RESUMO
H(+)-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H(+)-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
Assuntos
Hemina/fisiologia , Lactococcus lactis/metabolismo , ATPases Translocadoras de Prótons/metabolismo , Membrana Celular/metabolismo , Hemina/metabolismo , Lactococcus lactis/enzimologia , Lactococcus lactis/crescimento & desenvolvimento , Mutação , ATPases Translocadoras de Prótons/genéticaRESUMO
Escherichia coli atp mutants, which lack a functional H+-ATPase complex, are capable of growth on glucose but not on succinate or other C4-dicarboxylates (Suc- phenotype). Suc+ revertants of an atp deletion strain were isolated which were capable of growth on succinate even though they lack the entire H+-ATPase complex. Complementation in trans with the yhiF gene suppressed the growth of the Suc+ mutants on succinate, which implicates the yhiF gene product in the regulation of C4-dicarboxylate metabolism. Indeed, when the E. coli C4-dicarboxylate transporter (encoded by the dctA gene) was expressed in trans, the Suc- phenotype of the atp deletion strain reverted to Suc+, which shows that the reason why the E. coli atp mutant is unable to grow aerobically on C4-dicarboxylates is insufficient transport capacity for these substrates.
Assuntos
Proteínas de Bactérias , Transportadores de Ácidos Dicarboxílicos , Escherichia coli/crescimento & desenvolvimento , ATPases Translocadoras de Prótons/metabolismo , Succinatos/metabolismo , Transporte Biológico , Proteínas de Transporte/genética , Mapeamento Cromossômico , Meios de Cultura , Escherichia coli/genética , Escherichia coli/metabolismo , Deleção de Genes , Teste de Complementação Genética , Mutagênese , Fenótipo , ATPases Translocadoras de Prótons/genética , TransposasesRESUMO
Strains carrying deletions in the atp genes, encoding the H(+)-ATPase, were unable to grow on nonfermentable substrates such as succinate, whereas with glucose as the substrate the growth rate of an atp deletion mutant was surprisingly high (some 75-80% of wild-type growth rate). The rate of glucose and oxygen consumption of these mutants was increased compared to the wild-type rates. In order to analyze the importance of the H(+)-ATPase at its physiological level, the cellular concentration of H(+)-ATPase was modulated around the wild-type level, using genetically manipulated strains. The control coefficient by the H(+)-ATPase with respect to growth rate and catabolic fluxes was measured. Control on growth rate was absent at the wild-type concentration of H(+)-ATPase, independent of whether the substrate for growth was glucose or succinate. Control by the H(+)-ATPase on the catabolic fluxes, including respiration, was negative at the wild-type H(+)-ATPase level. Moreover, the turnover number of the individual H(+)-ATPase enzymes increased as the H(+)-ATPase concentration was lowered. The negative control by the H(+)-ATPase on catabolism may thus be involved in a homeostatic control of ATP synthesis and, to some extent, explain the zero control by the H(+)-ATPase on E. coli growth rate.
Assuntos
Escherichia coli/enzimologia , Genes Bacterianos , ATPases Translocadoras de Prótons/metabolismo , Escherichia coli/genética , Escherichia coli/fisiologia , Deleção de Genes , Glucose/metabolismo , Cinética , Óperon , Consumo de Oxigênio , ATPases Translocadoras de Prótons/genética , Succinatos/metabolismoRESUMO
The H(+)-ATPase plays a central role in Escherichia coli free-energy transduction and hence in E. coli physiology. We here investigate the extent to which this enzyme also controls the growth rate, growth yield, and respiratory rate of E. coli. We modulate the expression of the atp operon and determine the effect on said properties. When quantified in terms of control coefficients, we find that, in the wild-type cell growing on glucose in minimal medium, this key enzyme (H(+)-ATPase) exerts virtually no control on growth rate (magnitude of C < 0.01), a minor positive control on growth yield (C = 0.15), and a small but negative control on respiration rate (C = -0.25). The control the enzyme exerts on the consumption rate of the carbon and free-energy substrate is negative (C = -0.15). We also studied how the control coefficients themselves vary with the expression of the atp operon. As the level of expression of the atp operon was reduced, the control exerted by the H(+)-ATPase on growth rate and growth yield increased slightly; the control on growth rate passed through a maximum (C = 0.1) and disappeared when the atp operon was not expressed at all, reflecting that with this substrate there are alternative routes for ATP synthesis. At elevated levels of the H(+)-ATPase compared to the wild type, the control exerted by the enzyme on growth rate became negative. The evolutionary context of the absence of control by the atp operon on growth rate is discussed.
Assuntos
Escherichia coli/fisiologia , ATPases Translocadoras de Prótons/metabolismo , Divisão Celular , Cromossomos Bacterianos , Escherichia coli/enzimologia , Escherichia coli/genética , Genótipo , Cinética , Substâncias Macromoleculares , Óperon , Consumo de Oxigênio , Regiões Promotoras Genéticas , ATPases Translocadoras de Prótons/genéticaRESUMO
Individual subunits of ATP synthase, encoded by the eight genes of the atp operon (atpA through atpH), have been found to be synthesized at a 10-fold range in molar amounts (D.L. Foster and R.H. Fillingame, J. Biol. Chem. 257:2009-2015, 1982; K. von Meyenburg, B.B. Jorgensen, J. Nielsen, F.G. Hansen, and O. Michelsen. Tokai J. Exp. Clin. Med. 7:23-31, 1982). We have determined the functional half-lives at 30 degrees C of mRNAs transcribed from these genes either during constitutive expression in a partial diploid strain or after induced expression from a plasmid. Accurate decay kinetics of the relative mRNA levels were determined by monitoring the rates of synthesis of the individual ATP synthase subunits by radioactive pulse labeling at different times after blocking transcription initiation with rifampin. The mRNA transcribed from the atp operon was found to be inactivated about twice as fast as the bulk mRNA in E. coli. Exceptions are the mRNA from the promoter-proximal atpB gene, which was inactivated about three times as fast as the bulk mRNA, and atpC mRNA, the inactivation rate of which was comparable to that of the bulk mRNA. These moderate differences in the kinetics of functional decay explain only a minor part of the differences in expression levels of the atp genes. We conclude, therefore, that the individual atp mRNAs must be translated with widely different efficiencies. The present analysis further revealed that mRNA degradation is sensitive to heat shock; i.e., after incubation at 39 degrees C for 5 min followed by a shift back to 30 degrees C, the decay rate of the bulk mRNA was decreased by 30%.
Assuntos
Proteínas de Escherichia coli , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Óperon , ATPases Translocadoras de Prótons/genética , RNA Bacteriano/metabolismo , RNA Mensageiro/metabolismo , Endorribonucleases/metabolismo , Escherichia coli/enzimologia , Meia-Vida , Biossíntese de Proteínas , Mapeamento por Restrição , Ribonuclease III , Transcrição GênicaRESUMO
With succinate as free-energy source, Escherichia coli generating virtually all ATP by oxidative phosphorylation might be expected heavily to tax its ATP generating capacity. To examine this the H(+)-ATPase (ATP synthase) was modulated over a 30-fold range. Decreasing the amount of H(+)-ATPase reduced the growth rate much less than proportionally; the H(+)-ATPase controlled growth rate by < 10%. This lack of control reflected excess capacity: the rate of ATP synthesis per H(+)-ATPase (the turnover number) increased by 60% when the number of enzymes was decreased by 40%. At 15% H(+)-ATPase, the enzyme became limiting and its turnover was increased even further, due to an increased driving force caused by a reduction in the total flux through the enzymes. At smaller reductions of [H(+)-ATPase] the total flux was not reduced, revealing a second cause for increased turnover number through increased membrane potential: respiration was increased, showing that in E.coli, respiration and ATP synthesis are, in part, inversely coupled. Indeed, growth yield per O2 decreased, suggesting significant leakage or slip at the high respiration rates and membrane potential found at low H(+)-ATPase concentrations, and explaining that growth yield may be increased by activating the H(+)-ATPase.
Assuntos
Escherichia coli/metabolismo , ATPases Translocadoras de Prótons/metabolismo , Nucleotídeos de Adenina/metabolismo , Proteínas de Bactérias/metabolismo , Metabolismo Energético , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Consumo de Oxigênio , ATPases Translocadoras de Prótons/genéticaRESUMO
For control analysis, it is necessary to modulate the activity of an enzyme around its normal level and measure the changes in steady-state fluxes or concentrations. We describe an improved method for effecting the modulation, as elaborated for Escherichia coli. The chromosomal gene, encoding the enzyme of interest, is put under the control of a lacUV5 or a tacI promoter. The alternative use of the two promoters leads to an expression range which should make it suitable for the use in control analysis of many enzymes. The lacUV5 promoter should be used when the wild-type expression level is low, the tacI promoter when the latter is high. The endogenous lac operon is placed under the control of a second copy of the lacUV5 promoter and a lacY7am mutation (eliminating lactose permease, the transport system for the inducer isopropyl-thio-beta-D- galactoside) is introduced. The method was demonstrated experimentally by constructing E. coli strains, in which the chromosomal atp operon is transcribed from the lacUV5 and the tacI promoter. We measured the concentration of the c subunit of H(+)-ATPase, and found that the expression of this enzyme could be modulated between non-detectable levels and up to five times the wild-type level. Thus, in the absence of inducer, no expression of atp genes could be detected when the atp operon was controlled by the lacUV5 promoter, and we estimate that the expression was less than 0.0025 times the wild-type level. We show that the introduction of a lacY mutation facilitated the attainment of steady induction levels of partially induced cells. The mutation also reduced positive cooperativity in the dependence of expression on the concentration of isopropyl-thio-beta-D-galactoside (the inducer) and shifted the concentration of inducer needed for half maximum induction to higher values. These properties should facilitate the experimental modulation of the enzyme activity by varying the concentration of the inducer.
Assuntos
Proteínas de Escherichia coli , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Óperon Lac , Proteínas de Transporte de Monossacarídeos , Regiões Promotoras Genéticas , Simportadores , Clonagem Molecular , Genes Bacterianos , Engenharia Genética , Proteínas de Membrana Transportadoras/genética , Óperon , PlasmídeosRESUMO
The membrane-bound H(+)-ATPase plays a key role in free-energy transduction of biological systems. We report how the carbon and energy metabolism of Escherichia coli changes in response to deletion of the atp operon that encodes this enzyme. Compared with the isogenic wild-type strain, the growth rate and growth yield were decreased less than expected for a shift from oxidative phosphorylation to glycolysis alone as a source of ATP. Moreover, the respiration rate of a atp deletion strain was increased by 40% compared with the wild-type strain. This result is surprising, since the atp deletion strain is not able to utilize the resulting proton motive force for ATP synthesis. Indeed, the ratio of ATP concentration to ADP concentration was decreased from 19 in the wild type to 7 in the atp mutant, and the membrane potential of the atp deletion strain was increased by 20%, confirming that the respiration rate was not controlled by the magnitude of the opposing membrane potential. The level of type b cytochromes in the mutant cells was 80% higher than the level in the wild-type cells, suggesting that the increased respiration was caused by an increase in the expression of the respiratory genes. The atp deletion strain produced twice as much by-product (acetate) and exhibited increased flow through the tricarboxylic acid cycle and the glycolytic pathway. These three changes all lead to an increase in substrate level phosphorylation; the first two changes also lead to increased production of reducing equivalents. We interpret these data as indicating that E. coli makes use of its ability to respire even if it cannot directly couple this ability to ATP synthesis; by respiring away excess reducing equivalents E. coli enhances substrate level ATP synthesis.
Assuntos
Carbono/metabolismo , Metabolismo Energético , Escherichia coli/metabolismo , ATPases Translocadoras de Prótons/metabolismo , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Ciclo do Ácido Cítrico , Grupo dos Citocromos b , Escherichia coli/genética , Deleção de Genes , Glicólise , Potenciais da Membrana , Oxirredução , Consumo de Oxigênio , ATPases Translocadoras de Prótons/genéticaRESUMO
The uncoupler resistant bacterial strains E. coli Tuv and Cuv share the high deoxycholate sensitivity of the parent strain, Doc S. However, both Tuv and Cuv show greater resistance than Doc S to other detergents. Measurement of the periplasmic volume indicates that the outer membrane of Doc S is freely permeable to both TPP+ and hydroxymethylinulin. Tuv and Cuv are able to exclude these compounds. EDTA treatment was necessary prior to measuring membrane potential in Tuv and Cuv. Under conditions where delta phi could be measured, uncouplers acted to dissipate delta phi with equal potency in all strains. Uncoupler resistant proline uptake in Tuv and Cuv was abolished by EDTA treatment. Transduction experiments with phage P1 showed that uncoupler resistance could be transferred from Tuv to Doc S. Such transductants were no longer sensitive to novabiocin. The gene for uncoupler resistance cotransduced with the gene pyrE (82 min). Plating efficiency experiments with P1 suggests that detergent sensitivity in Doc S arises from an rfa (81 min) mutation. This mutation is no longer present in Tuv.
Assuntos
Benzimidazóis/farmacologia , Ácido Desoxicólico/farmacologia , Escherichia coli/fisiologia , Desacopladores/farmacologia , Membrana Celular/efeitos dos fármacos , Membrana Celular/fisiologia , Permeabilidade da Membrana Celular , Citoplasma/fisiologia , Ácido Edético/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Ácidos Graxos/isolamento & purificação , Indicadores e Reagentes , Lipídeos de Membrana/isolamento & purificação , Oniocompostos , Compostos OrganofosforadosRESUMO
The atpB encodes the a [corrected] subunit of the H(+)-ATPase of E. coli. The topology of this membrane protein has been analyzed by PhoA fusions. The results support an eight transmembrane segment model that is consistent with the hydropathic profile.
Assuntos
Clonagem Molecular , Escherichia coli/enzimologia , Regulação Enzimológica da Expressão Gênica , Proteínas de Membrana/análise , ATPases Translocadoras de Prótons/análise , Fosfatase Alcalina/análise , Citoplasma/análise , Escherichia coli/genética , Genes Letais , Proteínas de Membrana/genética , Modelos Químicos , Peptídeos/análise , Plasmídeos , Conformação Proteica , ATPases Translocadoras de Prótons/genética , Proteínas Recombinantes de Fusão/análiseRESUMO
The amount and type of dietary protein affect bone mineral loss after the menopause. This observation was substantiated in 10 y of studies by direct photon absorptiometry, four results of which follow. 1) Studies of 1600 women in southwestern Michigan revealed that those who had followed the lactoovovegetarian diet for at least 20 y had only 18% less bone mineral by age 80 whereas closely paired omnivores had 35% less bone mineral. 2) A study of self-selected weighed food intake showed no statistical difference in nutrient intakes but a difference in Ca:P ratio and acid-base formation of diet, each significant to p less than 0.001. 3) When sulfur intake of a fixed diet was increased, the titratable acidity of the urine increased proportionately. 4) Bone mineral densities of 304 older women from the continental United States closely paralleled those from earlier Michigan studies.
Assuntos
Osso e Ossos/análise , Dieta Vegetariana , Estilo de Vida , Minerais/análise , Adulto , Idoso , Dieta , Ingestão de Energia , Feminino , Humanos , Pessoa de Meia-Idade , Osteoporose/epidemiologia , Estados UnidosRESUMO
Transcriptional fusions between the phage lambda promotor pR and ATP synthase genes, atp, on plasmid pBR322 were constructed in order to study the effects upon growth and physiology of Escherichia coli of induced overproduction of H+-ATPase subunits. Constitutive overproduction of the complete enzyme had earlier been found to result in decreased growth rate and cytological defects. When a 15-fold overproduction of subunit a alone, or together with subunit c, or with all other ATP synthase subunits was suddenly induced, the following effects were observed. Inhibition of growth and protein synthesis within 10 min of induction, which effect was suppressed by N,N'-dicyclohexylcarbodiimide, also when the chromosomal atp genes coding for the Fo subunits a, b and c were deleted. Partial collapse of the membrane potential delta psi at 4-6 min after induction paralleled by inhibition of thiomethylgalactoside and guanosine transport. Respiration and alpha-methylglucoside transport was not affected. The partial collapse of delta psi, and the specific inhibition of proton-driven transport systems is taken to show that the subunit a has--when suddenly overproduced and inserted into the membrane--a protonophoric activity. It is suggested that this protonophoric activity of subunit a is related to the function of this subunit in the Fo sector in H+-ATPases.
Assuntos
Escherichia coli/enzimologia , Genes Bacterianos , Genes , ATPases Translocadoras de Prótons/metabolismo , Bacteriófago lambda/genética , Membrana Celular/enzimologia , Enzimas de Restrição do DNA , Escherichia coli/genética , Genótipo , Cinética , Substâncias Macromoleculares , Plasmídeos , ATPases Translocadoras de Prótons/genética , Transcrição GênicaRESUMO
Integration into the cytoplasmic membrane and function of the three F0 subunits, a, b and c, of the membrane-bound ATP synthase of Escherichia coli K12 were analysed in situations where synthesis of only one or two types of subunits was possible. This was achieved by combined use of atp mutations and plasmids carrying and expressing one or two of the atp genes coding for ATP synthase subunits. AU three F0 subunits were found to be required for the establishment of efficient H+ conduction. Subunits a and b individually as well as together were found to bind F1 ATPase to the membrane while subunit c did not. The ATPase activity bound to either of these single subunits, or in pairwise combinations, was not inhibited by N,N'-dicyclohexylcarbodiimide. Also ATP-dependent H+ translocation was not catalysed unless all three F0 subunits were present in the membrane. The integration into the membrane of the subunits a and b was independent of the presence of other ATP synthase subunits.
Assuntos
ATPases Bacterianas Próton-Translocadoras/metabolismo , ATPases Bacterianas Próton-Translocadoras/genética , Membrana Celular/metabolismo , Permeabilidade da Membrana Celular , Escherichia coli/enzimologia , Escherichia coli/genética , Mutagênese , PrótonsRESUMO
The structure of the atp operon, which contains the genes for the eight subunits alpha, beta, gamma, delta, epsilon, a, b and c of the membrane bound ATP synthase of Escherichia coli as determined by genetic experiments and DNA sequencing, is reviewed. The localization of transcription signals, namely of one major and two minor promoters, as well as the determination of the stoichiometry of the subunits (alpha:beta:gamma:delta:epsilon:a:b:c = 3:3:1:1:1:1:2:12-15) is summarized.
Assuntos
Escherichia coli/enzimologia , Genes Bacterianos , Genes , Complexos Multienzimáticos/genética , Óperon , Fosfotransferases/genética , Complexos de ATP Sintetase , Sequência de Bases , Membrana Celular/enzimologia , Clonagem Molecular , Enzimas de Restrição do DNA , Escherichia coli/genética , Substâncias Macromoleculares , Peso Molecular , Mutação , Transcrição GênicaRESUMO
The ribonucleoside triphosphate, deoxyribonucleoside triphosphate, 3' -diphosphate guanosine 5' -diphosphate (ppGpp), and 5-phosphoribosyl 1-pyrophosphate (PRPP) pools in Escherichia coli B were determined by thin-layer chromatography during changing conditions to ammonium starvation. The intracellular concentrations of all nucleotides were found to change in a well-defined order several minutes before andy observed change in the optical density of the culture. The levels of purine nucleoside triphosphates (adenosine 5' -triphosphate [CTP], dCTP) and uridine nucleotides (uridine 5' -triphosphate, deoxythymidine 5'-triphosphate). The deoxyribonucleotides thus behaved as the ribonucleotides. The levels of ppGpp increased 11-fold after the decrease in uridine nucleotides, when the accumulation of stable ribonucleic acid (RNA) stopped. The level of the nucleotide pool did not stabilize until 30 min after the change in optical density. The pool of dGTP dropped concomitantly with the pool of CTP. The nucleotide precursor PRPP exhibited a transient increase, wtih maximum value of four times the exponential levels at the onset of starvation. Apparently the cell adjusts early to starvation by reducing either the phosphorylating activity or the nucleotide biosynthetic activity. As in other downshift systems, the accumulation of stable RNA stopped before the break in optical density and before the stop in protein accumulation. Cell divisions were quite insensitive to the control mechanisms operating on RNA and protein accumulation under ammonium starvation, since the cells continued to divide for 21 min without any net accumulation of RNA.
Assuntos
Escherichia coli/metabolismo , Nitrogênio/metabolismo , Nucleotídeos/metabolismo , Pentosefosfatos/metabolismo , Fosforribosil Pirofosfato/metabolismo , Trifosfato de Adenosina/metabolismo , Proteínas de Bactérias/metabolismo , Nucleotídeos de Citosina/metabolismo , Guanosina Trifosfato/metabolismo , RNA Bacteriano/metabolismo , Nucleotídeos de Timina/metabolismo , Nucleotídeos de Uracila/metabolismoRESUMO
Neutron activation followed by gammagamma-coincidence measurements of 9-3 hr (152)Eu is shown to be a convenient method for routine determination of europium in minerals and rocks. In the concentration range 10-150 ppm the method is free of interference from other elements, and neutron shielding effects do not interfere seriously in the analysis. The precision of the method is about 5%.
RESUMO
The copper content of some geological samples has been determined by thermal neutron activation and subsequent coincidence gamma-spectrometry, taking advantage of the 180 degrees annihilation quanta of (64)Cu. By this means the interference of (24)Na, which is often the major gamma-activity induced in geological materials, is greatly reduced. The method is precise to about +/- 5 %, and the error is of the same order. The method should be especially attractive for application to samples with a copper content of 100-1000 ppm.