RESUMO
Muscle biopsies of the mandible adductors of the Wistar rat (Rattus norvegicus Berkenhout) have been analyzed enzyme-histochemically for the investigation of the postnatal development (42.-126. d post partum) of their muscle fibers with special regards to the fiber types. The following methods have been used in this investigation: myofibrillar adenosine triphosphatase (ATP-ase) with different pre-incubations, Sudan black B (triglycerides), periodic acid-Schiff reagent (PAS) (glycogen), Gomori modified Trichrome staining. Quantitative analysis of muscle fibre-type composition and muscle fibre size was done from different regions of muscle sections. Estimation of the fiber size was carried out by measuring the minimum diameter of each type of muscle fibre.
Assuntos
Músculos da Mastigação/crescimento & desenvolvimento , Desenvolvimento Muscular , Ratos Endogâmicos/crescimento & desenvolvimento , Adenosina Trifosfatases/análise , Animais , Glicogênio/análise , Histocitoquímica , Músculo Masseter/análise , Músculo Masseter/crescimento & desenvolvimento , Músculos da Mastigação/análise , Reação do Ácido Periódico de Schiff , Músculos Pterigoides/análise , Músculos Pterigoides/crescimento & desenvolvimento , Ratos , Músculo Temporal/análise , Músculo Temporal/crescimento & desenvolvimento , Triglicerídeos/análiseRESUMO
224 muscle biopsies of the mandible adductors of the Wistar rat have been analyzed enzyme-histochemically for the investigation of their muscle fibre types. The myofibrillar adenosine triphosphatase (ATPase) and the succinic dehydrogenase (SDH) have been used in this investigation. The major part of the chewing musculature consists of muscle fibres of the type II, which can be subdivided into 3 subtypes, type IIA, IIB and TR.
