Fabrication of a sensitive electrochemical sensor based on electrospun magnetic nanofibers for morphine analysis in biological samples.

A sensitive electrochemical sensor for detection of morphine (MPH) at the surface of electrode modified with electrospun magnetic nanofibers (MNFs) was prepared. The features of constructed sensor were evaluated by scanning electron microscopy (SEM), X ray diffraction (XRD) and electrochemical impedance spectroscopy (EIS). The modified sensor was used for MPH analysis using of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) method. The calibration curve has been composed of a linear portion in the concentration range of 0.0033-55 µM and 55-245 µM and the detection limit was 1.9 nM. The reproducibility of the peak current with a reliable relative standard deviation (RSD) value was acquired. Based on the results, the fabricated sensor has good stability and reproducibility, as well as the sensitive and selective analysis of MPH in human serum samples as real samples had effectively been feasible. The results of the actual sample were measured by HPLC procedure, and the results were compared with the results of the electrochemical method and corroborated them.