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1.
Sci Rep ; 14(1): 17845, 2024 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-39090173

RESUMO

The core of clinic treatment of Parkinson's disease (PD) is to enhance dopamine (DA) signaling within the brain. The regulation of dopamine transporter (DAT) is integral to this process. This study aims to explore the regulatory mechanism of glial cell line-derived neurotrophic factor (GDNF) on DAT, thereby gaining a profound understanding its potential value in treating PD. In this study, we investigated the effects of GDNF on both cellular and mouse models of PD, including the glycosylation and membrane transport of DAT detected by immunofluorescence and immunoblotting, DA signal measured by neurotransmitter fiber imaging technology, Golgi morphology observed by electron microscopic, as well as cognitive ability assessed by behavior tests. This study revealed that in animal trials, MPTP-induced Parkinson's Disease (PD) mice exhibited a marked decline in cognitive function. Utilizing ELISA and neurotransmitter fiber imaging techniques, we observed a decrease in dopamine levels and a significant reduction in the intensity of dopamine signal release in the Prefrontal Cortex (PFC) of PD mice induced by MPTP. Intriguingly, these alterations were reversed by Glial Cell Line-Derived Neurotrophic Factor (GDNF). In cellular experiments, following MPP + intervention, there was a decrease in Gly-DAT modification in both the cell membrane and cytoplasm, coupled with an increase in Nongly-DAT expression and aggregation of DAT within the cytoplasm. Conversely, GDNF augmented DAT glycosylation and facilitated its membrane transport in damaged dopaminergic neurons, concurrently reversing the effects of GRASP65 depletion and Golgi fragmentation, thereby reducing the accumulation of DAT in the Golgi apparatus. Furthermore, overexpression of GRASP65 enhanced DAT transport in PD cells and mice, while suppression of GRASP65 attenuated the efficacy of GDNF on DAT. Additionally, GDNF potentiated the reutilization of neurotransmitters by the PFC presynaptic membrane, boosting the effective release of dopamine following a single electrical stimulation, ultimately ameliorating the cognitive impairments in PD mice.Therefore, we propose that GDNF enhances the glycosylation and membrane trafficking of DAT by facilitating the re-aggregation of the Golgi apparatus, thereby amplifying the utilization of DA signals. This ultimately leads to the improvement of cognitive abilities in PD mouse models. Our study illuminates, from a novel angle, the beneficial role of GDNF in augmenting DA utilization and cognitive function in PD, providing fresh insights into its therapeutic potential.


Assuntos
Cognição , Proteínas da Membrana Plasmática de Transporte de Dopamina , Dopamina , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Animais , Fator Neurotrófico Derivado de Linhagem de Célula Glial/metabolismo , Glicosilação , Proteínas da Membrana Plasmática de Transporte de Dopamina/metabolismo , Camundongos , Cognição/efeitos dos fármacos , Dopamina/metabolismo , Masculino , Doença de Parkinson/metabolismo , Modelos Animais de Doenças , Camundongos Endogâmicos C57BL , Membrana Celular/metabolismo , Córtex Pré-Frontal/metabolismo
2.
Biol Trace Elem Res ; 126(1-3): 257-68, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18709336

RESUMO

Seedlings of spinach were grown in Hoagland's medium containing 0, 20, 40, 60, 80, 100 microM PbCl2, respectively, for 4 weeks. Chloroplasts were assayed for overproduction of reactive oxygen species (ROS) such as superoxide radicals (O2(*-)) and hydrogen peoxide (H2O2) and of lipid peroxide (malonyldialdehyde) and for activities of the antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase and glutathione content, oxygen-evolving rate, and chlorophyll content. Increase in both ROS and lipid peroxide content and reduction in photosynthesis and activities of the antioxidant defense system indicated that spinach chloroplast underwent a stress condition due to an oxidative attack. Seedling growth cultivated in containing Pb2+ media was significantly inhibited. The results imply that spinach chloroplast was not able to tolerate the oxidative stress induced by Pb2+ due to having no effective antioxidant defense mechanism.


Assuntos
Cloroplastos/efeitos dos fármacos , Cloroplastos/metabolismo , Chumbo/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Spinacia oleracea/efeitos dos fármacos , Spinacia oleracea/metabolismo , Antioxidantes/metabolismo , Ascorbato Peroxidases , Catalase/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidase/metabolismo , Peroxidases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
3.
Biol Trace Elem Res ; 121(1): 69-79, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18186002

RESUMO

A proven photocatalyst, titanium dioxide in the form of nano-anatase, is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase could absorb ultraviolet light (UV-B) and convert light energy to stable chemistry energy finally via electron transport in spinach chloroplasts. The mechanisms by which nano-anatase promotes antioxidant stress in spinach chloroplasts under UV-B radiation are still not clearly understood. In the present paper, we investigate the effects of nano-anatase on the antioxidant stress in spinach chloroplasts under UV-B radiation. The results showed that nano-anatase treatment could significantly decrease accumulation of superoxide radicals O2.-, hydrogen peroxide (H2O2), and malonyldialdehyde (MDA) content, and increase activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and elevate evolution oxygen rate in spinach chloroplasts under UV-B radiation. Together, nano-anatase could decrease the oxidative stress to spinach chloroplast caused by UV-B radiation.


Assuntos
Antioxidantes/metabolismo , Cloroplastos/efeitos dos fármacos , Cloroplastos/efeitos da radiação , Titânio/farmacologia , Raios Ultravioleta , Ascorbato Peroxidases , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Peroxidases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Spinacia oleracea
4.
Biol Trace Elem Res ; 122(1): 73-81, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17962910

RESUMO

Linolenic acid is an inhibitor of electron transport in chloroplasts of higher plants. It has obvious effects on the structure and function of chloroplasts. In the present paper, we investigated the nano-anatase relieving the inhibition of photoreduction activity and oxygen evolution caused by linolenic acid in spinach chloroplasts. The results showed that linolenic acid in various concentrations could obviously reduce the whole chain electron transport and the photoreduction activity of two photosystems, especially on the oxidative reside and reduce reside of photosystem II (PS II). After adding nano-anatase to chloroplasts treated by linolenic acid, the whole chain electron transport rate, the photoreduction activity of two photosystems, and the oxygen evolution rate were increased significantly, indicating that nano-anatase could obviously decrease the inhibition of linolenic acid on the electron transport, photoreduction activity, and oxygen evolution of spinach chloroplasts.


Assuntos
Cloroplastos/metabolismo , Transporte de Elétrons/efeitos dos fármacos , Complexo de Proteína do Fotossistema I/metabolismo , Complexo de Proteína do Fotossistema II/metabolismo , Titânio/metabolismo , Ácido alfa-Linolênico/metabolismo , Fotoquímica , Spinacia oleracea/metabolismo
5.
Biol Trace Elem Res ; 121(3): 258-65, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17955201

RESUMO

Lead (Pb(2+)) is a well-known highly toxic element. The mechanisms of the Pb(2+) toxicity are not well understood for nitrogen metabolism of higher plants. In this paper, we studied the effects of various concentrations of PbCl(2) on the nitrogen metabolism of growing spinach. The experimental results showed that Pb(2+) treatments significantly decreased the nitrate nitrogen (NO(-)(3)-N) absorption and inhibited the activities of nitrate reductase, glutamate dehydrogenase, glutamine synthase, and glutamic-pyruvic transaminase of spinach, and inhibited the synthesis of organic nitrogen compounds such as protein and chlorophyll. However, Pb(2+) treatments increased the accumulation of ammonium nitrogen NH(+)(4)-N)in spinach cell. It implied that Pb(2+) could inhibit inorganic nitrogen to be translated into organic nitrogen in spinach, thus led to the reduction in spinach growth.


Assuntos
Chumbo/farmacologia , Nitrogênio/metabolismo , Spinacia oleracea/efeitos dos fármacos , Alanina Transaminase/antagonistas & inibidores , Alanina Transaminase/metabolismo , Glutamato Desidrogenase/antagonistas & inibidores , Glutamato Desidrogenase/metabolismo , Glutamato-Amônia Ligase/antagonistas & inibidores , Glutamato-Amônia Ligase/metabolismo , Nitrato Redutase/antagonistas & inibidores , Nitrato Redutase/metabolismo , Nitratos/metabolismo , Compostos de Amônio Quaternário/metabolismo , Spinacia oleracea/enzimologia , Spinacia oleracea/metabolismo
6.
Biol Trace Elem Res ; 119(2): 183-92, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17916941

RESUMO

Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. The mechanisms by which nano-anatase promotes energy transfer and the conversion efficiency of the process are still not clearly understood. In the present paper, we report the results obtained with the photosystem II (PSII) isolated from spinach and treated by nano-anatase TiO2 and studied the effect of nano-anatase TiO2 on energy transfer in PSII by spectroscopy and on oxygen evolution. The results showed that nano-anatase TiO2 treatment at a suitable concentration could significantly change PSII microenvironment and increase absorbance for visible light, improve energy transfer among amino acids within PSII protein complex, and accelerate energy transport from tyrosine residue to chlorophyll a. The photochemical activity of PSII (fluorescence quantum yield) and its oxygen-evolving rate were enhanced by nano-anatase TiO2. This is viewed as evidence that nano-anatase TiO2 can promote energy transfer and oxygen evolution in PSII of spinach.


Assuntos
Transferência de Energia/efeitos dos fármacos , Oxigênio/metabolismo , Complexo de Proteína do Fotossistema II/efeitos dos fármacos , Complexo de Proteína do Fotossistema II/metabolismo , Titânio/farmacologia , Clorofila/metabolismo , Clorofila A , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Spinacia oleracea
7.
Biol Trace Elem Res ; 120(1-3): 273-83, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17916980

RESUMO

In the article, we report that effects of nano-anatase on the spectral characteristics and content of light-harvesting complex II (LHCII) on the thylakoid membranes of spinach were investigated. The results showed that nano-anatase treatment could increase LHCII content on the thylakoid membranes of spinach and the trimer of LHCII; nano-anatase could enter the spinach chloroplasts and bind to PSII. Meanwhile, spectroscopy assays indicated that the absorption intensity of LHCII from nano-anatase-treated spinach was obviously increased in the red and the blue region, fluorescence quantum yield near 685 nm of LHCII was enhanced, the fluorescence excitation intensity near 440 and 480 nm of LHCII significantly rose and F 480/F 440 ratio was reduced. Oxygen evolution rate of PSII was greatly improved. Together, nano-anatase promoted energy transferring from chlorophyll (chl) b and carotenoid to chl a, and nano-anatase TiO2 was photosensitized by chl of LHCII, which led to enhance the efficiency of absorbing, transferring, and converting light energy.


Assuntos
Complexos de Proteínas Captadores de Luz/metabolismo , Complexo de Proteína do Fotossistema II/metabolismo , Tilacoides/efeitos dos fármacos , Titânio/farmacologia , Cloroplastos/metabolismo , Complexos de Proteínas Captadores de Luz/química , Oxigênio/metabolismo , Complexo de Proteína do Fotossistema II/química , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Spinacia oleracea , Tilacoides/química , Titânio/metabolismo
8.
Biol Trace Elem Res ; 119(1): 68-76, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17914221

RESUMO

With a photocatalyzed characteristic, nanoanatase TiO2 under light could cause an oxidation-reduction reaction. Our studies had proved that nano-TiO2 could promote photosynthesis and greatly improve spinach growth. However, the mechanism of nano-TiO2 on promoting conversion from light energy to electron energy and from electron energy to active chemistry energy remains largely unclear. In this study, we report that the electron transfer, oxygen evolution, and photophosphorylation of chloroplast (Chl) from nanoanatase-TiO2-treated spinach were greatly increased under visible light and ultraviolet light illumination. It was demonstrated that nanoanatase TiO2 could greatly improve whole chain electron transport, photoreduction activity of photosystem II, O2-evolving and photophosphorylation activity of spinach Chl not only under visible light, but also energy-enriched electron from nanoanatase TiO2, which entered Chl under ultraviolet light and was transferred in photosynthetic electron transport chain and made NADP+ be reduced into NADPH, and coupled to photophosphorylation and made electron energy be transformed to ATP. Moreover, nanoanatase h+, which photogenerated electron holes, captured an electron from water, which accelerated water photolysis and O2 evolution.


Assuntos
Cloroplastos/metabolismo , Fotossíntese/efeitos dos fármacos , Titânio/farmacologia , 2,6-Dicloroindofenol/química , Cloroplastos/efeitos dos fármacos , Cloroplastos/efeitos da radiação , Transporte de Elétrons/efeitos dos fármacos , Luz , Nanocompostos , Fotoquímica , Fotofosforilação/efeitos dos fármacos , Spinacia oleracea/citologia , Spinacia oleracea/efeitos dos fármacos , Spinacia oleracea/efeitos da radiação , Raios Ultravioleta
9.
Biol Trace Elem Res ; 118(2): 120-30, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17873354

RESUMO

The effects of nano-anatase TiO2 on light absorption, distribution, and conversion, and photoreduction activities of spinach chloroplast were studied by spectroscopy. Several effects of nano-anatase TiO2 were observed: (1) the absorption peak intensity of the chloroplast was obviously increased in red and blue region, the ratio of the Soret band and Q band was higher than that of the control; (2) the great enhancement of fluorescence quantum yield near 680 nm of the chloroplast was observed, the quantum yield under excitation wavelength of 480 nm was higher than the excitation wavelength of 440 nm; (3) the excitation peak intensity near 440 and 480 nm of the chloroplast significantly rose under emission wavelength of 680 nm, and F 480 / F 440 ratio was reduced; (4) when emission wavelength was at 720 nm, the excitation peaks near 650 and 680 nm were obviously raised, and F 650 / F 680 ratio rose; (5) the rate of whole chain electron transport, photochemical activities of PSII DCPIP photoreduction and oxygen evolution were greatly improved, but the photoreduction activities of PSI were a little changed. Together, the studies of the experiments showed that nano-anatase TiO2 could increase absorption of light on spinach chloroplast and promote excitation energy to be absorbed by LHCII and transferred to PSII and improve excitation energy from PSI to be transferred to PSII, thus, promote the conversion from light energy to electron energy and accelerate electron transport, water photolysis, and oxygen evolution.


Assuntos
Cloroplastos/metabolismo , Membranas Intracelulares/metabolismo , Luz , Oxirredução , Fármacos Fotossensibilizantes , Spinacia oleracea , Titânio , Cloroplastos/química , Transporte de Elétrons/fisiologia , Membranas Intracelulares/química , Oxigênio/metabolismo , Fotoquímica , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/metabolismo , Espectrometria de Fluorescência , Spinacia oleracea/citologia , Spinacia oleracea/metabolismo , Titânio/química , Titânio/metabolismo
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