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Contextualização: Diante da pandemia COVID-19, declarada em março de 2020, a Universidade Federal de Minas Gerais se adaptou aos desafios da telessaúde para o estágio supervisionado em Terapia Ocupacional, na saúde da criança e adolescente, respaldada pela resolução no 516 do Conselho Federal de Fisioterapia e Terapia Ocupacional. Processo de Intervenção/acompanhamento: Este trabalho relata a criação do serviço remoto de Terapia Ocupacional, descrevendo seu processo organizacional, coordenado para assistir 16 famílias de crianças com transtornos do desenvolvimento. Análise crítica da prática: As metas funcionais das crianças de nove das famílias foram alcançadas totalmente e 85,7% responderam positivamente, evidenciando a eficácia do modelo remoto. No entanto, observou-se dilatação da carga horária do estágio, devido à intensificação das atividades assíncronas para elaboração de materiais personalizados, aumentando a percepção de sobrecarga da equipe. Síntese das considerações: Para manter resultados funcionais positivos, evitando a sobrecarga, faz-se necessário reavaliar a carga horária destinada às atividades assíncronas.
Contextualization: Faced with the COVID-19 pandemic declared in March 2020, the Federal University of Minas Gerais adapted to the challenges of telehealth for the supervised internship in Occupational Therapy, in child and adolescent health, supported by Resolution No. 516 of the Federal Council of Physiotherapy and Occupational Therapy. Intervention process/accompaniment: The present work reports the creation of the remote Occupational Therapy service describing its coordinated organizational process to assist 16 families of children with developmental disorders. Critical analysis of the practice: The functional goals of the children of nine of the families were fully achieved and 85.7% responded positively, evidencing the effectiveness of the remote model. However, there was an increase in the workload of the internship, due to the intensification of asynchronous activities for the development of personalized materials, increasing the perception of the team's overload. Summary of considerations: To maintain positive functional results, avoiding overload, it is necessary to reassess the workload allocated to asynchronous activities.
Contextualización: Ante la pandemia COVID-19 declarada en marzo de 2020, la Universidad Federal de Minas Gerais se adaptó a los desafíos de la telesalud para la pasantía supervisada en Terapia Ocupacional, en salud infantil y adolescente, respaldada por la Resolución No. 516 del Consejo Federal. de Fisioterapia y Terapia Ocupacional. Proceso de intervención: El presente trabajo reporta la creación del servicio de Terapia Ocupacional a distancia describiendo su proceso organizativo coordinado para asistir a 16 familias de niños con trastornos del desarrollo. Análisis crítico de la práctica: Las metas funcionales de los hijos de nueve de las familias se cumplieron en su totalidad y el 85,7% respondió positivamente, evidenciando la efectividad del modelo remoto. Sin embargo, hubo un aumento en la carga de trabajo de la pasantía, debido a la intensificación de actividades asincrónicas para el desarrollo de materiales personalizados, aumentando la percepción de sobrecarga del equipo. Resumen de consideraciones: Para mantener resultados funcionales positivos, evitando la sobrecarga, es necesario reevaluar la carga de trabajo asignada a las actividades assincrônicas.
Assuntos
CriançaRESUMO
Voltage-gated sodium channels (Nav) are membrane proteins essential to initiating and propagating action potential in neurons and other excitable cells. For a given organism there are often multiple, specialized sodium channels found in different tissues, whose mutations can cause deleterious effects observed in numerous diseases. Consequently, there is high medical and pharmacological interest in these proteins. Scientific literature often uses membrane diagrams to depict important patterns in these channels including the six transmembrane segments (S1-S6) present in four different homologous domains (D1-D4), the S4 voltage sensors, the pore-lining residue segments and the ion selectivity filter residues, glycosylation and phosphorylation residues, toxin binding sites and the inactivation loop, among others. Most of these diagrams are illustrated either digitally or by hand and programs specifically dedicated to the interactive and data-friendly generation of such visualizations are scarce or non-existing. This paper describes Naview, an open-source javascript visualization compatible with modern web browsers for the dynamic drawing and annotation of voltage-gated sodium channels membrane diagrams based on the D3.js library. By using a graphical user interface and combining user-defined annotations with optional UniProt code as inputs, Naview allows the creation and customization of membrane diagrams. In this interface, a user can also map and display important sodium channel properties, residues, regions and their relationships through symbols, colors, and edge connections. Such features can facilitate data exploration and provide fast, high-quality publication-ready graphics for this highly active area of research.
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BACKGROUND: Components of the antioxidant defense system in Trypanosoma cruzi are potential targets for new drug development. Superoxide dismutases (SODs) constitute key components of antioxidant defense systems, removing excess superoxide anions by converting them into oxygen and hydrogen peroxide. The main goal of the present study was to investigate the genes coding for iron superoxide dismutase (FeSOD) in T. cruzi strains from an evolutionary perspective. METHODS: In this study, molecular biology methods and phylogenetic studies were combined with drug assays. The FeSOD-A and FeSOD-B genes of 35 T. cruzi strains, belonging to six discrete typing units (Tcl-TcVI), from different hosts and geographical regions were amplified by PCR and sequenced using the Sanger method. Evolutionary trees were reconstructed based on Bayesian inference and maximum likelihood methods. Drugs that potentially interacted with T. cruzi FeSODs were identified and tested against the parasites. RESULTS: Our results suggest that T. cruzi FeSOD types are members of distinct families. Gene copies of FeSOD-A (n = 2), FeSOD-B (n = 4) and FeSOD-C (n = 4) were identified in the genome of the T. cruzi reference clone CL Brener. Phylogenetic inference supported the presence of two functional variants of each FeSOD type across the T. cruzi strains. Phylogenetic trees revealed a monophyletic group of FeSOD genes of T. cruzi TcIV strains in both distinct genes. Altogether, our results support the hypothesis that gene duplication followed by divergence shaped the evolution of T. cruzi FeSODs. Two drugs, mangafodipir and polaprezinc, that potentially interact with T. cruzi FeSODs were identified and tested in vitro against amastigotes and trypomastigotes: mangafodipir had a low trypanocidal effect and polaprezinc was inactive. CONCLUSIONS: Our study contributes to a better understanding of the molecular biodiversity of T. cruzi FeSODs. Herein we provide a successful approach to the study of gene/protein families as potential drug targets.
