parameters BOP, GBI, and PCR. CAL was significantly higher in test teeth than in controls (p = 0.027). The regression models revealed that CAL has a significant influence on GBI (p = 0.008) and BOP (p < 0.001). A significantly increased GBI occurred especially on test teeth in patients who did not use interdental brushes daily (p = 0.010). The clinical quality of restorations was rated excellent or good in 70%, an no restoration was rated unacceptable. CONCLUSION: No increased inflammation was observed on sites with subgingivally placed composite restorations over an observation period of approximately 3 years. Regular interdental brush use was associated with less gingival inflammation.

Evaluation of five methods to identify composite restorations in human teeth on a forensic purpose-an ex vivo comparative study.

The use of tooth-colored composite resin materials in dentistry is increasing. As composite restorations appear similar to the natural tooth structure, their detection is becoming more challenging. The aim of this study was to compare five diagnostic methods for detection of dental restorations and to create reliable postmortem dental records for forensic purposes. A conventional examination method without a direct light source (CONV), with a dental loupe light (DL), Galilean loupes with a direct light source (GDL), a fluorescence-inducing device (FIT), and intraoral radiographs (RX) were compared. Dentists specializing in conservative dentistry or oral surgery and dentistry students participated. Regarding sensitivity, specificity, positive predictive value, and negative predictive value, FIT and RX showed significantly higher results than CONV, DL, and GDL. RX and FIT showed comparable results in identifying composite restorations but depending on their location. The combination of both methods may lead to even better results. In conclusion fluorescence-inducing devices show good results in identifying composite restorations and therefore should be considered as a standard tool in forensic examinations. Another advantage is their small size, low cost, and mobility. Good illumination and magnification devices are recommended to enhance performance during conventional examinations. Involving dentists specialized in conservative dentistry could contribute to dental identification.

Detection and Removal of Tooth-Colored Composite Resin using the Fluorescence-Aided Identification Technique.

The detection and removal of tooth-colored filling materials is a major challenge for every dentist. The Fluorescence-aided Identification Technique (FIT) is a noninvasive tool to facilitate the distinction of composite resin material from sound tooth substance. Compared to conventional illumination, FIT is a very accurate, reliable, and fast diagnostic method. When composite resin is illuminated with a wavelength of approximately 398 ± 5 nm, certain fluorescent components make the composite resin appear brighter than the tooth structure. Any fluorescence-inducing light source with the appropriate wavelength can be used for this method. Optimally, this technique is used without additional natural or artificial lighting. The application of FIT can be used for diagnostic purposes, for example, dental charts, and additionally for the complete and minimally invasive removal of composite resin restorations, bracket debonding, and trauma splint removal. The assessment of volumetric changes after composite removal can be provided by overlapping pre- and postoperative scans and subsequent calculation using suitable software.

Correction: Bartha et al. How to Measure Adherence to a Mediterranean Diet in Dental Studies: Is a Short Adherence Screener Enough? A Comparative Analysis. Nutrients 2022, 14, 1300.

The authors would like to make a correction in a recently published paper [...].

How to Measure Adherence to a Mediterranean Diet in Dental Studies: Is a Short Adherence Screener Enough? A Comparative Analysis.

This study aimed to evaluate the Mediterranean Diet Adherence Screener (MEDAS) in a study investigating the anti-inflammatory effect of a 6-week Mediterranean diet intervention on periodontal parameters. Data from a randomized clinical trial were analyzed for correlations between the MEDAS score and oral inflammatory parameters (bleeding on probing (BOP), gingival index (GI), and periodontal inflamed surface area (PISA)) and select nutrient intakes estimated by a food frequency questionnaire (FFQ) and a 24-h dietary recall (24dr). A mixed model, calculations of Spearman ρ, Lin's Concordance Coefficient (CC), and Mann-Whitney U test were used for the statistical analyses. The MEDAS score was significantly negatively correlated with periodontal inflammation (BOP: CoE −0.391, p < 0.001; GI −0.407, p < 0.001; PISA −0.348, p = 0.001) and positively correlated with poly unsaturated fatty acids/total fat, vitamin C, and fiber intake estimates obtained from the FFQ and 24dr (ρ 0.38­0.77). The FFQ and 24dr produced heterogeneously comparable intake results for most nutrients (CC 0­0.79, Spearman ρ 0.16­0.65). Within the limitations of this study, the MEDAS was able to indicate nutritional habits associated with different levels of periodontal inflammation. Accordingly, the MEDAS can be a sufficient and useful diet screener in dental studies. Due to its correlation with oral inflammatory parameters, the MEDAS might also be useful in dental practice.

A quantitative assessment of silicone and PTFE-based stamp techniques for restoring occlusal anatomy using resin-based composites.

OBJECTIVES: Publications on stamp techniques for placing resin-based composite (RBC) restorations consist mainly of case studies. Furthermore, comparative studies are rare and no longer relevant to the materials tested today. Thus, two general techniques were investigated in this study. MATERIALS AND METHODS: Standardized occlusion class I cavities were prepared in twenty-eight extracted caries-free wisdom teeth with unimpaired occlusal surfaces and restored with the RBC material Grandio®. Light curing of the final layer was performed either after removal of the stamp isolated with PTFE tape or by leaving a stamp made of transparent polysiloxane in place. CEREC scans of the RBC restorations placed (follow-up) were superimposed on scans of the unimpaired occlusal surface (baseline) and quantitatively analyzed with the software OraCheck with regard to volume change and gain or loss of layer thickness in six sectional planes. RESULTS: Assessing the excess material, there was no difference (p = 0.31) between the silicone technique (0.26 mm ± 0.02) and the PTFE technique (0.22 mm ± 0.02 mm). Nevertheless, the loss of tooth substance was significantly greater (p < 0.001) with the silicone technique (-0.29 mm ± 0.02 mm) than with the PTFE technique (-0.15 mm ± 0.02 mm). CONCLUSIONS: With the PTFE stamp technique, less healthy tooth structure was removed during the finishing procedure and the stamp was more dimensionally stable. CLINICAL RELEVANCE: The study shows the advantages and disadvantages of the investigated stamp techniques and helps the practitioner to choose an appropriate technique.

Effect of the Mediterranean diet on gingivitis: A randomized controlled trial.

AIM: This study aimed to investigate the effects of a 6-week Mediterranean diet (MD) intervention on gingival inflammatory and anthropometric parameters of patients with gingivitis. MATERIALS AND METHODS: Forty-two participants were allocated to MD group (MDG) or control group (CG). After a 2-week equilibration period regarding dental care procedures, only MDG changed their diet to MD for 6 weeks, supported by a diet counselling. Gingival and anthropometric parameters were assessed at baseline (T0), Week 2 (T1, beginning of MD intervention), and Week 8 (T2). Adherence to MD was assessed by the Mediterranean Diet Adherence Screener (MEDAS); dietary behaviour was evaluated by the German Health Interview and Examination Survey for Adults Food Frequency Questionnaire (DEGS-FFQ). RESULTS: Plaque values remained constant in both groups. Inflammatory periodontal and anthropometric parameters decreased in the MDG only (gingival index: T1 1.51 ± 0.21, T2 1.49 ± 0.24; bleeding on probing: T1 51.00 ± 14.65, T2 39.93 ± 13.74; body weight: T1 79.01 ± 15.62, T2 77.29 ± 17.00; waist circumference: T1 84.41 ± 10.1, T2 83.17 ± 10.47 (p < .05). MEDAS revealed a sufficient diet adherence for MDG. CONCLUSION: Within this study, gingival inflammatory parameters were significantly reduced by MD, whereas plaque parameters remained constant. The diet counselling achieved sufficient adherence with beneficial changes in weight loss and waist circumference.

How well can today's tooth-colored dental restorative materials reproduce the autofluorescence of human teeth? - Ambition and reality!

OBJECTIVES: The autofluorescence of dental hard tissues has been known for over 100 years. Thus, manufacturers add fluorophores to dental restorative materials to improve the esthetic properties of these materials. So far, there has been no study evaluating the ability of these fluorophores to reproduce the autofluorescence of dental hard tissues. MATERIALS AND METHODS: A total of 240 different color shades representing 17 different brands of fluorescent light-curing RBC and CAD/CAM restorative materials were analyzed with a monochromator-based microplate reader. Additionally, combined enamel-dentin specimens (n = 11) were analyzed as "gold standard". The total fluorescence (TF) and the physiologically relevant luminous efficiency function adjusted total fluorescence (TFa ) were determined. The differences between the brands and the enamel-dentin specimens were further evaluated and visualized as contour plots. RESULTS: Merely the TFa of the brands CERASMART™, Filtek Supreme XTE™, KZR-CAD HD 2, and LuxaCam composite were not significantly different to the enamel-dentin specimens. The analysis of the contour plots revealed that even these four materials showed a fluorescence excess for the excitation wavelengths below about 400 nm and a deficit above this wavelength. CONCLUSION: None of the materials analyzed in this study were able to reproduce the natural fluorescence spectrum of the enamel-dentin specimens. CLINICAL SIGNIFICANCE: Unlike the statements and images of blue fluorescent materials in the manufacturers' brochures, none of the materials examined here is fully capable of reproducing the natural autofluorescence of teeth.

Restorative CAD/CAM materials in dentistry: analysis of their fluorescence properties and the applicability of the fluorescence-aided identification technique (FIT).

OBJECTIVES: This study investigated the fluorescence properties of the most commonly used fluorescent CAD/CAM materials for monolithic dental restorations and their suitability to perform the fluorescence-aided identification technique (FIT). MATERIALS AND METHODS: A total of 175 different color shades (n = 1) from 13 CAD/CAM material brands were analyzed with a monochromator-based microplate reader. Additionally, dentin, enamel, and combined dentin-enamel specimens (respectively, n = 11) were analyzed for comparison purposes. The maximum fluorescence intensity, the corresponding excitation and emission wavelength, and the total fluorescence for the wavelength spectrum λex = 395 nm - 415 nm used for FIT were determined. RESULTS: All assessed CAD/CAM ceramics showed virtually no total fluorescence for the wavelength spectrum λex = 395 nm - 415 nm used for FIT. CERASMARTTM, KZR-CAD HD 2, and LuxaCam Composite displayed total fluorescence values similar to that of the tooth hard substances. All other resin-based CAD/CAM materials showed a significantly higher total fluorescence than the tooth hard substances. CONCLUSIONS: Apart from the mentioned exceptions, all CAD/CAM materials assessed could be suitable for the FIT, either because they are more fluorescent than hard tooth substances or because they do not fluoresce at all at the respective wavelength of λex = 395 nm - 415 nm. CLINICAL RELEVANCE: This study provides insight into the not yet well-known fluorescent properties of dental CAD/CAM materials. This knowledge is not only necessary to reproduce the fluorescence properties of natural teeth but also for the applicability of diagnostic fluorescence inducing techniques.

Reentry of endodontic access cavities: composite residue and loss of tooth substance.

The purpose of this study was to investigate the ability of dentists to remove composite fillings from endodontic access cavities using illumination from a conventional light source (CLS) versus the fluorescence-aided identification technique (FIT) in terms of completeness, selectivity and treatment duration. Therefore, two independent operators removed composite resin from six sets of root-filled incisors in a maxillary model under simulated clinical conditions using the CLS or FIT method (twelve teeth per operator and technique). The duration of treatment was recorded and before-after micro-CT scans were superimposed for volumetric assessment of treatment completeness and selectivity. Statistical significance was determined by t-testing and two-way ANOVA for operator comparison. Overall, there was no significant difference between FIT and CLS in terms of volume, height and area of composite residues (p=0.98 / p=0.75 / p=0.64) and regarding hard tissue loss in terms of volume, depth and area (p= 0.93 / p= 0.70 / p= 0.14). However, there was a significant difference between the two groups regarding treatment time (FIT= 428s, CLS=523s; p=0.023). Significant differences between operators regardless of method were found for volume, height and area of composite residues (p<0.05) and also for defect area (p=0.01) and time (p<0.001). Significant differences between operators including the method was only found for height of composites (p=0.037). It can be concluded, that composite remnants and tooth structure losses may occur after reentry of root-filled teeth regardless of the illumination method (conventional vs. fluorescence-aided) and operator, but preparation was less time-consuming with FIT.

The fluorescence of resin-based composites: An analysis after ten years of aging.

The long-term preservation of fluorescence qualities of resin-based composite (RBC) restorations is an absolute condition for the implementation of the fluorescence-aided identification technique (FIT) in dentistry and forensic medicine. Therefore, this study assessed the fluorescence of 244 color shades of 16 commercially available RBC brands with a monochromator-based multimode microplate reader. The specimens were stored in the dark at room temperature and reassessed ten years after the initial investigation. The mean intensity of the fluorescence maxima decreased from (31,030±936) RFU to (22,027±632) RFU. Linear regression resulted in r2=0.972 and a slope=0.701±0.005. The fluorescence intensity of the tested RBCs dropped to about 70% of the initial intensity independent of the brand, color shade and initial fluorescence intensity. On the basis of this in vitro 10-year data set, we assume that in vivo RBC fluorescence is also suitable for the detection and differentiation of clinically aged RBC restorations by FIT.

User-friendly restorative approach combining shaping indices and PTFE-based stamp techniques: a feasibility example performed by an undergraduate dental student in a dentition damaged by caries and tooth wear.

The rehabilitation of patients with wear on multiple teeth, in which a recovery of the occlusal/incisal vertical dimension is required to acquire satisfactory levels of functionality and esthetics, is often a difficult procedure even for experienced dental practitioners. Based on wax-ups simulating an ideal structural/functional reconstruction of the teeth, the use of shaping indices is a reliable way to plan predictable patient-oriented results when using resin-based composites. The proposal to combine shaping indices with PTFE-based stamp techniques also helps clinicians to easily achieve satisfactory modeling results giving them the unique opportunity to eliminate the presence of composite over-contours before polymerization. By using PTFE-based stamps the requirement to use conventional, strictly transparent indices becomes superfluous. In a clinical case with multiple caries lesions and tooth wear, the advantage of several simple operative measures including PTFE-based stamp procedures are presented as a feasibility example of this noninvasive or minimally invasive restorative approach performed by an undergraduate dental student.

Minimally invasive removal of tooth-colored restorations: evaluation of a novel handpiece using the fluorescence-aided identification technique (FIT).

OBJECTIVES: This study investigated the ability of the fluorescence-aided identification technique (FIT) facilitated by a novel handpiece to simplify the removal of tooth-colored composite restorations with water-cooled rotating instruments. MATERIALS AND METHODS: Five undergraduate students and five dentists (6-14 years of professional experience) were asked to remove dental restorations in vitro using both the conventional technique (CT) and the fluorescence-aided identification technique. The FIT method was performed on teeth restored in addition to the fluorescent composite resin with the non-fluorescent (FIT1) and fluorescent (FIT2) bonding agent. CEREC scans were superimposed and three-dimensionally analyzed with the software OraCheck 2.13 with respect to the cavity surface area still covered with composite resin and the volume of the needlessly removed sound hard tissue. Additionally, the removal procedure was timed. RESULTS: The FIT2 group showed the most promising results: the smallest cavity surface area covered by composite resin independent of the professional expertise, and for the dentist group, the smallest amount of removed sound hard tissue and the fastest removal. CONCLUSIONS: Using the fiber optic of the handpiece for fluorescence excitation has been proven to be effective for performing the FIT, and therefore, to improve the removal of tooth-colored restorations. CLINICAL RELEVANCE: This study is basic research to encourage the integration of fluorescence inducing light sources in dental treatment units by the manufacturers as a prerequisite for a simplified daily use of the FIT.

Evaluation of a Fluorescence-aided Identification Technique (FIT) to assist clean-up after orthodontic bracket debonding.

OBJECTIVES: To compare a fluorescence-aided identification technique (FIT) with a conventional light source (CLS) for removing composite during debonding of brackets with respect to time needed, composite remnants, and tooth substance loss. MATERIALS AND METHODS: Twelve maxillary models with 10 bovine teeth each were digitally surface-scanned and metal brackets were bonded on each tooth with Opal Seal and Opal Bond. Two operators: an experienced orthodontist (A) and an undergraduate student (B) received six models each and were asked to remove the composite remnants with a tungsten carbide bur and Sof-Lex discs by both a conventional light source (CLS group, n = 3), and fluorescent inducing light (FIT group, n = 3). The time taken was recorded, and a postoperative scan was digitally superimposed on the preoperative scan to quantify number of teeth with composite remnants and volume and thickness of enamel loss and composite remnants. Chi-square test and independent t-tests were performed to compare methods with a significance level of 5%. RESULTS: Compared to CLS, both operators needed significantly less time when using the FIT method and degree of enamel loss, height, and volume of composite remnants and total remaining composite remnants were significantly reduced. By FIT, the volume of enamel loss was significantly reduced for operator A only. Operator B removed the same enamel volume with either method. CONCLUSIONS: Cleanup after orthodontic debonding with the FIT was superior regarding time needed and removal of composite remnants. Total enamel loss reduction was operator-dependent.

A new Fluorescence-aided Identification Technique (FIT) for optimal removal of resin-based bracket bonding remnants after orthodontic debracketing.

Tooth-colored resin-based bracket bonding agents are used to fix orthodontic brackets to teeth as inconspicuously as possible. However, after debonding the brackets, these tooth-colored bonding remnants remain on the tooth surface and must be removed to prevent adverse sequelae. Various techniques can be used, all of which cause varying damage to the tooth structure, as the transition between composite and enamel is visually difficult to detect. With the fluorescence-aided identification technique (FIT) presented here, the fluorescent properties of new orthodontic composites are used for visualization, thereby allowing the tooth-colored but fluorescent resin-based bracket bonding remnants to be removed under direct view quickly and without damaging the tooth.

Evaluation of a Fluorescence-aided Identification Technique (FIT) for removal of composite bonded trauma splints.

BACKGROUND/AIM: Accurate removal of composite bonded titanium trauma splints (TTS) can be challenging. The aim of this study was to evaluate a Fluorescence-aided Identification Technique (FIT) compared with a conventional light source (CT) for removal of trauma splints regarding loss of tooth substance, residual composite, and time taken. MATERIALS AND METHODS: Twenty maxillary models with six bovine teeth (13 - 23) were fabricated. An optical three-dimensional impression was taken and a TTS was applied from 12 to 22 with two bonding sites on every tooth under standardized conditions using an etch-and-rinse adhesive and resin composite. Two operators removed 10 splints each (5x FIT and 5x CT). For FIT, a 405 nm light-emitting headlamp was used. Time was recorded. A post-operative scan was superimposed on the pre-operative scan using OraCheck software. A qualitative and quantitative analysis of tooth defects and composite remnants was performed by two operators and statistically analyzed. RESULTS: Compared with the CT, FIT led to significantly fewer and smaller enamel defects (mean volume: -0.04 mm3 vs -0.33 mm3 ) (P < 0.001), significantly less composite remained (mean volume: 0.02 mm3 vs 0.28 mm3 ) (P < 0.001), and the removal procedure with FIT was significantly quicker (mean: 162 seconds vs 268 seconds) (P < 0.001), CONCLUSIONS: FIT facilitated the removal of composite used to bond trauma splints leading to less time-consuming as well as less invasive treatment. It left fewer composite residues on enamel surfaces.

Integrity testing of a smooth surface resin sealant around orthodontic brackets using a new Fluorescence-aided Identification Technique (FIT).

OBJECTIVE:: To investigate the integrity of a fluorescing resin-based sealant placed around orthodontic brackets using the Fluorescence-aided Identification Technique (FIT). MATERIALS AND METHODS:: Standard brackets were bonded to the buccal surfaces of 17 extracted sound permanent premolar crowns sealed with ProSeal®. Specimens were thermocycled (20,000 cycles, 5-55°C), and toothbrushing was simulated using an electric toothbrush and artificial aqueous toothpaste slurry. Changes in the sealed area were measured after one, two, three, and four alternating thermocycling-brushing cycles simulating 2 years of wear. Digital images were captured applying FIT (405 nm) using a digital camera-equipped stereomicroscope. ImageJ was used to measure sealant integrity and loss. RESULTS:: There was a time-dependent decrease in sealed areas by between 21% and 100% (mean 54%). The sealant lost its integrity immediately after the first cycle, and unfilled areas were observed in all samples. CONCLUSIONS:: The analyzed sealant lost its integrity over time. Using the proposed FIT, sealed surfaces were easily verified and quantified.