RESUMO
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.
Assuntos
Agrobacterium tumefaciens/genética , Cromossomos Artificiais Bacterianos/genética , DNA de Plantas/genética , Genoma de Planta , Magnoliopsida/genética , Southern Blotting , Clonagem Molecular , DNA de Plantas/análise , Escherichia coli/genética , Vetores Genéticos , Reação em Cadeia da Polimerase , Transformação GenéticaAssuntos
Clonagem Molecular/métodos , Impressões Digitais de DNA/métodos , Polimorfismo Genético/genética , Eletroforese em Gel de Poliacrilamida/métodos , Genes de Plantas/genética , Marcadores Genéticos/genética , Reação em Cadeia da Polimerase/métodos , Coloração pela Prata/métodos , Glycine max/genéticaRESUMO
We have determined the length of early noduline gene ENOD12 in various varieties and lines of pea (Pisum sativum) using the polymerase chain reaction (PCR). It was demonstrated that promoter regions of ENOD12A and ENOD12B genes in line 2150 (Afghanistan) are longer than these in variety "Feltham first". The disparity is 14 bp. When studying these genes in 7 different lines and varieties of pea it was found that ENOD12A gene is more variable in size than the ENOD12B gene. We showed the possibility to analyze the heritage of ENOD12 gene's alleles by using the PCR method.