Immunohistochemical study on pigmented nevi (preliminary communication).

The human ABH blood-group antigen expression of cytokeratin, S-100 protein, and the melanoma-associated antigen was studied in 12 cases of pigmented nevi. The biotin-streptavidin immunostaining system was applied. ABH antigens were found both in the endothelial cells and in the germinative epidermal layer. The flat overlaying epithelium, single melanocytes and melanoblasts were positive for cytokeratin. Some basilar cells and the giant multinucleate cells in the Spitz nevi stained for S-100 protein. The melanoma-associated antigen was visualized in the upper epidermal layers as well as in some melanocytes. The present article discusses the specificity of the antigens studied as well as some immunologic traits of potential malignant development of Spitz nevi.

[Single-cell bacterial protein in the mixed feeds for early weaned piglets]. / Ednokletuchen bakterialen protein v kombinirani furazhi za rano otbiti praseta.

An experiment was carried out with a total of 21 hybrid Camborough pigs divided into three groups--group I, controls; group II, with 4 per cent of single-cell protein in the feed; and group III, with 8 per cent of such protein in the feed. The rations were balanced in terms of the basic feedstuffs. The pigs were kept under observation up to the age of 90 days. Three animals were periodically killed each time for investigation. Studied was the content of the total thiamine in the liver and the incorporation of the labelled 35s-thiamine in the jejunum. A histologic examination was also carried out of the jejunal mucosa and the liver. It was established that the inclusion of single-cell bacterial protein into the ration produced a positive effect on the total thiamine content of the liver. The group that was offered 8 per cent of this protein source showed reduced level of 35s-thiamine incorporation in the jejunum. Histologically, there were shortening and thickening of the intestinal villi along with signs of protein dystrophy in the liver epithelial cells in the case of animals given single-cell bacterial protein with the feed.