[THE IDENTIFICATION OF SIX CLASSES OF PSYCHOACTIVE SUBSTANCES IN VARIOUS OBJECTS USING TECHNIOUE OF IMMUNE CHROMATOGRAPHY].

The immune chromatographic test-systems were developed to detect psychoactive substances of six classes: opiates, amphetamines, cannabinoids, ecstasy, benzodiasepines, methadone. for the first time the simple techniques of preparation of samples were developed to fast detect the mentioned substances in such biological and non-biological objects as sweat fatty discharges, hair, urine, surface lavages, parts of plants, unknown substances. The presented techniques of preparation and analysis of samples require no special equipment and reagents. They can be applied in the "field" or domestic conditions by laymen for preliminary screening of psychoactive substances.

[Comparative analysis of amphetamine identification by immunoenzyme assay and gas chromatography with mass spectrometric detection].

The objective of this study was to simultaneously identify amphetamine in biological materials by enzyme-linked immunosorbent assay (ELISA) and gas chromatography with mass spectrometric detection (GH/MS) for the verification of diagnostic value of the proposed ELISA technique. Urine samples from suspected amphetamine and related drug users were available for analysis. It is concluded that the new modification of ELISA can be recommended for application in narcological practice and for the purpose of forensic medical examination.

[The study of influence of chitosan on clinical, metabolic and immune parameters in patients with cardiovascular diseases].

The investigation of influent of antiatherosclerotic diets with chitosan on clinical and metabolic parameters in patients with cardiovascular diseases. Results of the study show that enrichment of a diet with chitosan in patients with ishemic heart disease and hypertension improved clinic, immune status, antropometric levels and lipid spectrum of blood. The research has shown, that the use in the treat-preventive purposes chitosan is rather perspective.

[ELISA-based determination of natural antibodies to endothelial NO-synthase in human blood and tear]. / Opredelenieestestvennykh antitel k éndotelial'noi NO-sintaze b krovi i sleze cheloveka metodom immunofermentnogo analiza.

Research of natural antibodies (NAA) is a new trend in immunohistochemistry. NAA shape up, in biological fluids, as normal human endogenous low- and high-weight substances, e.g. angiotensin 2, bradykinin and others, and play the regulatory role in homeostasis. Endothelial NO-synthase (e-NOS) is a key enzyme in the path of NO shaping. An e-NOS alteration is important in the pathogenesis of diabetic microangiopathy including retinopathy. We determined, within our case study, the level of NAA to e-NOS in blood and tears of patients with diabetic retinopathy and in donors. The level of NAA to e-NOS was found to be twice higher in tears versus blood in all groups of patients. It depended on specific features and complications of diabetes mellitus.

[Diagnostic implications of detecting antibodies to angiotensin-converting enzyme and its substrates]. / Diagnosticheskaia vozmozhnost' opredeleniia antitel k angiotenzinprevrashchaiushchemu fermentu i ego substratam.

The results of the analysis of diagnostic significance of examination of natural antibodies (Nab) to agiotensin-converting enzyme (ACE) and its substrates in the serum of hypertensive patients indicate that concentration of Nab to ACE differ from this mean concentration in donors. An elevated level of Nab to ACE may be considered as a compensatory reaction to increased content of the enzyme in vascular endothelium and blood flow. The same patients were examined for antibodies to peptide angiotensin II (AT-II). Enzyme immunoassay has shown that a significantly elevated level of antibodies to AT-II was only in 5 examinees. The same patients had also high Nab to ACE. The study of a group of ischemic heart disease patients with adverse effects attributed to bronchial affection treated with enalapril and diroton (ACE inhibitors) demonstrates that deterioration of cough and external respiration function is not related to exacerbation of existing chronic pulmonary inflammation. None of the patients had elevated body temperature or inflammatory changes in the blood, other signs of inflammation. Enzyme immunoassay also proved that the initial level of Nab equaled mean value for donors or was insignificantly lower. Blood serum patients with side effects contained a significantly (p < 0.02) elevated quantities of Nab to bradikinin vs initial values. Thus, the proposed method of solid phase enzyme immunoassay quantifies Nab to ACE and its substrates in the patients.

[Bronchoconstriction in administration of ACE inhibitors]. / Bronkhokonstriktsiia pri primenenii ingibitorov angiotenzinprevrashchaiushchego fermenta.

The aim of the study was assessment of bronchial capacity in the treatment with ACE inhibitors. 92 patients with essential hypertension stage I-II (mean age 52 years), ischemic heart disease and bronchial asthma (a mild, moderate and severe course) in a relative remission were treated for arterial hypertension with ACE inhibitors enalapril and diroton. Enalapril improved cough indices by 38.3%, diroton--by 13.3%. This was accompanied by a rise in the level of natural antibodies to bradikinin.

[Effect of an anti-atherogenic diet, including soy protein products with various levels of phytoestrogens, on indicators of humoral immunity in patients with hypertension and ischemic heart disease]. / Vliianie antiaterogennoi diety, vkliuchaiushchei soevye belkovye produkty s razlichnym soderzhaniem fitoéstrogenov, na pokazateli gumoral'nogo immuniteta u bol'nykh gipertonicheskoi bolez'niu i ishemicheskoi bolezn'iu serdtsa.

The dynamic of level of natural antibodies to factors of blood coagulation system (thrombin, antithrombin III, alpha 2-macroglobulin), to angiothensinogen and to noradrenaline in 95 patients with ischemic heart disease and hypertension was studied in antiatherosclerotic diet and diet with soy-protein (soy protein isolate and soybean flour). Universal normalizing effect of the diet with soy-protein consist in increase of levels of natural antibodies to thrombin, antithrombin III, alpha 2-macroglobulin, angiothensinogen and to noradrenaline.

[Immunoenzyme analysis of natural antibodies to endogenous bioregulators in patients with systemic lupus erythematosus]. / Immunofermentnyi analiz estestvennykh antitel k éndogennym bioreguliatoram u bol'nykh sistemnoi krasnoi volchankoi.

Serum levels of autoantibodies to endogenous bioregulators (prostaglandin F2 alpha, angiotensin II, epinephrine, bovine serum albumin, dinitrophenol) were measured in patients with systemic and integumental lupus erythematosus and donors and the diagnostic significance of deviations of these levels from the norm was evaluated. A total of 75 patients with lupus erythematosus aged 19-54 years with disease lasting for 0.5 to 18 months were examined. Significant differences between patients and donors were observed as regards virtually all parameters except IgG to angiotensin II.

[Change of natural antibody levels in patients with cardiovascular diseases by the use of anti-atherogenic diets with processed soy product foods]. / Izmenenie urovnia estestvennykh antitel u bol'nykh s serdechno-sosudistymi zabolevaniiami pod vliianiem antiaterogennykh diet s vkliucheniem produktov pererabotki soi.

It was investigated the influence of a diet supplemented with soy oil and soy protein on dynamic of clinic manifestation and immune status patients with IND and HBP. The results of investigations indicated that a (?).

[Chemiluminescent immunoenzyme method for determining gidazepam]. / Khemiliuminestsentnyi immunofermentnyi metod opredeleniia gidazepama.

Competitive solid-phase enzyme immunoassay of gidasepam (a benzodiazepine) in the urine with chemiluminescent detection has been developed. The sensitivity of analysis for gidasepam is 10 ng/ml. Comparative measurements of benzodiazepines in the urine of patients abusing these agents by polarization fluorescence and the proposed method showed good coincidence of the results. The results demonstrate the possibility of using this method in medicine and narcology in combination with other instrumental methods.

[Effects of anti-atherosclerotic low-sodium diet on dynamics of natural antibodies to angiotensin II, bradykinin and vasopressin in blood of patients with hypertension and obesity]. / Vliianie protivoateroskleroticheskoi giponatrievoi diety na dinamiku urovnia estestvennykh antitel k angiotenzinu II, bradikininu i vazopressinu v syvorotke krovi bol'nykh gipertonicheskoi bolezn'iu i ozhireniem.

The dynamic of natural antibodies against angiotensin II, bradykinin and vasopressin in blood serum was studied in 75 patients with hypertension and obesity. Universal normalizing effect of the diet consists in decrease of levels of natural antibodies was found.

[Autoantibodies to vasoactive peptides and angiotensin converting enzyme in patients with systemic diseases of the connective tissue]. / Autoantitela k vazoaktivnym peptidam in angiotenzinprevrashchaiushchemu fermentu u bol'nykh s sistemnymi zabolevaniiami soedinitel'noi tkani.

AIM: To estimate the level of natural autoantibodies (NAAb) to angiotensin-converting enzyme (ACE) and endogenic mediators affecting vascular tone (bradykinin--BK, angiotensin II--AII, vasopressin--VP) as well as the activity of serum ACE in patients with systemic diseases of the connective tissue. MATERIAL AND METHODS: Levels of NAAb were measured by enzyme immunoassay in sera from 30 patients with SLE, 19 patients with rheumatoid arthritis (RA) and 36 patients with scleroderma systematica (SS). Serum from donors served control. IgM NAAb to ACE were measured by a new technique. Serum ACE activity was determined by the initial velocity of hydrolysis reaction using spectrofluometry. RESULTS: IgM NAAb were detected in the sera of both patients and donors. SS patients had the level of NAAb to ACE in diffuse form significantly higher than in limited (p < 0.05). In SLE and SS patients ACE activity was significantly lower (p < 0.05) than in healthy subjects and RA patients. Levels of NAAb to BK was significantly elevated (p < 0.01) in patients with SLE and RA vs donors while to AII in SS patients it was lowered (p < 0.001). Patients with diffuse SS had NAAb to BK higher than patients with SS limited form (p < 0.01). In SLE the lowest levels of NAAb to all the mediators studied were observed in patients with nephritis, for NAAb to VP the differences were significant (p < 0.05). In patients with urinary syndrome concentration of NAAb to BK was significantly higher (p < 0.01), differences between their levels in patients with nephritis and urinary syndrome were also significant (p < 0.05). CONCLUSION: Further studies are needed for specification of physiological or pathological role of NAAb to endogenic mediators.

[Immunoenzyme technique for detection of natural antibodies to bradykinin in cardiovascular diseases]. / Immunofermentnyi metod vyiavleniia estestvennykh antitel protiv bradikinina pri serdechno-sosudistykh zabolevaniiakh.

A method for detecting natural antibodies to bradykinin by ELISA has been developed. A polymeric matrix model of peptide antigen has been synthesized for this purpose. Optimal conditions for detecting antigen and antibodies in the sera of donors and cardiovascular patients have been chosen. The level of antibodies to bradykinin is constant in donors and shifted in patients.

[The effect of an antiatherosclerotic diet including omega-3 polyunsaturated fatty acids of marine and plant origins on the indices of cellular and humoral immunity in patients with ischemic heart disease and a disordered carbohydrate tolerance]. / Vliianie antiateroskleroticheskoi diety s vkliucheniem polinenasyshchennykh zhirnykh kislot omega-3 morskogo i rastitel'nogo proiskhozhdeniia na pokazateli kletochnogo i gumoral'nogo immuniteta u bol'nykh ishemicheskoi bolezn'iu serdtsa s narushennoi tolerantnost'iu k uglevodam.

The dynamic of natural antibodies against catecholamines and alpha-2-macroglobulin, thrombin, antithrombin III and parameters of cellular immunity in 92 patients with ischemic heart disease and ischemic heart disease complicated by impaired glucose tolerance was studied of influence of antiatherosclerotic diet with fish and vegetable PUFA omega-3 from "Eicolen". Besides favorable influence to a clinical picture of the disease universal normalizing influence of antiatherosclerotic diet with addition Eicolen on parameters of humoral a cellular immunity.

[Detection of natural anti-angiotensin converting enzyme antibodies in human serum using immunoenzyme technique ]. / Opredelenie estestvennykh antitel k angiotenzinprevrashchaiushchemu frmentu v syvorotke krovi cheloveka immunofermentnym metodom.

Solid-phase enzyme immunoassay (EIA) was developed for detecting natural antibodies to angiotensine-converting enzyme (ACE). Optimal conditions for detecting natural anti-ACE by EIA in the sera of donors and patients with disorders of arterial pressure are selected. The findings indicate that the level of natural anti-ACE is normally constant, while in the patients it is increased in 50% cases.

[Immunoenzyme assay for detection of natural antibodies against vasopressin in systemic lupus erythematosus]. / Vyiavlenie estestvennykh antitel protiv vazopressina pri sistemnoi krasnoi volchanke immunofermentnym metodom.

A solid-phase enzyme immunoassay procedure for detecting natural antibodies to vasopressin (VP) is developed. For this purpose, a VP antigen is synthesized on polymer matrix. Optimal conditions of enzyme immunoassay for detecting antibodies to this antigen in the sera of donors and patients with systemic lupus erythematosus (SLE) are selected. The level of anti-VP antibodies is constant in donors and shifted in SLE patients. Changes in the level of natural antibodies to VP correlate with immunochemical parameters.

[Dynamics of the natural antibody level as affected by the biologically active food supplement "Vitol" in patients with hypertensive disease and obesity]. / Izuchenia dinamiki urovnia estestvennykh antitel pod vliianiem biologicheski aktivnoi dobavki k pishche "Vitol" u bol'nykh gipertonicheskoi bolezn'iu i ozhireniem.

The dynamics of level of natural antibodies to catecholamines (adrenaline, noradrenaline and dopamine) and factors of blood coagulation system (thrombin, antithrombin III and alpha 2-macroglobulin) in 122 patients with hypertension and obesity was studied in hyposodium antiatherosclerotic diet and this diet enriched with phospholipid preparation "Vitol" during 4 weeks. Universal normalizing effect of the diet consists in increase of levels of natural antibodies to adrenaline, noradrenaline, dopamine, thrombin, antithrombin III and alpha 2-macroglobulin was found. The influence of the diet was more expressed during it enrichment by "Vitol".